云南发展奶水牛业有潜力   总被引：1，自引：1，他引：0  

白文顺  毛华明 《中国奶牛》2012,(8):44-47

奶水牛业是我国奶业的第二大支柱产业,在南方地区发展奶水牛养殖,对于促进南方畜牧业发展,提高农民经济收入具有重要的战略意义。本文深入分析了云南奶业现状,从机遇、条件和优势等方面阐明了云南发展奶水牛业的潜力。  相似文献   

磷酸二酯酶抑制剂对水牛卵母细胞体外成熟的影响     

陈明  朱云干  卞桂华 《安徽农业科学》2012,(5):2724+2745

[目的]探讨磷酸二酯酶抑制剂米力农(milrinone)对水牛卵母细胞成熟的影响。[方法]水牛卵母细胞分成3组,分别在含0、50、100μmol/L的milrinone的成熟液中培养16 h后,每组的一半卵母细胞用来染色,检验核成熟情况;另一半卵母细胞再在不含milrinone的成熟液中培养12 h,挑选形态正常的卵母细胞受精后培养。[结果]添加50和100μmol/L milrinone的成熟液成熟的卵母细胞的生发泡破裂(GVBD)比率分别为25.9%和12.1%,显著低于对照组(76.9%);但另一半卵母细胞再培养、受精后试验组(50、100μmol/L mil-rinone)的分裂率(59.3%和61.2%)和囊胚率(35.9%和31.1%)与对照组(56.7%和28.8%)相比均无显著性差异(P0.05)。[结论]milrinone对水牛卵母细胞的成熟具有明显的抑制作用,成熟抑制解除后水牛卵母细胞仍可保持较高的发育潜能。  相似文献   

Clinical observations of cattle and buffalos with experimentally induced chronic copper poisoning     

Antonio H.H. Minervino  Raimundo A. Barrêto Júnior  Rodrigo N.F. Ferreira  Frederico A.M.L. Rodrigues  Selwyn A. Headley  Clara S. Mori  Enrico L. Ortolani   《Research in veterinary science》2009,87(3):473-478

The susceptibility of cattle and buffalos to chronic copper poisoning (CCP) was compared by using cattle (n = 10) and buffalo (n = 10) steers distributed into two copper supplemented (n = 6) and two control (n = 4) groups. Supplemented animals received 2 mg copper (Cu)/kg body weight daily for one week, with an additional 2 mg weekly until the end of the experiment (day 105). Three liver biopsies (day 0, 45, and 105) were obtained for mineral analyses; clinical examinations and blood samples were obtained every 15 days. Three supplemented cattle and two buffalos with typical manifestations of CCP died. There were no differences in the frequency of mortality between cattle and buffalos; hepatic copper concentration was higher in cattle than buffalos. These findings suggest that buffalos and cattle might be equally susceptible to CCP. However, buffalos accumulate less liver copper than cattle and have a lower threshold of hepatic Cu accumulation, which leads to clinical manifestation of CCP.  相似文献   

Fertility evaluation in Egyptian buffalo bulls using zona pellucida binding and in vitro fertilization assays     

A.M.H. Abdel Dayem  Karima Gh. M. Mahmoud  M.F. Nawito  M.M. Ayoub  T.H. Scholkamy 《Livestock Science》2009,122(2-3):193-198

This study aimed to develop a system of in vitro assays based on zona pellucida binding and in vitro fertilization for predicting male fertility in buffalo bulls. Frozen–thawed semen from nine bulls was tested for motility, viability index, acrosomal integrity, zona pellucida binding and in vitro fertilizing ability. Differences in post-thaw sperm motility between bulls were not significant. Differences in viability indices and percentage of spermatozoa with detached acrosome between bulls was highly significant (P < 0.001). Sperm attached per ovum, fertilization rates and polyspermy percentages varied significantly (P < 0.01) among buffalo bulls. A significant (P < 0.01) positive correlation coefficient of 0.69 was evident between normal acrosome and sperm attached per ovum, while between normal acrosome and fertilization efficiency it was 0.72. Sperm from different buffalo bulls differs in their ability to bind and fertilize oocytes. This study provides a basis to predict and maximize the in vitro fertilization performance of individual bulls.  相似文献   

奶水牛泌乳特性的研究综述     

朱柳  刘学洪 《中国奶牛》2011,(11):57-60

本文综述了国内外奶水牛泌乳特性的研究现状，从奶水牛的泌乳生理特性、泌乳规律及其泌乳潜力的激发等方面介绍了沼泽型水牛和河流型水牛及其杂交水牛的一些泌乳特征，并对今后水牛业的发展提出一些建议。  相似文献   

Ameliorative effects of boron on serum profile in buffalo (Bubalus bubalis) fed high fluoride ration     

Bharti VK  Gupta M  Lall D 《Tropical animal health and production》2008,40(2):111-116

An experiment was undertaken to evaluate the protective role of boron on the serum profile of buffalo calves fed a high fluoride ration. Twelve male Murrah buffalo (Bubalus bubalis) calves of 6–8 months age, divided into three groups of four calves in each, were fed basal diets and supplemented with sodium fluoride (NaF, 60 ppm) alone or in combination with borax (Na₂B₄O₇.10H₂O, 140 ppm) for 90 days. Boron (B) was added in the ration as borax to make @140 ppm boron (elemental B) on DM basis in treatment II. Dietary F caused a significant (p < 0.05) depressing effect on serum Ca and Zn on day 90 which was improved with B supplementation. However, serum Fe and Cu did not show any significant change on F or F+B supplementation. The serum ALP and phosphorus level were increased significantly (p < 0.05) on F feeding but declined significantly (p < 0.05) when B was fed. The findings suggested beneficial effect of boron on serum minerals and ALP in buffalo calves fed high fluoride ration.  相似文献   

Characterization of immunogenic proteins of Trypanosoma evansi isolated from three different Indian hosts using hyperimmune sera and immune sera   总被引：1，自引：0，他引：1  

Laha R  Sasmal NK 《Research in veterinary science》2008,85(3):534-539

The western blot analysis for identification of immunogenic proteins in whole cell lysate (WCL) antigens (Ags) prepared from the Trypanosoma evansi of buffalo, horse and cattle origins using hyperimmune sera (HIS) showed 11 immunogenic proteins and naturally T. evansi infected immune sera (IS) of horse detected 19 immunogenic proteins. HIS and IS of horse recognized five common immunogenic proteins of relative molecular weight (M(r)) ranges 61-64, 44-47, 33-34, 25-26 and 14-16 kilo Dalton (kDa). HIS rose against WCL Ags of T. evansi of buffalo origin and immune sera of horse cross reacted with WCL Ags of T. evansi of different host origin. It can be concluded that in comparison to HIS, IS of horse could able to detect more numbers of immunogenic proteins and five common immunogenic proteins in WCL Ages of T. evansi of different hosts origin. The evidence of higher reactivity of IS in comparison to HIS against T. evansi is being reported for the first time.  相似文献   

中国水牛遗传育种研究进展   总被引：1，自引：0，他引：1  

宋玉伟  张伟  赵高锋 《中国牛业科学》2006,32(4):38-40

我国水牛遗传育种的研究主要集中在染色体核型、蛋白质多态性、水牛胚胎体外生产与胚胎移植技术等方面。本文从中国水牛的类型和分布特征、遗传特性的研究现状、现代生物技术在水牛育种中的应用、中国水牛育种研究前景及展望等四个方面综述水牛的育种进展,认为以现代生物技术为核心的分子育种将成为水牛育种的总趋势,为我国水牛的产业化发展提供了理论依据。  相似文献   

三品种杂交水牛及其亲,子代染色体的研究   总被引：4，自引：0，他引：4  

黄右军  刘业基 《畜牧兽医学报》1989,20(2):123-128

三品种杂交水牛是尼里/拉菲×么拉×本地水牛的后代。应用染色体常规分析和G、C带技术,系统地研究三品杂水牛及其亲代和子代的染色体,结果表明,么拉、尼里/拉菲、本地水牛和么杂一代的染色体数目,分别是2n=50、2n=50、2n=48和2n=49,与前人报道相符。发现三品杂水牛及其横交子一代的染色体组型不一致、前者有2n=50和49两种,后者有2n=50、49和48三种。  相似文献   

Effect of NDF/undegradable crude protein ratio on in vivo digestibility, particle passage rate in riverine buffaloes compared with sheep     

S. Bartocci  S. Terramoccia 《Livestock Science》2006,104(1-2):38-45

In vivo digestibility (eight animals) and solid particles passage rate measured by faecal Cr (four animals fistulated at the rumen) were determined on riverine buffalo bulls and Delle Langhe rams, given four diets at maintenance level (50 g/kg M^0.75 per day of dry matter), according to a Latin square design, composed of a factorial combination of high and low NDF and of high and low protein undegradability. The diets were: L-30 = low NDF (530.0 g/kg DM), low undegradability of protein (29.6%, CP = 138.0 g/kg DM); L-40 = low NDF (537.0 g/kg DM), high undegradability of protein (41.0%, CP = 139.0 g/kg DM); H-30 = high NDF (583.0 g/kg DM), low undegradability of protein (28.3%, CP = 128.0 g/kg DM); H-40 = high NDF (NDF = 580.0 g/kg DM), high undegradability of protein (40.0%, CP = 128.0 g/kg DM). The digestibility of organic matter (66.68% vs. 64.32%, P < 0.05) and of the other analytical fractions (NSC, NDF, cellulose and hemicelluloses) was significantly higher in buffaloes with the exception of that of crude protein which was similar for the two species. Considering the diets within the species, the increased undegradable protein in the small intestine produces different effects: in the buffalo, it does not positively influence the digestibility of NSC but does increase that of cellulose, on the other hand in sheep it influences the digestibility of NSC. The post-ruminal digestibility of the undegraded protein, both in buffalo and sheep, is higher than that from protein of microbial origin. The passage rate of the marker of the solid particles, through the first compartment, k₁ (2.86% h^− 1 and 2.54% h^− 1 for the buffalo and the sheep) and through the entire intestinal tract, MRT (57.50 and 58.88 h for the buffalo and the sheep) does not show significant differences in the two species. The passage rate of the marker of the solid particles in buffalo rumen, at variance with the structural carbohydrates of the diet, is more variable compared with that of the sheep.  相似文献