特异PCR方法用于鸡的3种艾美耳球虫混合感染鉴别的研究     

郭远忠  翁亚彪  何芳  林瑞庆  李国清  朱兴全 《中国兽医杂志》2006,42(7):9-11

用单卵囊分离法获得的鸡的3种艾美耳球虫（每种各2株）卵囊：柔嫩艾美耳球虫（Eimeria tenella）、巨型艾美耳球虫（E．maxima）、堆型艾美耳球虫（E．acervulina）。经纯化、提取基因组DNA后，用报道的种特异引物做PCR扩增分析，以确定是否为纯种。结果发现这3种球虫均存在混合感染的情况。该结果为进一步研究这3种球虫奠定了基础，并说明特异PCR方法能够有效地、快速地鉴别球虫虫种。  相似文献   

细胞分裂素对红掌愈伤组织不定芽分化与增殖的影响     

王晶  牛瑞鹤  祁  伟等 《安徽农业科学》2014,(20):6542-6545,6602

[目的]研究细胞分裂素对红掌组培苗繁殖速度及质量的影响。[方法]以继代培养第6代的愈伤组织为试验材料,探讨了ZT、TDZ、6-BA 3种细胞分裂素及其浓度对不定芽分化及变异的影响。[结果]在使用浓度2.0~8.0 mg/L的范围内,随着细胞分裂素浓度的增加,正常不定芽的分化数及叶片数呈下降趋势,而异常不定芽分化逐渐增多,且随着培养时间的延长,这种影响变得更加明显;3种细胞分裂素的培养效果具有显著差异,ZT和6-BA添加浓度分别在2.0和4.0 mg/L以下时,其正常不定芽分化较多,不良变异率较少,而TDZ在0.5 mg/L时已显著抑制了正常不定芽的分化,同时易产生异常不定芽。[结论]3种细胞分裂素作用的强弱依次为TDZZT6-BA;在红掌愈伤组织继代培养诱导不定芽过程中,应避免使用高浓度细胞分裂素。  相似文献   

Characterization of Murine Pituitary-Derived Cell Lines Tpit/F1, Tpit/E and TtT/GF     

Saishu YOSHIDA  Masashi HIGUCHI  Hiroki UEHARU  Naoto NISHIMURA  Mitsuyoshi TSUDA  Hideji YAKO  Mo CHEN  Hideo MITSUISHI  Yoshiya SANO  Takako KATO  Yukio KATO 《The Journal of reproduction and development》2014,60(4):295-303

The pituitary is an important endocrine tissue of the vertebrate that produces and secretes many hormones. Accumulating data suggest that several types of cells compose the pituitary, and there is growing interest in elucidating the origin of these cell types and their roles in pituitary organogenesis. Therein, the histogenous cell line is an extremely valuable experimental tool for investigating the function of derived tissue. In this study, we compared gene expression profiles by microarray analysis and real-time PCR for murine pituitary tumor-derived non-hormone-producing cell lines TtT/GF, Tpit/F1 and Tpit/E. Several genes are characteristically expressed in each cell line: Abcg2, Nestin, Prrx1, Prrx2, CD34, Eng, Cspg4 (Ng2), S100β and nNos in TtT/GF; Cxcl12, Raldh1, Msx1 and Twist1 in Tpit/F1; and Cxadr, Sox9, Cdh1, EpCAM and Krt8 in Tpit/E. Ultimately, we came to the following conclusions: TtT/GF cells show the most differentiated state, and may have some properties of the pituitary vascular endothelial cell and/or pericyte. Tpit/F1 cells show the epithelial and mesenchymal phenotypes with stemness still in a transiting state. Tpit/E cells have a phenotype of epithelial cells and are the most immature cells in the progression of differentiation or in the initial endothelial-mesenchymal transition (EMT). Thus, these three cell lines must be useful model cell lines for investigating pituitary stem/progenitor cells as well as organogenesis.  相似文献   

雄性胎生蜥蜴腹鳞颜色的分化及其影响因素研究     

付晓宇赵文阁刘鹏刘志涛于东高智晟 《野生动物》2014,(1):107-112

同性间动物体色的变异是一种普遍现象,在自然选择和性选择中起到重要的作用,并与其采用的繁殖策略密切相关。为了检验雄性胎生蜥蜴腹鳞颜色的分化机制和影响因素,于2012年5—6月,选取黑龙江省大兴安岭地区的一个胎生蜥蜴种群进行实验。结果表明：该胎生蜥蜴种群雄性个体的腹鳞主要分为橙红、桔黄和淡黄3种颜色,且在个体形态上（除了前后肢和腹鳞数外）存在明显的差异,推测腹鳞颜色的分化与年龄有关。温度和光照对腹鳞颜色具有间接的影响,在低温或无光条件下,3种颜色的雄性胎生蜥蜴的体重显著下降,腹鳞的颜色变暗。在此基础上,讨论了雄性胎生蜥蜴腹鳞颜色作为一种通讯信号所具有的潜在生态学意义。  相似文献   

美花石斛原球茎增殖及分化的研究   总被引：2，自引：0，他引：2  

包英华  白音  何清清 《安徽农业科学》2007,35(3):675-677

将美花石斛原球茎培养于含有不同生长调节物质的SH培养基上,结果发现,不同生长调节物质对美花石斛原球茎分化的不同阶段产生不同的影响.含有香蕉提取物和BAP2.0 mg/L+KT2.0 mg/L+NAA 1.0 mg/L的SH培养基对鲜重增加影响较显著;白萝卜汁对叶片分化有一定的促进作用;含有酵母提取物的SH培养基对增殖芽和不定根分化效果最好;马铃薯、香蕉和椰子汁对生根分化影响较大.建议在美花石斛原球茎不同分化阶段,根据其生长和分化需要,继代培养于相应的培养基上,以获得优质美花石斛组培苗.  相似文献   

小麦幼胚胚性愈伤组织诱导和再分化研究   总被引：5，自引：0，他引：5  

赵元增  茹振钢  李淦 《河南农业科学》2006,(2):31-34

以4个小麦品种的幼胚为外植体,通过脱分化和再分化培养,研究了影响小麦幼胚愈伤组织诱导和再分化的因素。结果表明:小麦幼胚胚性愈伤组织的诱导和再分化主要受基因型的限制,同时也受培养基组成的影响;培养基中添加0.5mg/L的ABA不利于胚性愈伤组织的诱导和分化;胚性愈伤组织诱导率和幼苗分化率之间呈极显著正相关,提高胚性愈伤组织的诱导率和形成量是建立小麦植株再生体系的关键。  相似文献   

重瓣铁线莲愈伤组织诱导研究   总被引：2，自引：0，他引：2  

张涛  周琼  张丽琼  屈国胜  廖咸康 《安徽农业科学》2008,36(22)

[目的]为运用现代生物技术开发利用重瓣铁线莲提供参考。[方法]以重瓣铁线莲的茎段为外植体,在MS基本培养基中添加0.2mg/L IAA,0.5、1.0、1.52、.0 mg/L的6-BA和0.1、0.2、0.4、0.6、0.81、.0 mg/L的2,4-D,观察重瓣铁线莲愈伤组织的诱导情况,统计诱导率。[结果]各处理诱导出的愈伤组织均为致密型愈伤组织,很少进行分裂分化,故在增殖培养中需提高植物生长物质的浓度。培养基MS+1.0 mg/L 6-BA+0.8 mg/L 2,4-D+0.2 mg/L IAA的诱导效果最好,不仅形成的愈伤组织量多,而且诱导率高达90.0%。在重瓣铁线莲愈伤组织诱导中,植物生长物质6-BA、2,4-DI、AA的适宜比例为5∶4∶1。[结论]重瓣铁线莲茎段愈伤组织适宜的诱导培养基为:MS+1.0mg/L 6-BA+0.8 mg/L 2,4-D+0.2 mg/L IAA。  相似文献   

胚胎干细胞体外培养研究进展及设想     

田文霞 《山西农业大学学报(自然科学版)》2006,26(3):226-229

胚胎干细胞具有高度复制能力及多向性分化潜能,近年来由于胚胎干细胞的分离、培养技术的提高,尤其是对干细胞表面分子标记和诱导分化研究的深入,胚胎干细胞展示出很好的临床应用前景,同时也将为基因功能研究、药物研究提供良好的工具。但对基因组印迹、免疫排斥及干细胞的有效定向分化仍然是亟待解决的问题。  相似文献   

Allozyme Variation Among Some Pearl Millet (<Emphasis Type="Italic">Pennisetum glaucum</Emphasis> L.) Cultivars Collected from Tunisia and West Africa     

Oumar Ibrahima  Farhat Chibani  Sawsen A. Oran  Mohamed Boussaid  Yannis Karamanos  Aly Raies 《Genetic Resources and Crop Evolution》2005,52(8):1087-1097

Allozyme variation was assessed in a collection of pearl millet cultivars derived from three different regions of traditional cultivation of this cereal in Tunisia and West African countries. Using starch gel electrophoresis, 11 loci corresponding to seven enzyme staining systems were identified. Important within-cultivar genetic diversity was detected. Genetic diversity within Tunisian cultivars was similar and was more pronounced than for West African ones. This result could be explained by the low level of the human selection characterizing the Tunisian cultivars. Most of the total genetic diversity is maintained within subregions suggesting little genetic differentiation. Cluster analysis did not reveal separate clusters for the various subregion cultivars. However the cluster analysis should help in the selection of interesting genotypes for future breeding programs.  相似文献   

Phylogenetic differentiation of cultivated rice,XXIII. Potentiality of wild progenitors to evolve the Indica and Japonica types of rice cultivars     

Hiko-Ichi Oka  Hiroko Morishima 《Euphytica》1982,31(1):41-50

Summary Studies of intermediate wild-cultivated plants suggested that the Indica-Japonica differentiation of cultivars of Oryza sativa L. has taken place with the domestication of wild plants. The wild progenitor, which is considered to be the Asian form of O. perennis Moench, shows no trace of differentiation into two such types as the Indica and Japonica. To elucidate the potentiality of the wild progenitor to evolve the two types when domesticated, the selfed progenies from an Indica × wild and a japonica × wild cross were examined with regard to three characters known to be useful for distinguishing between the two types. The data showed that Japonica-like plants were derived from the Indica × wild cross and Indica-like plants from the Japonica × wild cross. The Indica-Japonica differentiation was considered to have resulted from selection of incipient domesticates having such potentiality under different climatic conditions; the dynamics was discussed.  相似文献