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21.
为探究非洲猪瘟病毒(African swine fever virus,ASFV)标准物质作为试剂盒评价体系的可行性,比较了市场上5种主流品牌ASFV荧光PCR检测试剂盒的检测性能。使用ASFV P72基因核酸标准物质作为模板,根据5种试剂盒说明书分别进行相应的荧光定量PCR检测,结合扩增曲线、Ct值,分析不同试剂盒的敏感性、可重复性以及所需反应时间。结果显示:5种试剂盒的阴性、阳性对照均成立,最低检测限均为5.9×10-1拷贝/μL;4个厂家的试剂盒线性关系R2>0.98,其中最优的R2=0.994,离散度最小;各试剂盒的实际反应耗时与理论反应耗时均有一定差异(0.20~0.96 h)。结果表明,各生产厂家使用P72基因作为靶基因研制的ASFV荧光PCR检测试剂盒都可以使用ASFV标准物质作为评价体系,来评判试剂盒的检测性能。本试验为各实验室不同样品检测的ASFV荧光PCR检测试剂盒选择提供了一种可用的评价方法。 相似文献
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为持续控制小麦叶锈病及促进小麦的抗叶锈病育种工作,2019—2020年自江苏、浙江和安徽3个省采集自然感叶锈病的小麦病叶,经分离获得小麦叶锈菌单孢分离物,利用43个小麦叶锈病鉴别寄主材料对其致病类型进行鉴定,并对其毒性结构进行分析。结果显示,从170份小麦叶锈菌单孢分离物中共鉴定出67个致病类型,主要致病类型为THS、SHJ、PHS和SHS,出现频率分别为8.8%、7.6%、5.9%和5.9%。江苏、浙江和安徽3个省的单孢分离物对携带抗叶锈基因Lr10、Lr12、Lr22a、Lr22b、Lr29、Lr33、Lr35和Lr36的鉴别寄主材料的苗期毒性频率均超过90.0%,而对携带抗叶锈基因Lr9、Lr24、Lr25、Lr28、Lr38、Lr40、Lr41、Lr42、Lr43和Lr13+3ka的鉴别寄主材料的苗期毒性频率均小于10.0%。卡方检验及Fisher精确检验显示,3个省小麦叶锈菌群体对抗叶锈基因Lr1、Lr2a、Lr3、Lr14b、Lr18、Lr21、Lr26、Lr27+31、Lr32和Lr37的毒力存在显著分化。浙江省小麦叶锈菌群体具有较少的毒性因子（4.73）和毒性值（600... 相似文献
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三步合成了烷基-二醇基硅胶(ADS)色谱材料，首先用γ-缩水甘油醚氧丙基三甲氧基硅烷把功能基团键合到硅胶基体上，再把该环氧化硅胶与硬脂酸反应以制备酯型十八烷基反相填料，最后在20MPa压力下装成色谱预柱(35×4.6mm I.D)。用该预柱通过六通阀后接ODS分析柱(250×4.6mm I.D)，构成中心切割二维HPLC。该系统能对具有一定疏水性的化合物进行在线富集，实现环境及生物复杂样品直接进样分离分析。使用该系统对尿中的莠去净和西玛津残留进行了分离分析，以含有0.1%乙酸的酸性水缓冲液对加标尿液中莠去净类化合物保留在预柱上，干扰分析的大分子被排阻而无保留通过，其它干扰分子保留小而通过，再切换六通阀，以乙腈和0.1%乙酸水溶液进行梯度洗脱，进而在下一级ODS分析柱上进行分离（紫外检测225nm），两种除草剂的分离分析取得了良好的定性定量结果 相似文献
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In accordance with the special characteristics of state medium-sized construction enterprises and their reform objects of "having a free hand and enlivening", the suitable objective model, the stock cooperative system for state medium-sized construction enterprises has been put forward in this paper by analyzing the system mechanism of stock cooperative system enterprises. Then the causes for it were expounded. 相似文献
28.
A new type of inorganic anchoring material is developed as a substitution for anchoring adhesive to overcome some imperfections in the process of planting reinforcing steel bar.The mix proportion of the anchoring material is determined by experiments.The compressive strength,adhesive force to concrete,volume stability and anchoring force with steel bar are studied.The early strength of the anchoring inorganic material is quite high: for 1d up to 38.5MPa and 3d up to 60MPa.It is a good adhesive to concrete and the adhesive strength of 28d is about 2MPa.The anchoring material will have tiny volume expansion with the age,which is beneficial to enhancing the anchoring force.The essential component of the inorganic anchoring material is cement-based material,so some defects of the organic macromolecular material can be avoided,such as poor stability and requirement for vigorous construction conditions.Its characteristics in early strength,adhesive force to concrete and volume expansion can satisfy the requirement of the construction to the planting reinforcing steel bar and the loading force,thus it can be used as a substituting material for anchoring adhesive. 相似文献
29.
Geogrid reinforced soil retaining wall(GRSRW) is composed of panel,geogrid and soil,its coupled hydromechanical structural characteristics are increasingly emphasized in practice.In this paper,based on the characteristics of the reinforcement-soil interaction,the grogrid and soil around it with a definite thickness were regarded as a kind of composite material with strain-compatible condition.A saturated porous media model was introduced to simulate the soil and reinforcement-soil composite material hereby and the mathematic model for numerical analysis on structural characteristics of GRSRW was built up.The FEM equations were deduced from the standard Galerkin procedure,resolving these equations,we can gain the relevant dynamic response of saturated GRSRW. 相似文献
30.
Pearlitic steel is composed of numerous pearlitic colonies with randomly distributed orientations,and each colony is composed of many alternatively arranged parallel lamellas of ferrite and cementite.The pearlitic steel with fine interlamellar spacing possesses excellent mechanical properties,such as high strength,good resistance against wear,and high fatigue life,etc. Based on the inconsistency of the deformation between the two phases,a strain_equivalent and non_local constitutive model is proposed. Using this model,the effect of the interlamellar spacing of peailitic steel,a fundamental parmeter of the microstructure of pearlitic material, on its macroscopic mechanical behavior can be described without changing the local material parameters for each phase of the material.The asymmetrical cyclic plasticity of both the hot_rolled and the off_line full_length quenched pearlitic steel PD3 is analyzed.The comparison between the computational and the experimental results shows satisfactory agreement. 相似文献