马流感病毒(A/equine/Qinghai/1/94)核蛋白基因的序列测定及同源性分析   总被引：1，自引：0，他引：1  

杨建德  薛飞  王晓钧  朱远茂  赵立平  吕晓玲  沈荣显  相文华  李景鹏 《中国预防兽医学报》2003,25(2):96-98

根据已发表的马流感病毒核蛋白基因序列，设计并合成一对特异性引物，经反转录-聚合酶链反应（RT-PCR）成功扩增出了我国马流感病毒（A/equine/Qinghai/1/94核蛋白基因。将该片段连接到PGEM-T-EASY载体并转化DH5α，提取阳性菌落的质粒径EcRo1酶切的PCR鉴定其大小为1.5kb左右，对其测序并进行分析发现，与A/Equine/Kentucky/2/86,A/Equine/Miami/1/63等关系较近，同源率为93.3%--93.4%，而与我国马流感吉林A/Equine/Jilin/1/89株关系较远，同源率仅为84.6%。  相似文献   

柑桔树体性状与产量的相关研究     

刘远鹏  曾伟光 《四川农业大学学报》1985,(1)

通过对血橙、温州蜜柑的调查和统计分析,探明了柑桔冠径、千周、叶面积系数和末级梢数对幼树早期产量有极显著影响,其中冠径、干周是早期丰产稳产的重要树体结构基础。树冠投影面积和复盖率与群体产量的关系较密切,二者共同对产量作用的决定程度最大,又互为制约和消长。为早果丰产栽培提供了依据。  相似文献   

Alterations in flower and seed morphologies and meiotic chromosome behaviors of micropropagated rhubarb (Rheum rhaponticum L.) ‘PC49’     

Yipeng Zhao  Yan Zhou  Brian W.W. Grout 《Scientia Horticulturae》2008

Variations in flower and seed and meiotic chromosome behaviors were investigated in micropropagated rhubarb (Rheum rhaponticum L.) ‘PC49’. The results showed that micropropagated plants had significantly higher variations in flower and seed morphologies and chromosome behaviors than plants derived from crown division. The results demonstrated that micropropagated plants grew 2–5 stamens, 8–13 stigmas, and 2–7 seed-wing, compared to 3 stamens, 9 stigmas and 3 (rarely 4) seed-wing in crown-derived plants. Additionally, alteration in chromosome behaviors (i.e. lagging and bridging) was observed only in micropropagated plants. All the results suggest that somaclonal variation may have been involved in micropropagated rhubarb ‘PC49’.  相似文献   

禽流感病毒NP蛋白单克隆抗体的制备与鉴定     

黄小洁 《中国兽药杂志》2023,57(6):10-16

为获得禽流感病毒(Avian influenza virus, AIV)NP蛋白的单克隆抗体,将构建的重组表达质粒pET-30a-NP转化BL21细胞,经IPTG诱导表达、纯化后作为免疫原,免疫8周龄Balb/c雌性小鼠,并按常规方法制备杂交瘤细胞。通过ELISA方法、Western-blot方法进行筛选,获得3株杂交瘤细胞株,命名为3A6、2H12、5F7,并进行了培养特性、分泌抗体活性、分泌抗体亚类的鉴定。结果显示：3株细胞株连续传10代均稳定分泌单克隆抗体,分泌的单克隆抗体亚型均为IgG2b,轻链类型均为Kappa。制备并纯化了以上3株单克隆抗体,浓度分别为2.9、2.5、2.8 mg/mL,纯度不低于90%。West-blot检测,单抗与H7血凝抑制试验抗原、H5血凝抑制试验抗原、H9N2病毒能发生特异性反应,说明单抗具有广谱性,且与IBDV、REV、IBV、MDV、ALV、AE、ILT、NDV、EDSV等均无特异性条带出现,说明特异性良好。本研究制备的3株针对禽流感NP蛋白的单抗,具有较好的特异性、保守性和广谱性,为下一步开展AIV诊断试剂如IFA检测试剂盒、ELISA检测试...  相似文献   

甜玉米新品种晋单(甜)49号的选育     

苗如意  岳青  阎继耀  张培忠  赵安全  马海龙  智海英 《中国蔬菜》2005,(10):119-120

晋单(甜)49号是以自交系GT193为母本,自交系GT195为父本选育而成的一代杂种.株型清秀,出苗到采收鲜穗约90 d(天).叶片较窄,平展而细长,略下披,节间较长.株高260 cm左右,穗位高约100cm.穗长约22.0 cm,粗4.7 cm,穗行数14～16行,每行40粒,籽粒浅黄色,果皮薄嫩,口感好,鲜穗产量约1 000kg·(667m2)-1.高抗矮花叶病毒病、粗缩病、穗腐病,全生育期需有效积温2 100～2 400℃,适宜南北方春播和夏播种植.  相似文献   

本地毛形线虫49 ku ES重组蛋白诱发小鼠保护性免疫的研究     

韩周  姜曰晓  李继红  肖文左  宋铭忻  路义鑫 《中国兽医杂志》2008,44(2):6-8

目的研究本地毛形线虫肌幼虫49ku ES重组蛋白对小鼠的免疫保护作用。方法以表达的本地毛形线虫肌幼虫49ku ES重组蛋白免疫小鼠,共免疫3次。末次免疫后10d,每只小鼠攻击感染200条本地毛形线虫感染性肌幼虫,检测本地毛形线虫7日龄成虫数、雌虫体外产新生幼虫数以及感染35d肌幼虫数,并且计算减虫率,应用自制的ELISA方法测定各组小鼠血清抗体OD值。结果成虫减虫率、新生幼虫减虫率、肌幼虫减虫率分别为9.4%、70.2%和71.3%,免疫组血清抗体水平远远高于佐剂对照组和感染对照组（P〈0.01）。结论本地毛形线虫肌幼虫49ku ES重组蛋白诱导小鼠产生较好的抗本地毛形线虫的保护性免疫。  相似文献   

H5N1亚型禽流感病毒核蛋白基因的克隆及分子进化分析   总被引：1，自引：0，他引：1  

王振国  金宁一  马鸣潇  张洪勇  郑敏  尹革芬 《吉林农业大学学报》2004,26(3):326-329

用RT—PCR法,以1株H5N1。亚型禽流感病毒RNA为模板,扩增了NP全基因cDNA。将NP cDNA克隆于pMD18-T载体,并对其进行测序。测序结果表明：所克隆的NP基因全长1542bp,该核苷酸的片段包含了核蛋白完整阅读框架,其中编码区为1497个核苷酸,编码498个氨基酸。将其序列与6株A型流感病毒NP基因序列进行比较,各毒株之间NP基因核苷酸序列同源性为92．5％～95．9％,编码的氨基酸同源性为97．0％～98．4％。通过分子进化分析揭示了各毒株间的亲源关系。  相似文献   

Soil microbial biomass and activity response to repeated drying-rewetting cycles along a soil fertility gradient modified by long-term fertilization management practices   总被引：3，自引：0，他引：3  

Bingzi Zhao  Ji Chen  Jiabao Zhang  Shengwu Qin 《Geoderma》2010,160(2):218-224

The effects of repeated drying-rewetting (DRW) cycles on the microbial biomass and activity in soils taken from long-term field experiment plots with different fertilization (FERT) management practice histories were studied. We investigated the hypothesis that soil response to DRW cycles differs with soil fertility gradient modified by FERT management practices. The soils were incubated for 51 days, after exposure to either nine or three DRW cycles, or remaining at constant moisture content (CMC) at field capacity. We found that both DRW and FERT significantly affected soil properties including NH₄-N, NO₃-N, dissolved organic C (DOC), microbial biomass C (Cmic), basal soil respiration rate (BSR), urease activity (URE) and dehydrogenase activity (DHD). Except for NH₄-N and BSR, variation in the properties was largely explained by FERT, followed by DRW, and then their interaction. Irrespective of the soils' FERT treatment, repeated DRW cycles significantly raised the DOC and Cmic levels compared with CMC, and the DRW cycles also resulted in a significant decline in BSR and URE and increase in DHD, probably because the organisms were better-adapted to the drying and rewetting stresses. The variations in soil biological properties caused by DRW cycles showed a significantly negative relationship with the soil organic C content measured prior to the start of the DRW experiments, suggesting that soils with higher fertility are better able to maintain their original biological functions (i.e., have a higher functional stability) in response to DRW cycles.  相似文献   

"NP1+格助词+の+NP2"中格助词的显隐机制     

张佩霞 《湖南农业大学学报(自然科学版)》2006,(4)

日语里“NP1 格助词 の NP2”中格助词的显陷模式可分为必须无形化、转化为其他格助词、必须保留、可省略四类,其制约机制可求助于“格的阶层性”理论,即在“格的阶层”中处于上位的格助词必须或容易无形化。  相似文献   

四川紫色土和黄壤中铬和砷两种元素背景值的判定     

刘守恒  王泽洲 《四川农业大学学报》1984,(2)

<正> 紫色土是在紫色岩风化物上发育形成的土壤。紫色岩是侏罗纪和自垩纪时期厚度较大的湖积物,其风化特点以物理风化为主,化学风化弱,因此,风化物的性质与母岩相近。形成的土壤肥沃,富含磷、钾及微量元素,是四川省的主要农业耕作土壤。多分布于全省低中丘陵地形区域。黄壤是湿润亚热带地区的地带性土壤,四川省的黄壤主要分布于盆地西部台地  相似文献