不同肥料处理对盆栽红豆杉生长的影响     

李敏  张健  李玉娟  谈峰  丛小丽  顾建华 《江西农业学报》2010,22(10)

研究了不同肥料处理对盆栽红豆杉的茎高、新枝、根长和鲜重的影响。结果表明:每周对红豆杉叶面喷施3‰磷酸二氢钾,对红豆杉的生长效果最好。  相似文献   

含LacZ重组逆转录病毒的制备及其在NIH3T3细胞的表达   总被引：2，自引：0，他引：2  

王凤阳  孟庆文  扈荣良  池海英  张茂林  于康震  殷震 《中国预防兽医学报》2001,23(5):329-331

构建了含有3．7kbLacZ的重组逆转录病毒载体,转染包装细胞系PA317后,经G418筛选,得到了G418的抗性的产毒细胞系,用收获的含有重组逆转录病毒粒子的浓缩病毒上清,感染NIH3T3细胞。经X-gal染色检测,发现表达了LacZ的NIH3T3细胞呈蓝色。  相似文献   

Identification of Trichoderma SKT-1, a biological control agent against seedborne pathogens of rice     

Satoshi Watanabe  Kazuo Kumakura  Hideki Kato  Hiroyuki Iyozumi  Masayuki Togawa  Kozo Nagayama 《Journal of General Plant Pathology》2005,71(5):351-356

Trichoderma SKT-1 was previously reported as a powerful biological control agent against seedborne pathogens of rice, but the taxonomic disposition of the fungal isolate was not clear. Trichoderma SKT-1 produced irregular pyramidal warts on conidia and had an optimum growth temperature of 30°C. Morphological characteristics and colony growth were identical to those of known species of Trichoderma, including the newly recognized species T. asperellum. The 5.8S rDNA with the internal transcribed spacer (ITS) region (ca. 514 bp) of the fungus was compared with those of known species to determine the phylogenetic placement of the fungus. The length and sequence of the regions from Trichoderma SKT-1 were completely identical to those of an isolate of T. asperellum NRRL 5242 (AJ230669). On the basis of these results, we concluded that Trichoderma SKT-1 was T. asperellum.  相似文献   

O型口蹄疫病毒VP1 T细胞与B细胞表位基因双拷贝串联表达产物的免疫应答   总被引：1，自引：0，他引：1  

庄娟  曹祥荣  陈波  饶忠  潘洁  徐泉兴  尤永进 《中国预防兽医学报》2005,27(6):494-497,503

以一株O型口蹄疫病毒(FMDV)外壳蛋白VP1基因为模板,合成与细胞免疫及体液免疫相关抗原表位肽基因:21-40肽(20AA)和141-160肽(20AA)基因序列,运用基因工程技术构建了含有串联结构21-40(20AA)～141-160(20AA)～21-40(20AA)～141-160(20AA)的2020-2020VP1融合基因表达载体r2020-2020,转化宿主菌BL21(DE3)RIL后诱导表达,表达产物经SDS-PAGE及Western Blot分析显示重组融合蛋白的分子量约为18Ku.动物实验表明,较小剂量的融合蛋白就能诱导豚鼠产生特异性T淋巴细胞增殖反应及抗FMDV中和抗体,证明该融合蛋白可同时激活细胞免疫及体液免疫反应,具有开发成为抗FMDV疫苗的应用价值.  相似文献   

中草药免疫增强剂对鸡小肠T淋巴细胞免疫组化研究   总被引：1，自引：1，他引：1  

史秋梅  段玲欣  沈萍  汤生玲  范莉  张艳英 《中兽医医药杂志》2005,24(4):5-7

为了探讨中草药免疫增强剂的作用机理,采用组织学常规切片技术和免疫组织化学染色的方法对鸡小肠黏膜淋巴组织中T淋巴细胞的数量分布进行了动态观察.将1日龄60只健康公鸡随机分成2组,一组为对照组,另一组为试验组,试验组按1%浓度的中草药免疫增强剂饮水,连续21 d.在第14天、第21天分别取各组鸡小肠,制成组织切片,进行组织学观察.结果显示,试验组鸡小肠的T淋巴细胞数显著高于对照组(P<0.05),表明中草药免疫增强剂对鸡小肠免疫有显著的增强作用.  相似文献   

中药复方总多糖及其含药血清对小白鼠免疫功能的影响   总被引：2，自引：0，他引：2  

邵永斌  谷新利  罗燕  赵宗胜  李炳奇 《中兽医医药杂志》2005,24(6):8-11

分别给小鼠灌服复方总多糖、复方水煎剂、黄芪多糖、生理盐水后制备含药血清,用MTT法测定小鼠淋巴细胞增殖程度,用酶法一氧化氮试剂盒测定腹腔巨噬细胞一氧化氮的生成量,同时与各种药物的体外实验相比较.结果显示,复方总多糖及其含药血清均能促进小鼠脾脏淋巴细胞增殖和腹腔巨噬细胞一氧化氮的生成量,且效果优于复方水煎剂和黄芪多糖.  相似文献   

Effects of N-methyl-D, L-aspartate on secretion of growth hormone-releasing hormone from the boar hypothalamic-preoptic area and median eminence in vitro     

Estienne MJ  McElwain KV  Barrett JB  Barb CR 《Domestic animal endocrinology》1999,17(4):1156-407

N-methyl-D, L-aspartate (NMA) elicited secretion of growth hormone (GH)-releasing hormone from both the hypothalamic-preoptic area and the median eminence that were collected from boars. We suggest that the previously described increase in GH secretion that follows peripheral treatment of swine with NMA is attributable, at least in part, to NMA-stimulated secretion of GH-releasing hormone from the central nervous system.  相似文献   

伊氏锥虫弱毒株免疫家兔的T细胞应答反应     

王景林  刘俊华 《中国兽医学报》1991,(3)

以ANAE染色法和~3H-TdR掺入的淋巴细胞转化试验,测定了伊氏锥虫弱毒株免疫家兔的T细胞动态变化.结果,免疫兔外周血T细胞增殖反应在免疫后第24d达到高峰,ANAE阳性细胞百分率这72.65%,对照组仅为41.2%;约1周后,出现抑制性T细胞,其增殖反应也明显减弱,最后完全被抑制.而外源性白细胞介素2的加入,可解除这种免疫抑制.攻虫后,特异性T细胞应答反应未再出现强烈的回忆反应,但伊氏锥虫弱毒株免疫家兔获得9/12的保护.因此,T细胞在这种抗锥虫免疫中,没有起到主导作用.  相似文献   

Failure to induce mucosal disease in cattle persistently infected with bovine viral diarrhea virus by treatment with adrenocorticotropic hormone     

B Larsson 《Acta veterinaria Scandinavica》1988,29(1):1-8

Recent research has shown that cattle that develop mucosal disease (MD) often, if not always, have been persistently infected with bovine viral diarrhea virus (BVDV) since birth. The purpose of the present study was to determine whether MD could be induced by immunosuppression of persistently BVDV-infected cattle. For that purpose, adrenocorticotropic hormone (ACTH) was injected intramuscularly, twice daily for 5 consecutive days in 4 persistently BVDV-infected cattle and in 3 control cattle. Before the ACTH treatment, the numbers of leukocytes, neutrophils and mononuclear cells (MNC) per litre of blood in BVDV-infected cattle were in the same range as in the controls. Similarly, the proportions of B cells, T cells, monocytes and Fcγ⁺ cells (cells with receptor for the Fc part of IgG) were the same in the 2 groups of animals. On the other hand, the proliferative response to mitogen stimulation of MNC obtained from the control animals was twice as high as the corresponding value of the persistently BVDV-infected cattle.In all animals, ACTH treatment caused increased Cortisol concentrations, leukocytosis, neutrophilia and decreased mitogen-induced lymphocyte stimulation. However, the MNC count and the proportions of B cells, T cells, Fcγ⁺ cells and monocytes remained unaltered. In spite of the immunosuppression, indicated by the decrease in mitogen-induced lymphocyte stimulation. ACTH treatment did not provoke any clinical signs of MD in the persistently BVDV-infected cattle.  相似文献   

In vitro blastogenic responses of peripheral lymphocytes from marmosets to phytohemagglutinin and to autologous lymphocytes transformed by Epstein-Barr virus     

S K Sundar  P K Lai  Z H Bengall  J L Cicmanec  K Goldstein  P H Levine 《Comparative immunology, microbiology and infectious diseases》1981,4(1):35-45

White-lipped marmosets were evaluated for their cell mediated immune (CMI) response to EBV to determine the feasibility of CMI studies in marmoset models for EBV oncogenesis. The mitogen, cell concentrations, the length of incubation period and serum requirements were defined for in vitro lymphocyte stimulation tests. The level of response of each animal was dependent on the concentration of phytohemagglutinin-P (PHA-P) and was independent of cell densities employed. The rate of tritiated-thymidine incorporation by mononuclear cells due to PHA-P increased exponentially between 2–4 days. This test was reproducible for a given batch of PHA-P when the cells were cultured in the presence of 10% heat inactivated fetal bovine serum. The five white-lipped marmosets were seronegative for EBV antigens and did not show lymphocyte stimulation with EBV particles and EBV soluble antigen, but two of these animals exhibited significant stimulation with autologous lymphocytes transformed in vitro by B95-8 virus. Despite the limited amount of blood (3–4 ml) that could be obtained from each animal in a single bleeding, it was possible to perform multiple lymphocyte stimulation assays with the protocol used.  相似文献