Phenotypic and genetic characterization of Erwinia carotovora from mulberry (Morus spp.)     

S. T. Seo  N. Furuya  C. K. Lim  Y. Takanami  K. Tsuchiya † 《Plant pathology》2003,52(2):140-146

Phenotypic and genetic characteristics of nine bacterial strains isolated from mulberry ( Morus spp.), which were originally described as Erwinia carotovora ssp. carotovora (Ecc), were investigated. Based on the results of biochemical tests, these bacterial strains were divided into two different types, type 1 and type 2. Two strains of type 1 were similar to Ecc, whereas seven strains of type 2 were distinct from Ecc. A polyphasic study that included serological assay, specific PCR assay for E. carotovora ssp. atroseptica (Eca), PCR-RFLP of a pectate lyase ( pel ) gene and RAPD-PCR was performed on the type 2 strains, and the data were compared with those of related E. carotovora subspecies. The results of serological and specific PCR assays for Eca showed that the type 2 strains were distinct from Eca. In RFLP analysis of the pel gene using Sau 3AI, the type 2 strains showed a unique RFLP pattern. On the basis of RAPD analysis, similarity of RAPD patterns within the type 2 strains was very high. A unique RAPD fragment was isolated from the type 2 strains and used as a probe for Southern hybridization. This probe hybridized only with PCR products from the type 2 strains. Based on phenotypic, serological and genetic characteristics, the type 2 strains isolated from mulberry may belong to a distinct E. carotovora subspecies other than Eca or Ecc.  相似文献   

茭儿菜(野茭白)的生物学特性   总被引：3，自引：0，他引：3  

王长春  杨德海  钱芝龙  潘宝贵 《中国蔬菜》2004,1(5):9-11

茭儿菜 ,别名野茭白、野茭瓜 ,属禾本科菰属。须根系 ,茎分地上茎和地下茎 ,叶分叶鞘和叶片两部分 ,圆锥状花序 ;茭儿菜在春季发芽、生根 ,随温度升高生长加快 ,夏、秋季抽穗、开花、结实 ;以地下匍匐茎和地上茎作为繁殖材料 ,生长发育对环境条件的要求 :适温范围 10～ 2 8℃ ,生长期间不能缺水 ,需 2 5～ 5 0cm深厚土层 ,以粘壤土或壤土为宜 ,生长期间需要充足的阳光 ,不耐遮荫  相似文献   

中国野牦牛种质资源库体系的建立及利用     

杨博辉  姚军  郎侠  阎萍  梁春年  孙晓萍  郭健  郭宪  程胜利 《中国草食动物》2005,25(6):34-36

介绍构建野牦牛种质资源信息收集、整理及调查的方法;建立了野牦牛50头规模的可良性运作的遗传繁育基地，可年产2万支冷冻精液的动态保存库，以及0.1万～0.15万枚野牦牛冷冻胚胎动态保存库的条件与规模;创建了国内外相关野生动物保护组织、行业、协会等保护和利用野牦牛遗传资源的共享平台.  相似文献   

Analysis of High Reproductivity on Wild Yak Bull     

陆仲璘  J.W.ZHANG 《中国草食动物》2005,(Z2):199-200

Sperm concentration of wild yak bull is 2. 13 billion per milliliter. Survival time at 0-4 ℃ is 57hrs. After thawing survival time at 37 ℃ is 12 hrs. Resistance coefficient is 144,000. Abnormal sperm rate and acrosomal integrated rate of post-thawing is 9.17% and 87.53% respectively. Moving viscosity is 1. 169 cp. Total nitrogen is 1 437.7 mg/100 ml. Head of sperm is significantly shorter and end piece is significantly longer than that of domestic yak and yellow cattle. Activity of hyaluronidase is highly stronger than the domestic yak,and the activity of LDH is higher than the domestic yak by 48 %. Hence,the fertility of the wild yak bull is powerful and the artificial inseminated rate with domestic yak and yellow cattle is 88.9% and 71.58% respectively.  相似文献   

广州石门国家森林公园野生观赏植物资源调查     

李胜强  崔杰 《广东园林》2015,(3)

通过野外调查和查阅文献资料,得出石门国家森林公园共有野生观赏植物122科267属377种,其中蕨类植物19科23属28种,裸子植物4科5属7种,被子植物99科239属342种。分析了石门国家森林公园野生观赏植物资源的基本特征和用途,并探讨其保护和开发利用。  相似文献   

值得在华南地区推广应用的3种野生观赏樱属植物   总被引：2，自引：1，他引：1  

叶超宏  胡晓敏  刘湘源  张丹丹  叶小玲  李仕裕 《广东园林》2015,(3)

通过对华南地区野生樱属植物进行深入的调查,从形态特征、生态习性、繁殖、观赏价值、园林应用等方面,介绍了钟花樱桃、尾叶樱桃和海南樱桃3种适合在华南地区推广应用的樱属植物。  相似文献   

桑叶养猪实用技术     

肖和良 《猪业科学》2020,37(2):76-79

桑叶养猪实用技术分为桑树种植技术和桑叶干粉发酵养猪技术两个部分。利用荒地种植桑树,降低生猪养殖饲养成本,桑叶养猪种养结合,成为生态农业典范。桑叶干粉发酵技术,提高了桑叶干粉的可消化率和饲料功能。桑叶干粉发酵料占配方6%~10%,做成桑叶猪饲料,替代3%~5%鱼粉、豆粕等蛋白质饲料,节约了饲料成本。桑叶喂养猪(简称桑叶猪,下同)的猪肉中含有肌苷酸等风味物质,显著提高猪肉胴体品质和猪肉风味。设计猪尿处理,采用红泥膜沼气池降解工程工艺应用后,降解水兑2倍清水对桑树喷灌工艺,减少人工处理劳力和成本费用。设计10%抗非洲猪瘟中药饲料添加剂+桑叶饲料,预期防治非洲猪瘟总效果85%以上。桑叶养猪实用技术可行,供同行参考。  相似文献   

野生稻种质在水稻抗病育种上的研究及应用进展     

王樟凤  盛文涛  柴学文  饶友生 《山地农业生物学报》2016,(6):61-67

野生稻是一个重要的基因资源库,包含了水稻各种病害的抗性基因。本文综述了野生稻丰富的抗性资源及其基因的挖掘,以及在水稻育种上的利用研究进展,并对目前存在的突出问题,提出了野生稻种质资源抗病育种的发展建议。  相似文献   

Pre-breeding for resistance to Verticillium wilt in olive: Fishing in the wild relative gene pool     

《Crop Protection》2015

This study aimed to identify new sources of resistance to Verticillium wilt in olive. We evaluated various types of genotypes: wild olive trees (Olea europaea subsp. europaea var. sylvestris), genotypes belonging to related subspecie (Olea europaea subsp. guanchica) and genotypes coming from crosses between Picual cultivar and wild olive trees. Fifty-six genotypes were inoculated by dipping roots and then screened under controlled conditions to test their resistance to a highly virulent Defoliating isolate of Verticillium dahliae. Picual (susceptible) and Frantoio (resistant) were control cultivars. Wide variability and significant differences were obtained in the evaluated disease parameters. The Relative Susceptibility Index (RSI), summarizing the disease parameters, was used for final classification of genotypes. Thirteen genotypes were categorized as resistant: eight wild olives from different locations, two genotypes belonging to subsp. guanchica populations from Canary Islands and three genotypes obtained from one of the crosses Picual x wild. The identification of high levels of resistance to V. dahliae among wild olive genotypes may be helpful for the management of this disease. The resistant genotypes could be used as rootstocks for susceptible olive cultivars or parents in future breeding programs.  相似文献   

First serological evidence of West Nile virus infection in wild birds in Northern Algeria     

《Comparative immunology, microbiology and infectious diseases》2020

While the epidemiology of Flaviviruses has been extensively studied in most of the Mediterranean basin, little is known about the current situation in Algeria. In order to detect the circulation of West Nile (WNV) and Usutu viruses (USUV) in Kabylia, 165 sera were collected from two wild birds species, namely the long distance migrant Turdus philomelos (song thrush) (n = 92) and the resident Passer domesticus (house sparrow) (n = 73). A total of 154 sera were first analyzed by commercial competition ELISA. WNV and USUV micro-neutralization tests were performed on all c-ELISA positive sera and all samples with poor volume. Overall, 7.8 % (CI95 %: 3.5–11.9) were positive by c-ELISA. Positive results were detected in 12.5 % (CI95 %:5.6–19.4) of song thrushes and 1.5 % (CI95 %: 0.0–4.5) for sparrow.Micro-neutralization tests revealed an overall seroprevalence of 6.7 % for WNV (CI95 %: 2.9–10.3), Neutralizing antibodies were found in 8.7 % (CI95 %: 3.0–14.4) for song thrushes and in 4.1 % (CI95 %: 0.0–8.7) of sparrows. The current study demonstrates significant seroprevalence of WNV antibodies in wild birds in Algeria.  相似文献