葡萄球菌性关节炎肉鸡免疫器官的病理学变化     

谷长勤  王德海  胡薛英  谢长清  程国富 《中国兽医学报》2007,27(6):903-906,909

利用鸡源致病性金黄色葡萄球菌复制鸡葡萄球菌性关节炎的病理模型,研究免疫器官的主要病理学变化及其内CD4 和CD8 T淋巴细胞的动态变化。结果表明:鸡接种细菌后呈典型的关节炎症状。脾脏肿大,表面呈网格状,法氏囊黏膜增厚,盲肠扁桃体和胸腺上散在出血点。光镜下脾脏淋巴小结早期数目增多,后期呈灶状坏死,法氏囊水肿,淋巴小结坏死液化。CD4 和CD8 T淋巴细胞的变化为:脾脏在接种后7 d淋巴小结内的CD4 和CD8 T淋巴细胞已明显多于对照组,与对照组比差异极显著(P<0.01);接种后7 d,法氏囊的淋巴滤泡周围的间隙中检出较多的阳性细胞,在接种后14 d CD4 T淋巴细胞达到高峰,随后下降,与对照组相比在接种后7 d差异显著(P<0.05),在接种后14 d差异极显著(P<0.01);而CD8 T淋巴细胞在接种后7 d就达到高峰,与对照组相比差异极显著(P<0.01);盲肠扁桃体的CD8 T淋巴细胞在接种后7 d上升,21 d达到最高值;胸腺组织中阳性细胞数对照组和试验组都很少,试验组的阳性细胞数的变化没有明显的规律。鸡接种金黄色葡萄球菌后免疫器官内CD4 和CD8 T淋巴细胞数目增多,表明T淋巴细胞参与了金黄色葡萄球菌引起鸡的关节炎的发生发展过程。  相似文献   

间接ELISA检测鸡病毒性关节炎抗体方法的建立及其应用     

娄高明  宣华 《中国兽医学报》1992,(3)

用蔗糖密度梯度离心法提纯鸡病毒性关节炎病毒(AVAV)作包被抗原,建立了间接ELISA的最佳工作程序,其中抗原包被浓度为0.61μg/孔,被检血清为1:50.酶结合物1:1600;其灵敏度为琼脂凝胶沉淀试验(AGP)的238.5倍.在受检的529份鸡血清中,该法检出率(85.1%)显著高于AGP(46.0%).间接ELISA对S1133疫苗及AVAV—J株接种鸡抗体消长情况的观察结果表明,S1133疫苗常量接种后所产生的免疫力足以抵抗强毒株AVAV—J的攻击,AVAV—J毒株的良好免疫原性.使其有可能成为研制国产疫苗的候选毒株.  相似文献   

鸡病毒性关节炎的研究概述     

任绪杰 《水禽世界》2007,(2):44-46

鸡病毒性关节炎(Avian Virus arthritis)也称“传染性腱鞘炎”,是近年来发现的一种由禽呼肠孤病毒引起的鸡慢性传染病,以侵害跗关节、趾关节及其肌腱和心肌为特征,该病分布广泛,在世界各地均有发生,对鸡特别是肉鸡的生产带来的经济损失很大。文章就AVA的病原学、诊断方法及免疫预防等作一概述。  相似文献   

Rosiglitazone inhibits osteoclastogenesis in rheumatoid arthritis by down-regulating RANKL expression and suppressing ERK phosphorylation     

WEI Xiu-ning  ZHENG Dong-hui  MO Ying-qian  MA Jian-da  DAI Lie 《园艺学报》2015,31(5):911-916

AIM: To investigate the effects of rosiglitazone on fibroblast-like synoviocyte (FLS)-induced osteoclastogenesis in rheumatoid arthritis (RA) and the related mechanism. METHODS: RA-FLS were cocultured with peripheral blood monocytes from healthy volunteers in the presence of macrophage colony-stimulating factor (M-CSF) and rosiglitazone. Osteoclasts were assayed by tartrate-resistant acid phosphatase (TRAP) staining. Resorption lacunae area was identified by toluidine blue staining and quantified by image analysis software. The mRNA expression of RANKL and OPG was evaluated by real-time PCR, and the protein levels of RANKL, OPG, p-ERK, p-p38 and p-JNK were measured by Western blot. RESULTS: Compared with control group (without rosiglitazone treatment), rosiglitazone at concentration of 15 μmol/L significantly decreased the number of osteoclasts (P<0.01) and resorption lacunae area (P<0.05). The expression of RANKL at mRNA and protein levels was significantly down-regulated by rosiglitazone at concentration of 15 μmol/L, while the mRNA and protein expression of OPG was up-regulated (P<0.01). Rosiglitazone (15 μmol/L) significantly decreased the protein level of p-ERK (P<0.05), but not the protein level of p-p38 or p-JNK (P>0.05). CONCLUSION: Rosiglitazone inhibits RA-FLS-induced osteoclast formation and its resorption activity by down-regulating RANKL expression and ERK phosphorylation, suggesting that rosiglitazone may inhibit RA osteoclastogenesis and bone resorption.  相似文献   

Isolation and Identification of the Arthritis Pathogen from Dairy Cow     

JIAN Zong-hui  DUAN Rui-rui  CHEN Pei-fu 《中国畜牧兽医》2016,43(2):542-547

This study was aimed to avoid the dairy cow arthritis,which could caus huge economic losses to cattle farms.Joint effusion was collected from diseased dairy cows,and the pathogens was isolated and cultured using tryptone broth agar followed by morphological,biochemical tests and PCR amplifying 16S rRNA gene fragments and TA cloning and sequencing to identify it.It was found that the bacteria could form beta hemolytic ring on blood AGAR;And it appeared a single individual or short catenation gram-positive by optical microscopy;The results of biochemical identification showed that esculin sesquihydrate,maltose,sucrose,glucose,and hydrogen peroxide enzyme tests all presented positive;It was sensitive to cefazolin,enrofloxacin,ciprofloxacin,ampicillin,streptomycin,chloramphenicol,neomycin and cefepime,while was resistant to tetracycline,erythromycin,doxycycline and amikacin.The nucleic acid homology of this strain with Staphylococcus aureus was 91%,and with the Staphylococcus carnosus was 92%.According to the 16S rRNA gene fragment sequencing results,and the biological characteristics analysis,the initial isolates were identified as similar to Staphylococcus spp bacteria.The results of this study could provide necessary experimental basis for future diagnosis,prevention as well as treatment of arthritis in cattle farm.  相似文献   

Caprine mycoplasmosis by M. capricolum (author's transl)     

P Perreau  A Breard 《Comparative immunology, microbiology and infectious diseases》1979,2(1):87-97

Identified in France in 1972, this severe goat disease is now well established in goat breeding areas.Natural infection occurs as a contagious agalactia syndrome, the local lesions of which appear after a septicema stage which can lead to the death of animals in poor condition.This fatal evolution is the general rule among kids which die within a few days, after being fed with infectious milk.The experimental disease is easy to reproduce with wild strains of M. capricolum; it allows to study the immune response and also to assess the value of therapeutic agents.The strains isolated in France constitute a very homogeneous group, from a microbiological point of view; compared with reference strains, some serological variations are demonstrable, which can raise difficulties in strain identification.Microbiological diagnosis is fairly easy, but serological diagnosis is not yet standardized and a manifest cross-reaction exists with another pathogenic agent for goats, M. mycoides var. mycoides.Up to now, the epidemiological characteristics of this mycoplasmosis are to a great extent unknown; recovered animals are carriers which allows the disease to be maintained in flocks, but the features of this carrier state (frequency, duration, predisposing factors) are not well known. Screening of such goats remains uncertain.Specific treatment must be carried out with some antibiotics; indications and results are to be discussed.  相似文献   

鸡病毒性关节炎病毒（AVAV）的分离与鉴定     

唐雨德  周宗安 《畜牧与兽医》1994,26(4):152-153

用ＳＰＦ鸡胚细胞培养，从临床上呈现类似鸡病毒性关节炎症状病鸡的趾屈肌组织中分离出一株病毒，电镜观察，病毒直径约为７５ｎｍ，无囊膜，呈六边形，双层衣壳，理化检查，病毒对５－氟脱氧尿核苷、ｐＨ５．０和乙醚耐受，胰蛋白酶对病毒感染价无影响；核酸电泳分析，病毒由１０个核酸片段组成，呈３－３－１－３分布；血清学试验，该病毒与ＡＶＡＶＳ＿（１１３３）株呈交叉反应。上述检查结果表明分离的病毒为鸡病毒性关节炎病毒，结合现场病鸡的临床症状和双份血清抗体检测结果，认定该鸡场此次发生的传染病为鸡病毒性关节炎。  相似文献   

鸡病毒性关节炎弱毒疫苗的研制Ⅰ．弱毒株的筛选及其对雏鸡的安全性     

唐雨德  周宗安  翟春生  王元伦  顾志香 《中国兽医学报》1994,(2)

将鸡病毒性关节炎病毒（ＡＶＡＶ）鸡胚毒Ｊ─１株适应于鸡胚细胞和Ｖｅｒｏ细胞，经连续传代，通过４０．５℃、３７℃、３２℃的交替升降培养温度培养，筛选出１株毒力较弱的克隆株，该克隆株蚀斑大小为２．７～３．１ｍｍ。以１０５．７２５ＴＣＩＤ５０注射于１日龄敏感雏鸡的足掌皮下和以１０４．７２５～１０６．７２５ＴＣＩＤ５０注射于１日龄敏感雏鸡胸部肌肉内，对雏鸡无致病性。该克隆株在敏感雏鸡内连传３代，无毒力返强现象。这一结果表明，本试验筛选出的ＡＶＡＶ弱毒株对雏鸡具有良好的安全性和遗传稳定性。  相似文献   

Analysis of factors influencing prognosis in foals with septic arthritis     

Vos NJ  Ducharme NG 《Irish veterinary journal》2008,61(2):102-106

The purpose of this paper was to identify factors that would positively or negatively affect the short-term survival rate of foals with septic arthritis. Medical records of 81 foals (≤seven months of age) with a clinical diagnosis of septic arthritis, referred to the equine hospital at Cornell University Hospital for Animals, between 1994 and 2003 were reviewed. Signalment, age at presentation, number of affected joints, joint fluid parameters, bacterial agents, treatment modalities and year of treatment were compared between survivors and nonsurvivors. Sixty-two of 81 foals (77%) were discharged from the hospital and classified as 'survivors'. Multiple joint involvement and detection of intra-articular Gram-negative, mixed bacterial infection and degenerate neutrophils were negatively associated with short-term survival. Initiation of treatment within 24 hrs of onset of clinical signs and combination of treatment modalities were positively correlated with survival. Further investigation is needed to determine if these two factors have a similar influence on athletic performance.  相似文献   

Effects of IL-22 on rheumatoid arthritis fibroblast-like synoviocytes     

LIU Meng  LIU Yan  YANG Meng-ru  MO Bi-yao  PAN Yun-feng 《园艺学报》2016,32(7):1273-1278

AIM: To determine the effects and mechanisms of interleukin-22(IL-22) on the fibroblast-like synoviocytes(FLSs) from rheumatoid arthritis(RA) patients. METHODS: RA-FLSs were cultured by tissue culture method. RA-FLSs were incubated with different concentrations of IL-22(0,1,10,100 μg/L) for 24 h, 48 h and 72 h. The cell viability was examined by CCK-8 assay. IL-22 at concentration of 10 μg/L was used to stimulate RA-FLSs for 24 h, and the change of cell cycle distribution was identified by flow cytometry. The effects of IL-22 at concentrations of 0, 1, 10, 100 μg/L and/or STA-21(a STAT3 inhibitor at concentrations of 0, 25, 50 μmol/L) on the protein levels of Bcl-2 and p-STAT3 in the RA-FLSs were determined by Western blot.RESULTS: Compared with control group, stimulation of rhIL-22 at different concentrations for 24 h, 48 h and 72 h, the cells viabilityof RA-FLSs were obviously increased(P<0.05). After co-cultured with 10 μg/L rhIL-22 for 24 h, the percentages of RA-FLSs were obviously increased in the G₂/M+S phase and decreased in the G₀/G₁ phase. At the same time, rhIL-22 increased, but STA-21 decreased the protein levels of Bcl-2 but p-STAT3 in the RA-FLSs obviously(P<0.05). Treatment with STAT3 inhibitor STA-21 reversed the effect of IL-22-induced Bcl-2 upregulation in the RA-FLSs(P<0.01). CONCLUSION: STAT3 is critical in the process of IL-22-induced Bcl-2 upregulation in RA-FLSs, indicating that IL-22 may play a role in the apoptosis of RA-FLSs.  相似文献