注射含HCG和CPE的W／O／W复乳诱导日本鳗鲡性腺发育成熟的研究   总被引：4，自引：1，他引：4       下载免费PDF全文

邓岳松  林浩然  谢骏  潘德博  王广军  许梓荣 《中国水产科学》2001,8(2):44-47

用含HCG及鲤脑垂体（PG）匀浆液提取物（CPE）的W／O／W复乳，对塘养雌鳗进行注射催熟实验，雌鳗平均GSI达35．18％，最高达56．5％，其催熟效果较常规注射方法（平均GSI 27．9％，最高50．4％）好，用含LHRH－A，HCG，CPE的复乳和DOM生理盐水混悬剂对部分达到性成熟的鳗鲡进行催产，用W／O／W复乳诱导成熟的鳗鲡，排卵率为75％，用常规方法催熟的鳗鲡排卵率为55％，注射复乳制剂后，鳗鲡血清GtH含量可较长时间的保持较高水平，血清GtH的变化较常规注射方法缓和，注射乳剂还可使鳗鲡脑垂体GtH含量升高。  相似文献   

大弹涂鱼血细胞发生的研究   总被引：14，自引：0，他引：14       下载免费PDF全文

李长玲  曹伏君  刘楚吾  师尚丽 《中国水产科学》2001,8(1):10-14

通过对大鲜涂鱼血液、肾脏、脾脏组织涂片的观察，发现其肾脏、脾脏内白细胞的发育大致经过了3个阶段，即原始阶段、幼稚阶段、成熟阶段。造血母细胞从造血器官释放入外周血中有一成熟过程。着重描述了大弹涂鱼的红细胞、嗜中性粒细胞、淋巴细胞和单核细胞发生过程中各阶段细胞的形态特征。  相似文献   

长吻鮠胚胎和胚后发育与温度的关系   总被引：8，自引：0，他引：8  

张耀光  何学福  蒲德永 《水产学报》1991,15(2):172-176

鱼类的胚胎发育(embryonic development)与水温具有十分密切的关系。国内外一些学者先后探讨了鲤鱼(Cyprinus caripo)、湖红点鲑(Salvelimus namaycush)等多种鱼类发育的有效温度、最适温度和临界温度,以及鱼类胚胎对水温的敏感性、依从性等内容。  相似文献   

盐度对黄鲷胚胎发育及早期仔鱼生长的影响   总被引：16，自引：0，他引：16  

施兆鸿 《水产学报》2004,28(5):599-602

The effect of salinity on embryo and early larva development of Dentex tumiforns was discussed. Floating forms of egg in different salinities, optimal salinity for embryonic development and early larva growth was studied. The results showed: 1. In immobile condition, all eggs sank at salinity of 32.0, most of egg ssuspended in the middle of water at 34.0, and all eggs floated on the surface when salinity above 36.0. 2. Eggs did not hatch out at salinity of below 10.0 or above 60.0 and the death time of egg gradually moved up with increase or decline of salinity. Eggs hatched out at salinity range of 15.0 and 50.0, including abnormal larva. There was indistinct difference in speed of embryonic development (about 36-40h) within the salinity of 15.0 and 50.0. However the salinity had a great effect on larval survival after hatching and abnormal rate. The relation between hatching rate and salinity variation showed parabola but abnormal rate showed inverted parabola. The hatching rate was 81%-86% and abnormal rate of yolk sac larva was 27%-30% in suitable salinities of 27.0-39.0. The hatching rate was 89%-91% and abnormal rate was 13%-16% in optimal salinities of 33.0-36.0. The oil ball of abnormal larva was located in the central or front position. With increased extent of salinity, spondyle of larva bended and number of larva with arrhythmia increased. 3. The optimal salinities were 30.0-35.0 based on SAI. The test with SAI showed that SAI of early larvae was 41.25-47.53 at salinities of 30.0-35.0, the yolk and oil ball of 7-8 days larva was completely absorbed with a survival rate of 88%.  相似文献   

Impaired larval development in broods of eelpout (<Emphasis Type="Italic">Zoarces viviparus</Emphasis>) in Danish coastal waters     

J.?StrandEmail author  L.?Andersen  I.?Dahll?f  B.?Korsgaard 《Fish physiology and biochemistry》2004,30(1):37-46

Eelpouts (Zoarces viviparus), a viviparous fish, were sampled in Danish coastal waters during October and November 2001 and 2002, in ten different areas, which are receiving effluents from cities and industry to more or minor degree. The presence of gross abnormalities in eelpout broods has been suggested to be a useful biomarker of the impact of hazardous substances on fish reproduction in the marine environment as chronic exposure to various substances has the potential to induce severe developmental defects in fish embryos and larvae. Relatively high frequencies of female eelpouts (20–53) with elevated levels (>5) of larvae with developmental defects in the broods were found in four shallow fjords with effluents from larger cities and industry compared to areas. Deformations like spiral or bend shapes of the spinal axis, cranio-facial defects, eye lesions or loss of eyes were the dominating types. In some ofthe areas with the highest incidences of developmental defects, the adults had significantly enlarged livers, which may also be a possible effect caused by contaminant exposure. In two of the areas, relatively high frequencies of the broods contained larvae, which had died late but without any visible developmental defects. These two areas were affected by severe oxygen depletion prior to sampling time suggesting that examination of broods in the eelpout may include not only impact of hazardous substances but also effect of eutrophication-related problems on fish reproduction in the marine environment.  相似文献   

Three different levels of dietary vitamin D₃ fed to first-feeding fry of Atlantic salmon (Salmo salar L.): effect on growth, mortality, calcium content and bone formation     

I.E. GRAFF  S. HØIE  G.K. TOTLAND  & Ø. LIE 《Aquaculture Nutrition》2002,8(2):103-111

Atlantic salmon ( Salmo salar L.) fry were reared on a fishmeal-based diet with three different levels of vitamin D₃ (0.2, 5 and 57 mg vitamin D₃ kg^–1 feed, ww) from first-feeding for 14 weeks. No significant differences were recorded in weight, length, specific growth rate, mortality, or kidney calcium concentration between the different dietary groups. No skeletal malformations or histopathological changes were recorded in any of the dietary groups. These results suggest Atlantic salmon fry to be highly tolerant of megadoses of vitamin D₃ over a limited period of time.  相似文献   

鱼类早期发育阶段摄食行为研究现状   总被引：2，自引：0，他引：2  

柳琪  区又君 《南方水产》2006,2(1):71-75

对鱼类早期阶段的摄食行为研究进行了综述。重点阐述了胃含物分析法、饥饿对摄食的影响、摄食器官的形态发育特征与摄食行为的关系、食物选择及其生理生态因子对摄食的作用。初步探讨了仔稚鱼饥饿后的捕食量和捕食速度的变化,并结合食物保障,分析了食物选择性指数I在鱼类摄食行为研究中的应用。  相似文献   

斜带石斑鱼胚胎及仔稚幼鱼形态发育   总被引：14，自引：4，他引：14       下载免费PDF全文

张海发  刘晓春  刘付永忠  王云新  林蠡  黄国光  舒琥  罗国武  林浩然 《中国水产科学》2006,13(5):689-699

对斜带石斑鱼(Epinephelus coioides)的胚胎及仔稚幼鱼形态发育进行了观察与研究,详细描述从受精卵到初孵仔鱼的28个具体发育时期的形态特征和发育时间。根据研究观察结果,将斜带石斑鱼胚胎发育划分为卵裂期、囊胚期、原肠胚期、神经胚期和器官形成期。在水温(25±0.5)℃、盐度31.0p、H 7.8的海水中,斜带石斑鱼胚胎历时28 h 30 min完成整个胚胎发育孵化出膜。胚后发育主要根据卵黄囊、腹鳍棘及第二背鳍棘、鳞片、体色的变化分为仔鱼期、稚鱼期、幼鱼期。仔鱼期根据卵黄囊的有无又分为早期仔鱼和晚期仔鱼。在水温24.5~29.2℃,盐度28.8~33.5,pH 7.5~8.5的海水中,培育至36 d,发育最快的斜带石斑鱼结束仔鱼期进入稚鱼期;培育至42 d,发育最快的稚鱼完成变态,成为幼鱼。斜带石斑鱼胚后发育过程中最明显的变化是腹鳍棘与第二背鳍棘以及鳍棘上小刺的长出与收回。  相似文献   

我国水产养殖保险发展的几点建议     

王莉  骆乐 《渔业经济研究》2006,(1):32-35

水产养殖业是风险较大的产业,水产养殖者在水产养殖的过程中,难免遭受自然灾害和意外事故,保险机构如能对水产养殖者所遭受的经济损失及时提供经济保障,必将为水产养殖业的稳定发展起到重要作用。本文对中国水产养殖保险发展现状、必要性及存在的问题等进行了阐述,并提出一些建议。  相似文献   

盐度对真鲷受精卵发育及仔稚鱼生长的影响   总被引：28，自引：1，他引：28       下载免费PDF全文

王涵生 《中国水产科学》2002,9(1):33-37

在室内水池以溢水法收集真鲷 (Pagrosomusmajor)受精卵 ,并置于不同盐度海水中静水孵化 ,孵出仔鱼以流水培育。设置盐度梯度为 17.0、2 2 .0、2 7.0及自然盐度 3 3 .0 ,水温分别为 19.5～ 2 1.5℃ (孵化 )、2 0 .5～ 2 3 .5℃(培育 )。结果表明∶①真鲷受精卵在自然盐度海水中发育很好 ,孵化率较高 ( 85 .0 %～ 97.0 % ) ,畸形率较低 ( 3 .2 %～ 2 3 .7% ) ;在低盐度条件下静水孵化 ,发育不好 ,孵化率为 4 8.0 %～ 1.0 % ,且盐度越低 ,孵化率也越低。充气孵化时 ,盐度低至 17.0 ,孵化率有时也可达 97.1% ,但畸形率高达 85 .7%～ 10 0 %。②孵化仔鱼中有 2类畸形 ,第 1类畸形表现为身体或尾部弯曲 ,油球位于卵黄囊中部或前部 ,油球数多于 1个 ,盐度越低 ,畸形率越高 ;第 2类畸形表现为仔鱼膜膨大、围心腔扩大 ,这类畸形只出现于低盐度条件下 ,盐度越低 ,畸形率越高 ,且往往与第 1类畸形症状并发。③不同盐度对真鲷仔稚鱼的生长速度没有显著影响 ,各盐度条件仔稚鱼经 3 1d的培养 ,其平均全长为 19.6～ 2 0 .7mm( P >0 .0 5 ) ,没有显著的差别。④海水盐度越低 ,仔稚鱼的存活率越高 ,各组的平均存活率为 ,盐度17.0时 ,3 6.3 % ;盐度 2 2 .0时 ,3 6.2 % ;盐度 2 7.0时 ,2 7.1% ;盐度 3 3 .0时 ,16.3 %。  相似文献