One-step RT-PCR for the Detection of Infectious Bursal Disease Virus in Clinical Samples     

Kataria RS  Tiwari AK  Nanthakumar T  Goswami PP 《Veterinary research communications》2001,25(5):429-436

A single-tube, non-interrupted, one-step RT-PCR has been standardized to amplify the hypervariable region of the VP2 gene sequence of infectious bursal disease virus (IBDV). The technique standardized on purified viral RNA was successfully applied to the detection of the virus directly in clinical samples. The amplified products were confirmed to be IBDV specific by their size in ethidium bromide-stained agarose gel, nested PCR and restriction enzyme digestion. Digestion of the amplicons with StyI restriction enzyme also differentiated classical virus from six very virulent field isolates. The sensitivity of the one-step RT-PCR was found to be 0.2 pg of viral RNA.  相似文献   

硼中毒对固始鸡空肠发育的影响   总被引：11，自引：1，他引：11  

王珏  顾有方  李升和  商常发  陈会良 《中国兽医学报》2005,25(5):514-517

120只1日龄固始鸡随机分为2组，分别饮用添加硼0、400mg／L的水，研究了硼中毒对固始鸡空肠发育的影响。试验期为6周，每周定时每组取鸡10只，颈静脉放血致死，取空肠，Bouin’s液固定，制作石蜡切片，HE染色，显微镜观测与拍照。结果：（1）试验组空肠绒毛长度极显著低于同周龄对照组（P〈0．01）；肠腺长度第1周龄极显著低于对照组（P〈0．01），第2周龄显著低于对照组（P〈0．05）。（2）试验组1～4周龄空肠出现上皮细胞肿胀、脱落，绒毛中抽结缔组织水肿、毛细血管扩张，肠腺囊肿等痛变。试验表明，硼中毒对固始鸡小肠绒毛及肠腺发育有明显抑制和毒害作用，对机体消化吸收功能产生严重影响。  相似文献   

胸腺素α_1对墟岗黄鸡免疫器官组织学结构的影响   总被引：4，自引：0，他引：4  

陈芳  计慧琴  卢玉葵  王丙云  江青艳 《中国畜牧杂志》2005,41(7):16-18

本试验研究了不同剂量的胸腺素α1(Tα1)对墟岗黄母鸡免疫器官指数和组织学结构的影响。结果表明:0.015mg/kg体重剂量的Tα1可显著提高鸡免疫器官指数,可使胸腺皮质增宽,髓质胸腺小体数和胸腺细胞数增加;脾脏胸腺依赖区和法氏囊依赖区细胞增殖;高剂量Tα1则引起法氏囊髓质局部细胞变性和坏死。  相似文献   

五种市售鸡传染性鼻炎灭活疫苗免疫效力比较     

孙惠玲  苗得园  陈晓峰  张培君  龚玉梅  唐桂运  Anne-Lise  Saint-Ger 《中国预防兽医学报》2005,27(6):544-548

本研究对国内市场上几个主要鸡传染性鼻炎灭活疫苗生产厂家生产的灭活疫苗免疫鸡只进行血清HI抗体水平测定和攻毒,比较不同公司所产疫苗效力的效力差异,并评估A、C型二价灭活疫苗两次免疫鸡群对国内B型分离株:DL-1株和最近从免疫失败鸡场分离到的SD-1株的交叉保护作用,分析免疫失败的原因.结果表明:A、D、E公司的疫苗免疫两次后,A型HI抗体阳性率分别为92.5%、100%和95%,滴度分别为33.9、55.1和59.9,攻毒保护率都是100%.C型HI抗体阳性率分别为72.5%、38.5%和77.5%,滴度分别为11.4、2.7和27,攻毒保护率分别为80%、70%和80%.而B和C公司的疫苗免疫两次后A型HI抗体阳性率分别为59.4%、77.1%,抗体滴度分别为5.4和21.8,攻毒保护率分别为50%和66.7%;C型HI抗体阳性率分别为54.1%和51.4%,抗体滴度分别为6.1和6.8,攻毒保护率分别为38.3%和50%.在五个疫苗产品中,以A、D、E的保护效力较好,B、C产品效力较差.另外,A、C型二价灭活疫苗免疫后不能对B型菌的攻击提供保护,其A、C型HI抗体阳性率、抗体滴度与对B型菌攻毒保护率无相关性.  相似文献   

肾型传染性支气管炎病毒对鸡嘌呤代谢的影响研究   总被引：1，自引：0，他引：1  

马跃  田晋红  张晓瑜  李小姝  刘爱国  张祖梅  冉毅 《中兽医医药杂志》2005,24(6):21-23

用T株肾型传染性支气管炎病毒(IBV)人工感染14日龄非免疫土种鸡建立感染模型,于攻毒前、攻毒后48 h、72 h、96 h、120 h 采集血样,测定血浆黄嘌呤氧化酶(xanthine oxidase, XOD)活性,探索鸡嘌呤代谢变化与肾型IBV致病机理之间的关系.结果表明,与攻毒前相比较, 攻毒后48 h和72 h血浆XOD活性明显增强, 差异显著(P<0.01,P<0.05).结果提示,XOD活性升高引起的嘌呤代谢紊乱可能是鸡肾型IB的病理机制之一.  相似文献   

NDV和IBV感染对肉鸡大肠杆菌病发病的影响     

王晶钰  张万珍  张彦明  郭抗抗 《动物医学进展》2003,24(2):92-93

为了解新城疫弱毒苗和传染性支气管炎弱毒苗接种对肉鸡大肠杆菌病发病的影响。对8日龄肉仔鸡用不同剂量NDVclone-30株或IBV-H120株进行滴鼻及饮水,48h后相同途径接种2株不同“O”血清型的致病性大肠杆菌。结果表明,致病性大肠杆菌单独接种鸡死亡率在20%-25%之间,而NDVclone-30株或IBVH120株与致病性大肠杆菌联合接种鸡死亡率有明显增加达40%-75%。  相似文献   

Effects of High-tannin Sorghum and Bentonite on the Performance of Laying Hens     

Ambula MK  Oduho GW  Tuitoek JK 《Tropical animal health and production》2003,35(3):285-292

The effects of feeding high-tannin sorghum (HTS) (5% catechin equivalents (CE)) on the performance of laying hens were examined in two experiments. In experiment 1, the effects of including varying levels of HTS in layer diets were investigated. Sixty-three Isabrown hens were randomly assigned to each of the seven diets over a period of 56 days. In experiment 2, the effects of bentonite on the utilization of HTS (3.74% CE) by laying hens were investigated. Forty-five Isabrown hens were randomly assigned to each of five diets over a period of 42 days. In both experiments, weekly measurements were made for feed intake (FI), percent hen-house production (% HHP), egg weight and egg specific gravity (ESG). The results of experiment 1 showed that the different sorghum inclusion levels had no effect (p>0.05) on HHP, egg weight and ESG, while those of experiment 2 showed that maize and LTS diets gave similar weight gain, FI and FE (p>0.05). However, HHP was higher (p<0.05) for the LTS diet than for the other diets. The addition of bentonite of 0.25% or 0.5% to the HTS diet did not improve (p>0.05) layer performance. When all the results were considered together, it was concluded that HTS can be used in layer diets without adverse effects on performance. The use of bentonite to deactivate sorghum tannins is not considered necessary.  相似文献   

Growth and Apparent Absorption of Minerals in Broiler Chicks Fed Diets with Low or High Tannin Contents   总被引：5，自引：1，他引：4  

Hassan IA  Elzubeir EA  El Tinay AH 《Tropical animal health and production》2003,35(2):189-196

Sorghum grains with low (0.28 per cent catechin equivalent (%CE) or high (1.36 %CE)) tannin contents were used to study the effect of tannin on growth and on the apparent absorption of Ca, P, Mg, Na, K, Fe and Co by chickens. High-tannin sorghum caused a highly significant (p0.01) reduction in the weight gain and feed intake of broiler chicks compared to low-tannin sorghum and increased the feed conversion ratio (p0.01). The amount of tannin did not affect mortality. The apparent absorption of Ca, P, Mg, Na, K, Fe and Co was reduced (p0.01) by feeding sorghum grains with a high tannin content.  相似文献   

The Value of Tissue Imprint Hybridization for Rapid Detection of Infectious Bursal Disease Virus from Field Outbreaks     

Parthiban M  Thiagarajan V 《Tropical animal health and production》2002,34(5):391-398

The use of a peroxidase labelled PCR generated probe followed by enhanced chemiluminescence hybridization assay detected infectious bursal disease virus directly from bursal imprints on a nylon membrane. Tissue imprint hybridization proved to be a simple, rapid and safe means of detecting IBD virus for screening large numbers of field samples. The PCR generated probe was highly specific for IBD virus and did not hybridize with cellular nucleic acids in control imprints. Tissue imprint hybridization was found to be a more sensitive method than conventional antigen detection assays.  相似文献   

Efficacy of Sarafloxacin in Broilers after Experimental Infection with Escherichia coli     

Chansiripornchai N  Sasipreeyajan J 《Veterinary research communications》2002,26(4):255-262

Infections of chickens with Escherichia coli serotypeO78 can be treated with the antibiotic sarafloxacin. Three experiments were conducted on the administration of this drug to chickens that had been experimentally infected with E. coli. The birds were monitored for 10 days after infection for their average daily gain (ADG) and feed conversion ratio (FCR), and the post-mortem pathology was assessed. In the first experiment, sarafloxacin (20 mg/L, equivalent to 5 mg/kg live weight per day), given in the drinking water for 3 days after infection, led to a reduction in the mortality from 75% to 27%, but the ADG of the treated birds was still less than that of the uninfected controls. In the second experiment, when the sarafloxacin was administered at the same dose in the water but over only 2 h, there was also a considerable reduction in mortality, and the ADG and the FCR also improved significantly. In the third experiment, the dose dependence of the drug was tested. The birds were given 5 and 10 mg/kg per day sarafloxacin in each group, starting within 2 h after infection. This rapid administration of the drug completely prevented mortality, while the ADG and FCR were similar to those of the uninfected controls.  相似文献