楚雄华山松种子园无性系幼苗叶枯病病原菌鉴定     

吕则佳  陈健鑫  冯峻  朱艳  董云祥  罗正平  马焕成  伍建榕   《西北林学院学报》2022,37(4):181-187

为明确楚雄市华山松种子园无性系幼苗叶枯病的病原,采用传统形态学和分子生物学相结合的方法对华山松叶枯病进行病原鉴定。结果表明,引起华山松叶枯病的病原菌为胡枝子拟盘多毛孢（Pestalotiopsis lespedezae）和互隔链格孢（Alternaria alternata）。  相似文献   

华北落叶松优良无性系选育研究     

李改萍 《防护林科技》2021,(2):26-28

为筛选出华北落叶松(Larix principis-rupprechtii)优良无性系,对大同市长城山林场华北落叶松国家重点林木良种基地的40个华北落叶松无性系树高、胸径生长量指标进行了调查,结果表明:7、7509、113这3个华北落叶松无性系长势最好,是华北落叶松优良无性系的重点选择对象;而123、713、124、204、94、7511、67这7个华北落叶松无性系长势最差,可作为种子园遗传疏伐的对象。研究结果为华北落叶松种子园后期高世代育种提供理论依据。  相似文献   

大豆转录因子GmDof1.5的克隆与非生物胁迫诱导表达     

何佳琦  翟莹  张军  邱爽  李铭杨  赵艳  张梅娟  马天意 《浙江农业学报》2021,33(1):1

Dof转录因子在植物的生长发育和非生物胁迫响应中起重要的调控作用。利用RT-PCR技术从大豆中克隆了一个功能未知的Dof转录因子基因GmDof1.5,该基因位于大豆基因组15号染色体上,ORF全长540 bp,编码含有179个氨基酸的蛋白质,相对分子质量为20.15 ku,等电点为9.82。蛋白序列预测发现,GmDof1.5蛋白含有一个典型Zf-Dof结合域,且含有大量磷酸化位点。蛋白系统进化分析表明,大豆GmDof1.5与豆科植物鸡血藤SsDof4的亲缘关系最近。实时荧光定量PCR结果显示,ABA、干旱、高盐、高温和低温胁迫均可不同程度地诱导GmDof1.5的表达,且GmDof1.5对高温胁迫的响应最为显著。  相似文献   

水肥胁迫对不同尾叶桉无性系叶绿素荧光特性的影响     

杨会肖  廖焕琴  杨晓慧  徐放  张卫华  潘文 《广东农业科学》2021,48(5):92-101

【目的】了解水分和养分胁迫对尾叶桉无性系叶绿素荧光特性的影响。【方法】以19个尾叶桉无性系为材料,采用2因素(水分、养分)3水平完全随机区组设计,对水肥胁迫6个月后尾叶桉苗木生长、叶形和叶绿素荧光特性进行研究。【结果】19个尾叶桉无性系间叶绿素荧光参数F_o、F_m、Φ_(PSⅡ)、qP、NPQ、ETR和F_v/F_m均有显著差异,说明不同无性系在不同水分和养分处理下具有不同的光能吸收和利用能力。在不同水肥胁迫处理6个月后,尾叶桉无性系苗木的树高、地径、冠幅分别与F_m、F_v/F_m、Φ_(PSⅡ)、ETR呈显著正相关,叶周长、叶面积与F_o呈显著负相关,同种类型性状间的相关系数较大(最高0.99),而不同类型间的相关性虽达到显著水平但相关值较小(最高为0.21)。26个性状可以综合为4个主成分,前4个主成分累计贡献率达93%,表明这些性状具有较强的代表性;根据最短距离和欧式距离聚类法将19个尾叶桉无性系划分为4组。【结论】尾叶桉无性系5(LDUA10)和10(LDUA24)生长表现好且具有较强的光化学特性,可作为优良无性系在生产上推广应用。  相似文献   

抑制消减杂交（SSH）及其在鱼类基因克隆中的应用     

程起群 《水产学报》2004,28(3):323-328

和其它脊椎动物一样,在鱼类的生命进程中,某种特定类型的细胞中只有比例约为10%～15%的基因表达.由于这些基因的特异性决定了从受精卵到机体死亡的整个生命过程,所以这些基因的时空表达是受到严格调控的.在不同发育阶段,不同生理状态和不同类型的细胞中,表达的基因各不相同.因此,比较两种不同类型细胞中基因表达的差异,可以探究表型的遗传原因,了解生命过程的基本信息,从而找到与发育、疾病等相关的基因.  相似文献   

Growth,Reproductive Performance,Carcass Characteristics and Meat Quality in F1 and F2 Progenies of Somatic Cell-Cloned Pigs     

Noritaka ADACHI  Daisuke YAMAGUCHI  Akiyuki WATANABE  Narumi MIURA  Seiji SUNAGA  Hitoshi OISHI  Michiko HASHIMOTO  Takatsugu OISHI  Masaki IWAMOTO  Hirofumi HANADA  Masanori KUBO  Akira ONISHI 《The Journal of reproduction and development》2014,60(2):100-105

The objective of this study was to examine the health and meat production of cloned sows and their progenies in order to demonstrate the application of somatic cell cloning to the pig industry. This study compared the growth, reproductive performance, carcass characteristics and meat quality of Landrace cloned sows, F1 progenies and F2 progenies. We measured their body weight, growth rate and feed conversion and performed a pathological analysis of their anatomy to detect abnormalities. Three of the five cloned pigs were used for a growth test. Cloned pigs grew normally and had characteristics similar to those of the control purebred Landrace pigs. Two cloned gilts were bred with a Landrace boar and used for a progeny test. F1 progenies had characteristics similar to those of the controls. Two of the F1 progeny gilts were bred with a Duroc or Large White boar and used for the progeny test. F2 progenies grew normally. There were no biological differences in growth, carcass characteristics and amino acid composition among cloned sows, F1 progenies, F2 progenies and conventional pigs. The cloned sows and F1 progenies showed normal reproductive performance. No specific abnormalities were observed by pathological analysis, with the exception of periarteritis in the F1 progenies. All pigs had a normal karyotype. These results demonstrate that cloned female pigs and their progenies have similar growth, reproductive performance and carcass quality characteristics and that somatic cell cloning could be a useful technique for conserving superior pig breeds in conventional meat production.  相似文献   

Cloning and sequence analysis of nine novel MYB genes in Taxodiaceae plants     

Yong-quan LU  Qing JIA  Zai-kang TONG 《林业研究》2014,25(4):795-804

Myeloblastosis(MYB) is one of the largest transcribed factor families in plants. To gain an overall picture of the evolution of MYB genes in relict plants, we cloned nine novel MYB genes in Taxodiaceae plants(Taxodium distichum, Taxodium ascendens, Cryptomeria japonica var. Sinensis, Cryptomeria japonica cv. Araucarioides, Cryptomer Japonica, Metasequoia glyptostroboides, Cunninghamia lanceolata, Taiwania cryptomerioides and Glyptostrobus pensilis). The deduced amino acid sequences for MYBs showed that the nine MYB proteins contained two DNA binding domains. The first domain is from amino acid position 29 to 78, wherein three tryptophanes at 33, 53 and 73 were separated by 19 amino acids, respectively. The second domain is from amino acid position 82 to 127, wherein three tryptophanes at 86, 105 and 124 were separated by 18 amino acids, respectively, whereas the first tryptophane at amino acid position 86 is replaced by a phenylalanine. The characterization of these conserved domains at nine MYBs indicated that they all belong to the R2R3-MYB group. The secondary structure analysis showed that α-helix and β-turn are the major motifs of the predicted secondary structure of MYBs. The three dimensional model of each MYB protein showed that the structure is like clip, making it more flexible and mobile. The similarities between the nine MYB proteins in Taxodiaceae were calculated. The highest identical value of 99% is between CjsMYB, CjMYB and CjaMYB, whereas the lowest value of 82% is between TaMYB and ClMYB. According to the phylogenetic tree, the distances between different genera were relatively large whereas those within genera were relatively small. As expected, accessions of the same genus formed a subgroup before being grouped with other genera.  相似文献   

玉米地方品种Glb1基因克隆及其遗传多样性分析     

陈发波  方平  姚启伦  程远芳  聂术君  秦林林 《广西农业科学》2014,45(8):1347-1352

【目的】了解我国西南地区不同玉米地方品种的遗传多样性,为合理利用这些玉米种质资源提供参考。【方法】以西南地区12份玉米地方品种为材料,对Glb1基因片段进行克隆、测序及序列比对,分析其遗传多样性。【结果】12份玉米材料Glb1基因序列长度为1138-1149 bp,其序列一致性为96.21%。12份材料Glb1基因序列间存在较多的变异位点,大于等于3 bp的插入/缺失有8个,主要位于第81-84、122-128、161-164、368-371、428-442、668-680、966-976和1003-1011 bp。不同玉米地方品种材料间的遗传相似系数为96.50%-100.00%,平均为98.58%。根据聚类分析结果可将12份材料分为五大类,且来源于同一地区的玉米材料趋向于聚为一类。【结论】Glb1基因具有较高的保守性,所选材料间Glb1基因序列存在一定的遗传多样性,利用Glb1基因序列可作为鉴定不同玉米地方品种的一种理想分子标记。  相似文献   

鸡 PD 1胞外区基因的克隆表达及其蛋白生物学活性鉴定     

李博文  孙国鹏  王爱国  岳锋  张艳芳  朱艳平  银梅  杨媛  郭东光  王选年 《河南农业科学》2014,(10)

为研究鸡程序性细胞死亡因子1（PD 1）胞外区的生物学活性,根据 GenBank 中的鸡 PD 1基因序列,经生物信息学比对分析,设计鸡 PD 1胞外区特异性克隆引物,扩增目的基因,连接原核表达载体 pET 28a（＋）,构建重组表达载体 pET 28a PD 1,转化至 Rosetta（DE3）大肠杆菌,并对IPTG 浓度、诱导温度、诱导时间进行优化,对表达产物进行 SDS PAGE 和 Western blot 分析,应用流式细胞术对所获得重组蛋白的生物学活性进行鉴定。结果显示,成功克隆了鸡 PD 1胞外区基因;SDS PAGE 和 Western blot 分析结果表明,37℃、0．1 mmol/L IPTG 诱导4 h 时,PD 1胞外区融合蛋白表达量最高,以包涵体形式存在;流式细胞术分析结果表明,PD 1胞外区重组蛋白具有一定的生物学活性。  相似文献   

多花水仙HMGS基因的克隆     

李科  何炎森  陈晓静 《福建农业大学学报(自然科学版)》2014,(3):289-294

以多花水仙花瓣抑制消减杂交文库获得的羟甲基戊二酰辅酶A合酶（HMGS）基因片段为基础,采用cDNA末端快速扩增（RACE）技术从黄花水仙2号和金盏银台中各克隆一条HMGS基因,两基因均含有一个1413bp的开放阅读框,编码470个氨基酸,但存在2个氨基酸差异．氨基酸序列分析表明：黄花水仙2号与葡萄、大豆、蓖麻和玉米HMGS基因的氨基酸相似系数分别为83％、82％、82％和81％．以水仙Actin基因为内参基因,采用荧光定量PCR方法分析HMGS基因在两品种的表达水平,结果表明：两品种HMGS基因的表达水平差异并不明显．  相似文献