Immunohistochemical evaluation of p53 expression with different antibodies in malignant canine tumours with or without p53 gene mutation     

A. Zacchetti  E. van Garderen  G. R. Rutteman 《Veterinary and comparative oncology》2007,5(2):108-118

Six monoclonal antibodies and a polyclonal antibody (CM1) were used to investigate the overexpression of p53 protein by immunohistochemistry (IHC) in six sarcomas and 21 mammary carcinomas from 27 dogs. IHC was compared with p53 gene mutation analysis performed on the same samples. Only the monoclonal PAb240, PAb421 and the CM1 antibodies were able to detect expression of canine p53 protein. CM1 was found to give the highest concordance (8/11) between positive expression of the p53 protein by IHC and the presence of a gene mutation. In the samples that were negative for p53 expression by IHC, but contained a p53 gene mutation according to DNA analysis, the mutation often affected the epitopes that could have been recognized by these antibodies. Only one out of 16 tumours without a p53 gene mutation had a weakly positive IHC result. These findings indicate that in these two types of canine tumours, IHC – particularly with CM1 – can detect many alterations in p53 expression owing to a gene mutation. False‐positive results were very infrequent.  相似文献   

Evaluation of serum iso-amylase in the normal dog using an improved electrophoretic technique     

J. W. Simpson  D. L. Doxey  G. Keay 《Veterinary research communications》1989,13(6):441-450

Six iso-amylase fractions are known to exist in human serum, three originating from the salivary glands and three from the pancreas. Although it is known that a different number and source of iso-amylase fractions occur in the dog, the routine detection of all the canine iso-amylase fractions has not been previously established. Earlier methods detected either two iso-amylase fractions or, in a proportion of cases, four fractions. A method is described which detects four iso-amylase fractions in 95% of normal canine serum samples. Trials have shown the method is reproducible and that freezing at -40°C has no effect on iso-amylase activity. The normal values for iso-amylase are recorded in 18 normal dogs.  相似文献   

MR EVALUATION OF INTRA AND EXTRACRANIAL EXTENSION OF NASAL ADENOCARCINOMA IN A DOG AND CAT     

Andra K.  Voges  DVM  Norman  Ackerman  DVM 《Veterinary radiology & ultrasound》1995,36(3):196-200

Nasal adenocarcinomas have been reported to extend into the cranial vault and cause neurologic disease. This report discusses a dog and cat with nasal adenocarcinoma which extended into the retrobulbar space, base of the cranial vault and adjacent soft tissues. The invasive characteristics of the neoplasms were documented by magnetic resonance imaging (MRI). Documentation of the extent of tumor is important if radiation therapy or surgery is planned. MRI was valuable in identifying extracranial and intracranial tumor invasion in these patients.  相似文献   

USE OF COMPUTED TOMOGRAPHY AND COMPUTED TOMOGRAPHIC MYELOGRAPHY FOR ASSESSMENT OF SPINAL TUMORAL CALCINOSIS IN A DOG     

Luc M. Van  Ham  DVM  Henri J. van  Bree  DVM  PhD  Mulenda  Tshamala  DVM  Hendrik  Thoonen  DVM 《Veterinary radiology & ultrasound》1995,36(2):115-118

Spinal tumoral calcinosis is reported in a Berner sennenhund puppy. The condition was manifested clinically as a non-ambulatory tetraparesis associated with neck pain. On survey radiographs there was a focal calcified mass at the atlantoaxial articulation. Computed tomography and computed tomographic myelography gave additional information on the extent of the mass and on the degree of spinal cord compression. The mass was removed surgically and the dog made a complete recovery.  相似文献   

USE OF TRANSCOLONIC ¹²³I-IODOAMPHETAMINE TO DIAGNOSE SPONTANEOUS PORTOSYSTEMIC SHUNTS IN 18 DOGS     

Philip D.  Koblik  DVM  MA  Chi-K  Yen  MD  William J.  Hornof  DVM  MS  Pamela  Whiting  DVM  Paul  Fisher  BS 《Veterinary radiology & ultrasound》1989,30(2):67-73

Transcolonic ¹²³I-Iodoamphetamine is rapidly absorbed across the colonic mucosa and binds to amine receptors in the liver and lungs. During the first ten minutes following colonic administration, a simple ratio of lung counts to lung counts plus liver counts provides an accurate estimate of the fraction of portal blood that bypasses hepatic sinusoids in dogs with portosystemic shunts. Studies were performed on 24 dogs with suspect portosystemic shunt. Shunt fraction values for 18 dogs with surgically confirmed portosystemic shunt were obviously higher than published values for normal dogs, and also significantly higher than values for the other six dogs, later confirmed to lack shunts. Postoperative studies were repeated on ten dogs with single shunt vessels 1–2 days after shunt closure. Total shunt ligation resulted in normal postoperative shunt fraction, whereas partial shunt ligation resulted in persistant elevation of shunt fraction. Transcolonic iodoamphetamine scintigraphy is noninvasive, easy to perform, and provides an accurate method to diagnose dogs with portosystemic shunt.  相似文献   

A SACRAL LESION RESEMBLING OSTEOCHONDROSIS IN THE GERMAN SHEPHERD DOG     

Johann  Lang  Dr. Med. Vet.  Hansjürg  Häni  Dr. Med. Vet Peter  Schawalder  Dr. Med. Vet 《Veterinary radiology & ultrasound》1992,33(2):69-76

More than 30% (21 of 65) of German Shepherd dogs with clinical signs of cauda equina compression had radiographic and pathologic abnormalities compatible with osteochondrosis of the sacral endplate. Most of these dogs had a defect in the dorsal part of the sacral endplate and a detached bone fragment in the vertebral canal. Similar lesions were also found in growing and young adult dogs without clinical signs. The dogs with clinical signs of cauda equina compression also had severe degenerative disc disease with protrusion of the lumbosacral disc and compression of the cauda equina, suggesting that the signs of cauda equina compression more likely were related to the secondary degenerative changes (disc protrusions) rather than the primary disease. Clinically normal German Shepherds with sacral osteochondrosis usually were younger than 18 months, the dogs with cauda equina compression and sacral osteochondrosis older than 18 months (mean age 4.8 years). On the average, these dogs were two years younger as compared to dogs with cauda equina compression without sacral osteochondrosis. Male dogs are more often affected than females (5:1). There is a breed predisposition: in dogs other than German Shepherds, osteochondrosis of the sacral endplate seems to be extremely rare.  相似文献   

ERG findings in three hypothyroid adult dogs with and without levothyroxine treatment     

Philippe Durieux  F. Rigaudière  J.-F. LeGargasson  S. G. Rosolen 《Veterinary ophthalmology》2008,11(6):406-411

Purpose To evaluate the effects of levothyroxine (LTh) on the electroretinogram (ERG) of adult dogs. Material and methods Binocular, full field photopic and scotopic ERGs were recorded from an anesthetized Maltese Bichon cross (MB), a Yorkshire Terrier (YT) and a Shetland Sheepdog (SS) affected with hypothyroidism and treated with a daily dose of LTh at 20 µg/kg. The photopic ERGs were evoked to 12 different intensities ranging from 0.81 to –2.19 log cd.s/m² and presented under photopic conditions in order to assess (from the derived luminance-response curves) Vmax and b : a amplitude ratio parameters. Photopic flicker ERGs were obtained at 30 Hz. The scotopic ERGs (intensity: –3.09 log cd.s/m²) were recorded while the retina was dark-adapting and after 32 min of dark adaptation. This procedure was performed on two separate sessions: following a 3-day interruption of LTh treatment (S1) and following 30 days without interruption of LTh treatment (S2). Results The mean photopic a-wave peak times were 9.8 ms at S1 and 5.0 ms at S2, respectively. The mean photopic b-wave peak times were 23.3 ms at S1 and 11.5 ms at S2, respectively, and the mean scotopic b-wave peak times (after 32 min of dark adaptation) were 45.2 ms at S1 and 26.0 ms at S2, respectively. No other significant ERG changes were observed. Conclusion Our results indicate that a dose of 20 µg/kg of LTh given to adult dogs was accompanied by a marked peak time shortening of both photopic and scotopic ERGs, without affecting other ERG parameters.  相似文献   

MENINGEAL ENHANCEMENT ON MAGNETIC RESONANCE IMAGING IN 15 DOGS AND 3 CATS     

Linda M.  Mellema  DVM  Valerie F.  Samii  DVM  Karen M.  Vernau  DVM  Richard A.  Lecouteur  BVSC  PhD 《Veterinary radiology & ultrasound》2002,43(1):10-15

A retrospective study of 15 dogs and three cats was done to characterize the appearance of meningeal enhancement on magnetic resonance (MR) images of the brain, and to correlate this appearance with its underlying cause. Two patterns of meningeal enhancement (pial and dural) were identified. Enhancement of the pia mater was evident in four dogs and one cat, while enhancement of the dura mater was seen in 11 dogs and 2 cats. A variety of causes of meningeal enhancement were identified, including bacterial and cryptococcal meningitis, plasmacytic meningitis with associated subdural fluid accumulation, granulomatous meningoencephalomyelitis, inflammation secondary to otitis interna, feline infectious peritonitis, and neoplasia. The present study confirms that pial or dural meningeal enhancement may be present on MR images of the brain of dogs or cats in association with a variety of central nervous system diseases. A larger prospective study is required to further establish the incidence of specific patterns of meningeal enhancement seen in association with specific diseases.  相似文献   

犬空肠替代尿道术的研究     

崔雯  姜先明  朱珊珊  张中文 《动物医学进展》2007,28(Z1):11-14

采用自体器官移植的思路,探索用自体部分带蒂空肠代替尿道的手术方法,以解决实际中动物因尿道结石、尿道损伤等所致的自身尿道无法正常排泄尿液的情况.经过术后观察,犬的各项生理指标均趋于正常.结果表明,此段带蒂空肠可存活,并可代替尿道,保证机体正常的尿液排泄,为犬的尿道再造术提供了一种新的方法.  相似文献   

犬细小病毒病的诊治报告     

夏绿蓉  唐晓玲  邱伟海 《经济动物学报》2007,11(4):246-248

2007年3月一养殖户养殖的圣伯纳犬发生疫病,根据发病情况、临床特征、剖检变化、实验室诊断等,确诊为犬细小病毒病,立即对该病采取了相应的治疗措施,取得了较好的效果。  相似文献