猪增生性肠炎的研究进展   总被引：1，自引：1，他引：0  

余彦国  张瑾 《畜牧兽医杂志》2009,28(3):33-36

猪增生性肠炎（porcine proliferative enteritis,PPE）,又称猪增生性肠病（porcine roliferative nteropathy,PPE）,是以回肠和结肠隐窝内未成熟的肠细胞发生腺瘤样增生为特征的猪的一种肠道综合症。本文主要概述其痛原学、流行病学、发病机理、临床症状、病理变化、诊断以及防治等方面的研究现状。  相似文献   

表达绿色荧光蛋白的鸭肠炎病毒gE基因转移载体构建     

乌伊罕  赵妍  刘晓玫  张智慧  马波  王君伟 《中国动物检疫》2009,26(6):27-29

本实验利用PCR方法扩增DEVC-KCE株gE基因片段(包含gI基因及其侧翼序列),扩增产物克隆入pGEM-T载体测序后亚克隆至pUC19 EcoRI、SalI位点间,获得pUC-gE。将增强型绿色荧光蛋白表达盒插入pUC-gE BamHI位点,构建转移载体pUC-gE-EGFP。该转移载体有7个单一酶切位点可供外源基因插入,上下游侧翼分别为1.57Kb和1Kb。将转移载体pUC-gE-EGFP转染鸭胚成纤维细胞(DEF),观察到绿色荧光,说明EGFP基因获得有效表达,为开发以鸭肠炎病毒为载体的多价、多联基因工程疫苗提供了物质基础。  相似文献   

鸭肠炎病毒gD基因胞外区的原核表达及在感染鸭胚成纤维细胞中的定位     

刘琰  徐凝  付培芬  母晓宇  董井泉  马波  王君伟 《畜牧兽医学报》2012,43(6):922-927

以本实验室获得的鸭肠炎病毒(DEV)C-KCE株US区基因序列(GenBank登录号EU714267)为基础PCR扩增出gD基因胞外区(gDw),将其定向克隆至原核表达载体pET-30a(+)并转化至大肠杆菌RosettaTM(DE3)pLys。经IPTG诱导表达,SDS-PAGE分析表明gD基因胞外区在大肠杆菌中获得与预期大小相符的表达蛋白。重组蛋白纯化后免疫家兔制备多克隆抗体,血清琼扩效价达1∶16,噬斑减数中和试验测定血清中和抗体效价达1∶64。通过免疫荧光技术首次对gD进行亚细胞定位检测,结果表明DEV感染鸭胚成纤维细胞(DEF)后4h近核区域细胞质内首先开始出现荧光,12h以后显著增加,明显的点状绿色荧光广泛存在于胞质中。本研究为进一步开展DEV gD的功能研究提供了重要数据和材料。  相似文献   

北京鸭源鹅出血性多瘤病毒的分子检测     

姜甜甜  张大丙 《中国兽医杂志》2012,48(6):3-6

鹅出血性多瘤病毒(Goose hemorrhagic polyomavirus,GHPyV)是鹅出血性肾炎肠炎的致病病原,迄今为止,国际上已从鹅、番鸭和半番鸭中检测到GHPyV。为了解北京鸭是否存在GHPyV的感染,本研究从北京、山东、河北、河南等地的北京鸭种鸭场采集肝脾组织样品68份,并用GHPyV特异性PCR进行了检测。结果显示,从5份肝脾组织样品中检出GHPyV的VP1基因。选一株北京鸭源GHPyV(106株)测定了基因组序列。测序结果显示,北京鸭源GHPyV基因组长度为5 254bp,相对于德国鹅源GHPyV毒株Germany 2001,106株基因组在其编码区共有10个碱基位发生突变,但只有2个碱基位的突变引起氨基酸的置换;此外,106株基因组的非编码区存在2个核苷酸的缺失。对不同水禽来源的GHPyV基因组序列进行比较的结果表明,GHPyV的基因组序列具有高度保守性。由于GHPyV可感染鹅、番鸭、半番鸭和北京鸭,表明该病毒具有较广泛的宿主范围。  相似文献   

Intestinal mucosal immune response against virulent duck enteritis virus infection in ducklings     

Xuefeng Qi  Xiaoyan Yang  Anchun Cheng  Mingshu Wang  Dekang Zhu  Renyong Jia  Qihui Luo  Xiaoyue Chen 《Research in veterinary science》2009,87(2):218-225

Duck virus enteritis (DVE) is an acute and contagious herpes virus infection of duck, geese and swans with high morbidity and mortality. The development of specific mucosal immune system against duck enteritis virus (DEV) infection for ducks has been hindered by a lack of knowledge concerning the purification of immunoglobulin A (IgA) of duck. In the present work, the method for purification of duck immunoglobulin A was developed, and the induction of intestinal mucosal immune responses against DEV was studied by orally infected ducklings with virulent DEV. The results showed that a continuous increased DEV DNA levels were observed in blood and various organs examined of orally infected ducklings throughout the infection, which was accompanied by the development of infection in ducklings from mild progressed to severe pathological lesions. Furthermore, a marked increased level of DEV-specific IgA and IgG antibodies in bile, serum and the intestinal tract, as well as the density of IgA⁺ cells in intestine were detected between 1 and 12 days p.i., followed by a drastic reduction of the antibody levels and the density of IgA⁺ cells at 15 days p.i. The results indicate that the DVE infection can stimulate both IgA-dominated antibody immune responses in the intestinal tract, and IgG-dominated antibody systemic immunity in the serum of ducklings orally inoculated with virulent DEV. The severe lesions of the villus epithelial cells and the lymphoid organs can suppress the intestinal mucosal immune responses.  相似文献   

A scoring index for disease activity in canine inflammatory bowel disease     

Jergens AE  Schreiner CA  Frank DE  Niyo Y  Ahrens FE  Eckersall PD  Benson TJ  Evans R 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(3):291-297

The clinical course of inflammatory bowel disease (IBD) in dogs is characterized by spontaneous exacerbations and remissions, which makes assessment of disease burden difficult. The objectives of this study were to develop a scoring system for evaluation of canine IBD activity and to validate this scoring method by correlating it to objective laboratory and histologic indices of intestinal inflammation. Fifty-eight dogs with IBD were evaluated prospectively and compared to 9 disease-free control dogs. Clinical disease activity was quantified by a simple scoring system, the canine IBD activity index (CIBDAI), and compared to serum concentrations of C-reactive protein (CRP), haptoglobin (HAP), alpha-acid glycoprotein (AGP), and serum amyloid A (SAA), as well as histology scores derived from endoscopic biopsy specimens. Forty-six dogs were available for a reevaluation of the CIBDAI, CRP HAP, and AGP, and 34 dogs had repeat analysis of SAA performed after medical therapy. Serum concentrations of CRP were significantly (P < .02) increased in dogs with CIBDAI scores > or = 5 (mild disease activity or greater) compared to controls. Among IBD dogs, the CIBDAI showed good correlation (r = 0.82, P < .0001) to both histology and HAP scores, but CRP also was a strong co-correlate of disease activity. The IBD dogs showed significantly (P < .0001) decreased CIBDAI and CRP values but significantly (P < .0001) increased HAP concentrations after medical therapy compared to pretreatment values. We conclude that the CIBDAI is a reliable measure of inflammatory activity in canine IBD and that CRP is suitable for laboratory evaluation of the effect of therapy in these patients.  相似文献   

人工感染鸭病毒性肠炎急性病例细胞凋亡的电镜研究     

袁桂萍  程安春  汪铭书  周毅  刘菲  郭宇飞  韩晓英  廖永洪  徐超  周伟光  文明  贾仁勇  陈孝跃 《畜牧兽医学报》2005,36(11):1174-1177

应用透射电镜和超薄切片技术观察鸭病毒性肠炎病毒（Duck Enteritis Virus，DEV）CH强毒株人工感染成年鸭各组织器官的形态学特征。结果表明，脾脏、胸腺、法氏囊、十二指肠固有层中淋巴细胞除了坏死变化外还有很明显的凋亡变化，两者往往同时存在。疾病过程中，淋巴细胞凋亡数量明显增多。凋亡细胞的形态学改变是细胞体积缩小，细胞器结构正常，染色质初期浓集成团，聚集于核膜的周边，随后出现染色质凝聚，核碎裂以及凋亡小体形成等现象。淋巴细胞的坏死和凋亡共同造成了淋巴细胞的大量损耗，淋巴细胞的这种变化可能在鸭病毒性肠炎的发病机制中起着重要的作用。研究结果表明DEV急性感染可诱导成年鸭体内淋巴细胞的凋亡。  相似文献   

鸭病毒性肠炎病毒CH强毒株感染鸭胚成纤维细胞的超微结构观察   总被引：4，自引：0，他引：4  

郭宇飞  程安春  汪铭书  周伟光  文明  贾仁勇  袁桂萍  徐超  韩晓英  廖永洪 《中国兽医学报》2005,25(6):632-635

通过超薄切片和透射电子显微镜技术对鸭病毒性肠炎病毒（DEV）CH强毒株在鸭胚成纤维细胞（DEF）中的形态结构进行了研究。结果发现，DEVCH强毒株病毒核酸呈圆形颗粒状，直径35～45nm，在胞核内常集中分布；病毒核衣壳呈圆形．直径90～100nm．在胞核和胞浆内都有分布；DEV核衣壳可根据所含核酸形态的差异分为空心核衣壳、内壁附有颗粒型核衣壳、同心圆形核衣壳和实心核衣壳；成熟的病毒粒子具有囊膜和皮层结构．呈圆形．直径150～300nm，存在于胞浆空泡内；DEV可在DEF中分别形成胞浆内和胞核内包涵体结构；伴随子代病毒在细胞内的出现，胞浆内迁出现豆英状、马蹄形、半圆形、圆形、同心圆形等与病毒发生有关的电子致密结构。  相似文献   

Necrotic enteritis-producing strains of Clostridium perfringens displace non-necrotic enteritis strains from the gut of chicks   总被引：1，自引：0，他引：1  

Barbara AJ  Trinh HT  Glock RD  Glenn Songer J 《Veterinary microbiology》2008,126(4):377-382

We inoculated broiler chicks with mixtures of Clostridium perfringens strains to investigate the single strain dominance observed in natural cases of necrotic enteritis (NE) [Nauerby, B., Pedersen, K., Madsen, M., 2003. Analysis by pulsed-field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens. Vet. Microbiol. 94, 257-266]. Pre-inoculation bacteriologic culture of chick intestines yielded up to six pulsed-field gel electrophoresis (PFGE) types of C. perfringens. Birds developed typical NE lesions in response to administration (2x per day for 4 days) of a combined inoculum comprising one NE strain (JGS4143, PFGE pattern 8) and four non-NE strains (from piglet necrotizing enteritis, chicken normal flora, human gas gangrene, and bovine neonatal enteritis). After inoculation commenced, only the NE strain was recovered through the first post-inoculation day, in spite of intense efforts to recover pre-challenge flora strains and the other challenge strains. Thereafter, pre-inoculation and previously undetected PFGE types were found, and JGS4143 became undetectable. Birds inoculated simultaneously with five NE strains (from disease in chickens or turkeys, and including JGS4143) also developed lesions, but again only JGS4143 was recovered through the 1st day post-challenge. At that time, birds began to be repopulated with pre-challenge PFGE types. Two NE strains (JGS4143 and JGS4064) produced bacteriocins, which inhibited each other and normal flora strains (n=17), while normal flora strains inhibited neither NE strains nor each other. Thus, it appears that naturally occurring dominance of the gut by NE strains can be reproduced experimentally. Bacteriocins directed against normal flora could possibly provide the necessary advantage, although inhibition of one NE strain by another suggests that other factors may be partially or completely responsible for the dominance.  相似文献   

Complications and survival associated with surgical compared with medical management of horses with duodenitis-proximal jejunitis     

Underwood C  Southwood LL  McKeown LP  Knight D 《Equine veterinary journal》2008,40(4):373-378

REASONS FOR PERFORMING STUDY: Based on clinical observation, it is hypothesised that horses with duodenitis-proximal jejunitis (DPJ) that are treated surgically have a shorter duration, smaller volume, and slower rate of nasogastric reflux (NGR) compared to horses treated medically, are more likely to develop diarrhoea than medically managed cases, and have a higher incisional infection rate than a sample population of horses undergoing abdominal exploration for gastrointestinal disease other than DPJ. OBJECTIVES: To compare: 1) duration, volume and rate of NGR and the percentage of horses with diarrhoea between medically and surgically treated DPJ cases; and 2) incisional infection rate in horses with DPJ undergoing abdominal exploration to a sample population of horses undergoing abdominal exploration for gastrointestinal disease other than DPJ. METHODS: Medical records of cases with DPJ diagnosed 1995-2006 were reviewed. Information obtained included subject details, presenting clinical findings, treatment category (medical/surgical), complications (diarrhoea, incisional infection), and outcome (survival/nonsurvival). Data were analysed using a Chi-squared test and a mixed model analysis of variance. Level of significance was P<0.05. RESULTS: Compared to medical cases, surgical cases had significantly decreased survival, a longer duration and larger total volume of NGR, and were more likely to develop diarrhoea. The incisional infection rate for horses with DPJ undergoing abdominal exploration was 16% compared to 7% for the sample population of horses. CONCLUSIONS: Surgical treatment of horses with DPJ did not lead to resolution of NGR faster than medical treatment. Surgical cases were more likely to develop diarrhoea and did not have a significantly higher incisional infection rate than the sample population.  相似文献