气-质联用(GC/MS)法测定烟草生物碱的方法优化   总被引：1，自引：0，他引：1  

肖遂  周冀衡 《湖南农业大学学报(自然科学版)》2010,36(1):22-26

采用气相色谱-质谱法(GC/MS),通过优化相关技术参数建立了能够快速准确测定烟草中烟碱和微量生物碱(降烟碱、麦斯明、假木贼碱和新烟草碱)含量的方法.经正交试验确定了超声提取和有机溶剂萃取前处理方法的最佳条件,结果表明:烟碱、降烟碱、麦斯明和假木贼碱的线性范围分别为20～600、30～300、3.2～12、16～60mg/L,检测限分别为0.11、0.09、0.02和0.04mg/kg,回收率介于88.5%～105.4%,相对标准偏差(n=5)介于2.4%～9.2%.该方法具有检测烟草生物碱灵敏度高和重现性好的特点,且操作简便,解决了已有方法存在的生物碱各个组分提取不完全和含量极低的麦斯明检测效果不理想等问题.  相似文献   

Psammaceratin A: A Cytotoxic Psammaplysin Dimer Featuring an Unprecedented (2Z,3Z)-2,3-Bis(aminomethylene)succinamide Backbone from the Red Sea Sponge Pseudoceratina arabica     

Diaa T. A. Youssef  Hani Z. Asfour  Lamiaa A. Shaala 《Marine drugs》2021,19(8)

Bioassay-guided partition of the extract of the Red Sea sponge Pseudoceratina arabica and HPLC purification of the active fraction gave a psammaplysin dimer, psammaceratin A (1), along with psammaplysin A (2). The dimer comprises two units of psammaplysin A (2) connected via the terminal amines with an unprecedented (2Z,3Z)-2,3-bis(aminomethylene)succinamide moiety, and it represents the first dimer to be identified among the psammaplysin family. Data from 1D- and 2D-NMR and HRMS supported the chemical structures of the compounds. Psammaceratin A (1) and psammaplysin A (2) exhibited significant growth inhibition of HCT 116, HeLa, and MBA-MB-231 cells down to 3.1 μM.  相似文献   

Inhibitory activities of venom alkaloids of Red Imported Fire Ant against Clavibacter michiganensis subsp. michiganensis in vitro and the application of piperidine alkaloids to manage symptom development of bacterial canker on tomato in the greenhouse     

Shezeng Li  Xixuan Jin  Jian Chen  Shien Lu 《国际虫害防治杂志》2013,59(2):150-156

Tomato bacterial canker caused by Clavibacter michiganensis subsp. michiganensis (CMM) is a highly destructive disease that has caused major economic losses in tomato production worldwide. In seeking disease management alternatives, the inhibitory activity of alkaloids extracted from the Red Imported Fire Ant was studied in the laboratory and the greenhouse. Piperidine and piperideine alkaloids each significantly inhibited CMM growth on nutrient agar plates. The inhibitory activity of piperidine alkaloids was stable at 4 ^° C and 22 ^° C for 12 weeks and at 54 ^° C for 4 weeks. The growth of CMM was negatively correlated with the concentration of piperidine alkaloids in nutrient broth. In the greenhouse, piperidine alkaloids also significantly reduced the symptom development on two tomato cultivars, Better Boy and DRK7018F1. This is the first demonstration that piperidine and piperideine alkaloids from the Red Imported Fire Ant are highly inhibitory against a plant-pathogenic bacterium, viz. CMM. Piperidine alkaloids could provide satisfactory management of CMM bacterial canker on tomato seedlings in the greenhouse. Our findings may lead to the development of a new group of bactericides.  相似文献   

博落回中季铵类苯并啡啶类生物碱的提取及其硫酸氢盐的抗菌活性和急性毒性     

王斌  程辟  陈巨莲  曾建国 《农药学学报》2013,15(3):299-304

采用酸提碱沉的方法,从博落回中提取并制备了苯并啡啶类生物碱(QBAs)——血根碱(sanguinarine)和白屈菜红碱(chelerythrine)的硫酸氢盐;采用高效液相色谱法测定了2种生物碱的含量;初步测定了其硫酸氢盐对几种常见病原菌的抗菌活性,并对其急性毒性进行了研究。结果表明:所制备的QBAs硫酸氢盐中血根碱和白屈菜红碱的质量分数均在60%以上。生物测定结果表明,该生物碱的硫酸氢盐对葡萄叶斑病菌Cercospora viticola、葡萄黑豆病菌Elsinoe ampelina、葡萄炭疽病菌Colletotrichum gloeosporioides、葡萄褐斑病菌Cercospora viticola、稻瘟病菌Pvricularia oryzae、小麦赤霉病菌Gibberella zeae和辣椒疫霉Phytophythora capsici均具有较强的抑制作用,EC50值在3.35～10.08 μg/mL之间。其急性毒性属低毒,对皮肤有弱致敏性,但对眼睛有重度刺激性。博落回中QBAs硫酸氢盐制备方法稳定,具有较强的抗菌活性,可考虑开发成低毒、环境友好型植物源杀菌剂。  相似文献   

PEG6000对长春花幼苗期的胁迫效应和生物碱含量的影响     

刘英  张衷华  李德文  赵冬梅 《华中农业大学学报》2013,32(5):40-44

在温室培养条件下,研究不同体积分数聚乙二醇(polyethylene glycol,PEG)6000处理下长春花幼苗期的胁迫效应以及对文多灵、长春质碱和长春碱等生物碱含量的影响。结果表明:相同体积分数PEG6000处理下,随着胁迫时间的延长,叶片相对含水量呈先降低后上升的趋势,脯氨酸含量呈缓升到急升的趋势。综合二因素的差异显著分析,选择35%(V/V)PEG6000模拟干旱胁迫处理长春花,与对照相比,干旱胁迫的叶片中的过氧化物酶活性先增强后降低;文多灵(vindoline,VIN)含量先升高后下降,长春质碱(catharanthine,CAT)含量先下降后升高,而长春碱(vinblastine,VBL)含量逐渐升高,24 h达到峰值为0.168 0±0.003 6 mg/g（鲜质量）。  相似文献   

杭州地区有机毛峰品质级别与化学成分相关性研究     

邓威威  马林龙  顾辰辰  金阳 《安徽农业大学学报》2013,40(4):559

采用高效液相色谱法对杭州地区有机毛峰茶叶4个等级（特级、一级、二级、三级）的氨基酸、嘌呤碱、儿茶素进行检测,并结合其感官审评分析,对杭州地区有机毛峰茶叶品质与其化学成分的关联性进行研究。结果表明,该毛峰茶叶中氨基酸、嘌呤碱总量随着品质级别的降低而减少;儿茶素指数除了特级比一级略低外,有随茶叶品质级别的降低而降低,儿茶素与氨基酸比值有随茶叶品质级别的降低而增加的规律;基于外形、汤色、香气、滋味、叶底感官审评因子来看,该批次的毛峰茶叶有随着等级呈现出与品质的正相关性。  相似文献   

Polycitorols A and B,New Tricyclic Alkaloids from an Ascidian     

Hamad H. Issa  Junichi Tanaka  Rachmaniar Rachmat  Andi Setiawan  Agus Trianto  Tatsuo Higa 《Marine drugs》2005,3(3):78-83

Two new tricyclic alkaloids, polycitorols A (1) and B (2) have been isolated along with the known lepadiformine (3) from a marine ascidian of the family Polycitoridae. The structures of the new compounds were elucidated by analysis of NMR data and comparison with those of 3 and other related compounds [1–5]. Compounds 1 and 2 are closely related to cylindricines A and B, lacking C-4 oxygenation found in cylindricines and having a butyl instead of a hexyl appendage. NOE experiments on compounds 1 and 2 suggested the A/B ring fusion to be cis.  相似文献   

Antimicrobial and Antimycobacterial Activity of Cyclostellettamine Alkaloids from Sponge Pachychalina sp.     

Jaine H. H. L. de Oliveira  Mirna H. R. Seleghim  Christoph Timm  Achim Grube  Matthias K?ck  Gislene G.F. Nascimento  Ana Claudia T. Martins  Elissa G. O. Silva  Ana Olívia de Souza  Paulo R. R. Minarini  Fabio C. S. Galetti  Célio L. Silva  Eduardo Hajdu  Roberto G. S. Berlinck 《Marine drugs》2006,4(1):1-8

Cyclostellettamines A – F (1 – 6) isolated from the sponge Pachychalina sp. and cyclostellettamines G - I, K and L (7 – 11) obtained by synthesis were evaluated in bioassays of antimicrobial activity against susceptible and antibiotic-resistant Staphylococcus aureus, Pseudomonas aeruginosa and antibiotic-susceptible Escherichia coli and Candida albicans, as well as in antimycobacterial activity against Mycobacterium tuberculosis H37Rv bioassays. The results obtained indicated that cyclostellettamines display different antimicrobial activity depending on the alkyl-chain size, suggesting that, if a mechanism-of action is implied, it is dependent on the distance between the two pyridinium moieties of cyclostellettamines.  相似文献   

狗舌草生物碱成分分析   总被引：8，自引：0，他引：8  

王建华  王跃虎  司红丽 《西北农林科技大学学报(自然科学版)》2004,32(1):93-95

为了更好地评价狗舌草的药理活性和毒性,利用溶剂萃取法从狗舌草中得到总生物碱,通过色谱和波谱技术对总生物碱进行了分离和鉴定。从TLC及GC-MS判断,狗舌草中至少含有4个双稠吡咯啶生物碱(PAs),GC-MS确定化合物 ～ 分别为千里光宁、千里光非灵和全缘千里光碱。经柱层析和制备性TLC得化合物 ,MS,IR及13CNMR鉴定其为当归酰天芥菜定;化合物 水解产物之一经制备性TLC分离得化合物 ,MS鉴定为天芥菜定。这些化合物的存在说明狗舌草可能具有抗肿瘤活性,并提示其对肝脏的毒性不可忽视。  相似文献   

雷公藤总生物碱分离及杀虫活性研究   总被引：13，自引：1，他引：13  

罗都强  冯俊涛  胡瓒 《西北农林科技大学学报(自然科学版)》2001,29(2):61-64

提取、分离了雷公藤 (Tripterygium wilfordii Hook)根皮中总生物碱 ,并对其杀虫活性和作用方式进行了系统的生物测定。结果表明 ,雷公藤总生物碱对 5龄菜青虫有很强的麻醉、拒食和毒杀作用 ;拒食中浓度AFC5 0 为 2 75 .5 3m g/L,毒杀中浓度 L C5 0 为 77.86 m g/L,麻醉中量 ND5 0 为 2 .2 9μg/g;雷公藤总生物碱无触杀和熏蒸作用。试虫中毒症状主要为幼虫表皮出现黑斑 ,形成黑斑部分不能脱去旧表皮 ,最终死亡  相似文献