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采用常温状态和冷诱导状态两类地方民猪样本获得用于筛选民猪抗寒性状相关基因基因芯片数据,针对两类样本芯片抗寒性状相关基因提取问题,提出错分风险最小化特征搜索模型,通过设定算法参数筛选出抗寒性状相关基因集合,这些抗寒性状相关基因对于两类样本分类特征好,比较三种经典分类方法都获得稳定高效分类准确率,特征筛选过程获得抗寒相关基因具有较强分类效能,对基因生物学功能进行初步研究,这些基因参与功能过程是下一步要研究目标. 相似文献
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基因芯片分析水稻灌浆期籽粒淀粉合成相关基因的表达 总被引：2，自引：0，他引：2
水稻Oryza sativa灌浆过程是籽粒胚乳细胞蔗糖转变成淀粉的过程.为了解这期间的基因表达变化,研究利用Affymetrix水稻基因芯片分析了水稻籽粒灌浆3和9 d的基因表达差异,并着重分析了淀粉合成代谢相关基因的表达模式.结果表明,在检测到信号的59个淀粉合成相关基因中,灌浆后9 d相比3 d表达丰度上调2倍的基因有20个,包括SUS2、AGPS2、AGPL2、GBSSⅠ、SSⅡa、BEⅠ等,这类基因对水稻籽粒淀粉合成和灌浆起了至关重要的作用.灌浆后9 d相比3 d表达丰度下调2倍的基因有12个,包括CIN2、SPS3、AGPL1、GBSSⅡ、SSⅢb等,这类基因可能参与胚乳细胞基本结构的形成以及初始淀粉粒的产生. 相似文献
95.
湖羊不同花纹皮肤组织差异表达基因的筛选 总被引：3，自引：3，他引：0
【目的】旨在了解中国特有的白色羔皮羊品种—湖羊不同花纹及其毛囊形成的分子遗传学机理。【方法】利用安捷伦基因芯片技术筛选初生湖羊大花和小花皮肤组织差异表达基因。【结果】差异表达分析显示,3对大花和小花组分别筛选出1 069、2 071和3 879个差异表达基因,共有137个共同基因;差异基因经GO分类显示,主要参与细胞分化、增殖、凋亡、发育、免疫应答、离子转运等生物学过程;另外,所验证的4个差异表达基因的荧光定量PCR结果与芯片结果基本一致。【结论】筛选出了BMP7、MMP2、SNAI1、SFXN1、CDKNIC、MT3、POU1F1等对湖羊大花、小花性状形成可能具有重要影响的候选基因。 相似文献
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Seventy‐two pigs at 34.4 kg body weight (BW) were allotted to two treatments with six replicates/treatment and six pigs/pen: the CON (negative control, no added selenium (Se)) and the OS (0.36 mg/kg added selenium from selenium‐enriched yeast). Pigs were fed until 130 kg BW. The CON diet contained 0.18 mg/kg indigenous Se whereas the OS diet contained 0.54 mg/kg Se. Blood samples were collected at 130 kg BW and further processed for microarray analysis, prepared with 885 genes related to immune function of pigs. Among those, 28 genes related to improved immune status and innate immunity were up‐regulated (P < 0.05) in leukocytes from Se‐fed pigs and those include major histocompatibility class I (> 1.66), arginase I (> 1.27), integrin beta‐1‐subunit (> 1.20), toll like receptor 2 (> 1.12) and double‐stranded RNA‐dependent protein kinase. However, 24 genes including tissue factor (< 4.70), serum amyloid A‐2 protein (< 3.11) and p27Kip1 (< 1.42) were down‐regulated (P < 0.05) in leukocytes from Se‐fed pigs. Expression of four selected genes was validated using quantitative PCR (qPCR) showing significant correlation between mircroarray analysis and qPCR analysis. This study indicates that a long‐ term dietary supplementation (0.3%) of organic Se improves the expression of genes that are related to enhanced immunity of pigs. 相似文献
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全基因组分析低氮胁迫下水稻剑叶光合相关基因表达变化 总被引：3，自引：1，他引：2
【目的】研究水稻剑叶叶绿素和光合特性及相关基因对低氮胁迫的响应,为提高水稻对氮肥的吸收和利用效率奠定分子基础。【方法】利用Agilent 4×44K芯片全基因组研究低氮胁迫处理下,2个不同叶绿素含量水稻齐穗期剑叶的光合相关基因表达的变化。【结果】SN19-6和丰锦剑叶的叶绿素含量和净光合速率在低氮胁迫下均有所下降,但SN19-6 2个指标下降的幅度明显较小。低氮胁迫处理与对照处理相比,超绿水稻SN19-6剑叶共有41个光合相关基因表达发生变化(14个在转录水平上下调表达,27个在转录水平上上调表达)。丰锦剑叶有29个光合相关基因表达发生变化(15个在转录水平上下调表达,14个在转录水平上上调表达)。低氮胁迫响应光合相关基因表现出品种特异性,超绿水稻SN19-6有29个为特异响应的,丰锦有17个特异响应的。2水稻品种(系)低氮胁迫响应的光合相关基因有12个重叠的,其中,5个在转录水平上上调表达,7个下调表达。【结论】在低氮胁迫下,水稻剑叶光合相关基因的表达发生变化,不同叶绿素含量水稻品种(系)中的表达既表现特异性,也存在部分重叠。 相似文献
99.
C. Camps C. Kappel P. Lecomte C. Léon P. Coutos‐Thévenot S. Delrot E. Gomès 《Plant pathology》2014,63(2):323-333
Eutypa lata is the causal agent of eutypa dieback, a highly damaging trunk disease affecting all grape‐growing areas, with currently neither an efficient curative treatment nor an early non‐destructive diagnostic method. The present work was carried out to discover grapevine genes expressed in response to the presence of E. lata that could be useful to develop an early (before visible foliar symptoms) and non‐destructive (using grapevine leaves) diagnostic tool. Microarray analyses were carried out from (i) infected plants showing characteristic E. lata foliar and vascular symptoms and positive pathogen recovery from vascular lesions (S+R+), (ii) infected plants showing no symptoms (S?R+), and (iii) symptomless plants with negative pathogen recovery (S?R?). Vineyard and greenhouse‐grown plants, naturally or artificially infected respectively, and uninoculated controls were characterized and leaf RNA was hybridized with 15k operon grapevine oligonucleotide microarrays. Among the grapevine genes differentially expressed between S?R+ and S?R? plants in greenhouse and vineyard conditions, 10 were highlighted as robust candidate genes for diagnosis: seven were specifically involved in response to infection and three were associated with symptom absence. Five were confirmed to be effective diagnostic marker genes usable in a qRT‐PCR‐based test performed on RNA extracted from grapevine leaves cultivated in either greenhouse or vineyard conditions. Furthermore, their expression profiles in response to infection with E. lata or other major grapevine fungi (Erysiphe necator, Plasmopara viticola, Botrytis cinerea) could be distinguished. The usefulness of these genes to develop an early and non‐destructive method for diagnosis of E. lata infection is discussed with regard to the advantages and drawbacks of previous E. lata diagnostic studies. 相似文献
100.
多样性芯片技术(DArT)是近年新发展起来的一种遗传标记技术, 具有高通量和低成本的显著特点, 克服了以往以跑电泳凝胶为主的标记技术产量低以及成本高、耗时长、自动化程度低以及依赖核酸序列信息等缺点, 是一种比较理想的遗传标记技术。对该技术的技术原理及主要操作、技术特点和应用做了较详细的分析。 相似文献