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81.
为了解贵州地方山羊肌肉生长抑制素基因(Myostatin)的表达部位及组织分布模式,比较各组织中Myostatin基因表达的品种间差异,以β-actin作为内参基因,应用Taq Man实时荧光定量方法检测了黔东南小香羊、贵州白山羊、贵州黑山羊、黔北麻羊等贵州地方山羊品种以及南江黄羊的肝、肾、心、肺、背最长肌、半膜肌、皮下脂肪组织中Myostatin基因mRNA的表达水平。结果显示:肝和皮下脂肪中Myostatin基因的表达水平较高,肾和肺次之,半膜肌、背最长肌和心等肌肉组织中的表达水平相对较低;而半膜肌和背最长肌中Myostatin基因的表达水平高于心肌。肝和皮下脂肪Myostatin基因的表达在各品种间差异不显著;心、肺、肾、背最长肌和半膜肌中Myostatin基因的表达,贵州白山羊显著高于黔东南小香羊和黔北麻羊;肺、背最长肌和半膜肌中,Myostatin基因的表达贵州白山羊显著高于贵州黑山羊和南江黄羊。可见,贵州地方山羊心、肝、肺、肾、背最长肌、半膜肌和皮下脂肪中均有Myostatin基因表达,其组织表达水平总体为肝和皮下脂肪肾和肺半膜肌、背最长肌和心(肌),且多数组织中Myostatin基因的表达存在品种差异。 相似文献
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猪肌生成抑制素基因myostatin(MSTN)的cDNA在去除信号肽后,对成熟蛋白编码序列PCR扩增出1.2kb片段,将该片段与pMD18-T载体连接,转化JM109受体菌细胞,筛选阳性克隆,并测序分析,结果表明其与设计序列完全一致。将该克隆载体的质粒DNA用带有BamH 和Sal 内切酶识别序列的另1对引物进行PCR扩增,将回收的1.2kbPCR目的片段定向克隆到pET28a(+)表达载体上,成功地构建了猪肌生成抑制素成熟蛋白编码的原核表达载体。对成功构建的表达载体阳性克隆在LB液体培养基中用异丙基硫代-β-D-半乳糖苷(IPTG)诱导表达,SDS-PAGE凝胶电泳显示,重组菌表达的MSTN蛋白是以包涵体的形式表达的;SDS-PAGE凝胶经薄层扫描仪扫描分析,表达的MSTN包涵体蛋白占菌体不溶性蛋白含量的27.9%,表达的MSTN分子质量为41.4513ku。因为所构建的表达载体中含六聚组氨酸标签,用His-trap亲和柱进行纯化后,纯度可达92.5%。 相似文献
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Dietary amino acids imbalance will result in stunted broiler performance and deteriorated meat quality, which are involved in various biochemical cycles in vivo. In this study, the effects of dietary methionine on meat quality and methylation of myostatin exon 1 were investigated. Drip loss of the broilers fed with diet of high methionine levels （0.2%） increased from （6.3 ± 0.1）% （control group） to （10.1 ± 1.0）%, and the muscle shearing force increased from （22.8 ± 1.9） N （control group） to （26.3 ±2.3） N. Moreover, many CpG sites were found at the myostatin exon 1 region （nucleotides 2 360-2 540 bp）. To further understand the regulation of broiler myostatin expression, the methylation status of broiler myostatin exon 1 and its mRNA expression were analyzed. At the myostatin exon 1 region where CG enriches （nucleotides 2 360-2 540 bp）, the percentages of methylation were 46 and 84% in low Met and high Met content groups after 55-d feeding, respectively. In skeletal muscle tissues, the exon 1 hypermethylation status of myostatin gene was found to be negatively correlated with the gene expression. These results suggested that methylation of this gene is a dynamic process, which plays a dominant role in regulating gene expression for development of individuals. 相似文献
85.
Boman IA Klemetsdal G Nafstad O Blichfeldt T Våge DI 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2011,128(1):52-55
In this study we show that selection based on progeny testing is able to induce a rapid change in allele frequency, even when a fairly broad and balanced breeding goal is applied. The myostatin 3'-UTR mutation (c.*1232G>A) previously found to affect muscularity in Texel sheep is also present in the Norwegian White Sheep population. By genotyping the rams used for artificial insemination (born in1977-2006), a rapid increase in the c.*1232G>A allele frequency was observed, from 0.31 in 1990 to 0.82 in 2006. The major increase was observed after BLUP-based breeding values and the EUROP classification system for carcass quality was implemented in 1991 and 1996, respectively. The MSTN frameshift mutation c.960delG, recently identified in this population, did not show a similar increase in allele frequency during the same period, in spite that it has a strong desirable effect on meat and fat traits. The results also illustrate that unwanted side effects can rapidly be introduced into a population using an efficient breeding scheme. A system for monitoring changes in phenotypic traits additional to those under selection is therefore recommended to identify possible side effects at an early stage. 相似文献
86.
Molecular Cloning of Myostatin Partial cDNA of Beijing Duck and Its Expression in Breast Muscle 总被引：2，自引：0，他引：2
WANG Yong-sheng HOU Shui-sheng HUANG Wei KANG Jun-mei 《中国农业科学(英文版)》2006,5(6):468-472
In this experiment, 500 bp cDNA of myostatin gene was cloned from a Beijing duck＇s breast, The duck myostatin gene was found to have 98, 96, 95, 88, and 87% sequence similarity at the cDNA level with domestic goose, chicken, domestic pigeon, human, and pig, respectively. The predicted amino acid sequence has an overall similarity with a comparable region of turkey 99%, domestic goose 98%, and chicken 99%. Conserved domains of deduced amino acids showed that it belonged to the TGF-beta family. Myostatin expression in breast muscle was higher at 28, 35, and 42 days than at 7, 14, and 21 days. The pattern of myostatin expression was closely parallel to the trend of breast muscle growth, suggesting that myostatin might play an important role in breast muscle development. It was possible to postulate that myostatin may be a major determinant of muscle mass in breast muscle, as shown in other species. 相似文献
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Susumu MUROYA Kouichi WATANABE Shinichiro HAYASHI Masato MIYAKE Shigeru KONASHI Youichi SATO Manabu TAKAHASHI Shigeki KAWAHATA Yoshisato YOSHIKAWA Hisashi ASO Koichi CHIKUNI Takahiro YAMAGUCHI 《Animal Science Journal》2009,80(6):678-685
To clarify muscle type‐specific effect of myostatin on myogenic regulatory factors (MRFs), we examined mRNA expression of MRFs in five skeletal muscles of normal (NM) and myostatin‐deficient double‐muscled (DM) adult Japanese Shorthorn cattle by quantitative reverse‐transcribed PCR. Among the four MRFs, namely, Myf5, MyoD, myogenin, and MRF4, MyoD expression was different among the muscles of the DM cattle (P < 0.01) but not of the NM cattle. Meanwhile, MyoD expression was significantly elevated only in masseter (MS) muscle in the DM cattle due to the myostatin deficiency (P < 0.05). Myf5 and MRF4 expression in semitendinosus (ST) was higher in the DM than in the NM cattle (P < 0.05). According to analysis of myosin heavy chain (MyHC) isoform expression, more MyHC‐2x and ‐2a and less ‐slow isoforms were expressed in the longissimus and ST muscles compared to the MS muscle in both cattle (P < 0.05), but no significant difference in MyHC expression was observed between the NM and DM cattle. Taken together, myostatin has influences on Myf5 and MRF4 expression in faster‐type muscles and on MyoD expression in slower‐type muscles, suggesting a possible muscle type‐specific effect of myostatin in skeletal muscle growth and maintenance. 相似文献
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采用PCR-RFLP技术检测连续2个世代边鸡群体肌肉生长抑制素基因外显子1的多态性,分析了多态位点对边鸡生长性状的遗传效应。结果表明,边鸡MSTN基因的C.234位点存在多态性,在外显子1编码区的234bp处发生G→A的碱基突变,产生了GG、GA和AA 3种基因型。关联分析表明,无论是一世代还是二世代的边鸡群体,在6~16周龄时,AA和GA基因型边鸡的体质量均显著或极显著的高于GG基因型（P〈0.05或P〈0.01）。结果提示,MSTN基因外显子1的C.234位点突变对边鸡的生长性状具有显著的遗传效应,初步推断该位点可用于边鸡生长性状的标记辅助选择。 相似文献
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Naofumi Yasaka Keisuke Suzuki Yasuhiro Kishioka Jun‐ichi Wakamatsu Takanori Nishimura 《Animal Science Journal》2013,84(9):663-668
Myostatin is a growth and differentiation factor and acts as a negative regulator of skeletal muscle mass. Although the mechanism whereby myostatin controls muscle cell growth is mostly clarified, the regulation of myostatin activity after its secretion into the extracellular matrix (ECM) is still unclear. In the present study, we investigated the interaction between laminin and myostatin and the effect of laminin on myostatin signaling in vitro. The surface plasmon resonance assay showed that laminin bound to mature myostatin and activin receptor type IIB (ActRIIB), but did not bind to latency‐associated protein, which remains non‐covalently linked to mature myostatin. Furthermore, kinetic analysis demonstrated that the affinity of mature myostatin for laminin was similar to that for ActRIIB. Next, we examined the action of laminin on the myostatin signaling pathway using a conventional reporter assay. The luciferase activity of myostatin‐treated cells was repressed significantly (P < 0.05) by coincubation of laminin. These results suggest that laminin has a potential to regulate myostatin activity through binding to mature myostatin and/or its receptor ActRIIB. 相似文献