质量控制图在水产饲料氨基酸分析中的应用   总被引：1，自引：0，他引：1  

杜雪莉  宋军  张凤枰  李应东  刘耀敏 《淡水渔业》2015,(4)

通过对实验室自制标准物料-豆粕样品中氨基酸含量的多次测定,建立X-S质量控制图,开展水产饲料氨基酸分析日常质量控制。试验按照GB/T 18246-2000,通过微波水解-离子交换-柱后衍生-紫外检测法对豆粕样品氨基酸含量进行连续测定,并以FAPAS奶粉标准物质验证,利用偏度-峰度检验法对离群值进行判断,建立赖氨酸、甘氨酸、氨基酸总量质量控制图。结果显示,质量控制图中豆粕质控样赖氨酸、甘氨酸、氨基酸总量测定的RSD值均小于4%,FAPAS奶粉标准物质测定值落在参考范围内,建立的质量控制图能对日常氨基酸检测结果进行有效监控。结果表明,所建立氨基酸质量控制图直观有效,能对过程存在的异常因素起到预警作用,并可节约成本。  相似文献   

猪胆囊中沙门菌L型的分离培养与invA基因检测     

佘晓玲  王涛  丁文静  吴文娟  潘耀振  汤可立  王丹霓  王和 《动物医学进展》2015,(3):25-28

为了解猪胆囊中沙门菌L型携带情况,在贵阳市屠宰场采集970例健康生猪的胆囊组织与胆汁标本,用常规细菌学方法和非高渗分离培养法分离沙门菌及其细菌L型,用PCR和核酸序列分析方法对稳定L型纯培养物进行沙门菌的invA基因检测。结果显示,970例生猪胆囊标本未检出沙门菌细菌型,细菌L型检出率为8.25%;80例细菌L型分离物中有50例invA检测阳性,占5.15%;占细菌L型阳性分离物62.50%。研究结果为生猪胆囊沙门菌L型感染的流行病学及其检查提供了依据。  相似文献   

凝胶渗透色谱净化-气相色谱法同时测定饲料中4种抗氧化剂   总被引：1，自引：1，他引：0  

杨晓凤 《中国饲料》2011,(5)

本文建立了凝胶渗透色谱净化(GPC)-气相色谱法(GC)同时测定饲料中2,6-二叔丁基对甲酚(BHT)、叔丁基羟基茴香醚(BHA)、叔丁基对苯二酚(TBHQ)和乙氧喹(EQ)4种抗氧化剂方法。4种抗氧化剂在10～400mg/L时与峰面积有良好的线性关系(r2>0.9999),检出限(3S/N)为0.37～0.46 mg/kg,添加回收率为82.7%～98.8%,相对标准偏差(n=5)为1.7%～4.3%。  相似文献   

Feeding native laying hens diets containing palm kernel meal with or without enzyme supplementations: 1. Feed conversion ratio and egg production     

《The Journal of Applied Poultry Research》2011,20(1):40-49

  相似文献   

莱芜猪胴体切块质量预判     

张伟力 《养猪》2014,(2):63-64

本文以莱芜猪肉质育种为例，介绍了在屠宰线上对胴体的肉色、大理石纹、特别是肌内脂肪进行主观评定预测的简单易行的方法，其结果可用于肉质选种选育。  相似文献   

Feed preference and growth response of juvenile Litopenaeus vannamei to supplementation of marine chemoattractants in a fishmeal‐challenged diet     

Alberto J. P. Nunes  Hassan Sabry‐Neto  Severino Oliveira‐Neto  Lena Burri 《Journal of the World Aquaculture Society》2019,50(6):1048-1063

This study compared the feed preference and growth response of Litopenaeus vannamei to chemoattractants. A diet with 3% fishmeal was supplemented with either 3% salmon meal (POS), 3% soy protein concentrate (NEG), 3% krill meal (KRM), 3% squid meal (SQM), 3% shrimp head meal (SHM), 3% shrimp meal (SM), 3% squid liver meal (SLM), or 5% liquid sardine hydrolysate (SAH). Shrimp with a body weight (BW) of 0.99 ± 0.08 g were stocked at 100 animals/m² in 56 tanks of 1 m³ and fed 10 times daily for 74 days. Feed preference was evaluated by feeding shrimp of 10.87 ± 1.82 g in excess twice a day for 10 days in two separate feeding trays allocated in 50 tanks of 0.5 m³. Survival reached 93.3 ± 5.80% and was unaffected by supplementation. Final BW was the highest for shrimp fed the KRM‐supplemented diet (11.97 ± 0.93 g), followed by POS (11.11 ± 0.77 g) and SQM (11.01 ± 1.17 g). Diets SHM, SM, SLM, and NEG showed a lower shrimp BW than POS, but were not statistically different among them. Shrimp fed the SAH diet achieved the lowest BW (10.06 ± 1.02 g). The highest gained yield was obtained with diets KRM and POS. No statistical difference was observed in shrimp yield among other diets. The lowest feed conversion ratio (FCR) was achieved with shrimp fed KRM (1.31 ± 0.05) when compared to diets SHM (1.47 ± 0.05), SAH (1.47 ± 0.07), and SLM (1.45 ± 0.17). Two‐by‐two comparisons indicated that shrimp preferred SHM and KRM, except when these were compared to SQM and SLM. No difference in feed preference was found between diets with SQM and SLM. SAH was the least preferred raw material in all comparisons. Results indicated that KRM acts as a powerful feeding effector and growth enhancer in fishmeal‐challenged diets for whiteleg shrimp. A dietary supplementation with 3% KRM is more effective than the same dose of any other chemoattractant evaluated.  相似文献   

猪hnRNPUL1基因克隆及变异剪接体鉴定     

宋艳芳  张彩霞  杜芳芳  马骏杰  刘东宇  刘娣  杨秀芹 《畜牧兽医学报》2020,51(3):443-451

旨在克隆民猪hnRNPUL1基因全长编码区（complete coding sequence，CDS）序列并进行可变剪接分析，研究其组织表达和亚细胞定位情况。本研究以3头3月龄民猪为试验材料，利用RT-PCR技术克隆hnRNPUL1基因CDS及可变剪接体，应用qRT-PCR检测其在心、肝、脾、肺、肾、胃、大肠、小肠、背肌、腿肌等组织中的表达情况，同时，在生物信息学预测的基础上，通过融合表达绿色荧光蛋白的方法鉴定核定位序列。研究结果表明，猪hnRNPUL1基因（GenBank accession No.：MN399154）CDS全长2 580 bp，预期编码的多肽链存在着hnRNPs家族的特征性结构域——SAP、SPRY和AAA；猪hnRNPUL1普遍表达于所检测的各组织中，其中在脾脏中表达量最高，在腿肌中表达量最低；核定位序列（NLS）位于多肽链的133~430 aa处，与生物学信息学预测的结果存在着偏差；同时获得了2个变异剪接体和11种可变剪接片段。本研究结果对进一步揭示猪hnRNPUL1基因功能及转录调控机制提供了基础。  相似文献   

互联网+环境下饲料品牌的策划和实践     

王雯 《中国饲料》2021,(2):94-97

随着市场环境的不断发展变化,消费者越来越重视产品的品牌效益,品牌建设已成为当前饲料企业市场竞争中取胜的重要法宝,饲料企业可通过品牌策划与实践提升产品的知名度,加强企业自身的商业信誉,从而提升饲料产品的销售份额,有效扩大消费群体,为企业的长远发展提供广阔的潜力。因此,饲料企业要重视互联网+环境下品牌的策划与实践,通过优化策划方案、创新推广路径等方式加强企业品牌建设。  相似文献   

Selecting the hologenome to breed for an improved feed efficiency in pigs—A novel selection index     

Ramona Weishaar  Robin Wellmann  Amelia Camarinha-Silva  Markus Rodehutscord  Jörn Bennewitz 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2020,137(1):14-22

Most traits in animal breeding, including feed efficiency traits in pigs, are affected by many genes with small effect and have a moderately high heritability between 0.1 and 0.5, which enables efficient selection. Since the microbiota composition in the gastrointestinal tract is also partly heritable and was shown to have a substantial effect on feed efficiency, the host genes affect the phenotype not only directly by altering metabolic pathways, but also indirectly by changing the microbiota composition. The effect of the microbiota composition on the breeding value of an animal is the conditional expectation of its breeding value, given the vector with microbiota frequencies, that is The breeding value of an animal can therefore be decomposed into a heritable contribution that arises from an altered microbiota composition and a heritable contribution that arises from altered metabolic pathways within the animal, so Instead of selecting for breeding value , an index comprising the two components and with appropriate weights, that is , can be used. The present study shows how this breeding strategy can be applied in pig genomic selection breeding scheme for two feed efficiency traits and daily gain.  相似文献   

基层常见猪传染性腹泻病的临床诊断与防控对策     

杨小金  殷玉武 《金陵科技学院学报》2014,(3)

猪传染性腹泻病是猪的常见多发病。基于受基层单位病原检测技术的条件限制,一旦发生传染性腹泻病,基层兽医通常依据临床症状做出初步诊断与治疗,如果临床经验稍有不足,误诊、误治或延误诊治的现象时有发生。为此梳理出了常见的12种猪传染性腹泻病临床诊断要点与综合防治措施,以防止其重大疾病的发生。  相似文献