酱油渣不溶性膳食纤维的功能特性及其应用研究     

王忠合 《农产品加工．学刊》2010,(3):28-31,37

研究酱油渣中不溶性膳食纤维的膨胀性、持水能力、持油性、亚硝酸根离子吸附性,以及阳离子交换能力等功能特性,及其在桃酥中的应用。结果表明,酱油渣不溶性膳食纤维具有较好的膨胀性、持水能力、持油性和一定的阳离子交换能力,对亚硝酸根离子的吸附性能随着时间的增加而增大,120min时的吸附性趋于饱和,吸附率高达83.24%,且在酸性条件下的吸附性能更佳。应用实验表明,在桃酥中添加适量的酱油渣不溶性膳食纤维,不仅对桃酥的口感、色泽及组织形态等方面的影响较小,而且其保健功能有很大的提高。  相似文献   

棉铃虫HaHR3基因的克隆及其植物RNA干扰载体的构建     

尹富仕  曾洪梅  张雨良  邱德文  杨秀芬  王立梅 《棉花学报》2010,22(2):157-162

采用RT-PCR方法克隆了棉铃虫蜕皮调节转录因子HaHR3基因,该基因含有1671 bp的完整开放阅读框。序列分析表明,HaHR3与多种昆虫蜕皮调节转录因子高度同源。利用生物信息学方法对HaHR3的结构特征进行分析发现,HaHR3具有蜕皮调节转录因子超家族的典型特征,包括两个锌指结构和一个DNA结合结构域,不存在信号肽序列和N糖基化位点。选择HaHR3基因的部分片段构建了RNAi中间载体pRNAi1017-HaHR3sa,再将HaHR3正反向序列亚克隆至植物表达载体pCAMBIA2300-35S-OCS,成功构建了由35s启动子调控的HaHR3基因的正反向RNA干扰载体pCAM-RNAi-HaHR3。这一载体的成功构建为下一步通过植物介导的RNA干扰技术防治棉铃虫打下了坚实的基础。  相似文献   

基于注意力机制的时空卷积数控机床热误差模型研究     

杜柳青  李仁杰  余永维 《农业机械学报》2021,52(5):404-411

为了提高数控机床热误差模型的精度与泛化性,提出了基于注意力机制的长短时记忆卷积神经网络(Long short term memory convolutional neural network based on attention mechanism, AM-CNN-LSTM)热误差模型。利用卷积神经网络提取高维数据空间状态特征的能力和长短时记忆网络提取长时间序列状态特征的能力,构建具有2个支路的热误差模型,分别提取特征后输入到注意力机制中进行特征重要性重构,建立原始数据与热误差的特征映射,最后通过全连接层进行热误差预测。采用G460L型数控机床进行实验数据采集,将不同季节采集到的温度数据和热误差作为模型输入,采用循环学习率与正则化优化方法对模型进行训练。与LSTM、ConvLSTM和CNN-LSTM热误差模型对比,结果表明,AM-CNN-LSTM模型对特征还原能力最强,残差波动范围最小,其残差范围较最大值下降62.09%,模型预测精度在2.4μm以内。  相似文献   

Cloning and characterization of transcripts differentially expressed in the pulp of ripening papaya     

João Paulo Fabi  Franco Maria LajoloJoão Roberto Oliveira do Nascimento 《Scientia Horticulturae》2009

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利用DAD1反义片段转化创建菜薹可调控雄性不育材料   总被引：1，自引：0，他引：1  

陈国菊  徐飞  雷建军  曹必好 《园艺学报》2009,36(5):685-692

 菜薹因为没有好的雄性不育材料或自交不亲和系,至今尚无一代杂种用于生产,根据拟南芥及白菜型油菜的花药不开裂基因DAD1的保守序列设计引物,扩增菜薹的DAD1基因片段（DAD1F）,构建反义DAD1F植物表达载体,用农杆菌介导法转化菜薹,对转基因植株进行分子检测,鉴定其雄性不育性并进行育性恢复试验.克隆得到的菜薹的DAD1基因片段大小为678 bp,命名为BrcpDAD1F,其序列与拟南芥和白菜型油菜的DAD1高度同源,同源率分别为88％和99％;共得到了12株转基因植株,有6株在转录水平上得到表达,表现为雄性不育,花器官畸形,花粉活力低,萌发率不到10％,且开花后不能结角果或结空角果,或者得到极少种子但种子不萌发;用对照的花粉给转基因植株授粉可使其正常结实.以500 μmol·L^-1茉莉酸甲酯处理可使其雄性不育得到恢复,花粉可以在柱头和培养基上萌发,具有受精能力。T₁代可育株与不育株的比例都呈1:3分离, T₂代不同株系的育性分离比例不同,有些株系继续呈1:3的分离,有些株系全是可育株或全是不育株,说明反义抑制呈单基因稳定遗传。  相似文献   

高湿地区板栗林下栽培模式的研究     

段显德  董荣春 《北方园艺》2009,(4)

对板栗林下栽培不同的经济作物进行研究.结果表明:板栗园套种短梗五加具有投资少,收益高,见效快的特点,并且具有保持水土,改善板栗园生态环境的功效.最高每667 m2较单一栽培多收入1 165.75元,是辽东板粟产业继续发展壮大的较理想模式.  相似文献   

锦鲤保护性组织RNA提取及引物扩增研究   总被引：1，自引：0，他引：1  

王亚军  刘必谦  吴立山  洪松柏 《水生态学杂志》2008,29(2):116-119

从养殖锦鲤各种组织中提取完整的RNA, 是分子生物学研究锦鲤应激表达、体色变异、生长发育等功能基因表达的关键实验。在不伤害养殖锦鲤个体健康的情况下, 取用鱼体的鳍条、鳞片、鳃丝、血液4种保护性组织,可以最大程度地减少对珍贵锦鲤个体的损伤, 并达到研究相关基因表达的目的。本研究利用TR izo l法提取RNA, 获得的各样品RNA条带完整、纯度高。结果表明, 4 种保护性组织提取的RNA 经过逆转录反应, 得到了高质量的CDNA。内标基因( B- actin)、热激蛋白基因( hsp70)、金属硫蛋白基因(MT)扩增得到清晰的条带, 通过NCB I数据上的BLAST比对证明, 各序列的同源性在95%以上。利用本研究的方法, 可以充分保护养殖锦鲤个体的完好性, 用于进一步的分子生物学研究。  相似文献   

Regulation of osteopontin expression by its antisense RNA in renal epithelial cells     

CHEN Yong-xiong  LI Jin-hua  YU Xue-qing  HUANG Ling-hong  CHEN Wei-ying  LU Jun  FAN Chong-lun  YIN Pei-da 《园艺学报》2000,16(11):1153-1158

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Sinomenine reduces MPP⁺-induced damage in SK-N-SH cells via ANRIL/miR-626 signaling pathway     

HUO Ying-hao  WANG Jin  YIN Li-xin 《园艺学报》2000,36(8):1404-1413

AIM To investigate the effect of sinomenine (SN) on the damage of human neuroblastoma SK-N-SH cells induced by 1-methyl-4-4 phenylpyridine (MPP⁺) and its mechanism for exploring the pathogenesis of Parkinson disease. METHODS SN was used to treat MPP⁺-induced SK-N-SH cells. The levels of malondialdehyde (MDA) and glutathione (GSH) in cell culture supernatants were measured by ELISA. The apoptosis was analyzed by flow cytometry. The protein expression levels of Bcl-2 and Bax were determined by Western blot. The expression levels of long noncoding RNA ANRIL and microRNA-626 (miR-626) were detected by RT-qPCR. Dual-luciferase reporter assay was used to evaluate the relationship between ANRIL and miR-626. After ANRIL small interfering RNA was transfected into SK-N-SH cells, the effects of ANRIL expression knock-down on MPP⁺-induced SK-N-SH cell apoptosis, the protein expression levels of Bcl-2 and Bax, and the levels of MDA and GSH in cell culture supernatants were examined. RESULTS After treatment with MPP⁺, the apoptotic rate, Bax protein level and ANRIL expression in SK-N-SH cells were increased (P<0.05), and the Bcl-2 protein level and miR-626 expression were decreased (P<0.05). The level of MDA in cell culture supernatants was increased (P<0.05), and the level of GSH was decreased (P<0.05). After SN treatment or ANRIL expression knock-down, decreased apoptotic rate, Bax protein level and ANRIL expression (P<0.05), and increased Bcl-2 protein level and miR-626 expression in MPP⁺-induced SK-N-SH cells were observed (P<0.05). The level of MDA in the cell culture supernatants was decreased (P<0.05), and the level of GSH was increased (P<0.05). CONCLUSION SN attenuates MPP⁺-induced damage in SK-N-SH cells by regulating ANRIL/miR-626 signaling pathway.  相似文献   

Progress in roles of MNX1 antisense RNA 1 in malignant tumor     

GUAN Cang-hai  ZHAO Yu-qiao  Guo Liang  CHEN Yu-Zhu  WANG Wei-Na  JIANG Xing-ming 《园艺学报》2000,36(10):1908-1912

MNX1 antisense RNA 1 （MNX1-AS1） is a newly discovered long non-coding RNA （lncRNA）, which is highly dysregulated in various carcinomas and its expression level is closely related to the overall survival and prognosis of patients. MNX1-AS1 regulates the occurrence and development of carcinomas by endogenous competitive adsorption of miRNA, regulating cell cycle, inducing epithelial mesenchymal transformation and activating multiple signaling pathways. The in-depth study of the carcinogenesis of MNX1-AS1 is useful for the early diagnosis, targeted therapy and prognostic assessment of relevant carcinomas. This article reviews the roles of MNX1-AS1 in malignant tumor.  相似文献