东北虎源等孢球虫在猫体内的卵囊排出规律观察     

张靖伟  路义鑫  张子群  韩彩霞  王守育  宋铭忻 《中国预防兽医学报》2009,31(11)

为研究猫体内的卵囊排出规律,本研究分离的东北虎源等孢球虫纯种卵囊,在猫体内多次传代后获得充足的卵囊.对猫进行人工感染30、10~3和10~4个孢子化卵囊,观察猫的卵囊排出规律,包括潜隐期、显露期、排卵囊高峰期及每克粪便中卵囊数(OPG 值).通过对比,验证了潜隐期、显露期、OPG值与感染剂量存在相关关系,即感染剂量越小,潜隐期越长,显露期越长,OPG值越小,反之亦然.  相似文献   

牛源环孢子虫的形态学特征观察     

肖淑敏李国清  李韦华周荣琼 《中国兽医科技》2006,36(8):639-642

对首次在乳牛粪便中发现的疑似环孢子虫卵囊的形状、大小、抗酸染色和孢子化等形态学特征进行了观察，探讨了该原虫的纯化方法。结果显示，牛源环孢子虫卵囊呈球形，直径6～10μm。直接涂片时可见卵囊内含折光性较强的成团小球体，呈桑椹胚状，淡绿色。碘液染色不着色。经改良抗酸染色后，卵囊着色变化较大，呈淡红色、深红色或不着色。新排出的未孢子化卵囊培养后可观察到孢子化的卵囊，内含2个孢子囊，每个孢子囊内含有2个折光性较强的子孢子。这些特征与人或其他动物的环孢子虫一致。环孢子虫感染阳性的粪便经Sheather＇s蔗糖漂浮法浓集后，再经蔗糖密度梯度离心，可以得到较为纯净的卵囊。  相似文献   

四项球虫抗药性判定指标的准确性评价   总被引：5，自引：0，他引：5  

陈兆国  黄兵  赵其平  史天卫  吴薛忠 《中国兽医寄生虫病》2001,9(4):38-42

从指标各自的特点、评价结果的一致性、其它指标、试验总体结果和发展趋势五个方面，分别对上海地区鸡球虫进行抗药性监测时采用的笼饲试验的4项判定指标进行了评述，认为在当前条件下，用抗球虫指数（ACI）、病变记分减少率（RLS）、相对卵囊产量（ROP）、最适抗球虫活性百分率（POAA）4项指标来综合评定球虫抗药性的方法是有效的、可靠的。比较而言，RPO、RLS的错判率较高，RLS的漏判率较高；POAA错、漏判率的较低，优于ROP、RLS。而ACI错、漏判率均最低，准确性最好。  相似文献   

柔嫩艾美耳球虫大配子发育和卵囊的超微结构     

安健  汪明  孔繁瑶  殷佩云 《中国兽医学报》2001,21(6):559-561

利用透射电镜对柔嫩艾美耳球虫大配子发育和卵囊的超微结构进行了观察。结果表明，大配子体和小配子体寄生于相邻的宿主细胞内，相伴产生。大配子体的发育、大配子的形成、受精及卵囊形成均于带虫空泡内完成。大配子体系由末代裂殖子侵入肠上皮细胞后，缩短、长大、变圆而形成，大配子体和大配子外被单位膜，细胞核位于大配子体中央，早期大配子体的细胞核有1个明显的核仁，细胞质内逐渐形成大量成囊体1、成囊体2、大量支链淀粉和脂肪体，成囊体1比成囊体2形成的早。卵囊壁有5'层，内有大量支链淀粉和指肪体。含有大配子体、大配子和合子的带虫空泡内有丰富的泡内小管，它们是大配子体、大配子和合子获得营养物质和排出代谢废物的途径。  相似文献   

Differential Proteomic Analysis between Sporulated Oocyst and Unsporulated Oocyst of Cystoisospora suis     

YANG Shoushen  SUN Xiaoshuang  QIU Yunfei  YANG Fei  TANG Jie  LIAN Wenjian  HUANG Cuiqin  CHIU Hungchuan 《畜牧兽医学报》1956,51(9):2216-2226

The differentially expressed proteins between sporulated oocyst and unsporulated oocyst of Cystoisospora suis strains were screened and compared. In this study, the C. suis infection model of piglets were established and a quantitative proteomic analysis between sporulated oocyst and unsporulated oocyst of C. suis were conducted by iTRAQ labeling and LC-MS/MS. Part of the iTRAQ results were validated by qRT-PCR. Total 174 proteins were identified, among which 40 proteins were differentially expressed, 38 proteins were upregulated and 2 proteins were downregulated. The differentially expressed proteins were mainly involved in metabolism, antibiotics biosynthesis, secondary metabolites biosynthesis and glycolysis/gluconeogenesis pathways. The results of this study provide reference for elucidating the developmental mechanism of C. suis sporulated oocyst and facilitated discovering a new biomarker for C. suis.  相似文献   

盐酸氟乙基硫胺素的药效学研究     

覃宗华 《中国兽药杂志》2014,48(8):32-35

采用人工感染艾美耳球虫模型来评价抗球虫药盐酸氟乙基硫胺素的治疗效果,以期为临床用药提供依据。试验选取鸡240只,随机分为8组,每组30只,其中2组分别为模型组（感染不给药组）和空白对照组,另外6组分别为氨丙啉组、尼卡巴嗪组、马杜拉霉素组及盐酸氟乙基硫胺素组（高、中、低三个剂量）,4种抗球虫药物进行治疗。除空白对照组外,其余各组动物接种球虫孢子化卵囊2.5×105个/只。连续给药5 d,在第7天将240只鸡全部扑杀剖检,根据存活率、相对增重率、卵囊值、病变值计算抗球虫指数（ACI）,进而判定药物的疗效。结果表明,125 mg/kg 氨丙啉组ACI为191.2、125 mg/kg 尼卡巴嗪组ACI为192.9、5 mg/kg马杜拉霉素组ACI为186.7,均大于180,为高效抗球虫药;125 mg/kg 盐酸氟乙基硫胺素组ACI为201.6,为高效抗球虫药。盐酸氟乙基硫胺素的抗球虫效果优于氨丙啉、马杜拉霉素及尼卡巴嗪。  相似文献   

牛隐孢子虫病的研究──治疗试验与田间试验     

张友三  薛登民  杨新民 《西北农林科技大学学报(自然科学版)》1995,23(5):74-77

用止泻粉１２５～１４０ｍｇ／ｋｇ体重，给５～３０日龄隐孢子虫病犊牛３０头、与细菌混合感染的隐孢子虫病成年牛８７头、田间试验病犊２９５头口服．结果表明，临床治愈率分别为９３．３３％，９４．２５％和９４．２４％；粪便中隐孢子虫卵囊数量迅速显著下降，并维持在低水平，有４０．０％～５３．３％的牛排卵囊转阴。用青蒿琥酯５ｍｇ／ｋｇ体重，给５～３０日龄有病犊牛３０头口服，有效率为５２．０％，卵囊减少不明显。  相似文献   

猪囊等孢球虫孢子化与未孢子化卵囊的差异表达蛋白质组学分析     

杨守深  孙晓双  邱云飞  杨飞  唐杰  连文健  黄翠琴  邱泓铨 《畜牧兽医学报》2020,51(9):2216-2226

通过比较猪囊等孢球虫孢子化卵囊与未孢子化卵囊的差异表达蛋白质,以期从蛋白质组学角度研究其发育机制,并为此类球虫的发育机制及该病的免疫预防或化学防治找到新的“关键”分子奠定基础。首先建立哺乳仔猪的猪囊等孢球虫感染模型,然后运用iTRAQ技术,结合LC-MS/MS分析差异表达蛋白质,并采用荧光定量PCR验证iTRAQ数据。结果共鉴定出174个蛋白,差异表达蛋白40个,其中38个蛋白上调表达,2个蛋白下调表达,差异蛋白主要涉及代谢、抗生素的生物合成、次生代谢产物的生物合成和糖酵解/糖异生过程等通路。研究结果为阐明猪囊等孢球虫孢子化发育机制以及发现新的生物标志物提供参考。  相似文献   

实验性雏鸡隐孢子虫病治疗试验     

曹小明  刘毅  李健夫  李道解  傅童生 《湖南农业大学学报(自然科学版)》1994,(3)

评价了交沙霉素、大蒜素、甲硝唑、复方新诺明等４种药物对实验性雏鸡隐孢子虫病的治疗效果．雏鸡感染后第９ｄ起，分组在饲料中添加交沙霉素（８．０ｇ／ｋｇ）、大蒜素（６ＯＯｍｇ／ｋｇ），甲硝唑（４．０ｇ／ｋｇ），复方新诺明（８．６ｇ／ｋｇ），连续５ｄ任鸡采食，结果试验组鸡粪中卵囊数明显低于对照鸡，病鸡症状和眼观病变得到缓解，表明这４种药物对雏鸡实验性隐孢子虫病有一定治疗作用．  相似文献   

The prevalence and intensity of infection withEimeria species in sheep in Nyandarua district of Kenya     

N. Maingi  W. K. Munyua 《Veterinary research communications》1994,18(1):19-25

The prevalence and numbers of coccidian oocysts in faecal samples from young (less than 6 months old), immature (6–12 months old) and adult (over 12 months old) sheep on 15 farms in Nyandarua district were studied during the dry and wet seasons. The species ofEimeria occuring in these sheep were also identified. The proportion of animals shedding coccidian oocysts did not vary significantly with season. The prevalence of the oocysts was significantly higher (p<0.05) in young sheep (mean 85.3%) compared to immature (mean 40.2%) and adult sheep (mean 32.15%). OPG counts (oocysts per gram of faeces) were significantly higher (p<0.01) in the young sheep compared to immature and adult sheep during both seasons. Prevalence and OPG did not differ between immature and adult sheep. There was no significant difference in OPG during the wet season (mean 328±997) compared to the dry season (mean 219±773). The sex of the sheep had no significant effect on prevalence or OPG. Eight species ofEimeria were recognized. They (and their prevalence) wereE. bakuensis (ovina) (43.6%),E. ovinoidalis (23.6%),E. ahsata (15.2%),E. intricata (8.27%),E. granulosa (4.8%),E. faurei (2.8%),E. parva (1.06%) andE. pallida (0.67%).Abbreviations OPG oocysts per gram of faeces  相似文献