Ocular involvement of transmissible venereal tumor in a dog     

Boscos CM  Ververidis HN  Tondis DK  Stamou AI  Samartzi FC 《Veterinary ophthalmology》1998,1(2-3):167-170

A case of metastatic transmissible venereal tumor (TVT), with lesions on the penis, conjunctiva, buccal mucosa and skin (lips and trunk), is presented in this case report. The clinical picture is described along with the cytological and histopathological features of the tumor leading to definitive diagnosis of TVT. Possible reasons for the unusual metastatic behavior of TVT and full recovery of the dog after chemotherapy are discussed.  相似文献   

Equine coronavirus induces apoptosis in cultured cells     

Suzuki K  Matsui Y  Miura Y  Sentsui H 《Veterinary microbiology》2008,129(3-4):390-395

Equine coronavirus (ECoV) was first isolated from a diarrheic foal and was found genetically similar to group II coronaviruses. However, its pathological characteristics were not adequately investigated. In our preliminary in vitro investigation, ECoV-induced cell death was observed in bovine kidney-derived MDBK cells. Based on this finding, we investigated whether the ECoV-induced CPE was apoptosis. Following ECoV infection, MDBK cells showed morphological changes such as cell rounding and detachment from the culture surface. Moreover, syncytium formation was observed as the other type of cytopathic effect in ECoV infection. Morphologic and biochemical features of apoptosis, such as nuclear fragmentation and DNA ladder formation, were also detected in ECoV-infected cells. Moreover, as is commonly observed in coronavirus infection in other animals, the activities of effecter caspases – caspase-3/7 – and initiator caspases – caspase-8 and caspase-9 – that are representative factors in the death receptor-mediated apoptotic pathway and mitochondrial apoptotic pathway, respectively, were increased in ECoV-infected MDBK cells. Therefore, it was suggested that ECoV can induce apoptosis in MDBK cells via a caspase-dependent pathway. Apoptotic death of infected cells is detrimental because it causes cell and tissue destruction and inflammatory responses. Although the pathological characteristics of ECoV are largely unknown, apoptosis may be the pathological basis of lesions of the digestive system in ECoV infection.  相似文献   

猪传染性胃肠炎病毒spike蛋白亲和肽的原核表达     

于天飞  董慧莹  谢鹏宇 《西北农林科技大学学报(自然科学版)》2020,48(1):49-56

【目的】原核表达猪传染性胃肠炎病毒(transmissible gastroenteritis virus of swine,TGEV)spike蛋白亲和肽(SQHT),检测其病毒亲和性,为建立TGEV诊断方法奠定理论和物质基础。【方法】人工合成TGEV spike蛋白亲和肽基因,亚克隆后将该基因分别插入至原核表达载体pET-32a和pGEX-6p-1中,构建重组质粒pET-32a-SQHT和pGEX-6p-SQHT,对重组质粒进行BamHⅠ单酶切和BamHⅠ/XhoⅠ双酶切及测序鉴定。将重组质粒分别转化至大肠杆菌Rosetta(DE3),用IPTG进行诱导表达,对其表达产物的生物学活性进行检测。【结果】亚克隆获得了150 bp的TGEV spike蛋白亲和肽基因。成功构建了重组质粒pET-32a-SQHT和pGEX-6p-SQHT,并诱导表达出重组蛋白TRX-SQHT和GST-SQHT,其分子质量分别为25和31 ku。Western blot分析表明,2种重组蛋白与TGEV病毒粒子具有良好的亲和性;Dot-ELISA分析表明,2种重组蛋白与TGEV病毒粒子的最低结合滴度TCID_(50)为5×10~2 mL~(-1)。特异性试验表明,重组蛋白TRX-SQHT和GST-SQHT均可与TGEV产生特异性结合,而不与猪流行性腹泻病毒(PEDV)和猪轮状病毒(PoRV)结合。【结论】使用原核细胞成功表达了TGEV spike蛋白亲和肽,表达的重组蛋白具有很好的TGEV特异亲和性。  相似文献   

全血SARS-CoV-2特异性抗体检测对2019-冠状病毒病的临床应用价值分析          下载免费PDF全文

罗效梅  王静  张娅  薛成军  杨坤  向利祥  李海军  韩梅  李梅  邓仁麑  罗福龙  胡昌华  陈耀凯 《西南大学学报(自然科学版)》2020,42(3):30-34

为评价SARS-CoV-2特异性IgM和IgG抗体检测在2019-冠状病毒病(corona virus disease 2019, COVID-19)中的临床应用价值.本试验采用回顾性研究方法,选择重庆市公共卫生医疗救治中心2020年2月4-24日门诊和住院患者作为研究对象,其中COVID-19患者101例,排除COVID-19的其他肺部疾病及发热患者54例作为对照组,以RT-PCR检测结果作为金标准,采用胶体金免疫层析法(gold immunochromatography assay, GICA)检测所有研究对象的SARS-CoV-2特异性IgM和IgG抗体,使用诊断特异性、敏感度、诊断准确度等指标对诊断价值进行评价,并分析抗体与疾病病程以及疾病分型的关系,采用χ~2检验对检测结果进行统计学分析.结果表明, SARS-CoV-2特异性IgM/IgG临床诊断敏感度、特异度和诊断准确度分别为92.1%, 90.7%和91.6%,与RT-PCR方法相比,经过χ~2检验,二者差异无统计学意义(p0.05). SARS-CoV-2特异性IgM和IgG抗体阳性率与病程的关系经过χ~2检验,不同发病天数各组间差异有统计学意义(p0.05).不同疾病分型的SARS-CoV-2特异性IgM和IgG抗体阳性率经过χ~2检验,差异无统计学意义(p0.05).总之, SARS-CoV-2特异性IgG/gM抗体的检测可用于COVID-19感染的诊断,但在疾病早期可能存在漏检的风险.  相似文献   

犬冠状病毒病的免疫预防研究进展   总被引：1，自引：0，他引：1  

孔庆波 《动物医学进展》2007,28(12):72-76

犬冠状病毒病是由犬冠状病毒引起的以胃肠炎为主的一种高度接触性传染病,对幼犬危害尤其严重,发病率和死亡率都很高,是目前对养犬业危害较大的疾病之一.文章就犬冠状病毒感染的流行病学、发病机理、机体免疫、免疫程序和免疫预防进行了综述,特别是对犬冠状病毒病的免疫类型、疫苗种类以及免疫预防存在的问题进行了详细阐述.  相似文献   

Studies on Mucosal Immunity Induced by Transmissible Gastroenteritis Virus Nucleocapsid Protein Recombinant Lactobacillus casei in Mice and Sow     

WANG Gui-hua  HOU Xi-lin  YU Li-yun  LIU Jian-kui  WEI Chun-hua 《中国农业科学(英文版)》2009,8(2):231-237

Mucosal immunity plays an important role in protecting pigs against transmissible gastroenteritis virus （TGEV） infection. To elicit mucosal immune response against TGEV, we developed a surface antigen display system using the poly-γ- glutamate synthetase A （pgsA） protein of Bacillus subtilis as an anchoring matrix to express recombinant fusion proteins of pgsA and nucleocapsid protein of TGEV in Lactobacillus casei. Surface location of fusion protein was verified by ELISA and indirect immunofluorescence test. Oral and intranasal inoculations of pregnant sow and mice with recombinant L. casei resulted in high levels of serum immunoglobulin G （IgG） and secretory immunoglobulin A （slgA） against recombinant N protein as demonstrated by ELISA. More importantly, the level of specific slgA in colostrum significantly increased compared with that of IgG. The serum IgG levels of the piglets increased after suckling colostrum produced by sows was previously inoculated with recombinant L. casei. These results indicate that immunization with recombinant L. casei expressing TGEV N protein on its surface elicited high levels of specific slgA and circulating IgG against TGEV N protein.  相似文献   

猪传染性胃肠炎病毒S蛋白主要抗原位点在昆虫细胞中的表达     

李菁  何孔旺  杨倩 《南京农业大学学报》2009,32(1)

用RT-PCR方法扩增届猪传染性胃肠炎病毒S基因主要抗原位点A、D,将其亚克隆至供体质粒pFastBacl,得到重组转移质粒pFastBael-TS1,转化到Escherichia coli DH5α中,提取阳性克隆质粒再转化到含穿梭载体bacmid的E.coliDHl0Bac中,发生转座作用,经蓝白斑筛选得到重组穿梭载体Bacmid-TS1,经PCR鉴定后再转染Sf9昆虫细胞,经空斑筛选得到纯化的重组病毒rAcV-Bac-FS1,经1FA、SDS-PAGE和Western-blot鉴定,重组杆状病毒TS1蛋白(相对分子质量约43 000) 在Sf9昆虫细胞中得到表达.  相似文献   

严重急性呼吸道综合征(SARS)病毒基因组比较分析与进化研究     

马相如  郭爱珍  肖少波  金梅林  陈焕春 《华中农业大学学报》2003,22(6):523-528

“严重急性呼吸道综合征”(SARS)由于危害严重，引起人们的高度重视，在各国科研人员努力下，目前已完成了十几个毒株的分离与全基因组测序工作。本研究对世界各地分离的SARS毒株的基因组全序列进行比较，发现不同毒株的同源性在99．8％以上，SARS病毒的遗传稳定性较高；将SARS病毒与其它冠状病毒基因组以及编码蛋白质序列进行比较分析，证实SARS病毒是一种新的冠状病毒，可能进化起源较早，并发现SARS病毒和牛冠状病毒同源程度最高。SARS病毒orflab、orfla、S、M、N编码区氨基酸序列和牛冠状病毒同源程度最高，鼠肝炎病毒次之。  相似文献   

牛冠状病毒的血清流行病学调查   总被引：4，自引：0，他引：4  

姚火春  杜念兴  徐为燕  陆承平 《南京农业大学学报》1990,(2)

用血凝抑制试验检测了我国奶牛、黄牛、水牛、牦牛、绵羊、山羊、猪和人共1733份血清中的牛冠状病毒(BCV)抗体,其阳性率分别为80.2%,67%,23.2%,44.3%,5.3%,0%,19%和19.3%。奶牛血清 BCV 抗体有年龄差异,黄牛、水牛、牦牛及人等抗体检出率存在地区性差异。结果表明,BCV 在我国普遍存在,可能为犊牛及成年牛腹泻的重要病原之一。  相似文献   

猪传染性胃肠炎病毒TH-98株S基因的克隆与同源性比较   总被引：2，自引：0，他引：2  

任晓峰  李一经  李广兴  刘宝全 《中国兽医杂志》2002,38(7):3-6

用猪睾丸 (Swine testicle,ST)细胞繁殖并扩增猪传染性胃肠炎病毒国内分离株 TH- 98,根据 Gen Bank中已发表的TGEV S基因 c DNA序列 ,利用 Oligo4.1程序软件设计并合成两对引物 ,进行逆转录聚合酶链式反应 (RT- PCR) ,扩增出2 .3kb和 2 .1kb两个片段 ,分别命名为 Sa与 Sb,先后插入到 p UC18质粒载体上的 Eco RI和 Pst I多克隆位点上 ,构建了重组质粒 p UC- S。根据 TGEV S基因位点和 p UC18的物理图谱 ,用相应的限制性内切酶进行酶切及套式 PCR方法进行鉴定、分析 ,证明克隆的 p UC- S为 TGEV S基因。同时对 p U C- S进行序列测定分析 ,并与来自美国、英国和日本的五个毒株进行核苷酸及编码氨基酸同源性比较 ,证明了 S基因的高度保守性  相似文献