Effect of insemination site and diameter of the pre‐ovulatory follicle on the odds of pregnancy in heifers using sexed or non‐sexed semen          下载免费PDF全文

L Ingenhoff  E Hall  S Ranjbar NI  JK House 《Australian veterinary journal》2017,95(9):317-324

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Uterine torsion diagnosed in a mare at 515 days' gestation     

C. López  J. U. Carmona 《Equine Veterinary Education》2010,22(10):483-486

A pregnant mare with a history of prolonged gestation (～515 days) and suspected diagnosis of fetal mummification was examined. Rectal palpation revealed that the left broad ligament of the uterus was dorsal and medial to the right uterine ligament and it was not possible to observe the cervix during vaginal examination. Transabdominal ultrasound revealed fluid in the uterus, fetal membranes and the uterine walls defined and thickened. Free fluid was not seen in the peritoneal cavity. Laboratory tests (blood cell count and clinical chemistry) were normal. Based on clinical history, physical examination and ultrasound findings, a chronic uterine torsion with fetal death was diagnosed and the mare was subjected to exploratory celiotomy. The uterus was strongly adhered to the peritoneum of the ventral abdominal wall and there were multiple adhesions to the colon. Hysterotomy was performed to remove the fetus and to permit repositioning of the uterus. When the fetus was removed, a large devitalised grey tissue area of the right ventral uterine horn was observed. Multiple adhesions prevented a rescue hysterectomy and euthanasia of the patient was performed. During the necropsy, a 180° cranial cervix clockwise uterine torsion was observed. This rare case of uterine torsion appears to be the most chronic case reported in the equine literature.  相似文献   

双氢睾酮通过调控孕酮、雌二醇和细胞凋亡参与绵羊子宫功能     

丁自强  葛闻博  段宏伟  吕建树  曾建林  王文娟  牛甜  张勇  赵兴绪  胡俊杰 《畜牧兽医学报》2022,53(9):2993-3005

旨在探究双氢睾酮（dihydrotestosterone,DHT）是否通过调节孕酮（progesterone,P₄）、雌二醇（oestrogen,E₂）和细胞凋亡参与影响绵羊子宫功能,以揭示其在绵羊生殖生理中的潜在作用。本试验以1.5岁左右的雌性小尾寒羊为试验动物,检测卵泡期、黄体期和妊娠期子宫中DHT合成酶和雄激素受体（androgen receptor,AR）的表达变化。随后,体外培养绵羊子宫内膜上皮细胞,并用DHT （10^-10~10^-7 mol·L^-1）和AR拮抗剂氟他胺（Flu,10^-8 mol·L^-1）处理（n=3）。通过酶联免疫吸附试验、细胞免疫荧光、蛋白质印迹法和实时荧光定量PCR检测P₄和E₂水平、合成酶和受体表达。此外,还检测经DHT和Flu处理后,绵羊子宫内膜上皮细胞中凋亡因子Bcl-2相关X蛋白（Bcl-2-associated X protein,Bax）、B细胞淋巴瘤蛋白2（B cell lymphoma protein 2,Bcl-2）、半胱天冬酶3（caspase 3,CASP3）和活化半胱天冬酶3（active-caspase 3,Act-CASP3）的表达变化。结果表明,绵羊子宫不同时期DHT的合成和AR的表达存在差异,妊娠期子宫DHT合成及AR表达显著低于卵泡期和黄体期（P<0.05）。10^-10~10^-7 mol·L^-1DHT处理后,P₄合成酶表达显著上调（P<0.05）,在10^-8~10^-7 mol·L^-1 DHT时P₄合成显著增加（P<0.05）,在10^-10~10^-8 mol·L^-1 DHT时孕酮受体蛋白表达显著增加（P<0.05）,但10^-7mol·L^-1 DHT时孕酮受体蛋白表达显著下降（P<0.05）;在10^-8~10^-7 mol·L^-1 DHT时E₂相关合成酶显著减少,并且E₂水平显著下降（P<0.05）,在10^-9和10^-7 mol L^-1 DHT时E₂受体ERα蛋白显著下调（P<0.05）,在10^-10~10^-7 mol·L^-1 DHT时ERβ和GPER显著增加（P<0.05）。经Flu处理后部分解除DHT对P₄和E₂的调控。此外,10^-10~10^-7 mol·L^-1 DHT显著促进子宫内膜上皮细胞凋亡（P<0.05）。本研究证实DHT至少部分通过AR调节P₄和E₂合成及受体表达,影响细胞凋亡,参与调节子宫功能,这为进一步阐明雄激素参与调节子宫功能提供了新的基础和相关数据。  相似文献   

SCINTIGRAPHIC MEASUREMENT OF UTERINE CLEARANCE IN MARES     

L. Neuwirth  DVM  MS  M.M. Leblanc    DVM  D. Mauragis  CVMT  E. Klapstein  MS  T. Tran  PhD 《Veterinary radiology & ultrasound》1995,36(1):64-68

Uterine clearance of technetium 99m-albumin colloid (^99mTc-μAA) was qualitatively and quantitatively measured in 5 reproductively normal mares and 5 mares susceptible to endometritis (infertile). The percentage of 370 MBq ^99mTc-μAA cleared from the uterine lumen within 2 hr of intrauterine infusion was measured in 10 mares on day 3 of estrus and 48 hr after ovulation. The procedure was repeated 3 times on day 3 of estrus in 6 mares to determine repeatability. Six mares were infused with 1110 MBq ^99mTc-μAA on day 3 of estrus to evaluate the effect of increasing the dose to reduce the imaging time. There was no statistically significant difference in the mean percentage of radiocolloid cleared from the uterus during day 3 of estrus or 48 hr after ovulation or in the percent cleared when the studies were repeated in individual mares. There was no statistically significant difference in uterine clearance between the 370 and 1110 MBq dose studies in each mare from 15 to 120 min. Reproductively normal mares cleared approximately 50% of the radiocolloid from the uterus by 120 min while susceptible mares cleared less than 15%.  相似文献   

多胎动物的子宫容量及其遗传选择     

昌文林  刘正年  周智  薛立群 《中国畜牧兽医》2008,35(11):94-97

子宫容量是对子宫维持多个孕体正常孕育能力的描述。20世纪60年代以来,对多胎动物的子宫容量及其遗传选择进行了较系统的研究。对此作者着重讨论和综述了多胎动物的子宫容量、子宫容量的度量及子宫容量的遗传选择。  相似文献   

亚临床酮病对泌乳早期奶牛繁殖性能的影响   总被引：1，自引：0，他引：1  

张锋  白云龙  宋玉锡  孙书函  夏成  徐闯  张洪友 《现代畜牧兽医》2019,(6):49-52

为探究围产期亚临床酮病与泌乳奶牛繁殖性能及卵泡发育之间的关系,本试验选择了黑龙江省某大型集约化牛场开展试验,根据产后血酮水平确定亚临床酮病组和健康组奶牛共80头,该牛场亚临床酮病发生率为71%;两组奶牛跟踪到产后50 d进行直肠检查和B超检查了解卵泡发育状况,并记录繁殖性能数据。结果显示:与健康组奶牛相比,亚临床酮病组奶牛产后首次发情天数延长10 d,产后60 d内发情次数少0.58次;产后50 d子宫复旧不全高15%,与亚临床酮病呈显著正相关(R^2=8.74);产后60 d卵泡直径小3 mm(P<0.05)。结论:该牛场奶牛亚临床酮病发病率较高,是引起奶牛产后繁殖障碍的风险因素,也是造成卵泡发育受阻的不良因素,会导致奶牛繁殖力下降。  相似文献   

中国荷斯坦牛产后0～35d繁殖疾病遗传参数估计     

董祎鑫  李想  亓建刚  罗汉鹏  窦金焕  刘林  李锡智  王雅春 《畜牧兽医学报》2019,(2):280-286

旨在估计中国荷斯坦牛产后0～35d的繁殖疾病遗传参数。本研究收集了1993-2017年间北京地区27个场中国荷斯坦牛产后0～35d25 026条繁殖疾病记录,统计分析发病规律,采用阈模型和线性模型,以场年、胎次和产犊季节为固定效应,个体加性遗传效应和永久环境效应为随机效应得到遗传参数来探究中国荷斯坦牛产后0～35d繁殖疾病遗传规律,同时尝试单独对子宫疾病进行遗传参数估计。结果显示,奶牛产后0～35d繁殖疾病记录占整个泌乳期繁殖疾病记录的54%;使用线性模型和阈模型所得奶牛产后0～35d遗传力分别为0.015 2(0.001 9)和0.094 4(0.01),使用SAS计算两个模型繁殖疾病EBV排名秩相关达95%(P<0.01);在繁殖疾病中,子宫疾病占48%,且主要集中于产后0～35d,利用线性模型估计得到产后0～35d子宫疾病遗传力为0.010 7(0.001 6)。根据线性模型得到的EBV计算繁殖疾病遗传趋势,发现自2000年以来,公母牛繁殖疾病、子宫疾病估计育种值均呈现下降趋势。本研究结果可为中国荷斯坦牛繁殖疾病的遗传育种工作提供参考。  相似文献   

利福昔明子宫注入剂稳定性及其对家兔阴道黏膜刺激性研究     

徐飞  刘静  黄慧丽  陈孝杰  李秀波 《中国畜牧兽医》2019,46(9):2795-2802

为研究利福昔明子宫注入剂的稳定性,从而初步确定其保质期,本试验取3批利福昔明子宫注入剂进行稳定性试验考察,包括影响因素、高温加速及长期稳定性,利用高效液相色谱法测定试验过程中利福昔明含量及有关物质的含量,从而初步确定利福昔明子宫注入剂的保质期。根据相关技术指导原则对家兔进行阴道刺激性试验。稳定性试验结果表明,根据面积归一化法计算有关物质的含量均不超过3%,注入剂的性状、沉降、粒度未发生明显变化,主成分利福昔明的含量变化在拟定标示量90.0%~110.0%范围内,从而确定本品的有效期为2年。家兔阴道刺激性试验结果显示,利福昔明子宫注入剂给药部位（阴道）和生理盐水对照组给药部位都没有出现红斑和水肿,刺激强度的评分为0;试验组和对照组的阴道黏膜没有出现明显的充血、水肿及异常分泌物流出的现象,阴道黏膜刺激指数为0.25。因此,利福昔明子宫注入剂对家兔的阴道无刺激性。本品的临床安全性良好,可进一步推广使用。  相似文献   

Sprinkling coarse marble powder during the afternoon, toward the end of the laying period, may increase eggshell quality     

Guray ERENER  Nuh OCAK  Ali Vaiz GARIPOGLU  Ergin OZTURK 《Animal Science Journal》2008,79(3):354-361

The aim of this study was to investigate the effects of time, particle size (PS) and level of calcium supplementation above the requirements toward the end of the laying period on egg production and eggshell quality. A total of 675 Isa-Brown layer hens were distributed in a completely randomized factorial arrangement of 3×3×3, using three sprinkling times (08.00, 12.00, 16.00 hours), PC (mash, granular and large size) and amounts (0 g control, 0.75 g/day/h, 1.50 g/day/h) of marble sprinkling as a calcium source. All hens were fed a commercial layer diet from 55 to 67 weeks of age. Sprinkling of 1.50 g marble reduced ( P  < 0.05) egg production compared to 0.75 g marble, and feed efficiency (FE) compared to that of 0 and 0.75 g marble. Sprinkling of 0.75 and 1.50 g marble increased ( P  < 0.01) eggshell weight, shell strength and shell thickness compared to that of 0 g marble. The interaction of sprinkling time-by-PS-by–quantity of marble was significant for egg production ( P  < 0.05), FE and shell strength ( P  < 0.01). Results showed that sprinkling of coarse marble powder to complete diet increased shell strength without reduction in egg production and FE.  相似文献   

Peripheral Concentration of Hydroxyprolineand 3-methyl-histidine in Postpartum Cow     

TIAN Wen ru  HE Jian bin 《东北农业大学学报(英文版)》2000,7(1)

1　Introduction　　 It has been show in rat(Harkness and Harkness,1 95 4) ,human(Montford and PereaTamayo,1 96 1 ) and cattle (Kaidi,1 989) that the increase in the weight of the uterus duringpregnancy is associated with an increase in the collagen content of the uterus.Themechanisms involved in timulating collagen synthesis are unknown.Atthe same time there ishypertrophy and hyperplasia of the smooth muscle fibers of the myometrium.In the post-partum period,the involutionary changes of the…  相似文献