野生茄"托鲁巴姆"保护地繁种技术   总被引：1，自引：1，他引：0  

王贵余 《北方园艺》2004,(1):21-21

利用野生茄"托鲁巴姆"作砧木进行茄子嫁接栽培,可以增强茄子的抗低温能力,且高抗茄子黄萎病、枯萎病、青枯病、根线虫病等多种土传病害.特别是嫁接苗中后期植株生长旺盛,生长量大,茄子产量高、品质好.因此,"托鲁巴姆"是目前生产上最佳的茄子砧木品种,深受广大农民的喜爱.  相似文献   

1%苦参碱·印楝素EC（托盾）0.36%苦参碱AS防治茶树害虫试验报告     

刘源  刘贵芳 《茶叶通讯》2011,(4):19-21

1%苦参碱·印楝素EC（托盾）,防治茶树害虫茶小绿叶蝉、茶毛虫、茶尺蠖均有较好防治效果,可以大力在郴州市茶叶绿色食品、有机茶产区推广应用,喷施浓度以托盾800倍液为好。  相似文献   

托收融资风险问题研究     

陶芸 《绿色科技》2009,(7)

国际经济贸易活动中,进出口双方常常通过托收融资来防范托收结算方式下的风险,但是不论是出口托收押汇还是进口托收押汇,它们本身也蕴藏着一定的风险,只有认真地研究这些风险并加以防范,才能更好的让银行的托收融资业务为企业服务.  相似文献   

马来西亚原木上截获的托氏白条天牛     

张红英  王学  王凯  季宏铁  沈其林  吴初效 《中国农学通报》2009,(3)

本文记述了嘉兴乍浦口岸从进口马来西亚原木上检疫截获的具有潜在入侵风险的托氏白条天牛(Batocera thomsoni Javer,1858),对其形态特征作了详细描述,并提供了与其相似的7种白条天牛的检索表.  相似文献   

Correction of the Mutant SLA-1-632-TPK Gene from ToPigs Pig and Construction of SLA-1-TPK/pMD19-T Recombinant Plasmid     

ZHAI Xiao-xin  GAO Hua  HAN Yong  GAO Feng-shan 《中国畜牧兽医》2017,44(6):1645-1651

The swine leukocyte antigen (SLA) genes in pigs were the important immune gene group in antigen presentation, and studing on SLA could provide the references for the prevention of some infectious diseases. Earlier studies found that SLA-1-632-TPK gene in ToPigs pig had a deleted base in its coding sequence (a single base "C" was lost in 632 bp from the 5' end of the SLA-1-632-TPK gene), which lead to frameshift mutation. In order to correct the SLA-1-632-TPK gene, two pairs of gene-correction's primers were designed to correct the gene by the splicing overlap extension PCR (SOE-PCR) in template of recombinant plasmid of SLA-1-632-TPK/pMD18-T. Firstly,the 5'and 3'ends of SLA-1-632-TPK gene were amplified, respectively, then both of them were spliced and amplified to form an intact SLA-1-632-TPK gene. After detected by agarose electrophoresis, the interest of the product was further cloned into pMD19-T Simple vector. The positive clones were screened by colony PCR and then sequenced. The result showed that the 5'and 3' ends of the SLA-1-632-TPK gene were all amplified successfully by SOE-PCR, with the products of about 650 and 590 bp, which were consistent with the theoretical value of 648 and 585 bp, respectively. After spliced, the intact sequence of SLA-1-632-TPK gene was obtained with the product of about 1 200 bp, which was close to the theoretical value of 1 223 bp. The colony PCR result showed that the corrected gene was successfully inserted into pMD19-T Simple vector . After the sequence was analyzed by GENETYX version 9.0, it was shown that the nucleotide "C" in 632 bp numbered from the 5'end of the gene was added and the SLA-1-632-TPK gene was coded correctly. In this study, the SLA-1-632-TPK was corrected successfully, and the recombinant plasmid SLA-1-TPK/pMD19-T was constructed, which would lay a foundation to further study the protein expression and associated function of SLA-1-TPK.  相似文献   

托佩克猪SLA-1-632-TPK基因的矫正及重组质粒SLA-1-TPK/pMD19-T的构建     

翟晓鑫  高花  韩勇  高凤山 《中国畜牧兽医》2017,44(6):1645-1651

猪白细胞抗原（swine leukocyte antigen,SLA）在猪免疫系统中起递呈抗原作用,对其展开研究可为猪相关传染病的预防提供依据。研究发现,托佩克猪SLA-1-632-TPK基因编码序列发生碱基缺失（距离SLA-1-632-TPK基因5'端632 bp处丢失1个碱基"C"）,导致移码突变。为矫正SLA-1-632-TPK基因,设计2对矫正引物,以原重组质粒SLA-1-632-TPK/pMD18-T为模板,利用剪切重叠延伸PCR（splicing overlap extention PCR,SOE-PCR）技术分别扩增SLA-1-632-TPK 5'和3'端,之后进行拼接,最后扩增全序列从而矫正目的基因,并进一步连接pMD19-T Simple载体,通过单菌落PCR筛选阳性克隆并测序,并通过GENETYX version 9.0软件对所测序列进行分析。结果显示,SOE-PCR成功扩增得到5'和3'端片段,大小约为650和590 bp,与理论设计值大小（648和585 bp）接近,经过拼接以后,得到全长约1 200 bp,与理论设计值1 223 bp接近。菌落PCR结果显示,矫正基因成功克隆入pMD19-T Simple载体。序列分析结果显示,托佩克猪SLA-1-632-TPK基因距离5'端632 bp处丢失的碱基"C"得到矫正并正确编码。本研究成功矫正了SLA-1-632-TPK基因,并构建其重组质粒SLA-1-TPK/pMD19-T,为下一步研究SLA-1-TPK蛋白表达和相关功能奠定基础。  相似文献   

温室茄子长季节栽培生产高效技术     

周振林 《吉林蔬菜》2010,(6):21-22

<正>1选择优良品种接穗:选择耐低温、早熟、耐弱光、抗病的布利塔。砧木:选择对黄萎病、枯萎病免疫的野生托鲁巴姆茄子。2培育壮苗2.1确定适宜播期及播种量:砧木一般在6月上中  相似文献   

术前鼻内滴注右美托咪定对预防异丙酚注射痛的临床观察     

郭迎霞  陈金仙  张良清 《湛江医学院学报》2013,31(2):185-187

目的观察术前鼻内滴注右美托咪定(DEX)对预防异丙酚注射痛的效果。方法 80例择期行妇科腹腔镜手术的患者随机分为DEX组和对照组,每组40例。麻醉诱导前30min,DEX组鼻内滴注DEX,对照组给相同容量的生理盐水。比较两组异丙酚注射痛的发生率和严重程度,观察给药后血流动力学改变。结果 DEX组对异丙酚注射痛的发生率和严重程度与对照组比较均明显降低(P<0.01);DEX组异丙酚麻醉的诱导用量与对照组进行比较明显减少[(67.4±12.7)mgvs(86.1±13.3)mg,P<0.01]。结论术前30min鼻内预注DEX能有效的减轻异丙酚注射痛和减少异丙酚麻醉诱导的需要量。  相似文献   

2013年前4个月小麦市场回顾与后期展望     

孙超 《农业展望》2013,9(5):4-8,12

2013年前4个月国内小麦价格以稳为主。2013年春节过后,在市场粮源有限和制粉企业需求疲弱的双重影响下,小麦市场连续3个月保持弱势平衡状态,较高的政策性小麦投放力度保证了市场供给和价格稳定。在新季小麦上市前,预计市场供需格局不会发生根本改变。后期小麦价格一方面取决于新季小麦产量,另一方面也要受到政策取向的显著影响。在综合分析小麦市场基本面和政策面各影响因素的基础上,对后期小麦市场进行展望,认为2013年中国小麦价格将以温和上涨走势为主。  相似文献   

《中国蔬菜》2013·10 学术论文导读     

《中国蔬菜》2013,1(9):24-25

托鲁巴姆(Solanum torvum Sw.)在茄子嫁接栽培上的应用研究李楠洋等(中国农业科学院蔬菜花卉研究所,北京100081)—《中国蔬菜》2013(10)茄子近缘野生种托鲁巴姆(Solanum torvum Sw.)具有良好的抗病、抗逆性,作为砧木在茄果类蔬菜嫁接生产上得到广泛应用,显示出良好的抗病增产效  相似文献