Adult rat and human bone marrow mesenchymal stem cells differentiate into neurons with Musk's polypeptide     

XIAO Qing-zhong  LI Hao-wei  WEN Guan-mei  HUANG Shao-hua ZHANG Xiu-ming  LI Yan  LI Shu-nong 《园艺学报》2002,18(10):1179-1182

AIM: To investigate the differentiation from adult rat and human bone marrow mesenchymal stem cells (BMMSCs) into neuron with musk polypeptide (Mu-P).METHODS: Adult rat and human BMMSCs were induced with Mu-P.Neuron-specific enolase (NSE),neurofilament (NF),Nestin,glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry.RESULTS: Simple methods with Mu-P induced adult rat and human BMMSCs exhibiting a neuronal phenotype,expressing Nestin at 3 hours to 5 hours,and expressing NE and NF at 5 hours to 7 days.But the neuron-like cells didn't express the glial astrocyte marker GFAP.CONCLUSION: Adult rat and human BMMSCs can be induced to differentiate into neurons with Mu-P.  相似文献   

Inhibitory effect of berberine on the activation and proliferation of T lymphocytes     

HE Xian-hui  ZENG Yao-ying  XU Li-hui  QIU Hai-xia  CAI Xiao-chang 《园艺学报》2002,18(10):1183-1186

AIM: To investigate the effect of berberine (Ber) on the activation and proliferation of T lymphocytes and its mechanism of action. METHODS: Whole peripheral blood from normal subjects was stimulated with phytohemagglutinin (PHA) or phorbol ester (PDB) plus ionomycin (Ion) and the expression levels of CD69 and CD25 were evaluated with flow cytometry after the staining with appropriate fluorescent monoclonal antibody. The distribution of cell cycles was analyzed by propidium iodide staining and dead cells by 7-aminoactinomycin live staining. RESULTS: 100 μmol/L and 50 μmol/L of Ber had significant inhibition of the expression of CD69 on T cells stimulated with PDB plus Ion or PHA, while effect of 25 μmol/L Ber was not significant. And as time of action extended, the extent of inhibition decreased. For the expression of CD25, Ber at the concentrations as above all exerted significant inhibitory effect in a dose-dependent manner. Moreover, Ber could block lymphocytes cell cycle progression from G₀/G₁ phase to S and G₂/M phase without phase specificity. Besides, live staining analysis revealed that Ber did not have significant cytotoxicity on lymphocytes. CONCLUSIONS: Ber significantly inhibits the expression of early and mid activation antigens of T cells and also blocks the progression of lymphocytes cell cycles. These results suggest that Ber exerts immunosuppression effect through inhibiting the activation and proliferation of T cells.  相似文献   

Protein expression of cyclin D₂ and p16 in proliferation and differentiation of cultured cardiac myocytes     

ZHANG Yu-xia  YU Lun-yin  LIU Ming-qiu  ZHANG Zheng-bin TANG Zhi-jiao  XIA Dong  WANG Ming 《园艺学报》2002,18(1):32-35

AIM:To investigate the protein expression of cyclin D₂ and p16 in proliferation and differentiation of cultured cardiac myocytes.METHODS:One-day-old Sparague-Dawley rats were used. Cardiac myocytes(CM) were collected by a trypsin-dispersal method and cultured. Cell growth line and fluorescence activated cell sorting (FACS) were used to investigate the proliferation of CM. Ultra-thin sections were made to observe the ultrastructure of CM under transmission electron microscope. The expression of cyclin D₂ and p16 in CM were measured using immunocytochemistry and image analysis.RESULTS:①Results of cell growth line and FACS analysis showed that cultured CM could proliferate in the first 3 cultured days, but the ability decreased quickly, concomitant with differentiation. CM was obseved quiescent in cell cycle three days later. The ultrastructure of CM showed the large amount of myofilaments and mitochondrion. ②The protein expression of cyclin D₂ in 3,4,5 day CM group was 0.89 times(P＜0.05),0.80 times (P＜0.05) and 0.56 times (P＜0.01) of that in 1 day group, respectively. The expression of p16 in CM was increased during the culture process, 2,3,4,5 day group were 1.63 times, 1.72 times, 1.99 times and 2.84 times (P＜0.01) of that in 1 day group, respectively.CONCLUSION:Cultured neonatal rat cardiac myocytes could proliferate during the first 3 days after incubation, but the ability of proliferation decreased, from the fourth day, concomitant with differentiation. Cyclin D₂ and p16 play the key roles in CM postnatal development. Downregulation of cyclin D₂ and upregulation of p16 may induce CM differentiation.  相似文献   

Effects of hydrogen peroxide on cell proliferation and expression of gelatinase A and its inhibitor in vascular smooth muscle cells     

GUO Xiao-gang  CHEN Jun-zhu  ZHU Jian-hua  ZHENG Liang-rong  ZHANG Fu-rong  TAO Qian-min  ZHANG Yun 《园艺学报》2002,18(12):1524-1528

  相似文献   

我国农药残留快速检测技术的研究与应用现状   总被引：21，自引：1，他引：21  

涂忆江 《农药科学与管理》2003,24(4):14-16

本文简述了目前农药残留快速检测技术的研究概况，分析了农药残留快速检测技术在我国的实际应用情况。  相似文献   

温湿度调控对番茄灰霉病菌产生的细胞壁降解酶的影响   总被引：6，自引：0，他引：6  

李宝聚  陈立芹  孟伟军  王福建 《植物病理学报》2003,33(3):209-212

 番茄灰霉病菌在致病过程中能够产生4种细胞壁降解酶,以PMG酶活性最高,其次是β-葡萄糖苷酶和PG酶,Cx最少。灰霉病菌在不同温度下侵染番茄叶片时产生的致病酶活性不同,4种酶在20℃时表现了最高的活性,15℃次之,当温度达到25℃时,各种酶的活性都急剧下降,随着温度的再升高,酶活更低。随着湿度的增高,病菌产生的细胞壁降解酶的活性也增加,当相对湿度达到90%以上时,4种酶的活性也达到最高。温湿度对番茄灰霉病菌产生细胞壁降解酶的影响趋势,与其对发病的影响趋势是一致的。  相似文献   

低温胁迫下弓葵幼苗膜脂过氧化及保护酶活性的变化   总被引：32，自引：0，他引：32  

杨盛昌  谢潮添  张平  陈德海  丁印龙  廖启炓 《园艺学报》2003,30(1):104-106

 低温胁迫下弓葵( Butia capitata Becc) 幼苗叶片的MDA 含量逐渐增加, 膜脂过氧化作用增强。- 8 ℃条件下的膜脂过氧化作用明显强于2 ℃。细胞膜透性在2 ℃条件下变化不大, - 8 ℃时则随低温胁迫时间延长而急剧上升, 细胞膜受到伤害。- 8 ℃胁迫下细胞保护酶SOD、POD 和CAT 活性短期(6 h) 内升高,然后下降, 24 h 以后3 种保护酶受到低温胁迫的严重抑制。在2 ℃胁迫下, SOD 活性在6 h 内变化不大, 随后下降; CAT活性变化趋势与- 8 ℃时相似, 但变化幅度较小; POD 虽也呈现先升后降的趋势, 但降幅明显小于升幅, 至48 h 时POD 活性仍维持较高水平。2 ℃低温胁迫不是抑制而是促进POD 活性的提高。  相似文献   

肥城桃两品系果实细胞壁成分和水解酶活性的比较   总被引：17，自引：0，他引：17  

薛炳烨  束怀瑞 《园艺学报》2004,31(4):499-501

 随着果实发育成熟，肥城桃两品系果实中细胞壁含量不断下降。可溶性果胶从8月1～16日明显增加，这时果肉硬度下降最快。两品系果实的细胞壁中离子结合果胶和纤维素含量下降比例不同，这可能是造成两品系成熟软化特性不同的主要因素之一。果实发育后期‘白里肥城桃’和‘红里肥城桃’纤维素酶活性变化的不同，意味着纤维素酶在肥城桃果实软化中起重要作用。肥城桃果实中没有检测到内切PG活性，成熟后期外切PG活性上升较快。  相似文献   

钙及其拮抗剂对苹果果肉质膜透性的调节作用   总被引：5，自引：0，他引：5  

关军锋  束怀瑞  张良诚 《果树学报》2004,21(5):402-405

采用培养果肉圆片的方法,研究了Ca2+及其拮抗剂对苹果果肉质膜透性的调节作用。结果表明,CaCl2(1、10mmol/L)降低果肉膜透性和溶质外渗速率(Js);细胞膜Ca2+通道阻塞剂Verapamil(100μmol/L)的影响不显著;细胞外Ca2+螯合剂EGTA(5mmol/L)、CaM的拮抗剂CPZ、TFP(100μmol/L)明显提高果肉膜透性和细胞溶质外渗速率。培养24h时,CaCl2能明显维持较高的SOD活性和ACC向乙烯的转化能力,EGTA、Verapamail、CPZ和TFP的作用相反。这些说明Ca2+对果肉细胞膜具有保护作用,而减少细胞外Ca2+和抑制细胞内Ca2+-CaM功能对果肉细胞膜具有伤害作用。  相似文献   

Model establishment and biological behaviour observation of mouse blastocyst co-cultured with hepatocarcinoma cell lines with differently invasive and metastatic potential in vitro     

LI Da-qiang  WANG Zhi-biao  BAI Jin  ZHAO Jie  HU Kai  WANG Yuan  DU Yong-hong 《园艺学报》2004,20(1):32-37

AIM: To explore interaction and biological behaviour changes of two kinds of cells-blastocysts and hepatocarcinoma cells in the same microenvironment. METHODS:The models of mouse blastocysts co-cultured with human hepatocarcinoma cell lines were established, then biological behaviours and mutual effects of the two kinds of cells in co-culture system were observed. RESULTS: Compared with control group, hepatocarcinoma cells with differently invasive and metastatic potential significantly enhanced the rates of blastocyst hatchment , attachment and outgrowth(P＜0.05). There was no significant difference in those among hepatocarcinoma cells co-cultured groups (P＞0.05). The blastocyst hatched and attached to hepatocarcinoma cells with differently invasive and metastatic potential. Then, differential trophoblasts invaded hepatocarcinoma cells. The clear-cut interfaces were gradually formed between both sides. Hepatocarcinoma cells on interface showed changes of growth direction and cell shapes and did not invade blastocysts. CONCLUSIONS: Hepatocarcinoma cells promoted blastocyst development. Blastocysts implanted and invaded hepatocarcinoma cells with differently invasive and metastatic potential in vitro, which indicate that blastocyst implantation in vitro does not relate with the kinds and differential level of interactional cells and the low selectivity maybe relate with high adaptability of early life.  相似文献