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1.
以乳白期‘春星’草莓(Fragaria ananassa Duch．‘Chunxing’)果实为试材，研究了经钙调素(CaM)拮抗剂三氟拉嗪(TFP)、氯丙嗪(CPZ)各100 µmol·L-1 和钙通道阻塞剂异博定(Verapamil)100 µmol·L-1 预处理后再用乙烯(50 µL·L-1 )处理的草莓果实中微粒体“Ca2+-ATP酶活性、O2 产生速率和MDA含量的变化。结果表明，外源乙烯对微粒体膜O2 产生速率无显著影响，处理早期提高微粒体膜Ca2+- ATPase总活性，对MDA含量影响不大；后期加速微粒体膜Ca2+- ATPase总活性下降，但仍保持较高的MDA含量和线粒体膜Ca2+-ATPase活性。CPZ、TFP和Verapamil预处理降低了上述乙烯处理下Ca2+- ATPase活性和MDA含量，但对微粒体膜O 产生速率亦无显著影响，这说明细胞内Ca2+-和CaM 可能参与了乙烯诱导的膜Ca2+-ATP酶活性与膜脂过氧化水平的调节。 相似文献
2.
用两种从不同位点阻断Ca2+信号途径的抑制剂,即Ca2+螯合剂EGTA(乙二醇双四乙酸)(5 mmol/L)和CaM拮抗剂W7(N-氨乙基-5-氯-1-磺胺酰萘)(0.3 mmol/L),分别处理盐胁迫(0.5 mmol/L)下的野生苦菜幼苗,测定其抗氧化系统中酶的活性,H2O2、Pro和可溶性蛋白的含量。结果表明,EGTA(5 mmol/L)、W7(0.3 mmol/L)可降低超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性,提高H2O2的含量,降低Pro和可溶性蛋白的含量,说明Ca2+-CaM系统与野生苦菜耐盐性密切相关。 相似文献
3.
草莓和番茄果实乙烯自我催化与Ca2+-CaM 的关系 总被引：3，自引：1，他引：3
外源乙烯处理乳白期草莓和绿熟期番茄果实12 h后，诱导草莓乙烯大量合成，使番茄乙烯释放高峰提前2 d出现，同时还促进两种果实的钙调素(Calmodulin，CaM)含量增加。细胞质膜钙离子通道阻塞剂异搏定(Verapamil，Vp)、钙调素拮抗剂氯丙嗪(Chloropromaize，CPZ)和三氟拉嗪(Trifluoperazine，TFP)均抑制了外源乙烯诱导的草莓乙烯合成，表明Ca2+-CaM 信使系统可能参与草莓乙烯自我催化作用；Vp抑制外源乙烯诱导的番茄乙烯合成，而CPZ和TFP的作用不显著，说明番茄果实乙烯自我催化作用与胞外Ca2+ 内流有关，与CaM的关系不明显。 相似文献
4.
以"陕林4号"和"07-69×青1"为试材,分别用浓度为0、5、10、15、20mmol/L的CaCl2溶液喷施杨树幼苗,4℃低温胁迫2d后测定杨树幼苗的膜伤害率、丙二醛(MDA)含量、抗氧化酶(SOD和POD)活性以及可溶性蛋白质含量等生理指标,以探究低温胁迫下不同浓度外源Ca2+对杨树幼苗抗寒相关生理指标的影响。结果表明:在喷施10mmol/L CaCl2的作用下,杨树幼苗的各项抗寒相关生理指标最优,可显著降低杨树幼苗的膜伤害率,减少丙二醛(MDA)含量,提高超氧化物歧化酶(SOD)活性和过氧化物酶(POD)活性,增加可溶性蛋白质含量。外源Ca2+可以有效提高杨树幼苗的抗寒能力,缓解低温胁迫对杨树幼苗的伤害。 相似文献
5.
钙处理对乙烯诱导的番茄离体花柄脱落的抑制作用 总被引：1，自引：0，他引：1
以番茄‘辽园多丽’的小花为材料, 研究外源乙烯( 20μL ·L-1 ) 处理下, 花柄脱落过程中, 离区微粒体膜Ca2+ -ATPase、羧甲基纤维素钠酶(CMC-Na) 、内切多聚半乳糖醛酸酶( Endo-PG) 和果胶脂酶( PE) 等活性的变化。结果表明: 外源乙烯加速了花柄的脱落, 刺激了其离区的Ca2+ -ATPase活性先升高后下降, 随后离区CMC-Na、Endo-PG、PE活性升高; 钙离子专一性螯合剂EGTA和钙调素拮抗剂TFP均在一定程度上抑制了乙烯诱导的上述反应, 表明细胞内Ca2+与CaM参与了乙烯反应。 相似文献
6.
研究了不同浓度配比的8-羟基喹啉、柠檬酸、蔗糖、邻苯二酚和CaCl2组成的保鲜剂对切花月季花枝鲜重、花径、瓶插寿命、叶绿素含量、细胞膜透性、相对含水量等的影响。结果表明:100 mg/L 8-HQC+0.5%蔗糖+0.2%CaCl2的保鲜剂能够促进月季切花吸水,延迟叶片中叶绿素的分解及细胞质外渗,显著延长月季切花的寿命。 相似文献
7.
钙素对SA诱导番茄幼苗抗灰霉病的调控作用 总被引：1，自引：0，他引：1
为探索钙对水杨酸（SA）诱导番茄（Solanum lycopersicum）幼苗抗灰霉病的作用,采用‘L402’番茄品种,通过分别喷施8 mmol • L-1 CaCl2和5 mmol • L-1 EGTA后再喷施2 mmol • L-1 SA,3 d后接种灰霉病菌孢子的方法,研究了不同处理对番茄幼苗灰霉病病情指数、活性氧积累、主要防御酶活性及其基因表达的影响。结果表明：接种灰霉病菌孢子5 d后,SA处理的番茄幼苗病情指数比对照降低37.27%,Ca + SA处理较SA处理进一步降低18%;接种1 d和2 d后,叶片中产生速率和H2O2含量分别出现应激高峰,且处理间存在差异,与对照相比Ca + SA处理分别提高33.05%和29.31%,EGTA + SA处理分别降低32.62%和46.34%;叶片中抗病相关酶活性和基因表达在接种病菌后也出现应激高峰,其中Ca + SA处理显著提高了PAL、几丁质酶、β–1,3–葡聚糖酶活性,EGTA处理及EGTA + SA处理显著降低了PAL、几丁质酶、β–1,3–葡聚糖酶活性。上述结果表明,Ca2+在SA诱导番茄抗灰霉病中具有正调控作用,而且这种作用与PAL、几丁质酶、β–1,3–葡聚糖酶活性及其基因表达密切相关。 相似文献
8.
梨果实发育中Ca2+在果肉细胞的定位及变化研究* 总被引：17，自引：3，他引：14
用焦锑酸钾沉淀的细胞化学方法, 研究了 幸水 梨果实发育中果肉细胞的焦锑酸Ca2+ 定位变化及其与细胞超微结构的关系。结果表明: ( 1) 在未受精之前, 果肉细胞内未检测到Ca2+ 沉淀颗粒, 细胞核内的染色质少且染色淡, 细胞质的细胞器数量也少; ( 2) 受精后果肉细胞呈现大量的Ca2+沉淀颗粒, 主要分布在细胞核、细胞质、质体以及叶绿体外膜上, 含Ca2+沉淀颗粒的质体非常膨大, 导管和初期发育的石细胞内也密集分布Ca2+ 沉淀颗粒; ( 3) 受精1 周后果肉细胞的Ca2+移向细胞之间的连接处; ( 4) 生理落果的细胞和导管中Ca2+没有或极少, 但有的细胞内沿液泡膜有Ca2+分布; ( 5) 受精3~ 4 周后, 果肉细胞中很难检测到Ca2+沉淀颗粒, 此状态一直持续到果实采收, 但果实腐烂前Ca2+沉淀颗粒沿果肉细胞
壁两侧出现。就Ca2+ 在果实发育中的作用及与细胞超微结构的关系等进行了讨论。 相似文献
9.
10.
果用香蕉薄片外植体植株再生的研究 总被引：17，自引：1，他引：16
利用薄片培养方法, 通过直接和间接器官发生途径分别获得了‘广东631’香蕉的再生植株。该途径受多种因素影响。通过正交试验和单因子试验确定了最佳培养条件: (1)直接器官发生培养基为Ma (MS modified) + BAP 10μmol/L + KT 50μmol/L + IAA 1μmol/L ; (2)愈伤组织诱导培养基为Mb (B5 modified) + Dicamba 10μmol/L + IAA 1μmol/L + 2 ,4-D 0. 7μmol/L + NAA 0. 5μmol/L + BAP 4. 4μmol/L + 活性炭1 g/L ; (3) 愈伤组织继代培养基为Mb + Dicam-ba 5μmol/L + IAA 1μmol/L + 2 ,4-D 0. 4μmol/L + BAP 22μmol/L + KNO3 500 mg/L + 维生素B1 40 mg/L + 活性炭0. 5 g/L ; (4) 分化培养基为1/ 2 Ma + BAP 1μmol/L + IAA 0. 5μmol/L + 活性炭1 g/L ; (5) 成苗培养基为Ma + BAP 1μmol/L + KT 4. 6μmol/L + NAA 1μmol/L。愈伤组织诱导和继代培养需在黑暗中进行。 相似文献
11.
采后浸钙对雪花梨果肉褐变的影响 总被引：31，自引：0，他引：31
雪花梨果实采后常压浸泡６％ＣａＣｌ２１５分钟或减压浸泡４％ＣａＣｌ２２分钟能保持果肉较高水平的酚类物质（酚类物质与果肉钙含量呈显著正相关，ｒ＝０．８６３７），抑制ＦＰＰＯ（游离态多酚氧化酶）活性，维持细胞膜结构的完整性（果肉相对电导率与果肉钙含量呈显著负相关，ｒ＝－０．８７７５）。雪花梨果肉褐变与果肉钙含量及氮钙比有关，含钙量愈低或氮钙比愈高，果肉愈易褐变。重病果与健康果相比，其果肉钙含量低４４．６％，氮钙比高７５．３％。 相似文献
12.
AIM: The effects of selenium dioxide (SeO2) on proliferation, apoptosis, intracellular reactive oxygen species (ROS) and Ca2+ levels in three leukemia cell lines NB4, K562 and HL-60 were investigated. METHODS: Three leukemia cell lines were treated with 3-30 μmol/L SeO2. Flow cytometry was used to detect apoptosis rate, and analyze the changes of ROS and Ca2+ level within cells. RESULTS: SeO2 at 10 and 30 μmol/L inhibited proliferation in three leukemia cell lines. Treatment with 30 μmol/L SeO2 for 48 h induced 54.0%, 46.5%, 49.6% apoptosis in NB4, K562, and HL-60 cells, respectively, and also markedly decreased ROS and Ca2+ levels among three cell lines. The rate of ROS positive cells in NB4 and HL-60 decreased with the increase in SeO2 concentrations. ROS was clearly reduced with 30 μmol/L SeO2 in K562. Ca2+ levels were tardily declined with 10, 30 μmol/L SeO2 in NB4 and HL-60 cells. Ca2+ levels were clearly reduced with 30 μmol/L SeO2 in K562. CONCLUSION: SeO2 induces apoptosis in three leukemia cells. The declines of intracellular ROS and Ca2+ levels are involved in apoptosis induced by SeO2. 相似文献
13.
AIM: To explore the properties of the acetylcholine (ACh)-sensitive potassium channel in type Ⅱ vestibular hair cells (VHCs Ⅱ) in mice saccular macula and the modulation effect of calcium ions.METHODS: Under the whole-cell patch mode,the pharmacology properties of ACh-sensitive potassium channel and the modulation of calcium ions on ACh-sensitive potassium channel were investigated.RESULTS: Following extracellular perfusion of ACh,VHCs Ⅱ displayed a slow and sustained outward current,which was sensitive to tetraethylammonium (TEA,5 mmol/L) and charybdotoxin (CTX,100 nmol/L),but not sensitive to 4-aminopyride (4-AP,15 μmol/L).ACh-sensitive potassium current was inhibited by intracellular application of ethylene glycol-bis (B-aminoethylether)-N,N,N,N-tetraacetic- acid (EGTA,5 mmol/L) and extracellular perfusion of Cd2+ and Ni2+,respectively.Intracellular application of heparin (8 g/L) failed to inhibit ACh-sensitive potassium current.CONCLUSION: Extracellular application of ACh activates the big conductance,calcium-dependent potassium current (BK) in VHCs Ⅱ of mice,which is potently modulated by extracellular Ca2+ ions.However,intracellular IP3-dependent Ca2+ ions release mechanism is not involved in the activation of the ACh-sensitive BK channel. 相似文献
14.
外源钙对根际低氧胁迫下黄瓜植株钾、钙、镁离子含量和ATPase活性的影响 总被引：2，自引：0，他引：2
以黄瓜(Cucumis sativus L.)'新泰密刺'品种为材料,采用营养液栽培,研究了外源Ca2+对根际低氧胁迫下幼苗植株离子含量和ATPase活性的影响。结果表明,常钙低氧处理植株体内K+、Ca2+、Mg2+含量和质膜、液泡膜、内质网膜ATPase活性显著降低;营养液增施4mmol·L-1CaCl2明显缓解了低氧胁迫对植株的伤害,根中K+、Ca2+、Mg2+含量,根系质膜、液泡膜、内质网膜H+-ATPase和Ca2+-ATPase活性,显著高于常钙低氧处理,接近或达到对照水平;根际低氧胁迫下营养液增施Ca2+通道抑制剂La3+(50μmol·L-1H+)显著,降低了幼苗体内K+、Ca2+含量,但对Mg2+含量影响不显著,营养液增施La3+显著降低了质膜H+-ATPase和Ca2+-ATPase的活性,但对液泡膜,内质网膜H+-ATPase和Ca2+-ATPase活性影响不显著。外源钙通过增加黄瓜体内矿质营养离子的吸收和转运,维持质膜、液泡膜和内质网膜ATPase活性,从而缓解低氧胁迫对植株造成的伤害,增强黄瓜植株的低氧耐性。 相似文献
15.
AIM: To investigate the synergistic effect of decitabine (DCA) and valproic acid (VPA) on apoptosis and cell cycle arrest at G0/G1 phase in gastric cancer MGC-803 cells. METHODS: Gastric cancer MGC-803 cells were used in the study and divided into the following groups according to the treatment with different drugs for 72 h: DCA 1.5 μmol/L,DCA 3.0 μmol/L, VPA 1.5 mmol/L, DCA 1.5 μmol/L+VPA 1.5 mmol/L and DCA 3.0 μmol/L+VPA 1.5 mmol/L. The early and late apoptotic rates were detected by annexin V and PI staining. The cell cycle was also determined by flow cytometry. The relative nm23-H1 mRNA expression level was measured by real-time quantitative PCR. RESULTS: The apoptotic rates in VPA 1.5 mmol/L+DCA 1.5 μmol/L group (early: 33.58%±3.88%; late: 31.52%±4.20%) and VPA 1.5 mmol/L+DCA 3.0 μmol/L group (early: 42.61%±4.23%; late: 38.01%±3.86%), the percentages of the cells in G0/G1 phase in VPA 1.5 mmol/L+DCA 1.5 μmol/L group (61.55%±2.38%) and VPA 1.5 mmol/L+DCA 3.0 μmol/L group (66.75%±2.48%), and the relative nm23-H1 mRNA expression levels in VPA 1.5 mmol/L +DCA 1.5 μmol/L group (1.84±0.46) and VPA 1.5 mmol/L+DCA 3.0 μmol/L group (3.02±0.36) were all significantly higher than those in the corresponding concentrations of single drug treatment groups (P<0.01). CONCLUSION: Synergistic effect of VPA and DCA on apoptosis and cell cycle arrest in gastric cancer MGC-803 cells is possibly via inactivation of nm23-H1 gene expression. 相似文献
16.
黄冠梨采后1-MCP和CaCl2处理对品质和果皮褐斑发生的影响 总被引：2，自引：1，他引：1
对采后黄冠梨进行1 000 nL · L-1 1-MCP 熏蒸（1-MCP）、4% CaCl2（Ca）浸泡和二者结合（1-MCP +Ca）处理,结果表明,与对照（未进行1-MCP、Ca 处理）相比,Ca、1-MCP 和1-MCP + Ca 各处理保持了冷藏期间黄冠梨果实较高的硬度、可滴定酸和叶绿素含量,降低了果皮褐斑指数,但对可溶性固形物和维生素C 含量影响较小,并不同程度降低了果实的乙烯释放速率峰值,还显著降低了果皮可溶性酚含量和多酚氧化酶（PPO）活性,抑制果皮细胞膜透性和H2O2 含量的升高,但对呼吸速率的影响较小。Ca、1-MCP + Ca 处理显著提高了果皮和果肉中钙含量。综合看来,以1-MCP + Ca 处理对维持冷藏期间果实品质的效果最好。1-MCP 和Ca（主要是Ca）抑制果皮酚类物质生成和维持细胞膜结构的完整性是其明显减少黄冠梨果皮褐斑的重要原因。 相似文献
17.
WU Li-ping SHEN Fang LIN Lin LU Yuan XIA QiangWU Li-ping SHEN Fang LIN Lin LU Yuan XIA Qiang 《园艺学报》2007,23(1):155-159
AIM: The purpose of this study was to investigate the effect of cyclosporin A on the isolated mitochondria from normal and ischemic rat brains and to observe the possible effect of mitochondrial ATP sensitive potassium channel on mitochondrial permeability transition. METHODS: The swelling of mitochondria isolated from normal and ischemic rat brain was evaluated by pectrophotometry. RESULTS: Cyclosporin A at concentrations of 0.5 μmol/L or 1 μmol/L and diazoxide at concentration of 30 μmol/L significantly decreased the swelling of the normal brain mitochondria induced by 200 μmol/L calcium, which was abolished by atractyloside at 100 μmol/L. However, cyclosporin A at concentration of 5 μmol/L did not affect the mitochondrial swelling. On the mitochondria isolated from ischemic brain, cyclosporin A at 0.5 μmol/L but not 1 μmol/L significantly decreased the mitochondrial swelling, which was cancelled by atractyloside at concentration of 100 μmol/L. Diazoxide at concentration of 30 mol/L had the similar effect with cyclosporin A at 0.5 μmol/L, which was locked by atractyloside at 100 μmol/L or 5-hydroxydecanoate at 100 μmol/L and 200 μmol/L. CONCLUSIONS: Compared with the mitochondria isolated from normal brain, mitochondria from ischemic brain are more sensitive to the inhibition of mitochondria permeability transition pore opening. Activation of mitochondrial ATP potassium channel may be one of the mechanisms by which the opening of mitochondrial permeability transition pore is inhibited. 相似文献