Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy to diagnose osteoarthritis in equine serum     

M. Paraskevaidi  P. D. Hook  C. L. M. Morais  J. R. Anderson  R. White  P. L. Martin-Hirsch  M. J. Peffers  F. L. Martin 《Equine veterinary journal》2020,52(1):46-51

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Improved fireblight diagnostics using quantitative real-time PCR detection of Erwinia amylovora chromosomal DNA     

M. Pirc  M. Ravnikar  J. Tomlinson  T. Dreo 《Plant pathology》2009,58(5):872-881

Specific and sensitive TaqMan real-time PCR assays were developed targeting chromosomal DNA of Erwinia amylovora ( ams C gene and ITS region). These assays increased the reliability of detection of E. amylovora strains, regardless of their plasmid profile, and have the ability to differentiate between Erwinia spp. strains from Hokkaido, Erwinia pyrifoliae and Erwinia spp. isolated from necrotic pear blossoms in Spain. The assays were used for testing the efficiency of three different extraction methods to remove plant-based PCR inhibitors. Combined with an automated DNA-extraction method based on magnetic beads (QuickPick™), the real-time PCR assays reliably detected at least 10³ cells mL^−l ( c. four cells per reaction) of the pathogen from blighted woody plant material. In testing of symptomless samples, absolute quantification of E. amylovora before and after enrichment in liquid media provided proof of E. amylovora viability and its ability to multiply, including in cases when subsequent isolation in pure culture was unsuccessful.  相似文献   

Identification and geographical distribution of pyrethroid resistance mutations in the poultry red mite Dermanyssus gallinae     

Evangelia Katsavou  Spyros Vlogiannitis  Eleanor Karp‐Tatham  Damer P Blake  Aris Ilias  Christina Strube  Ilias Kioulos  Wannes Dermauw  Thomas Van Leeuwen  John Vontas 《Pest management science》2020,76(1):125-133

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激光诊断技术在内燃机研究中的应用及其分析     

赵昌普 《拖拉机与农用运输车》2009,36(6):1-5

论述了在内燃机缸内速度测量、喷雾粒子粒度测量及燃烧过程温度和组分浓度测量中常用的激光诊断技术和测试方法,并对其工作原理、适用范围与场合、优缺点等进行了比较和分析。相位多谱勒粒子测速(PDPA)技术比较适合于单点速度、粒子尺寸及其分布的测量;粒子图像测速(PIV)技术适合于缸内二维平面速度场的测量;激光全息术是分析喷雾特性的有效途径和测量喷雾液滴尺寸的标准方法;平面激光诱导荧光(PLIF)法已成为喷雾、燃烧过程组分浓度及火焰结构研究的重要工具。  相似文献   

A Systems-Based Approach to Safeguard the Strawberry Industry from Virus Diseases     

Ioannis E. Tzanetakis 《International Journal of Fruit Science》2016,16(4):142-147

ABSTRACT

Strawberry is susceptible to several systemic pathogens and, in particular, viruses. There are several examples of disease epidemics that resulted from virus-infected propagation material and led to losses estimated in the tens of millions of dollars. This communication focuses on actions needed to minimize the risk of similar outbreaks by developing a systems-based approach from breeding to the commercial field. This includes the development of molecular biology and bioinformatic tools that allow for the detection of all known viruses infecting strawberry but most importantly for the discovery of viruses that circulate in the system undetected and may have contributed to past epidemics. Surveys in commercial fields have yielded a map of the most prevalent viruses in strawberry production areas across the United States, knowledge that will be used in the nascent National Certification Program for Strawberry.  相似文献   

Dual‐role FilmArray® diagnostics for high‐impact viral diseases          下载免费PDF全文

M Dorsch  B Meehan  WP Michalski  HG Heine  AJ Foord  G Carlile  J Wang  S McCullough  K Zuelke 《Australian veterinary journal》2016,94(3):64-66

In this study, we explored the potential utility of the human‐focused FilmArray® Respiratory Panel for the diagnosis of a broad range of influenza viruses of veterinary concern as compared with the standard portfolio of recommended TaqMan®‐based diagnostic tests. In addition, we discuss some potential operational advantages associated with the use of such integrated sample extraction, amplification and analysis devices in the context of a future long‐term, dual‐role strategy for the detection of emergency diseases of both human and veterinary concern.  相似文献   

小反刍兽疫研究进展     

徐琼  何宇乾  吴海燕 《动物医学进展》2012,(8):93-96

小反刍兽疫(PPR)是由小反刍兽疫病毒(PPRV)引起的急性或亚急性传染病,为世界动物卫生组织(OIE)法定报告的动物疫病,易感动物以山羊、绵羊等小反刍动物为主。目前,小反刍兽疫主要流行于西非、中非、中东、阿拉伯半岛及南亚等地区。敏感特异的检测方法和高效的预防性疫苗将为该病的防控奠定良好的基础。论文对小反刍兽疫全球流行现状、诊断技术及疫苗研究进展进行了综述。  相似文献   

肉毒中毒症实验室诊断的研究进展     

彭小兵 《中国兽药杂志》2009,43(3):53-57

肉毒中毒症是一种潜在的致死性瘫痪疾病,快速准确的诊断对该病的治疗显得尤为必要。本文综述了肉毒中毒症毒素检测、细菌培养、分子检测、遗传鉴定等实验室诊断的进展情况,以期对本病的快速诊断有所启示。  相似文献   

Evaluation of Epizootic Haemorrhagic Disease Virus Infection in Sentinel Calves from the San Joaquin Valley of California     

Aradaib IE  Mederos RA  Osburn BI 《Veterinary research communications》2005,29(5):447-451

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Molecular diagnostics of some trichodorid nematodes and associated Tobacco rattle virus   总被引：1，自引：1，他引：1  

K. Boutsika  M. S. Phillips  S. A. MacFarlane  D. J. F. Brown  R. C. Holeva  V. C. Blok † 《Plant pathology》2004,53(1):110-116

Several Paratrichodorus and Trichodorus (trichodorid) nematode species are natural vectors of Tobacco rattle virus (TRV) and cause economically important diseases, especially in potato and ornamental bulbous crops. Identification of trichodorid species based on morphological characters is laborious, time-consuming, and requires the services of highly trained personnel. Molecular diagnostics for trichodorid nematodes, using the ribosomal DNA repeat unit, were successfully developed to distinguish two Paratrichodorus and two Trichodorus species. The complete sequences of the 18S genes and the ITS-1 regions for these species were obtained and species-specific primers successfully designed for them. An RT-PCR assay was developed utilizing isolate-specific primers that amplify serologically distinguishable strains of TRV in individual trichodorid nematodes. The primers were based on the highly conserved RNA-1 segment of the bipartite genome and also on different parts of the RNA-2 segment of the virus genome.  相似文献