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101.
There is a general consensus that most subsidies to fisheries, particularly fuel subsidies, are harmful to both the economy and the environment. As the World Trade Organization struggles to come to an agreement on fisheries subsidies, understanding the position of the negotiating countries is crucial. As we move toward more sustainable regulations, we must ensure that no loopholes exist for countries to evade binding regulations. In this note, we use the example of the United States to illustrate how arguments for exemptions can lead to such loopholes. The United States provides interesting insight, as it appears that their support for a ban on fisheries subsidies may be contingent upon a legal pun, which would allow them and other countries to carry on subsidizing fuel to their fishing fleets into the future. 相似文献
102.
Moritz Mittelbach Andrey M Yurkov Daniele Nocentini Massimo Nepi Maximilian Weigend Dominik Begerow 《BMC ecology》2015,15(1)
Background
Studies on the diversity of yeasts in floral nectar were first carried out in the late 19th century. A narrow group of fermenting, osmophilous ascomycetes were regarded as exclusive specialists able to populate this unique and species poor environment. More recently, it became apparent that microorganisms might play an important role in the process of plant pollination. Despite the importance of these nectar dwelling yeasts, knowledge of the factors that drive their diversity and species composition is scarce.Results
In this study, we linked the frequencies of yeast species in floral nectars from various host plants on the Canary Islands to nectar traits and flower visitors. We estimated the structuring impact of pollination syndromes (nectar volume, sugar concentration and sugar composition) on yeast diversity.The observed total yeast diversity was consistent with former studies, however, the present survey yielded additional basidiomycetous yeasts in unexpectedly high numbers. Our results show these basidiomycetes are significantly associated with ornithophilous flowers. Specialized ascomycetes inhabit sucrose-dominant nectars, but are surprisingly rare in nectar dominated by monosaccharides.Conclusions
There are two conclusions from this study: (i) a shift of floral visitors towards ornithophily alters the likelihood of yeast inoculation in flowers, and (ii) low concentrated hexose-dominant nectar promotes colonization of flowers by basidiomycetes. In the studied floral system, basidiomycete yeasts are acknowledged as regular members of nectar. This challenges the current understanding that nectar is an ecological niche solely occupied by ascomycetous yeasts.Electronic supplementary material
The online version of this article (doi:10.1186/s12898-015-0036-x) contains supplementary material, which is available to authorized users. 相似文献103.
104.
Vicente Martins Gomes Rodrigo Moreira Ribeiro Alexandre Pio Viana Ricardo Moreira de Souza Eileen Azevedo Santos Daniele Lima Rodrigues Odimar Ferreira de Almeida 《European journal of plant pathology / European Foundation for Plant Pathology》2017,148(3):699-708
Interaction between the phytonematode Meloidogyne enterolobii and the fungus Fusarium solani has caused direct and indirect losses in the entire guava production chain and consequent extermination of guava plantations throughout Brazil. The combined action of these two pathogens is known as “guava decline”. In order to obtain and assess Psidium spp. interspecific hybrids for resistance to the nematode M. enterolobii, interspecific crosses of P. guineense (susceptible araçá) x P. cattleyanum (resistant araçá); P.guineense (susceptible araçá) x P. guajava (susceptible guava) and P. cattleyanum (resistant araçá) x P. guajava (susceptible guava) were conducted. These crosses resulted in hybrid immune, susceptible and resistant to Meloidogyne enterolobii. The chi-square test rejected the hypothesis of monogenic inheritance with incomplete dominance, which corroborates that this trait has polygenic action. Predictions of genetic values ??and parameters were obtained by the REML / BLUP procedure, at individual level. Finally, the 30 selected individuals (immune and resistant) were obtained, which will be backcrossed with guava for the recovery of the agronomic traits desired and subsequent release of a new cultivar. 相似文献
105.
106.
107.
Iacono E Cunto M Zambelli D Ricci F Tazzari PL Merlo B 《Veterinary research communications》2012,36(2):107-118
Domestic cats are preferred models for normal physiology and several human diseases. In the present study feline fetal fluids and membranes were evaluated as possible sources of MSCs. Samples were recovered from 4 pregnant queens after ovarian-hysterectomy. Gestational sacs were separated from uterine wall; after allantoic and amniotic fluids aspiration and chorion-allantois and amniotic membranes separation, all cell lineages were cultured into 25 cm(2) flasks, in DMEM/TCM199, in a 5% CO(2) incubator at 38.5 °C. At passage 3, chondrogenic, osteogenic and adipogenic differentiation ability were evaluated by culturing cell monolayers in differentiating media for 21 days. Cellular characterization with CD90, CD44, CD105, CD73, CD34, CD14, CD45, was performed by flow cytometry. In all samples, adherent fibroblastoid spindle-shaped cells were observed. Positive von Kossa and Alizarin Red staining confirmed osteogenesis. Alcian blue staining of matrix glycosaminoglycans illustrated chondrogenesis, and positive Oil Red O lipid droplets within cell cytoplasm suggested adipogenesis. All cell lines isolated were positive for CD90, CD44, CD105 and negative for CD34, CD14 and CD45; as unexpected and different from human cells, feline cells resulted negative for CD73. Based on this preliminary results, fetal fluids and membranes could represent an alternative sources for mesenchymal stem cells in feline species. 相似文献
108.
Massimo Saccomani Piergiorgio Stevanato Daniele Trebbi J. Mitchell McGrath Enrico Biancardi 《Euphytica》2009,169(1):19-29
Beta vulgaris genetic resources are essential for broadening genetic base of sugar beet and developing cultivars adapted to adverse environmental
conditions. Wild beets (sea beets, B. vulgaris spp. maritima and their naturalized introgressions with cultivated beets known as ruderal beets) harbor substantial genetic diversity that
could be useful for beet improvement. Here, we compared molecular and morpho-physiological traits of wild beets collected
on the Adriatic coast of Italy with sugar beet using eight primer-pairs amplifying 194 polymorphic fragments and four root
traits (glucose and fructose content in the root tip, root elongation rate, number of the of root tips, total root length
and its distribution among diameters ranges). Genetic diversity was higher in the sea beet accession, which may be due to
the highly variable selection pressures that occur in heterogeneous ecological niches, compared with the ruderal and cultivated
beets. Sea and sugar beet accessions showed contrasting root patterns in response to sulfate deprivation: sugar beet showed
an increase of reducing sugars in the root tips and higher root elongation rate, and the sea beet accession showed an increase
in root tip number, total root length and fine root length (average diameter < 0.5 mm). The ruderal beet showed intermediary
responses to sea and sugar beet accessions. AFLP and morpho-physiological cluster analyzes showed sea, ruderal and cultivated
beets to be genetically distinct groups. The results of this study indicate variability in response to sulfate deprivation
is present in undomesticated beets that could be deployed for sugar beet improvement. 相似文献
109.
110.
Stefano Demichelis Claudio Arnò Domenico Bosco Daniele Marian Piero Caciagli 《Phytoparasitica》2005,33(2):196-208
The T biotype ofBemisia tabaci (Gennadius, 1889), a population found colonizingEuphorbia characias L. plants on the Nebrodi-Peloritani mountains in Sicily, was biologically characterized. The minimum development time was
29.7 days at 28°C. Based on the regression of 1/dayvs T, the rate of development was calculated as 0.00206, the theoretical lower temperature threshold for development as 9.3°C,
and the sum of effective temperatures as 485.1. At 25° C, egg-to-adult development was significantly shorter onDatura stramonium (30.1 days) than on eitherEuphorbia pulcherrima orEuphorbia characias (35.6 and 35.4 days, respectively). The fourth instar nymphs grown onD. stramonium had the typical oval outline and seven pairs of dorsal setae located on cone-like processes, often barely visible. The fourth
instar nymphs and their pupal cases grown onE. characias had the outline deformed by the presence of hairs on the lower surface of the leaf. The pupae onD. stramonium were significantly larger (both longer and wider) than those reared onE. characias; on both host plants, female pupae were significantly larger than male ones. Analysis of variance showed that width of females
onD. stramonium was significantly larger than the width of those reared onE. characias. Attempts at courtship between T- and Q-biotypes were observed, but adults from different biotypes were never seen mating.
Only males were obtained from the seven heterologous crossing attempts, either way, whereas homologous, control breeding produced
males and females. The T biotype was able to transmitTomato yellow leaf curl Sardinia begomovirus (TYLCSV) fromD. stramonium toD. stramonium, from tomato to tomato and from tomato toD. stramonium. Attempts to transmit TYLCSV fromD. stramonium to tomato were unsuccessful. The transmission efficiency was significantly lower when tomato was the test plant. The diverse
biology and ecology of the T biotype confirm that it is genetically different from most Mediterranean biotypes.
This paper is dedicated to the dear memory of our colleague Dr Claudio Arnò, who died on March 12, 2004, while this paper
was being written. 相似文献