表达序列标签(EST)分析及其在小麦研究中的应用     

张延明  曲敏  徐香玲  李集临 《黑龙江农业科学》2007,(1):82-85

简要叙述了表达序列标签EST技术的原理,对小麦EST图谱的构建研究进展进行了综述.分别阐述了小麦七个同祖群染色体的EST图谱的研究进展,同时对EST在小麦基因组研究中的应用前景进行了展望.  相似文献   

Assessment of genotypic variation among cultivated durum wheat based on EST-SSRS and genomic SSRS   总被引：31，自引：0，他引：31  

I. Eujayl  M. Sorrells  M. Baum  P. Wolters  W. Powell 《Euphytica》2001,119(1-2):39-43

Genotypic variation of 64 durum lines, landraces, and varieties wereinvestigated using three sources of microsatellites (SSRs). 245 primer pairswere used to detect polymorphism in this collection. A total of 42polymorphic SSR primer pairs consisting of 22 EST-SSR, 11 XGWM and9 WMC were used for genotyping. The EST-SSRs primers produced highquality markers, but were least polymorphic (25%) compared to 53%for the other two sources. The 42 primers detected 189 polymorphicalleles with an average of 4.5 alleles per locus. The coefficient of similaritybetween accessions ranged from 0.28 to 0.70. The estimate of similaritydiffered when each source of SSRs was analyzed independently. This studyshowed that SSRs are highly polymorphic in durum. The data presentedhere provides a platform to develop a genotypic database for durum wheatthat will facilitate the exploitation of its genetic resources.  相似文献   

文蛤30个微卫星标记的开发及在斧文蛤和帘文蛤     

齐晓艳  董迎辉  姚韩韩  周小龙  林志华 《水产学报》2013,37(8):1147-1154

利用磁珠富集法构建的基因组文库和EST文库开发了30个文蛤多态性微卫星标记。8个G-SSR和22个EST-SSR多态性位点共获得140个等位基因,平均等位基因数为4.67,多态性信息含量、观测杂合度、期望杂合度分别介于0.172~0.744、0.040~0.720、0.187~0.793之间;G-SSRs的平均等位基因数、平均多态性信息含量、平均观测杂合度和平均期望杂合度都较EST-SSRs高;Hardy-Weinberg平衡检测发现,24个位点偏离了平衡状态;利用BLASTx对含多态性SSR位点的EST进行功能注释,11个位点来自注释基因序列。 将30对文蛤多态性SSR引物分别在斧文蛤和帘文蛤中进行了通用性检测,通用率分别为30%和40%。这些微卫星多态性位点的获得,为进一步开展文蛤遗传多样性分析、种质资源评价、分子标记辅助选育等奠定基础,并且可用于文蛤、斧文蛤和帘文蛤的比较作图、基因发掘和QTL定位等研究。  相似文献   

用EST-SSR标记分析巴西橡胶树的遗传多样性   总被引：1，自引：1，他引：0  

李维国  冯素萍  郑学项  高新生 《热带作物学报》2009,30(12):1711-1717

利用19对EST-SSRs引物对41份巴西橡胶树材料(6个野生材料和35个栽培种)进行遗传多样性分析.结果表明:19对引物均获得了预期的扩增结果,得到92个多态性位点,每对引物检测到的等位基因数为2～7个,平均为4.84个.在相似系数为0.64的水平上,野生材料和栽培种区分开来,在相似系数0.75的水平上,又可将栽培种材料分为四个类群;栽培种和野生材料间的遗传距离在0.11～1.16之间,栽培种间遗传距离较小,野生材料间遗传距离相对较大.在栽培种和野生材料之间,遗传距离最大的是AC/T/15/114与PR261(1.16),最小的是保亭155与热研7-20-59(0.11).  相似文献   

Prediction of heterosis for grain yield in rice using 'key' informative EST-SSR markers     

I. Jaikishan  P. Rajendrakumar  M. S. Ramesha    B. C. Viraktamath    S. M. Balachandran    C. N. Neeraja    K. Sujatha    K. Srinivasa Rao  P. Natarajkumar    Y. Hari    K. Sakthivel    A. S. Ramaprasad    R. M. Sundaram 《Plant Breeding》2010,129(1):108-111

This study was undertaken to assess the comparative potential of 25 Expressed Sequence Tag derived simple sequence repeats (EST-SSRs) and 25 genomic SSRs in the prediction of grain yield heterosis using a set of nine cytoplasmic male sterile (CMS) lines and 32 restorer lines of rice. EST-SSRs and genomic SSRs exhibited an average Polymorphism Information Content value of 0.37 and 0.45, respectively. The coefficient of marker polymorphism among parental lines with respect to a set of hypervariable EST and genomic SSRs was correlated with standard heterosis for grain yield of six public bred rice hybrids. EST-SSRs gave a better correlation (r = 0.75) as compared with genomic SSRs (r = 0.09). When 10 'key' informative EST-SSR markers which showed a higher positive correlation with grain yield heterosis were validated in a new set of 14 experimental hybrids, the markers exhibited a higher correlation (r = 0.79), indicating the predictive value of these EST-SSRs. We recommend these 10 'key' informative EST-SSR markers for analysis of genetic diversity of parental lines and prediction of heterosis in hybrid rice breeding programmes.  相似文献   

假臭草EST-SSRs标记的开发     

黄敏  陈振玺  刘建福  王奇志 《热带作物学报》2014,34(3):27-33

从NCBI的dbEST数据库中下载假臭草同族植物的EST序列,经EST序列处理及SSR位点查询,首次设计了27对假臭草EST-SSRs引物,以海南省文昌市假臭草DNA为模板,采用单因素和L16(45)正交试验对其EST-SSRs PCR反应体系进行优化。建立了假臭草EST-SSRs最佳20 μLPCR反应体系：Mg2+浓度为2.75 mmol/L,模板DNA用量为35 ng,Tag DNA聚合酶为2.25 U,dNTPs浓度为0.3 mmol/L,引物浓度为0.6 μmmol/L。并用14个不同来源的假臭  相似文献