Exogenous DNA Uptake of Boar Spermatozoa by a Magnetic Nanoparticle Vector System     

TS Kim  SH Lee  GT Gang  YS Lee  SU Kim  DB Koo  MY Shin  CK Park  DS Lee 《Reproduction in domestic animals》2010,45(5):e201-e206

The sperm‐mediated gene transfer method is applicable to transgenesis in many species that use spermatozoa for reproduction recently, which has been shown various results. In the current study, we show that transgenic porcine embryos can be efficiently produced by employing a simple transfection method that uses magnetic nanoparticles (MNPs). The complexes formed between plasmid DNA and MNPs were bounded on ejaculated boar spermatozoa at a higher efficiency compared to methods using DNA alone or lipofection. Using confocal microscopy, rhodamine fluorophore‐labelled MNPs were detected on external surfaces of the spermatozoa membrane, which were bounded on zona pellucida of in vitro maturated oocyte during in vitro fertilization. Electron microscopy revealed that clusters of MNPs were detected in inside of plasma membrane and nucleus of the spermatozoa head. Additionally, we found that magnetofected boar spermatozoa could be fertilized with oocytes in vitro and that the resulting gene of green fluorescent protein was detected in fertilized eggs by genomic PCR analysis. Taken together, these results suggest that MNPs can be used to efficiently introduce a transgene into embryo via spermatozoa.  相似文献   

Design of drainage culverts considering critical storm duration     

M.S. Kang  J.H. Koo  J.A. Chun  Y.G. Her  S.W. Park  K. Yoo 《Biosystems Engineering》2009,104(3):425-434

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Differential effects of treadmill exercise on calretinin immunoreactivity in type 2 diabetic rats in early and chronic diabetic stages     

Nam SM  Hwang IK  Yi SS  Yoo KY  Park OK  Yan B  Song W  Won MH  Yoon YS  Seong JK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(8):1037-1042

In this study, we investigated the effects of treadmill exercise on calretinin (CR), a marker of early postmitotic neurons, immunoreactivity in the dentate gyrus (DG) of Zucker diabetic fatty (ZDF) rats, before or after diabetes onset, and Zucker lean control (ZLC) rats. For this study, 6-week-old ZLC and prediabetic ZDF rats, and 22-week-old ZLC and ZDF rats were exercised on the treadmill. Sedentary ZLC and ZDF rats of the same age were used as exercise experiment controls. The exercised prediabetic ZDF rats did not show diabetes onset, while the sedentary prediabetic ZDF rats showed significantly increased blood glucose levels. The exercised diabetic ZDF rats exhibited a decrease in their blood glucose levels compared to the sedentary diabetic ZDF rats, but the levels were still above 20 mmol/l. ZLC rats of both ages were in the normoglycemic range. CR immunoreactivity was detected throughout the DG, including the subgranular zone and the polymorphic layer. Diabetic rats exhibited a significant decrease in the number of CR-immunoreactive cells and fibers in the DG. Exercise in the prediabetic ZDF rats significantly increased the number of CR-immunoreactive cells and fibers in the subgranular zone of the DG. In the ZLC and ZDF rats of chronic diabetic phase, exercise increased CR-immunoreactive neurons in the hilar region. These results suggest that diabetes significantly reduces the number of postmitotic CR-immunoreactive neurons and the intensity of immunoreactivity and that exercise increases these CR-related parameters in a diabetic stage-dependent manner.  相似文献   

Preparation and properties of waterborne poly(urethane-urea) ionomers effect of the type of neutralizing agent     

Jung-Eun Yang  Young-Hee Lee  Young-Seok Koo  Young-Jin Jung  Han-Do Kim 《Fibers and Polymers》2002,3(3):97-102

A series of waterborne poly(urethane-urea) anionomers were prepared from isophorone diisocyanate (IPDI), polycaprolactone diol (PCL), dimethylol propionic acid (DMPA), ethylene diamine (EDA), and triethylamine (TEA), NaOH, or Cu(COOCH₃)₂ as neutralizing agent. This study was performed to decide the effect of neutralizing agent type on the particle size, viscosity, hydrogen bonding index, adhesive strength, antistaticity, antibacterial and mechanical properties. The particle size of the dispersions decreased in the following order: TEA based samples (T-sample), NaOH based samples(N-sample), and Cu(COOCH₃)₂ based sample (C-sample). The viscosity of the dispersions increased in the order of C-sample, N-sample, and T-sample. Metal salt based film samples (N and C-sample) had much higher antistaticity than TEA based sample. By infrared spectroscopy, it was found that the hydrogen bonding index (or fraction) of samples decreased in the order of T-sample, N-sample, and C-sample. The adhesive strength and tensile modulus/strength decreased in the order of T-sample, N-sample, and C-sample. The C-sample had strong antibacterial halo, however, T- and N-samples did not.  相似文献   

Dry-jet wet spinning of polyhydroxyamide fibers     

Seung Koo Park  Seo Hyun Cho  Richard John Farris 《Fibers and Polymers》2000,1(2):92-96

A high molecular weight polyhydroxyamide (PHA) solution in N, N-dimethyl acetamide (DMAc) was prepared from 3,3′-dihydroxybenzidine and isophthalic chloride (IPC), which was used for spinning PHA fiber. Before spinning, the diffusion property of DMAc into various coagulants was examined. The fiber was well formed in coagulants such as water/ethanol with a composition of 5/5, ethanol, and ethanol/isopropanol with a composition of 7/3 and 5/5. However, the PHA fiber spun in the water/ethanol mixture contained voids. After the fiber spun in ethanol was annealed at over 350°C, the ultimate stress and initial modulus of the fiber increased from 75.5 MPa and 3.22 GPa to 369 MPa and 29.5 GPa, respectively. These properties of the PHA fiber spun by the dry spinning method were also enhanced, attaining 154 MPa and 5.56 Gpa, respectively.  相似文献   

Effects of Carbohydrate‐Rich Alternative Feedstuffs on Growth,Survival, Body Composition,Hematology, and Nonspecific Immune Response of Black Pacu,Colossoma macropomum,and Red Pacu,Piaractus brachypomus     

Rebecca Lochmann  Ruguang Chen  Fred W. Chu‐Koo  William N. Camargo  Christopher C. Kohler  Craig Kasper 《Journal of the World Aquaculture Society》2009,40(1):33-44

To facilitate economical culture of black pacu, Colossoma macropomum, and red pacu, Piaractus brachypomus, in the Amazon region of South America, we assessed locally available alternative energy sources for practical diets. We tested the effects of control diets (containing wheat products) versus diets with different Amazonian feedstuffs (yucca, Manihot sculenta, plantain, Musa paradisiaca, or pijuayo, Bactris gasipaes) on the performance of the pacus in three feeding trials. Black pacu (22.5 ± 0.03 g; Trial 1) or red pacu (2.56 ± 0.01 g; Trial 2) were fed diets containing 30% wheat bran (control) or cooked or uncooked yucca, plantain, or pijuayo for 12 wk. In Trial 3, larger black pacu (86.9 ± 6.4 g) were grown to market size in 24 wk on similar diets. Weight gain, feed conversion, survival, alternative complement activity, and lysozyme were similar among diets. Hepatosomatic index, liver glycogen, and dry matter were affected by diet in Trials 1 and 2, but effects were not consistent among trials. In Trial 3, protein efficiency ratio was lower in fish fed the diet containing wheat middlings. However, relative to wheat bran or wheat middlings, all feedstuffs tested were effective energy sources for juvenile black pacu and red pacu.  相似文献   

Influence of Annealing on Gel Properties of Mung Bean Starch     

Koo Min Chung  Tae Wha Moon  Jae Kun Chun 《Cereal Chemistry》2000,77(5):567-571

Mung bean starch gels (8% solids) were prepared after annealing at 45–60°C for 1–24 hr, and the relationship between the physical properties of gels and the swelling power (SP) and solubility of starch was investigated. The SP and solubility decreased with increasing annealing temperature and time, mostly in the first 6 hr. The solubles were mainly composed of amylose. Gel hardness at a 5 mm depth of annealed starch was larger than that of native starch, and gel hardness increased as SP decreased (r = ‐0.94). Upon continued compression, the yield force of gel showed a different function. Above SP of ≈12.5, the yield force of annealed starch gels decreased, but at <12.5 the yield force increased with increasing SP. Both granular rigidity and extent of packing appeared to determine the yield force. Although annealing increased the gel hardness, α‐amylase digestibility of gel was not affected. Pasting analysis in the Rapid Visco Analyser (RVA) revealed that annealing increased pasting temperature. A pasting peak was found only in 45 and 50°C annealed starches. Overall paste viscosities of the starches annealed at >55°C were lower than that of the control starch. Final viscosities in RVA were correlated with the yield force of gel (r = 0.99).  相似文献   

Pharmacokinetics and pharmacodynamics of intravenous esomeprazole at 2 different dosages in dogs     

Do‐Hyun Seo  Jong‐Bok Lee  Ji‐Hye Hwang  Jong‐Woo Jeong  Gun‐Ho Song  Tae‐Sung Koo  Kyoung‐Won Seo 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):531-535

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<Emphasis Type="Italic">De novo</Emphasis> assembly of potential linear artificial chromosome constructs capped with expansive telomeric repeats     

Li Lin  Dal-Hoe Koo  Wenli Zhang  Joseph St Peter  Jiming Jiang 《Plant methods》2011,7(1):10

Background  

Artificial chromosomes (ACs) are a promising next-generation vector for genetic engineering. The most common methods for developing AC constructs are to clone and combine centromeric DNA and telomeric DNA fragments into a single large DNA construct. The AC constructs developed from such methods will contain very short telomeric DNA fragments because telomeric repeats can not be stably maintained in Escherichia coli.  相似文献   

Age-related changes in calretinin-immunoreactive periglomerular cells in the rat main olfactory bulb     

Hwang IK  Yoo KY  Nam YS  Choi JH  Seo K  Lee IS  Jung JY  Kang TC  Oh YS  Won MH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(5):465-469

The changes of calretinin (CR)-immunoreactive periglomerular cells in the glomerular layer of the main olfactory bulb (MOB) were investigated in rats differing ages from postnatal month 1 (PM 1) to PM 24. The number of cresyl violet-positive periglomerular cells was similar between PM 1 and PM 12, but they decreased slightly in the PM 24 group. The size of CR-immunoreactive periglomerular cells in the glomerular layer increased with age, while their numbers did not change significantly in the PM 6-PM 24 groups. In the PM 24 group, numbers of CR-positive periglomerular cell bodies and their processes decreased, while the size of CR-positive cell bodies in the glomeruli was larger than that of the previous groups. These results suggest that CR-immunoreactive periglomerular cells in the rat MOB are well-developed in the PM 6 group, and that periglomerular cells in the PM 24 group show poor CR-immunoreactivity compared to those in the PM 6 group.  相似文献