Simple prediction of the survival of follicles in cryopreserved human ovarian tissue   总被引：2，自引：0，他引：2  

Maltaris T  Dragonas C  Hoffmann I  Mueller A  Beckmann MW  Dittrich R 《The Journal of reproduction and development》2006,52(4):577-582

This study examines the possible predictive value of the LIVE/DEAD fluorescence viability assay for evaluation of survival of cryopreserved human ovarian tissue. Ovarian tissue from ten patients was examined by LIVE/DEAD viability staining before and after cryopreservation and after freezing in a -20 C freezer (negative control). After cryopreservation with a slow freezing protocol and cryoprotectant the LIVE/DEAD assay showed 86% viable follicles (an intact oocyte and at least more than 50% of the granulosa cells alive), whereas after freezing at -20 C the survival rate was 67%. The healthy follicular loss after cryopreservation was 4%, whereas with freezing at -20 C, it was 25%. Although this assay overestimates the survival rate of cryopreserved primordial follicles, if the LIVE/DEAD assay yields greater than approximately 85% viable follicles, it can be assumed that the follicles in the cryopreserved tissue have maintained their developmental potential and that the tissue is suitable for retransplantation.  相似文献   

Notch, a universal arbiter of cell fate decisions     

Ehebauer M  Hayward P  Arias AM 《Science (New York, N.Y.)》2006,314(5804):1414-1415

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Second-harmonic generation from magnetic metamaterials     

Klein MW  Enkrich C  Wegener M  Linden S 《Science (New York, N.Y.)》2006,313(5786):502-504

We observe second-harmonic generation from metamaterials composed of split-ring resonators excited at 1.5-micrometer wavelength. Much larger signals are detected when magnetic-dipole resonances are excited, as compared with purely electric-dipole resonances. The experiments are consistent with calculations based on the magnetic component of the Lorentz force exerted on metal electrons-an intrinsic second-harmonic generation mechanism that plays no role in natural materials. This unusual mechanism becomes relevant in our work as a result of the enhancement and the orientation of the local magnetic fields associated with the magnetic-dipole resonances of the split-ring resonators.  相似文献   

Cavity cooling below the recoil limit     

M Wolke  J Klinner  H Keßler  A Hemmerich 《Science (New York, N.Y.)》2012,337(6090):75-78

Conventional laser cooling relies on repeated electronic excitations by near-resonant light, which constrains its area of application to a selected number of atomic species prepared at moderate particle densities. Optical cavities with sufficiently large Purcell factors allow for laser cooling schemes, avoiding these limitations. Here, we report on an atom-cavity system, combining a Purcell factor above 40 with a cavity bandwidth below the recoil frequency associated with the kinetic energy transfer in a single photon scattering event. This lets us access a yet-unexplored regime of atom-cavity interactions, in which the atomic motion can be manipulated by targeted dissipation with sub-recoil resolution. We demonstrate cavity-induced heating of a Bose-Einstein condensate and subsequent cooling at particle densities and temperatures incompatible with conventional laser cooling.  相似文献   

Don't look back in anger! Responsiveness to missed chances in successful and nonsuccessful aging     

Brassen S  Gamer M  Peters J  Gluth S  Büchel C 《Science (New York, N.Y.)》2012,336(6081):612-614

Life-span theories explain successful aging with an adaptive management of emotional experiences like regret. As opportunities to undo regrettable situations decline with age, a reduced engagement into these situations represents a potentially protective strategy to maintain well-being in older age. Yet, little is known about the underlying neurobiological mechanisms supporting this claim. We used a multimodal psychophysiological approach in combination with a sequential risk-taking task that induces the feeling of regret and investigated young as well as emotionally successfully and unsuccessfully (i.e., late-life depressed) aged participants. Responsiveness to regret was specifically reduced in successful aging paralleled by autonomic and frontostriatal characteristics indicating adaptive shifts in emotion regulation. Our results suggest that disengagement from regret reflects a critical resilience factor for emotional health in older age.  相似文献   

Effects of different media on proliferation and differentiation capacity of canine, equine and porcine adipose derived stem cells     

Schwarz C  Leicht U  Rothe C  Drosse I  Luibl V  Röcken M  Schieker M 《Research in veterinary science》2012,93(1):457-462

Adult stem cells are of particular interest for therapeutic use in the field of regenerative medicine. Adipose-derived mesenchymal stem cells (ASCs) are an attractive stem cell source for all fields of regenerative medicine because adipose tissue - and therewith cells - can easily be harvested from each donor. However, common expansion using fetal bovine serum (FBS) can not be used for clinical applications as xenogenic proteins must be avoided. Adipose tissue from equine, canine and porcine donors was digested with collagenase to isolate ASCs. ASCs were either expanded in a cell culture medium supplemented with FBS or in a serum-free medium (UltraCulture; UC) supplemented with a serum substitute (UltroserG). From all three animal species, the adipogenic and osteogenic differentiation potential of ASCs cultured with different media was analyzed in vitro. Cell proliferation analysis showed a population doubling time of 48-68 h for canine cells, 54-65 h for porcine cells and 54-70 h for equine cells, expanded in different media. Except for porcine ASCs, cells cultured in media supplemented with FBS grew faster than cells expanded in UC medium with UltroserG. Yet, all cells maintained their potential to differentiate into adipocytes and osteoblasts. UltraCulture medium containing UltroserG can for all examined species be recommended if FBS needs to be avoided in the expansion of donor-derived (stem) cells.  相似文献   

Tickborne encephalitis virus antibodies in wild cervids in Belgium     

Linden A  Wirtgen M  Nahayo A  Heyman P  Niedrig M  Schulze Y 《The Veterinary record》2012,170(4):108

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Does canopy mean nitrogen concentration explain variation in canopy light use efficiency across 14 contrasting forest sites?     

Peltoniemi M  Pulkkinen M  Kolari P  Duursma RA  Montagnani L  Wharton S  Lagergren F  Takagi K  Verbeeck H  Christensen T  Vesala T  Falk M  Loustau D  Mäkelä A 《Tree physiology》2012,32(2):200-218

The maximum light use efficiency (LUE?=?gross primary production (GPP)/absorbed photosynthetic photon flux density (aPPFD)) of plant canopies has been reported to vary spatially and some of this variation has previously been attributed to plant species differences. The canopy nitrogen concentration [N] can potentially explain some of this spatial variation. However, the current paradigm of the N-effect on photosynthesis is largely based on the relationship between photosynthetic capacity (A(max)) and [N], i.e., the effects of [N] on photosynthesis rates appear under high PPFD. A maximum LUE-[N] relationship, if it existed, would influence photosynthesis in the whole range of PPFD. We estimated maximum LUE for 14 eddy-covariance forest sites, examined its [N] dependency and investigated how the [N]-maximum LUE dependency could be incorporated into a GPP model. In the model, maximum LUE corresponds to LUE under optimal environmental conditions before light saturation takes place (the slope of GPP vs. PPFD under low PPFD). Maximum LUE was higher in deciduous/mixed than in coniferous sites, and correlated significantly with canopy mean [N]. Correlations between maximum LUE and canopy [N] existed regardless of daily PPFD, although we expected the correlation to disappear under low PPFD when LUE was also highest. Despite these correlations, including [N] in the model of GPP only marginally decreased the root mean squared error. Our results suggest that maximum LUE correlates linearly with canopy [N], but that a larger body of data is required before we can include this relationship into a GPP model. Gross primary production will therefore positively correlate with [N] already at low PPFD, and not only at high PPFD as is suggested by the prevailing paradigm of leaf-level A(max)-[N] relationships. This finding has consequences for modelling GPP driven by temporal changes or spatial variation in canopy [N].  相似文献   

Assessing the impact of interfering organic matter on soil metaproteomic workflow     

Matthias Waibel  Kevin McDonnell  Maria Tuohy  Sally Shirran  Sylvia Synowsky  Barry Thornton  Eric Paterson  Fiona Brennan  Florence Abram 《European Journal of Soil Science》2023,74(3):e13392

Soil organic matter (SOM) is biologically, chemically, and physically complex. As a major store of nutrients within the soil, it plays an important role in nutrient provision to plants. An enhanced understanding of SOM utilisation processes could underpin better fertiliser management for plant growth, with reduced environmental losses. Metaproteomics can allow the characterisation of protein profiles and could help gain insights into SOM microbial decomposition mechanisms. Here, we applied three different extraction methods to two soil types to recover SOM with different characteristics. Specifically, water-extractable organic matter, mineral-associated organic matter and protein-bound organic matter were targeted with the aim to investigate the metaproteome enriched in those extractions. As a proof-of-concept, replicated extracts from one soil were further analysed for peptide identification using liquid chromatography followed by tandem mass spectrometry. We employed a framework for mining mass spectra for both peptide assignment and fragmentation pattern characterisation. Different extracts were found to exhibit contrasting total protein and humic substance content for the two soils investigated. Overall, water extracts displayed the lowest humic substance content (in both soils) and the highest number of peptide identifications (in the soil investigated) with the most frequent peptide hits associated with diverse substrate/ligand binding proteins of Proteobacteria and derived taxa. Our framework also highlighted a strong peptidic signal in unassigned and unmatched spectra, information that is currently not captured by the pipelines employed in this study. Taken together, this work points to specific areas for optimisation in chromatography and mass spectrometry to adequately characterise SOM-associated metaproteomes.  相似文献   

The Rotation Problem and Tropical Deforestation in Forest Economic Textbooks: A Comparative Review     

Matthias Bösch  Martin Moog 《Journal of Sustainable Forestry》2013,32(5):512-526

In the past three decades, new trends have been influencing the field of forest economics. For example, the optimal rotation literature has focused increasingly on risk and uncertainty analysis and there has been a steady increase in the number of economic studies regarding tropical deforestation. In this article, it is investigated whether the emergence of these topics is reflected in relevant textbooks of the discipline. Moreover, some structural textbook attributes are analyzed and compared, namely the use of illustrative elements and pedagogical aids. Our results show that one can clearly recognize a steady development in the texts.  相似文献