Differential expression of molecular rust resistance components have distinctive profiles in <Emphasis Type="Italic">Coffea arabica</Emphasis> - <Emphasis Type="Italic">Hemileia vastatrix</Emphasis> interactions     

Geleta Dugassa Barka  Eveline Teixeira Caixeta  Robson Ferreira de Almeida  Samuel Mazzinghy Alvarenga  Laércio Zambolim 《European journal of plant pathology / European Foundation for Plant Pathology》2017,149(3):543-561

Countering the economic hurdle caused by coffee leaf rust disease is most appealing at this time as it has posed a major threat to coffee production around the world. Establishing differential expression profiles at different times following pathogen invasion in both innate and acquired immunities unlocks the molecular components of resistance and susceptibility. Suppression subtractive hybridization (SSH) was used to identify genes differentially over-expressed and repressed during incompatible and compatible interactions between Coffea arabica and Hemileia vastatrix. From 433 clones of expressed sequence tags (ESTs) sequenced, 352 were annotated and categorized of which the proportion of genes expressed during compatible interaction were relatively smaller. The result showed upregulation and downregulation of various genes at 12 and 24 h after pathogen inoculation in both interactions. The use of four different databases in searching for gene homology resulted in different number of similar sequences. BLASTx against EMBL-EBI (European Molecular Biology Laboratory-European Bioinformatics Institute) database being with the maximum (100%) hits for all the annotated sequences. RT-qPCR analysis of seven resistance-signaling genes showed similar expression patterns for most of the genes in both interactions, indicating these genes are involved in basal (non-specific) defense during which immune reactions are similar. Using SSH, we identified different types of resistance related genes that could be used for further studies towards resistant cultivar development. The potential role of some of the resistance related proteins found were also discussed.  相似文献   

Glaucoma with Descemet's membrane detachment in five horses          下载免费PDF全文

Michala de Linde Henriksen  Noelle La Croix  David A. Wilkie  Mary Lassaline‐Utter  Karen R. Brantman  Gillian L. Beamer  Leandro B. C. Teixeira  Richard R. Dubielzig 《Veterinary ophthalmology》2017,20(3):273-279

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Patient characteristics influencing the variability of distributed parameter‐based models in DCE‐CT kinetic analysis          下载免费PDF全文

M. D. La Fontaine  L. S. McDaniel  L. N. Kubicek  R. J. Chappell  L. J. Forrest  R. Jeraj 《Veterinary and comparative oncology》2017,15(1):105-117

Kinetic parameter variability may be sensitive to kinetic model choice, kinetic model implementation or patient‐specific effects. The purpose of this study was to assess their impact on the variability of dynamic contrast‐enhanced computed tomography (DCE‐CT) kinetic parameters. A total of 11 canine patients with sinonasal tumours received high signal‐to‐noise ratio, test‐double retest DCE‐CT scans. The variability for three distributed parameter (DP)‐based models was assessed by analysis of variance. Mixed‐effects modelling evaluated patient‐specific effects. Inter‐model variability (CV_inter) was comparable to or lower than intra‐model variability (CV_intra) for blood flow (CV_inter:[4–28%], CV_intra:[28–31%]), fractional vascular volume (CV_inter:[3–17%], CV_intra:[16–19%]) and permeability‐surface area product (CV_inter:[5–12%], CV_intra:[14–15%]). The kinetic models were significantly (P<0.05) impacted by patient characteristics for patient size, area underneath the curve of the artery and of the tumour. In conclusion, DP‐based models demonstrated good agreement with similar differences between models and scans. However, high variability in the kinetic parameters and their sensitivity to patient size may limit certain quantitative applications.  相似文献   

Inheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene     

Giovani Greigh de Brito  Eveline Teixeira Caixeta  Ana Paula Gallina  Eunize Maciel Zambolim  Laércio Zambolim  Valdir Diola  Marcelo Elhers Loureiro 《Euphytica》2010,173(2):255-264

The most important disease of Coffea arabica is coffee leaf rust caused by the fungus Hemileia vastatrix. The purpose of this study was to characterize the inheritance of coffee resistance gene(s) to race II of this pathogen and to identify and map molecular markers linked to this trait. Different populations were used: F₂ (160 plants), BCr (20), and BCs (135), derived from a cross between the resistant genotype Híbrido de Timor UFV 427-15 and the susceptible cultivar Catuaí Amarelo UFV 2143-236 (IAC 30). The segregation analysis showed that the resistance of Híbrido de Timor to race II of the H. vastatrix is conferred by a single dominant gene. The amplification of 176 AFLP (Amplified fragment length polymorphism) primer combinations using bulked segregant analysis (BSA) allowed the identification of three molecular markers linked to the resistance gene. Genetic mapping of these three markers in the F₂ population indicated that they are distributed on both sides, flanking the resistance gene. The markers E.CTC/M.TTT405 and E.CGT/M.TGT300 were found linked to the resistance gene at 8.69 cM (LOD 18.91) and 25.10 cM (LOD 5.37), respectively, while E.CCT/M.TTC230 was localized on the other side of the gene, at 20.50 cM (LOD 6.15). These markers are the first rust resistance markers identified in Híbrido de Timor and can be useful for marker assisted selection in coffee breeding programs.  相似文献   

Carbon dioxide fluxes across the Sierra de Guadarrama, Spain     

R. Inclán  C. Uribe  D. De La Torre  D. M. Sánchez  M. A. Clavero  A. M. Fernández  R. Morante  A. Cardeña  M. Fernández  A. Rubio 《European Journal of Forest Research》2010,129(1):93-100

Understanding the spatial and temporal variation in soil respiration within small geographic areas is essential to accurately assess the carbon budget on a global scale. In this study, we investigated the factors controlling soil respiration in an altitudinal gradient in a southern Mediterranean mixed pine–oak forest ecosystem in the north face of the Sierra de Guadarrama in Spain. Soil respiration was measured in five Pinus sylvestris L. plots over a period of 1 year by means of a closed dynamic system (LI-COR 6400). Soil temperature and water content were measured at the same time as soil respiration. Other soil physico-chemical and microbiological properties were measured during the study. Measured soil respiration ranged from 6.8 to 1.4 μmol m^?2 s^?1, showing the highest values at plots situated at higher elevation. Q ₁₀ values ranged between 1.30 and 2.04, while R ₁₀ values ranged between 2.0 and 3.6. The results indicate that the seasonal variation of soil respiration was mainly controlled by soil temperature and moisture. Among sites, soil carbon and nitrogen stocks regulate soil respiration in addition to soil temperature and moisture. Our results suggest that application of standard models to estimate soil respiration for small geographic areas may not be adequate unless other factors are considered in addition to soil temperature.  相似文献   

Molecular detection of Bartonella henselae and Bartonella clarridgeiae in clinical samples of pet cats from Southern Italy     

M.G. Pennisi  E. La Camera  L. Giacobbe  B.M. Orlandella  V. Lentini  S. Zummo  M.T. Fera 《Research in veterinary science》2010,88(3):379-384

Bartonella henselae is considered an emerging pathogen of veterinary and medical interest that can be occasionally transmitted to humans. Cats are considered to be the only reservoir host for B. henselae. In this study, we used a nested-PCR assay to investigate the prevalence of B. henselae and Bartonella clarridgeiae DNA in peripheral blood samples, fine needle lymph node aspirate specimens and oral swabs from 85 cats in order to develop an easy diagnostic strategy for the selection of infection-free cats that are being considered as pets, especially for immunocompromised patients. Overall, molecular analysis showed that 71 cats (83.5%) tested PCR positive for the presence of B. henselae DNA. PCR amplification of DNA B. henselae produced positive products from lymph node aspirate specimens (62/85; 72.9%) similar to those obtained from blood samples (60/85; 70.6%) and higher than those from oral swabs (51/85; 60%) of cats. No PCR product was obtained for B. clarridgeiae. The simultaneous analysis of three different clinical samples in our study increased the diagnostic possibilities for B. henselae infection in the examined cats from 60–72.9% to 83.5%. Lymph node aspirates were found to be the most effective clinical samples for the detection of B. henselae and blood samples were the next best. Oral swab samples were used in this study with good results when considered in combination with blood and/or lymph node aspiration. The use of nested-PCR assay on these three clinical samples may enhance the diagnostic sensitivity for bartonellosis in cats irrespective of the clinical status of animals.  相似文献   

Retraction     

Beloqui A  Guazzaroni ME  Pazos F  Vieites JM  Godoy M  Golyshina OV  Chernikova TN  Waliczek A  Silva-Rocha R  Al-Ramahi Y  La Cono V  Mendez C  Salas JA  Solano R  Yakimov MM  Timmis KN  Golyshin PN  Ferrer M 《Science (New York, N.Y.)》2010,330(6006):912

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利用GnRH+PGF2α进行绵羊同期发情的效果观察     

杨梅  汪立琴  陈童  郭志勤  郝根  莎拉  郭立 《黑龙江动物繁殖》2010,18(2):14-15

利用99只绵羊,在繁殖季节,采用促排卵3号+PG诱导同期发情的试验。结果表明,72h内的发情率达100%,排卵率在90%以上;对86只受体羊的黄体侧子宫角移植两枚经玻璃化冷冻-解冻后的胚胎,其总受胎率为38.37%。  相似文献   

青海高原日光温室秋冬茬西芹种植技术     

喇成寿 《农业科技通讯》2010,(8):194-195

西芹与我县传统种植的本地芹相比,具有药味淡、纤维含量低、脆嫩、耐寒、适应性强、产量高等特点,而且生长旺盛、叶柄变大、营养丰实,有降压、健脑、清肠、利便的作用。  相似文献   

Aporphine alkaloids,clerodane diterpenes,and other constituents from Tinospora cordifolia     

Phan Van Kiem  Chau Van Minh  Nguyen Tien Dat  La Van Kinh  Dan Thuy Hang  Nguyen Hoai Nam  Nguyen Xuan Cuong  Hoang Thanh Huong  Trinh Van Lau 《Fitoterapia》2010

Phytochemical investigation of the methanol extract of Tinospora cordifolia aerial parts led to the isolation of four new and seven known compounds. The structures of two new aporphine alkaloids, N-formylasimilobine 2-O-β-d-glucopyranosyl-(1 → 2)-β-d-glucopyranoside (tinoscorside A, 1) and N-acetylasimilobine 2-O-β-d-glucopyranosyl-(1 → 2)-β-d-glucopyranoside (tinoscorside B, 2), a new clerodane diterpene, tinoscorside C (3), and a new phenylpropanoid, sinapyl 4-O-β-d-apiofuranosyl-(1 → 6)-O-β-d-glucopyranoside (tinoscorside D, 6) were determined by extensive spectroscopic methods including FTICR-MS and 1D and 2D NMR.  相似文献