木薯MePP2CAa基因克隆、表达及蛋白互作分析          下载免费PDF全文

曾坚  李丽珍  沈梓欣  林墁  刘博婷  吴春来  李冰  胡伟  曾力旺 《华中农业大学学报》2024,43(1):141-148

为探究2C型蛋白磷酸酶（protein phosphatase 2C， PP2C）在木薯响应非生物胁迫过程中的作用，利用木薯Arg7叶片cDNA扩增MePP2CAa基因，分析该基因序列、启动子活性、不同逆境和激素处理下的表达模式以及与ABA受体PYLs之间的互作关系。序列分析结果显示，MePP2CAa基因全长1 311 bp，编码436个氨基酸，具有PP2C家族的结构域特征，与橡胶树和麻风树的PP2C序列同源性最高，分别为78.95%和74.09%，在C端保守；qRT-PCR分析结果显示，MePP2CAa基因在木薯储藏根中的表达显著高于茎、叶中的表达量；不同逆境和激素处理结果显示，甘露醇、NaCl、ABA、MeJA、低温和SA处理可以显著诱导MePP2CAa基因的表达；MePP2CAa基因启动子序列分析显示，启动子包含ABA应答元件（abscisic acid responsive element，ABRE）、MeJA响应元件、干旱诱导元件等；酵母双杂交结果显示MePP2CAa能够与MePYL1互作。以上结果表明，MePP2CAa基因可能响应木薯的非生物胁迫。  相似文献   

正交试验法优化黄褐毛忍冬总皂苷的提取工艺     

缪艳燕  张永萍 《安徽农业科学》2010,38(11):5872-5873

[目的]建立黄褐毛忍冬（Lonicera fulvotomentosa Hsu et S.C.Cheng）总皂苷的水提工艺。[方法]以黄褐毛忍冬总皂苷中有效成分α-常春藤皂苷的含量为考察指标,采用正交设计法考察加水量、煎煮时间、煎煮次数及浸泡时间4个因素对黄褐毛忍冬总皂苷提取工艺的影响,同时采用高效液相法测定α-常春藤皂苷的含量。[结果]加水量、煎煮次数、煎煮时间、浸泡时间对α-常春藤皂苷含量的影响程度不同,各因素影响程度的大小为：煎煮次数〉加水量〉浸泡时间〉煎煮时间,即煎煮次数对煎煮条件影响最大,为最重要的因素,其次为加水量、浸泡时间、煎煮时间。最优方案为：黄褐毛忍冬药材不用浸泡,加10倍量水煎煮3次,每次1.0h。方差分析结果表明,加水量和煎煮次数对α-常春藤皂苷含量的影响具有显著意义（PA=0.034〈0.05,PB=0.020〈0.05）,而煎煮时间、浸泡时间对水α-常春藤皂苷含量无显著影响（PC=0.500〉0.05,PD=0.264〉0.05）,对测定结果影响小,这与直观分析结果相吻合。[结论]优选提取工艺简单、稳定、可行。  相似文献   

基于ARM9内核的智能灌溉控制系统的设计     

戴春霞  赵德安 《安徽农业科学》2010,38(15):8208-8210

介绍了基于ARM920T内核的嵌入式控制系统的设计与实施,并将控制系统引入设施栽培自动灌溉控制中。实践证明,该系统的设计达到了预期的效果。  相似文献   

Resveratrol Downregulates Cyp2e1 and Attenuates Chemically Induced Hepatocarcinogenesis in SD Rats     

Xiongfei Wu  Chenggang Li  Guozhen Xing  Xinming Qi  Jin Ren 《Journal of toxicologic pathology》2013,26(4):385-392

Cyp2e1 plays an important role in chemically induced hepatocarcinogenesis. Resveratrol (REV) is known to prevent diethylnitrosamine (DEN)-induced hepatocarcinogenesis, but its effects on this process induced by DEN and 2-acetylaminofluorene (2-AAF) and the role of Cyp2e1 remain unclear. In this study, glutathione S-transferase placental form (GST-P)-positive foci were used as a marker of hepatocarcinogenesis. REV or diallyl disulfide (DADS, an inhibitor of Cyp2e1) significantly reduced both the area and number of GST-P-positive foci induced by DEN and 2-AAF. Treatment with REV or DADS also markedly decreased the expression of Cyp2e1 in the rat liver. By immunohistochemical staining of serial liver sections, we found that the expression of Cyp2e1 in GST-P-positive foci showed three distinct patterns: decreased in GST-P foci, increased in GST-P foci when compared with surrounding liver tissue and mixed type. The number of GST-P foci with increased Cyp2e1 expression was greater than the number of GST-P foci with decreased Cyp2e1. Protein levels of GST-P and Cyp2e1 were also higher in foci compared with surrounding liver tissue. REV or DADS significantly reduced the expression of GST-P and Cyp2e1 in both foci and surrounding liver tissue. Taken together, these results suggested that REV has a significant inhibitory effect on chemically induced hepatocarcinogenesis, which may be attributed to downregulation of Cyp2e1.  相似文献   

鸡β-防御素-2基因的克隆及原核表达载体的构建     

王海英  祁克宗  彭开松  孙洁 《水禽世界》2008,(3):6-9

鸡β-防御素是一类广谱抗菌阳离子小肽的总称,一般具有3个分子内二硫键。防御素对细菌、真菌乃至某些被膜病毒具有广谱杀伤作用。试验根据已发表的Gal-2基因序列,设计并合成了一对引物。以骨髓中的总RNA为模板,利用RT-PCR技术扩增目的片段并克隆到pGEM-TEasy载体上,经筛选鉴定获得阳性重组子pGEM-T-Gal-2。再经EcoRI、XbaI双酶切,插入pMAL-c2x质粒构建表达载体。利用PCR技术和酶切反应筛选转化子成功获得pMAL-c2x-Gal-2重组质粒。  相似文献   

网络课件在C程序设计语言教学中的应用   总被引：1，自引：0，他引：1  

黄海鸥  曲琳琳 《吉林农业科技学院学报》2008,17(4):110-111

传统的黑白教学方法存在着一些弊端，导致学习效率降低、资源浪费。网络教学这种新型教育模式不仅大大提高了学习效率，而且很大程度地发挥了教育资源的作用。本文以C程序设计语言为例，阐述了制作网络课件的关键技术和实现技巧。  相似文献   

水温对怀头鲇血液学指标的影响     

支兵杰  刘伟  王立波  段莹莹  陈军 《水产学杂志》2008,21(2)

本实验设定4℃、9℃、14℃、18℃、24℃和30℃6个水温梯度，测定了水温变化对二龄怀头鲇血液生理生化指标的影响。结果显示，水温4～30℃范围内，怀头鲇血红蛋白含量随着水温的升高呈波浪型变化，但变化幅度较小；而血沉与血红蛋白含量变化趋势相反，变化幅度较大；血液中的红细胞数量随水温的升高先增加再减少；血清中的总蛋白、白蛋白和球蛋白的变化趋势一致，都是随水温的升高其含量先增加再减少，而且在9～18℃区间变化较急剧；血清中的血糖，高密度脂蛋白，谷草转氨酶，三碘甲状腺氨酸，甲状腺素，促甲状腺激素，钾和镁八项指标在各水温之间不存在显著差异（P〉0．05）；血清中的尿素氮，甘油三脂，总胆固醇，低密度脂蛋白，谷丙转氨酶，碱性磷酸酶，钠，钙，氯化物和渗透压在部分水温之间存在显著差异（P〈0．05）。  相似文献   

The effects of compost in a rice–wheat cropping system on aggregate size,carbon and nitrogen content of the size–density fraction and chemical composition of soil organic matter,as shown by 13C CP NMR spectroscopy     

QIUJUN WANG  QIWEI HUANG  LI ZHANG  JIANCHAO ZHANG  QIRONG SHEN  WEI RAN 《Soil Use and Management》2012,28(3):337-346

We investigated whether the long‐term application of compost from agricultural waste improved soil physical structure, fertility and soil organic matter (SOM) storage. In 2006, we began a long‐term field experiment based on a rice–wheat rotation cropping system, having a control without fertilizer (NF) and three treatments: chemical fertilizers (CF), pig manure compost (PMC) and a prilled mixture of PMC and inorganic fertilizers (OICF). Following the harvest of wheat in 2010, the mean‐weight diameter (MWD) of water‐stable aggregates and the concentration of C and N in bulk soil (0–20 cm; <2 mm fraction) were significantly greater (P < 0.05) in PMC and NF plots than in CF or OICF plots. Pig manure compost significantly increased the proportion of >5‐mm aggregates, whereas CF significantly increased the proportion of 0.45‐ to 1‐mm aggregates. The C and N contents of all density fractions were greater in PMC than in other treatments with levels decreasing in the following order: free particulate organic matter (fPOM) >occluded particulate organic matter (oPOM) > mineral‐combined SOM (mineral–SOM). Solid‐state ¹³C CPMAS NMR spectra showed that alkyl C/O‐alkyl C ratios and aromatic component levels of SOM were smaller in PMC and OICF plots than in CF plots, suggesting that SOM in PMC and OICF plots was less degraded than that in CF plots. Nevertheless, yields of wheat in PMC and NF plots were smaller than those in CF and OICF plots, indicating that conditions for producing large grain yields did not maintain soil fertility.  相似文献   

浅析灵石县污水处理厂污水处理工艺     

温彩芳 《绿色科技》2012,(8):133-134,137

以灵石县污水处理厂污水处理工艺为例,分析了A2/O工艺和BAF工艺原理、特点及存在的问题,为一些污水处理厂污水处理工艺的选择提供参考。  相似文献   

产蛋鸡日粮中添加Vc的效果试验     

张文举  谭守仁 《青海畜牧兽医杂志》1998,28(4):15-16

选取海兰商品蛋鸡１０８只，分为对照和试验Ⅰ、Ⅱ３组，每组３６只，分别饲喂添加０、１００、２００ｍｇ／ｋｇＶｃ的蛋鸡日粮。４２天的试验结果：Ⅰ组提高蛋鸡产蛋数６．３６％，饲料转化效率提高６．７％，经济效益增加１７．０２％；Ⅱ组降低蛋鸡产蛋数１．４５％，降低饲料转化效率２．９４％，比对照组少收入６．３％。说明适宜的Ｖｃ添加量能显著提高产蛋鸡的生产性能。  相似文献