Use of an antineoepitope antibody for identification of type-II collagen degradation in equine articular cartilage.     

R C Billinghurst  E M Buxton  M G Edwards  M S McGraw  C W McIlwraith 《American journal of veterinary research》2001,62(7):1031-1039

OBJECTIVE: To develop an antibody that specifically recognizes collagenase-cleaved type-II collagen in equine articular cartilage. SAMPLE POPULATION: Cartilage specimens from horses euthanatized for problems unrelated to the musculoskeletal system. PROCEDURE: A peptide was synthesized representing the carboxy- (C-) terminus (neoepitope) of the equine type-II collagen fragment created by mammalian collagenases. This peptide was used to produce a polyclonal antibody, characterized by western analysis for reactivity to native and collagenase-cleaved equine collagens. The antibody was evaluated as an antineoepitope antibody by ELISA, using peptides +/- an amino acid at the C-terminus of the immunizing peptide. Collagen cleavage was assayed from equine articular cartilage cultured with interleukin-1 (IL-1), +/- a synthetic MMP inhibitor, BAY 12-9566. Cartilage specimens from osteoarthritic and nonarthritic joints were compared for antibody staining. RESULTS: An antibody, 234CEQ, recognized only collagenase-generated 3/4-length fragments of equine type-II collagen. This was a true antineoepitope antibody, as altering the C-terminus of the immunizing peptide significantly decreased competition for binding in an inhibition ELISA. The IL-1-induced release of type-II collagen fragments from articular cartilage was prevented with the MMP inhibitor. Cartilage from an osteoarthritic joint of a horse had increased staining with the 234CEQ antibody, compared with normal articular cartilage. CONCLUSIONS AND CLINICAL RELEVANCE: We generated an antineoepitope antibody recognizing collagenase-cleaved type-II collagen of horses. This antibody detects increases in type-II collagen cleavage in diseased equine articular cartilage. The 234CEQ antibody has the potential to aid in the early diagnosis of arthritis and to monitor treatment responses.  相似文献   

HHMI Awards     

Buxton IL 《Science (New York, N.Y.)》1996,271(5251):895a

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A Preliminary Ichthyoplankton Survey of the Tsitsikamma National Park     

R.L. Tilney  C.D. Buxton 《African Zoology》2013,48(3):204-211

An ichthyoplankton survey using bongo nets was conducted within the Tsitsikamma National Park over an 18-month period between January 1991 and July 1992. Four sampling stations along the coast were chosen, approximately 5 km apart. At each station four samples were taken along a transect perpendicular to the coast, between 0,35 km and 3,8 km offshore. Horizontal tows were taken at three and 15 m below the surface over reef and sand substrata respectively. Larvae belonging to 24 of the 35 families and 55 of the 97 species known to occur in the area were captured. Engraulid larvae completely dominated the catches while amongst the reef-associated taxa sparids and blenniids were prominent. Seasonally occurring cold upwelling events impacted negatively on egg and larval abundance. Mean values for egg and larval abundances were 1207 and 282 per 100 m³ respectively. A relationship between larval number and plankton volume was revealed. Most larvae captured were between 2,0 and 20,3 mm (SL), and in the preflexion condition. The absence of larger size classes in samples was attributed to net avoidance.  相似文献   

Continuous infusion of propofol in calves: bispectral index and hemodynamic effects          下载免费PDF全文

Mauricio Deschk  Juliana T Wagatsuma  Marcelo A Araújo  Guilherme GF Santos  Sergio SR Júnior  Caio JX Abimussi  Carlos E Siqueira  Jéssica CL Motta  Silvia HV Perri  Paulo SP Santos 《Veterinary anaesthesia and analgesia》2016,43(3):309-315

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Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations     

Antoine LF Gady  Freddy WK Hermans  Marion HBJ Van de Wal  Eibertus N van Loo  Richard GF Visser  Christian WB Bachem 《Plant methods》2009,5(1):13-14

Background  

The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as a model for berry-fruit plants. With the progress of the tomato sequencing project, reverse genetics becomes an obvious and achievable goal.  相似文献   

Effect of Leptin on In Vivo Goat Embryo Production     

AM Batista  WA Gomes  CCD Carvalho  PLJ Monteiro Jr  FLM Silva  FC Almeida  PC Soares  GF Carneiro  MMP Guerra 《Reproduction in domestic animals》2014,49(3):476-480

The aim of this study was to evaluate the effect of leptin administration during superovulation on in vivo goat embryo production. Ten mature does were superovulated with 133 mg follicle‐stimulating hormone (FSH) i.m. in six descending doses at 12‐h intervals. The goats received 4.8 μg/kg human recombinant leptin s.c. (leptin group, n = 5) or phosphate‐buffered saline (PBS) (control group, n = 5) with the first and second FSH doses. The does were mated and subjected to embryo collection by transcervical technique 6 days later. The total number of cells per embryo and the number of cells with fragmented DNA were assessed in selected blastocysts by combining Hoechst 33342 and terminal dUTP nick‐end labelling (TUNEL) staining. Plasma concentrations of oestradiol (E₂) and progesterone (P₄) were determined by electrochemiluminescence from the day of FSH treatment, on the day of superovulatory oestrus and on the day before embryo collection. Compared with the control group, the does that received leptin had a higher number of transferable embryos (p < 0.005), fewer embryos classified as degenerated (p < 0.001) and fewer TUNEL‐positive cells/blastocyst (p < 0.001). The number of transferable embryos was positively correlated with E₂ concentrations on day of oestrus (r = 0.562; p < 0.01) and P₄ concentrations on the day of embryo collection (r = 0.912; p < 0.001). We concluded that in vivo leptin administration during FSH treatment improved embryo quality and affected ovarian steroidogenesis in superovulated goats.  相似文献   

Immunization against 5α-androstenone in boarsL'immunisation contre la 5-androsténone chez le verratImmunisierung gegen 5α-Androstenon in Ebern     

E.D Williamson  R.L.S Patterson  E.R Buxton  K.G Mitchell  I.G Partridge  N Walker 《Livestock Production Science》1985,12(3):251-264

The effects of specific immunization against 5α-androstenone have been examined in large, genetically homologous groups of boars reared either to bacon weight (90–95 kg live weight) or to heavy manufacturing weight (115–120 kg live weight). At the lighter weight, immunization significantly (P < 0.05) reduced the concentration of androstenone in the adipose tissue from a mean value of 1.77 (S.E. 0.2) μg g^?1 fat in untreated boars (n=39) to 1.10 (S.E. 0.18) μg g^?1 for animals (n=19) treated with 5α-androstene-3-BSA. In contrast, boars (n=20) treated with 5α-androstenone-11-BSA as immunogen accumulated androstenone to a level of 1.99 μg g^?1 fat (S.E. 0.38). At the heavier weight, immunization reduced the accumulation of androstenone in adipose tissue from a mean value of 1.81 μg g^?1 fat (S.E. 0.22) in untreated boars (n=76) to 1.17 μg g^?1 (S.E. 0.19) for animals (n=22) treated with androstene-3-BSA as immunogen. In contrast, boars (n=21) treated with androstenone-11-BSA as immunogen accumulated androstenone to a mean level of 1.74 μg g^?1 fat (S.E. 0.46). No detrimental side-effects were observed in the immunized animals and the advantages of male-type performance and carcass composition were fully preserved.  相似文献   

ADDITIONAL PEA VARIETIES AND NEW SELECTIONS RESISTANT TO FUSARIUM WILT   总被引：1，自引：1，他引：0  

E. W. Buxton  D. A. Doling  J. D. Reynolds 《Plant pathology》1960,9(2):54-56

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Antibody to alcelaphine herpesvirus-1 (AHV-1) in hamsters experimentally infected with AHV-1 and the 'sheep-associated' agent of malignant catarrhal fever   总被引：2，自引：0，他引：2  

H W Reid  I Pow  D Buxton 《Research in veterinary science》1989,47(3):383-386

Malignant catarrhal fever was induced in four groups of hamsters by the inoculation of cells infected with either the C/500 isolate of alcelaphine herpes-virus-1 (AHV-1) or the sheep-associated agent derived from cattle, red deer or Père David's deer. Using an indirect immunofluorescence assay, antibody to AHV-1 was detected in sera of clinically affected animals of all four groups. The reaction of sera from hamsters affected with malignant catarrhal fever induced by AHV-1 caused diffuse cytoplasmic staining while that from sera of hamsters with the sheep-associated form of the disease stained particulate nuclear antigens. Tests employing three other bovid herpesviruses were negative and no reaction was found with sera from normal hamsters. These studies provide convincing evidence that a virus antigenically related to AHV-1 is the cause of sheep-associated malignant catarrhal fever and that the same virus probably causes this form of the disease in both cattle and deer.  相似文献   

Titration of Toxoplasma gondii oocysts in non-pregnant sheep and the effects of subsequent challenge during pregnancy     

C McColgan  D Buxton  D A Blewett 《The Veterinary record》1988,123(18):467-470

Fifty-nine ewes, seronegative to Toxoplasma gondii, were allocated to four groups which received 2000, 200, 20 or no M1 strain toxoplasma oocysts 56 days before mating. Fifty-one of them subsequently became pregnant and were challenged with 10,000 oocysts between 78 and 83 days of gestation. Infection with 2000 oocysts induced a pyrexia, seroconversion and protective immunity in all the recipient animals. Ewes that received either 20 or no oocysts before pregnancy were susceptible to subsequent challenge and severe fetal mortality occurred. In this study 200 oocysts was the threshold value for the induction of toxoplasma infection in sheep, although not all the ewes that seroconverted to this dose were protected against further challenge.  相似文献