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1.
Emmanouil Tzimtzimis Maria Kouki Stefania Rampidi Matina Giannikaki Georgia Karnezi Lysimachos G. Papazoglou 《The Canadian veterinary journal. La revue veterinaire canadienne》2016,57(5):507-510
Duodenal and jejunal resections were performed in a cat with septic peritonitis due to small intestinal perforations by a linear foreign body. Three days later jejunal resection and anastomosis were repeated due to dehiscence of the anastomosis site. This segment of intestine was exteriorized through the body wall and managed with bandages for 5 days before it was surgically replaced into the abdomen. The cat made a full recovery. 相似文献
2.
We investigated the mechanisms of resistance to α-cypermethrin in a Q biotype, highly resistant Bemisia tabaci strain (GRMAL-RP) isolated from Crete. Cytochrome P450-dependent monoxygenase activity with the substrate ethoxycoumarin, and carboxylesterase activity with the substrates α-naphthyl-acetate, β-naphthyl-acetate, and para-nitrophenol acetate were substantially elevated in the GRMAL-RP, compared to the susceptible SUD-S strain, while glutathione-S-transferase activity with the substrate 1-chloro-2,4-dinitrobenzene was not different. The metabolic inhibitors piperonyl butoxide and S,S,S-tributyl phosphorotrithioate synergised cypermethrin toxicity in the GRMAL-RP strain, however, mortality was still lower than that of the susceptible strain, indicating the presence of an additional resistance mechanism. Analysis of the sequence of the IIS4-IIS6 region of the para sodium channel gene of the GRMAL-RP strain revealed two amino acid replacements compared to that of the SUD-S susceptible strain. One is the leucine to isoleucine substitution at position 925 (L925I) previously implicated in B. tabaci pyrethroid resistance and the other is a novel kdr resistant mutation for B. tabaci, a threonine to valine substitution at position 929 (T929V). Genotype analysis showed that the L925I and T929V were present in all GRMAL-RP males tested, at an approximately 1:1 frequency, but never in combination in the same haplotype. 相似文献
3.
Desneux Nicolas Han Peng Mansour Ramzi Arn&#; Judit Br&#;vault Thierry Campos Mateus R. Chailleux Anais Guedes Raul N. C. Karimi Javad Konan Kouassi Arthur J. Lavoir Anne-violette Luna Mar&#;a G. Perez-Hedo Meritxell Urbaneja Alberto Verheggen Fran&#;ois J. Zappal&#; Lucia Abbes Khaled Ali Abid Bayram Yunus Cantor Fernando Cuthbertson Andrew G. S. De Vis Raf Erler Fedai Firake Dnyaneshwar M. Haddi Khalid Hajjar M. Jamal Ismoilov Khasan Jaworski Coline C. Kenis Marc Liu Hao-tian Madadi Hossein Martin Thibaud Mazih Ahmed J. Messelink Gerben Mohamed Samira A. Nofemela Robert S. Oke Abiola Ramos C&#;sar Ricupero Michele Roditakis Emmanouil Shashank Pathour R. Wan Fang-Hao Wang Ming-hui Wang Su Zhang Yi-Bo Biondi Antonio 《Journal of pest science》2022,95(1):17-39
Journal of Pest Science - The South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), has invaded most Afro-Eurasian countries and is threatening worldwide tomato... 相似文献
4.
Dermitzakis ET 《Science (New York, N.Y.)》2011,331(6018):689-690
5.
Determination of intracellular cytokines IFN-γ and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture 
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下载免费PDF全文 Emmanouil I. Papadogiannakis Vasilios I. Kontos Maria Tamamidou Anastasia Roumeliotou 《Canadian journal of veterinary research》2009,73(2):137-143
This report describes a whole-blood flow cytometric method for the determination of intracellular cytokines IFN-γ and IL-4 in canine T lymphocyte subpopulations. Conjugated anti-cytokine antibodies and commercially available reagents for cell fixation and permeabilization were used. Canine peripheral blood was cultured with a combination of phorbol-12-myristate-13-acetate (PMA) and ionomycin to promote cytokine synthesis in each cell, along with monensin to increase the sensitivity of the method by retaining IFN-γ and IL-4 within the cell to detectable levels. The optimum concentrations of PMA and ionomycin were determined. Maximum IFN-γ expression from both CD4+ and CD8+ T lymphocytes was detected after 6 h of incubation of cell culture, while maximum IL-4 production took 6 h from CD4+ cells and 4 h from CD8+ cells. This method is a simple immunologic technique for measuring intracellular cytokines which could be of value in the investigation of canine immunological response mainly in various intracellular and extracellular infections, since IFN-γ and IL-4 are considered key cytokines activating the cellular and humoral immunity, respectively. 相似文献
6.
Emmanouil A. Markakis Georgios C. Koubouris Chrysi K. Sergentani Eleftherios K. Ligoxigakis 《European journal of plant pathology / European Foundation for Plant Pathology》2017,149(2):277-283
In the present study, four Greek (Agiorgitiko, Asyrtiko, Roditis and Xinomavro) and one international (Soultanina) grapevine cultivars (Vitis vinifera L.) were screened for their resistance to Phaeomoniella chlamydospora. Artificial inoculation was carried out by drilling a hole into the trunk and injecting a concentrated conidial suspension into the vessels. Disease reactions were evaluated in an 87-day assessment period, on the basis of external symptoms (disease incidence, disease severity and mortality) and by calculating the relative areas under disease progress curves (relative AUDPC). The extension of vascular browning as well as the isolation ratio along the inoculated vine trunks were also taken into account as additional parameters for evaluating resistance. The results indicated that the resistance of grapevine cultivars to P. chlamydospora varied significantly. ‘Agiorgitiko’ and ‘Soultanina’ were susceptible, whereas ‘Asyrtiko’ and ‘Xinomavro’ were resistant; ‘Roditis’ showed an intermediate level of resistance. Cultivars’ resistance was mostly distinguished in terms of the extension of vascular browning and pathogen isolation ratio. On the contrary, the disease incidence, final disease severity, mortality and relative AUDPC provided less distinctive efficiency in resistance evaluation. The robust methodology presented here could be useful in rapid evaluation experiments for future screening programs to search and recognize natural resistant sources within grapevine genotypes against P. chlamydospora. 相似文献
7.
Emmanouil Roditakis Maria Grispou Evangelia Morou Jon Bent Kristoffersen Nikos Roditakis Ralf Nauen John Vontas Anastasia Tsagkarakou 《Pest management science》2009,65(3):313-322
BACKGROUND: A major problem of crop protection in Crete, Greece, is the control of Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) with chemical insecticides owing to the rapid development of resistance. The aim of this study was to investigate the establishment of resistance and the underlying mechanisms to major insecticide classes with classical bioassays and known biochemical resistance markers. RESULTS: During a 2005–2007 survey, 53 Q biotype populations were collected. Application history records showed extensive use of neonicotinoids, organophosphates, carbamates and pyrethroids. High resistance levels were identified in the majority of populations (>80%) for imidacloprid (RF: 38–1958×) and α‐cypermethrin (RF: 30–600×). Low resistance levels (RF < 12) were observed for pirimiphos‐methyl. A strong correlation between resistance to imidacloprid and the number of applications with neonicotinoids was observed. Significant correlations were observed between COE and P450‐dependent monoxygenase activity with resistance to α‐cypermethrin and imidacloprid respectively. A propoxur‐based AChE diagnostic test indicated that iAChE was widespread in most populations. Resistance levels for α‐cypermethrin were increased when compared with a previous survey (2002–2003). Differentiation of LC50 values between localities was observed for imidacloprid only. CONCLUSION: Bemisia tabaci resistance evolved differently in each of the three insecticides studied. Imidacloprid resistance seems less established and less persistent than α‐cypermethrin resistance. The low resistance levels for pirimiphos‐methyl suggest absence of cross‐resistance with other organophosphates or carbamates used. Copyright © 2008 Society of Chemical Industry 相似文献
8.
Ioannis T. Karapanagiotidis Pier Psofakis Elena Mente Emmanouil Malandrakis Eleni Golomazou 《Aquaculture Nutrition》2019,25(1):3-14
Two feeding trials examined the replacement of fishmeal (FM) with poultry by‐product meal (PBM) in the diet of juvenile Sparus aurata. In Feeding trial I (100 days), three diets were formulated, where FM protein was replaced by 50% (PBM50) and 100% (PBM100) PBM, while in Feeding trial II (110 days), four diets were formulated using the same FM control diet, but FM was replaced at lower levels: 25% (PBM25), and 25% (PBM25 + ) and 50% (PBM50 + ) with the supplementation of lysine and methionine amino acids. PBM protein can successfully replace 50% of FM protein in the diet of S. aurata without adverse effects on survival, feed intake, growth performance and feed utilization, given that the diet is balanced with lysine and methionine. The proximate composition of body and muscle was unaffected by the diet, but the total FM replacement resulted in reduced lipid and energy contents in fish. A fifty per cent FM replacement by PBM did not affect haematological parameters indicating a good fish health. Similarities in trypsin and chymotrypsin activities with FM‐fed fish suggest a high digestibility of PBM. High dietary levels of PBM reduced the liver gene expression of GH/IGF axis and of cathepsin D suppressing fish growth and modulating the protein turnover. 相似文献
9.
The evolution of multiple‐insecticide resistance in UK populations of tomato leafminer,Tuta absoluta
10.
The resistance levels to alpha-cypermethrin, bifenthrin, pirimiphos-methyl, endosulfan and imidacloprid were determined in Bemisia tabaci (Gennadius) from Crete. Five B tabaci populations collected from greenhouse and outdoor crops were bioassayed and compared with a reference susceptible strain. Bemisia tabaci collected in a floriculture greenhouse exhibited the highest resistance against all insecticides: at LC50, resistance factors were 23-fold for bifenthrin, 80-fold for alpha-cypermethrin, 18-fold for pirimiphos-methyl, 58-fold for endosulfan and 730-fold for imidacloprid. A population collected on outdoor melons was more susceptible than the reference strain against all insecticides tested, suggesting the occurrence of local highly susceptible B tabaci populations in 'refugia'. In pairwise comparisons of resistance levels, correlation was observed between the LC50 values of the pyrethroid insecticides bifenthrin and alpha-cypermethrin. 相似文献