Phylogenetic relationships of rodent pinworms (genus Syphacia) in Japan inferred from mitochondrial CO1 gene sequences     

Okamoto M  Urushima H  Iwasa M  Hasegawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(5):545-547

Species of the genus Syphacia are considered to have generally co-evolved with their rodent hosts. This study determined partial sequences of the CO1 gene from several species in the genus Syphacia and discuss the relationships between pinworms and their hosts. Syphacia montana, which parasitizes Microtinae, was closely related to S. frederici and S. obvelata, which parasitize Murinae. Although both S. obvelata and S. ohtaorum parasitize rodents in the genus Mus, these two species were not found to be closely related to each other. Syphacia frederici, S. emileromani and S. agraria are all pinworms of the Apodemus species, but genetic affiliation between these three species was not indicated. These facts suggest that the co-evolutionary relationship between species of the genus Syphacia and their host rodents may not so strict and host switching has probably occurred during the course of evolution.  相似文献   

A pleomorphic adenoma of the lacrimal gland in a dog   总被引：1，自引：0，他引：1  

Hirayama K  Kagawa Y  Tsuzuki K  Kotani T  Azuma Y  Yoshino T  Taniyama H 《Veterinary pathology》2000,37(4):353-356

A 13-year-old female mongrel dog had a pleomorphic adenoma of the lacrimal gland in the right upper orbit. The tumor measured 3.8 x 3.0 x 3.3 cm, appeared white, round, and firm, and pressed the right globe and surrounding tissues. Histopathologically, the tumor had a thin connective tissue capsule and was composed of tubules with two cell types, some resembling luminal epithelial cells making up the tubular structures and the other of myoepithelial cells. Epithelial tubules were disposed in an adenomatous fashion and separated from each other by proliferating pleomorphic myoepithelial cells. Immunohistochemically, large numbers of the luminal epithelial cells revealed an immunopositive reaction against keratin/cytokeratin (AE1/AE3), and some epithelial cells reacted against cytokeratin 14. Spindle-shaped myoepithelial cells revealed an immunopositive reaction against cytokeratin 14, alpha-smooth muscle actin, and vimentin. A small number of myoepithelial cells reacted against desmin. S-100 protein immunopositivity was frequently found in luminal epithelial cells and rarely in the pleomorphic myoepithelial cells. Glial fibrillary acidic protein positivity was commonly found in myoepithelial cells, myxoid matrices, and intracystic materials, but not in luminal epithelial cells.  相似文献   

Ophiostomatoid fungi associated with bark beetles onAbies veitchii in wave-regenerated forests     

Nobuaki Ohtaka  Hayato Masuya  Shigeru Kaneko  Yuichi Yamaoka  Masashi Ohsawa 《Journal of Forest Research》2002,7(3):145-151

Relationships between tree mortality and bark beetle infestation onAbies veitchii at the wave-regenerated forest in Mt. Asahi, Okuchichibu area were investigated. Most of the firs with green needles and newly developed current year’s shoots in the dieback zone were heavily infested by bark beetles before the death of the trees. After heavy infestation of beetles, about half of the infested firs died within the year, and the other half died in the next year. When the species composition of bark beetles and associated ophiostomatoid fungi were investigated in Mt. Asahi and also at a typical wave-regenerated forest in Mt. Shimagare, Yatsugatake area,Cryphalus montanus andC. piceae were dominant beetle species for Mt. Asahi and Mt. Shimagare, respectively.Ophiostoma subalpinum andO. europhioides were dominant fungal species at both wave-generated forests. BecauseO. subalpinum was detected more frequently from deeper areas of sapwood thanO. europhioides, it was suggested that the fungal species may accelerate the death of stressed firs in wave-regenerated forests. Contribution No. 169, Laboratory of Plant Parasitic Mycology, Institute of Agriculture and Forestry, University of Tsukuba.  相似文献   

Comparison of stand dynamics after dieback caused by pine wilt disease among pine forests with different management regimes in western Japan     

Keiji?SakamotoEmail author  Naoko?Miki  Taiyo?Tsuzuki  Takashi?Nishimoto  Ken?Yoshikawa 《Journal of Forest Research》2003,8(4):303-309

In pine forests damaged by pine wilt disease, in western Japan, the effect of protection regimes of pine trees on the stand dynamics were examined in the following four stands: (1) lightly damaged stand (age 30–40 years) with no procedure in operation for protecting pine trees; (2) severely damaged stand (age 30–40 years) with no procedure in place for protecting pine trees; (3) severely damaged stand (age 50 years) with a selective cutting of infected trees; (4) severely damaged stand (age 30–40 years) with a selective cutting of infected trees. All the stands had been abandoned before the pine wilt disease damage. The understory structure of the severely damaged stand with no protection procedure was similar to that of the lightly damaged stand. Frequent invasion by tree species and acceleration in the growth of understory trees occurred after the dieback in the selective cutting stand. These results suggest that a deficiency in the canopy layer caused by the dieback resulted in low disturbance intensity in the early stages after the dieback, but the selective cutting increased the intensity by the reduction in the understory as well as the canopy layer. The intensity of the disturbance in the selective cutting stands was larger in the younger stand because it had a higher density of selectively cut pine trees. The different stand structure of pine forests occurred after the dieback because the intensity of the disturbance varied as a result of the selective cutting operation and the stand age.  相似文献   

Comparison of the canine corneal epithelial cell sheets cultivated from limbal stem cells on canine amniotic membrane,atelocollagen gel,and temperature‐responsive culture dish          下载免费PDF全文

Eunryel Nam  Naoki Fujita  Maresuke Morita  Keiko Tsuzuki  Hsing Yi Lin  Cheng Shu Chung  Takayuki Nakagawa  Ryohei Nishimura 《Veterinary ophthalmology》2015,18(4):317-325

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Genomic Reassortants of Pandemic A (H1N1) 2009 Virus and Endemic Porcine H1 and H3 Viruses in Swine in Japan     

Rikio KIRISAWA  Yoshitaka OGASAWARA  Hayato YOSHITAKE  Asuka KODA  Tokujiro FURUYA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(11):1457-1470

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Ionomic Profiling of Rice Genotypes and Identification of Varieties with Elemental Covariation Effects     

Zhang Chengming  Nobuhiro Tanaka  Maria Stefanie Dwiyanti  Matthew Shenton  Hayato Maruyama  Takuro Shinano  Chu Qingnan  Xie Jun  Toshihiro Watanabe 《水稻科学》2022,29(1):76-88

Ionomic profiles are primarily influenced by genetic and environmental factors. Identifying ionomic responses to varietal effects is necessary to understand the ionomic variations among species or subspecies and to potentially understand genetic effects on ionomic profiles. We cultivated 120 rice (Oryza sativa) varieties to seedling stage in identical hydroponic conditions and determined the concentrations of 26 elements (including 3 anions) in the shoots and roots of rice. Although the subspecies effects were limited by the genus Oryza pre-framework and its elemental chemical properties, we found significant differences in ionomic variations in most elements among the aus, indica and japonica subspecies. Principal component analysis of the correlations indicated that variations in the root-to-shoot ionomic transport mechanisms were the main causes of ionomic differences among the subspecies. Furthermore, the correlations were primarily associated with the screening of varieties for elemental covariation effects that can facilitate breeding biofortified rice varieties with safe concentrations of otherwise toxic elements. The japonica subspecies exhibited the strongest elemental correlations and elemental covariation effects, therefore, they showed greater advantages for biofortification than the indica and aus subspecies, whereas indica and aus subspecies were likely safer in metal(loid) polluted soils. We also found that geographical and historical distribution significantly defined the ionomic profiles. Overall, the results of this study provided a reference for further association studies to improve the nutritional status and minimize toxicity risks in rice production.  相似文献   

Initiating and cancer-propagating cells in TEL-AML1-associated childhood leukemia     

Hong D  Gupta R  Ancliff P  Atzberger A  Brown J  Soneji S  Green J  Colman S  Piacibello W  Buckle V  Tsuzuki S  Greaves M  Enver T 《Science (New York, N.Y.)》2008,319(5861):336-339

Understanding cancer pathogenesis requires knowledge of not only the specific contributory genetic mutations but also the cellular framework in which they arise and function. Here we explore the clonal evolution of a form of childhood precursor-B cell acute lymphoblastic leukemia that is characterized by a chromosomal translocation generating a TEL-AML1 fusion gene. We identify a cell compartment in leukemic children that can propagate leukemia when transplanted in mice. By studying a monochorionic twin pair, one preleukemic and one with frank leukemia, we establish the lineal relationship between these "cancer-propagating" cells and the preleukemic cell in which the TEL-AML1 fusion first arises or has functional impact. Analysis of TEL-AML1-transduced cord blood cells suggests that TEL-AML1 functions as a first-hit mutation by endowing this preleukemic cell with altered self-renewal and survival properties.  相似文献   

Enterotoxigenicity of heat-resistant and heat-sensitive strains of Clostridium perfringens     

H Sunagawa  T Tsuzuki  K Takeshi  Y Ando  S Oka 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1987,49(5):853-858

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Effects of photoperiod on salsolinol‐induced prolactin secretion in goats     

Tomoyoshi YAEGASHI  Jin JIN  Tatsuru SAWADA  Hayato SAITO  Ferenc FÜLÖP  György Miklos NAGY  Tsutomu HASHIZUME 《Animal Science Journal》2012,83(5):418-425

The aim of the present study was to clarify the relation between salsolinol (SAL)‐induced prolactin (PRL) release and photoperiod in goats. A single intravenous (i.v.) injection of SAL was given to adult female goats under short (8 h light, 16 h dark) or long (16 h light, 8 h dark) photoperiod conditions at two different ambient temperatures (20°C or 5°C), and the PRL‐releasing response to SAL was compared to that of thyrotropin‐releasing hormone (TRH) or a dopamine (DA) receptor antagonist, sulpiride. SAL, as well as TRH or sulpiride, stimulated the release of PRL promptly after each injection in both 8‐ and 16‐h daily photoperiods at 20°C (P < 0.05). The area under the response curve (AUC) of PRL for the 60‐min period after injections of saline (controls), SAL, TRH and sulpiride in the 16‐h daily photoperiod group was greater than each corresponding value in the 8‐h daily photoperiod group (P < 0.05). There were no significant differences in the AUC of PRL among the values produced after the injection of SAL, TRH and sulpiride in 16‐h daily photoperiod group; however, the values produced after the injection of TRH were smallest among the three in the 8‐h daily photoperiod group (P < 0.05). The PRL‐releasing responses to SAL, TRH and sulpiride under a short and long photoperiod condition at 5°C resembled those at 20°C. These results show that a long photoperiod highly enhances the PRL‐releasing response to SAL as well as TRH or sulpiride in either medium or low ambient temperature in goats.  相似文献