Identification of internal control genes in milk‐derived mammary epithelial cells during lactation cycle of Indian zebu cow          下载免费PDF全文

Pradeep Jatav  Monika Sodhi  Ankita Sharma  Sandeep Mann  Amit Kishore  Umesh K Shandilya  Ashok K Mohanty  Ranjit S Kataria  Poonam Yadav  Preeti Verma  Surinder Kumar  Dhruba Malakar  Manishi Mukesh 《Animal Science Journal》2016,87(3):344-353

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Papaya yellow leaf curl virus: A newly identified begomovirus infecting Carica papaya L. from the Indian Subcontinent     

Chitra Nehra  Avinash Marwal  Rakesh Kumar Verma  Megha Mishra  Pradeep Sharma 《The Journal of Horticultural Science and Biotechnology》2019,94(4):475-480

India is one of the world’s largest producers of papaya. Viruses, mainly begomoviruses and potyviruses, cause a significant loss in papaya production. The study described here has identified a new species of begomovirus and a new species of betasatellite infecting Carica papaya in India. The sequences of the begomovirus and betasatellite show 90.03% nucleotide sequence identity to an isolate of Radish leaf curl virus and 92.25% identity to an isolate of Tomato leaf curl betasatellite, respectively. Maximum likelihood phylogenetic tree of begomovirus sequence of isolate DP2 (KX353622) showed distant relationships with previously characterised begomoviruses. Recombination analysis proposed six recombination breakpoints in begomovirus genome with other geographical begomovirus isolates.  相似文献   

Segmentation of 3D images of plant tissues at multiple scales using the level set method     

Annamária Kiss  Typhaine Moreau  Vincent Mirabet  Cerasela Iliana Calugaru  Arezki Boudaoud  Pradeep Das 《Plant methods》2017,13(1):114

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Nursery rearing of kalbasu, Labeo calbasu (Hamilton), at different stocking densities in outdoor concrete tanks     

Pradeep Kumar Sahu  Joykrushna Jena  & Pratap Chandra Das 《Aquaculture Research》2007,38(2):188-192

The growth and survival of kalbasu, Labeo calbasu, was evaluated at stocking densities of 5, 10 and 15 million spawn ha⁻¹ in nursery rearing in concrete tanks of 50 m², each for a period of 25 days. Survival of fry was density dependent and significantly higher (54.5%) at 5 million ha⁻¹ than those at 10 million ha⁻¹ (50.1%) and 15 million ha⁻¹ (46.9%). Similarly, growth and specific growth rate were inversely related to the stocking density and varied significantly among the three densities.  相似文献   

Effects of Polyether Ionophores on the Protective Immune Responses of Broiler Chickens against Angara Disease and Newcastle Disease Viruses     

Munir K  Muneer MA  Tiwari A  Chaudhry RM  Muruganandan S 《Veterinary research communications》2007,31(7):909-929

Immunization against Angara disease virus (ADV), a serotype 4 avian adenovirus, and Newcastle disease virus (NDV), an avian paramyxovirus serotype 1, is the mainstay of a broiler vaccination programme, while polyether ionophores usually form an essential component of a broiler medication programme in most parts of India and Pakistan. The role of polyether ionophores in the protective immune responses of broiler chickens vaccinated and challenged with ADV and NDV was investigated. A total of 1600 birds were divided into eight groups of 200 birds each. First four groups were vaccinated against NDV and ADV, while the remaining four served as unvaccinated controls. The first 3 groups of birds were administered salinomycin, monensin and cyclophosphamide (CYP), respectively. The last group served as an untreated control. The same treatment schedule was also followed for the next four unvaccinated groups. The post-vaccination and post-challenge serological responses to NDV and ADV, body and lymphoid organ weight gains, post-challenge survival rate and detection of NDV and ADV in the tissues of infected birds were evaluated. Birds administered salinomycin showed a significant stimulation of protective immune responses against both NDV and ADV as compared to the untreated and CYP-treated birds. Monensin also enhanced the protective immune responses against both viruses but the effect was not statistically significant. Thus, it is concluded that monensin and salinomycin augment the anti-NDV and anti-ADV immune responses in broiler chickens, which supports their use in poultry flocks.  相似文献   

Rice-wheat productivity and nutrient status in a lantana- (Lantana spp.) amended soil     

Pradeep K. Sharma  J. K. Ladha  T. S. Verma  R. M. Bhagat  A. T. Padre 《Biology and Fertility of Soils》2003,37(2):108-114

Imbalanced and inadequate use of chemical fertilizers is responsible for low rice- (Oryza sativa L.) wheat (Triticum aestivum L.) productivity in many resource-poor farmers' fields. Wheat yields in post-rice soils are also constrained due to soil conditions created by puddling in rice, especially in fine to medium textured soils. Organic amendments are known to improve soil productivity under rice-wheat cropping by way of improving physical conditions and nutrient status of the soil, but their availability is restricted. There is a need to identify locally available and cost-effective organic materials, which have minimal alternate uses as fodder and fuel. We evaluated lantana (Lantana spp. L.) residues, a fast-growing weed in nearby wastelands, as a potential soil organic amendment. Yield trends, and soil and crop nutrient status in a 12-year rice-wheat experiment at Palampur, India, involving four levels (0, 10, 20, and 30 Mg ha^-1 year^-1 fresh mass) of lantana addition were investigated. Chopped lantana was incorporated into soil 10–15 days before puddling. Lantana additions at 10, 20 and 30 Mg ha^-1 increased rice yields on average by 18%, 23% and 30%, wheat yields by 11%, 14% and 20%, and total system productivity (rice + wheat) by 15%, 20% and 26% over controls, respectively, and at the same time saved NPK fertilizer. Linear regression analyses over 12 years did not show any change in yield trends of rice and wheat at P =0.05. Continuous cultivation of rice-wheat significantly increased total C, labile C, and other C indices of soils. Total N, Olsen's P, and NH₄OAc-extractable K in the lantana-amended plots were higher than in the controls. Nutrient concentrations in crop biomass, however, remained generally unaffected by lantana treatments. Results suggest that lantana residues, which improved the nutrient status of soil and system yield, have the potential for resource conservation and sustaining rice-wheat productivity.  相似文献   

Comment on "Arsenic Mobility and Groundwater Extraction in Bangladesh" (I)     

Aggarwal PK  Basu AR  Kulkarni KM 《Science (New York, N.Y.)》2003,300(5619):584; author reply 584

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Effect of long-term manuring and fertilizers on carbon pools,soil structure,and sustainability under different cropping systems in wet-temperate zone of northwest Himalayas     

Sudhir Verma  Pradeep Kumar Sharma 《Biology and Fertility of Soils》2007,44(1):235-240

We investigated C management index (CMI; an indicator of sustainability of a management system and is based on total and labile C) and soil aggregation in medium-textured soils (silt loam and silty clay loam) under different cropping systems as follows: maize-wheat (M-W), rice-wheat (R-W), soybean-wheat (S-W), Guinea grass, and Setaria grass. Field experiments were 6–32 years long and were located in the wet-temperate zone of northwest Himalayas. The plant nutrients were applied through chemical fertilizers (urea, superphosphate, and muriate of potash) with or without organic materials (FYM, wheat straw, and Lantana spp.). The content of total C (C_T), labile C (C_L), CMI, mean weight diameter (MWD), and aggregate porosity varied significantly under different cropping systems. The range was 1.59 (R-W)–4.29% (Setaria) for C_T, 1.23 (R-W)–3.89 mg/kg (Guinea grass) for C_L, 52.09 (R-W)–129.77 (Guinea grass) for CMI, 0.90 (R-W)–5.09 (Guinea grass) for MWD, and 41.5 (R-W)–56.8% (S-W) for aggregate porosity. Aggregate porosity was highest (56.8%) under S-W, followed by grasses (50.1–51.2%), and M/R-W (41.5–50.0%). As per these data, (a) continuous use of N alone as urea lowered soil sustainability over control (no fertilizers); (b) use of NPK at recommended rates improved soil productivity over control; (c) the NPK + organic amendments further improved soil sustainability; and (d) the sustainability under different cropping systems followed the order: perennial grasses > soybean-wheat > maize-wheat > rice-wheat.  相似文献   

Uptake and bioconcentration of dieldrin,dimethoate, and permethrin by Tetrahymena pyriformis     

Pradeep Bhatnagar  Surendar Kumar  Rup Lal 《Water, air, and soil pollution》1988,40(3-4):345-349

A ciliate protozoan, Tetrahymenapyriformis was exposed to three insecticides, dieldrin, dimethoate, and permethrin for 12 hr to study the uptake and bioconcentration potential. Ciliates concentrated 922, 3547, and 1056 gg g^?1 dry wt. over an initial concentration of 1 gg mL^?1 of dieldrin, dimethoate, and permethrin, respectively. The highest bioconcentration factor for three insecticides was 2095, 3547, and 1110, respectively. It is suggested that if levels in the environment reach 1 gg mL^?1 the chief effects would be reduction of cell population, and accumulation of the toxicants by ciliates. Accumulation of insecticides by ciliates would permit the toxicants to enter aquatic food chains. Thus the compounds could exert toxic effects at higher trophic levels.  相似文献   

The epidemiology of Fascioliasis in Malawi: I. The epidemiology in the intermediate host     

S. A. T. Mzembe  M. A. Chaudhry 《Tropical animal health and production》1979,11(1):246-250

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