Stem cell factor supports migration in canine mesenchymal stem cells     

Nathaly Enciso  Luciana L. K. Ostronoff  Guillermo Mejías  Leticia G. León  María Luisa Fermín  Elena Merino  Cristina Fragio  Luis Avedillo  Concepción Tejero 《Veterinary research communications》2018,42(1):29-38

Adult Mesenchymal Stem Cells (MSC) are cells that can be defined as multipotent cells able to differentiate into diverse lineages, under appropriate conditions. These cells have been widely used in regenerative medicine, both in preclinical and clinical settings. Initially discovered in bone marrow, MSC can now be isolated from a wide spectrum of adult and foetal tissues. Studies to evaluate the therapeutic potential of these cells are based on their ability to arrive to damaged tissues. In this paper we have done a comparative study analyzing proliferation, surface markers and OCT4, SOX9, RUNX2, PPARG genes expression in MSC cells from Bone marrow (BMMSC) and Adipose tissue (ASC). We also analyzed the role of Stem Cell Factor (SCF) on MSC proliferation and on ASCs metalloproteinases MMP-2, MMP-9 secretion. Healthy dogs were used as BMMSC donors, and ASC were collected from omentum during elective ovariohysterectomy surgery. Both cell types were cultured in IMDM medium with or without SCF, 10% Dog Serum (DS), and incubated at 38 °C with 5% CO2. Growth of BMMSCs and ASCs was exponential until 25–30 days. Flow citometry of MSCs revealed positive results for CD90 and negative for CD34, CD45 and MCH-II. Genes were evaluated by RT-PCR and metalloproteinases by zymografy. Our findings indicate morphological and immunological similarities as well as expression of genes from both origins on analyzed cells. Furthermore, SCF did not affect proliferation of MSCs, however it up-regulated MMP-2 and MMP-9 secretion in ASCs. These results suggest that metalloproteinases are possibly essential molecules pivoting migration.  相似文献   

Heritability and genetic correlations between rumination time and production traits in Holstein dairy cows during different lactation phases          下载免费PDF全文

Riccardo Moretti  Marcos Paulo Gonçalves de Rezende  Stefano Biffani  Riccardo Bozzi 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2018,135(4):293-299

So far, rumination has been used as a proxy for monitoring dairy cow health at farm level. However, investigating its genetic aspects as well as its correlation with other important productive traits may turn this management tool into a new informative selection criterion. However, scientific evidences on genetic correlation among rumination time (RT) and milk production and milk composition are still scarce. Therefore, the objective of this study was to estimate the heritability of RT across three lactation phases and its genetic correlation with milk production, milk composition and somatic cell count (SCC). Results of our study showed that heritability for RT was 0.34 and was constant across lactation. The mean genetic correlations between RT and milk production and composition traits were 0.07 (milk production), ?0.07 (protein yield), ?0.31 (fat yield), and ?0.32 (fat/protein ratio). The mean genetic correlation between RT and the SCC was 0.05.  相似文献   

Flash flood simulation and valve behavior of Mytilus galloprovincialis measured with Hall sensors     

Piero ADDIS  Alberto ANGIONI  Viviana PASQUINI  Angelica GIGLIOLI  Valeria ANDREOTTI  Stefano CARBONI  Marco SECCI 《Integrative zoology》2021,16(2):138-148

Mussels close their shell as a protective strategy and the quantification of this behavioral marker may represent an alarm signal when they are exposed to environmental stressors. In the present study, we investigated the ability of the Mediterranean mussel Mytilus galloprovincialis to recover and then the resilience or inertia of valve activity after a pulsing exposition to diverse levels of salinity (5, 10, 20, and 35 PSU as reference value). The trial simulated an event of drastic and sudden reduction of seawater salinity thus mimicking an event of flash flood from intense rain. Valve gaping and movements were measured in continuous cycle for 10 days using a customized magneto-electric device which uses Hall sensors. Results showed that under normal conditions of salinity (35 PSU), the general pattern of valve movements was a continuously open state with sporadic spikes indicating a closing motion. At salinity of 5, PSU mussels reacted by closing their valves, leading to a 77% mortality on the 4th day. At salinity of 10, PSU animals were observed with closed valves for the entire duration of the exposure and no mortality occurred, they showed a significant reduction in the valve activity once the reference value of salinity was re-established. In contrast, salinity of 20 PSU did not trigger a significant behavioral response. Interestingly, there no define rhythms of valve movements were recorded during salinity challenges.  相似文献   

Herd-level prevalence and risk factors for bovine viral diarrhea virus infection in cattle in the State of Paraíba,Northeastern Brazil     

Leise Gomes Fernandes  Adriana Hellmeister de Campos Nogueira  Eliana De Stefano  Edviges Maristela Pituco  Cláudia Pestana Ribeiro  Clebert José Alves  Tainara Sombra Oliveira  Inácio José Clementino  Sérgio Santos de Azevedo 《Tropical animal health and production》2016,48(1):157-165

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Detection of bacterial contamination and DNA quantification in stored blood units in 2 veterinary hospital blood banks          下载免费PDF全文

Valentina Stefanetti  Arianna Miglio  Katia Cappelli  Stefano Capomaccio  Elisa Sgariglia  Maria L. Marenzoni  Maria T. Antognoni  Mauro Coletti  Vittorio Mangili  Fabrizio Passamonti 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(3):406-410

Blood transfusions in veterinary medicine have become increasingly more common and are now an integral part of lifesaving and advanced treatment in small and large animals. Important risks associated with transfusion of blood products include the transmission of various infectious diseases. Several guidelines suggest what infectious agents to screen for in canine and feline transfusion medicine. However, while the risk of bacterial contamination of blood products during storage and administration has not been documented in veterinary medicine, it has emerged as a cause of morbidity and mortality in human transfusion medicine. Clinical experience shows that the majority of blood component bacterial contaminations are caused by only a few species. Unlike other types of bacteria, psychrotolerant species like Pseudomonas spp. and Serratia spp. can proliferate during the storage of blood units at 4°C from a very low titer at the time of blood collection to a clinically significant level (> 10⁵ CFU/mL) causing clinical sepsis resulting from red blood cell concentrate transfusions in human medicine. The purpose of this report was to describe the detection and quantification procedures applied in 4 cases of bacterial contamination of canine and feline blood units, which suggest the need for further investigations to optimize patients’ safety in veterinary transfusion medicine.  相似文献   

Feeding preference and intraguild interactions between the parasitoid Trichogramma achaeae and the predator Macrolophus pygmaeus,two biological agents of Tuta absoluta     

Isabel Borges  Luisa Oliveira  Ana Durão  Patricia Arruda  António O. Soares 《Pest management science》2023,79(11):4376-4382

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Thyroidectomy in dogs with thyroid tumors: Survival analysis in 144 cases (1994-2018)     

Daniela Enache  Livia Ferro  Emanuela M. Morello  Federico Massari  Giorgio Romanelli  Stefano Nicoli  Stefano Guazzetti  Federico Porporato  Eric Zini 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(2):635-647

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Bioactive Compounds in Wild Asteraceae Edible Plants Consumed in the Mediterranean Diet     

Panfili  Gianfranco  Niro  Serena  Bufano  Annarita  D’Agostino  Annacristina  Fratianni  Alessandra  Paura  Bruno  Falasca  Luisa  Cinquanta  Luciano 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(4):540-546

Plant Foods for Human Nutrition - Three wild edible plant species belonging to the Asteraceae family, Crepis vesicaria L. (s.l.), Sonchus asper (L.) Hill s.l., and Sonchus oleraceus L., usually...  相似文献   

Exogenous Salicylic Acid Improves Phenolic Content and Antioxidant Activity in Table Grapes     

Blanch  Gracia Patricia  Gómez-Jiménez  Maria C.  del Castillo  Maria Luisa Ruiz 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(2):177-183

Plant Foods for Human Nutrition - Grapes contain high contents of phenolics, which are known to possess health promoting properties. Exogenous application of phytoregulators, mainly methyl...  相似文献   

Toward the Identification of Novel Antimicrobial Agents: One-Pot Synthesis of Lipophilic Conjugates of N-Alkyl d- and l-Iminosugars     

Anna Esposito  Daniele D&#x;Alonzo  Stefano D&#x;Errico  Eliana De Gregorio  Annalisa Guaragna 《Marine drugs》2020,18(11)

In the effort to improve the antimicrobial activity of iminosugars, we report the synthesis of lipophilic iminosugars 10a–b and 11a–b based on the one-pot conjugation of both enantiomeric forms of N-butyldeoxynojirimycin (NBDNJ) and N-nonyloxypentyldeoxynojirimycin (NPDNJ) with cholesterol and a succinic acid model linker. The conjugation reaction was tuned using the established PS-TPP/I₂/ImH activating system, which provided the desired compounds in high yields (94–96%) by a one-pot procedure. The substantial increase in the lipophilicity of 10a–b and 11a–b is supposed to improve internalization within the bacterial cell, thereby potentially leading to enhanced antimicrobial properties. However, assays are currently hampered by solubility problems; therefore, alternative administration strategies will need to be devised.  相似文献