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The marine leech, Zeylanicobdella arugamensis, is a major threat to aquaculture in grouper-producing countries including Indonesia. This study aimed at investigating prevalence, intensity and histopathology of the ectoparasite in humpback and hybrid groupers cultured in different rearing systems. A total of 260 groupers (60 humpback groupers and 200 hybrid groupers) were used for samples. The marine leech was observed on skin, fins, gills and mouth, followed by histopathological assay on the skin tissue. The results showed that prevalence of the leech in both groupers was higher when they were cultured in the floating net cages compared with the hatchery, p < .05. Furthermore, humpback grouper had a higher prevalence than hybrid grouper when they were cultured in a similar system, p < .05. Meanwhile, there was no significant difference in intensity between the two groupers, p > .05. Within the hybrid groupers, the highest prevalence was obtained from hybrid groupers reared in the earthen ponds. Histopathological studies showed that the infected groupers exhibited inflammation, congestion and erosion of the epidermis layer. Hybrid grouper had more severe histopathological lesions in the skin tissues. These results suggested that species and type of aquaculture system had significantly determined the prevalence, intensity and severity of lesion in Z. arugamensis infestation.  相似文献   
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Toxoplasma gondii consists of three genotypes, namely genotype I, II and III. Based on its virulence, T. gondii can be divided into virulent and avirulent strains. This study intends to evaluate an alternative method for predicting T. gondii virulence using hierarchical cluster analysis based on complete coding sequences (CDS) of sag1, gra7 and rop18 genes. Dendrogram was constructed using UPGMA with a Kimura 80 nucleotide distance measurement. The results showed that the prediction errors of T. gondii virulence using sag1, gra7 and rop18 were 7.41%, 6.89% and 9.1%, respectively. Analysis based on CDS of gra7 and rop18 was able to differentiate avirulent strains into genotypes II and III, whereas sag1 failed to differentiate.  相似文献   
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ABSTRACT

The quality of finished wood products depends on the quality of its coating layer. An important indicator for evaluating the quality is Adherence of the paint on the wood surface. The purpose of this research was to analyze the effect of wood surface roughness and the viscosity of acrylic paints (pure acrylic and acrylic copolymer) to the wettability and Adherence for long and short rotation teaks. Wood surface with various degrees of roughness was prepared by sanding with abrasive papers of P120, P240, and P360 grits. Different viscosity of the acrylic paints was prepared composition between paint and water (w/w) of 100:0, 90:10, 80:20, and 70:30. The wettability of the acrylic paints on teak wood surfaces was measured using a sessile drop contact angle method with the S/G model. Adherence of the coating layer was measured using a crosscut test. The results showed that the increase in the roughness (Ra) and the decrease in the viscosity of the paints resulted in the increase in the wettability which leads to better Adherence of acrylic paints on the surface of teak woods. The pure acrylic paint generated better wettability and Adherence compared to the acrylic copolymer.  相似文献   
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On-farm trials were conducted to assess the effects of four branch pruning levels on maize grain yield, tree growth and stem shape. The experimental plots consisted of Gmelina (Gmelina arborea R.Br.) trees planted at 1 × 10 m with maize intercropped in the 10 m-wide alleys between lines of trees. Pruning levels consisted of retaining a live crown ratio of 60–70% (T 1), 40–50% (T 2); 30–40% (T 3) and of 20–30% (T 4). At the end of the experiment, the total maize grain yield was highest under the high pruning intensity (T 4) (18.06 t ha−1) and lowest under T 1 (14.48 t ha−1). Maize grain yield under the pruning regime T 2 and T 3 were 16.08 and 17.21 t ha−1, respectively. Mean annual increment (MAI) in tree diameter was greater (5.0 cm year−1) under T 1 than those at T 4 (4.1 cm year−1). Pruning regimes T 2 and T 3 resulted in a MAI of 4.7 and 4.5 cm year−1, respectively. Financial analysis showed that maize-tree systems under T 4 were more profitable than under T 1 as long as the reduction of the average dbh at harvest were not greater than 1 cm. Pruning trees intensively also generated greater returns from labour than moderate pruning, as the greater maize grain yields under T 4 compensated for the cost of pruning and the lower timber yield. In the context of resource-poor farmers, intensive branch pruning was a practice that prolonged the period of profitable intercropping and was compatible with commercial timber production.  相似文献   
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White sand heath forests (WS) or kerangas, an unusual variant of tropical forests in Borneo, characterized by open scrubby vegetation, low productivity, and distinctive plant species composition and soil microbial community, are regarded as a stressful low-pH and/or nutrient environment. We investigated whether the functional soil metagenome also shows a predicted set of indicators of stressful conditions in WS. Based on stress-tolerant strategies exhibited by larger organisms, we hypothesized that genes for stress tolerance, dormancy, sporulation, and nutrient processing are more abundant in the soil microbiota of WS. We also hypothesized that there is less evidence of biotic interaction in white sand soils, with lower connectivity and fewer genes related to organismic interactions. In Brunei, we sampled soils from a WS and a normal primary dipterocarp forest, together with an inland heath, an intermediate forest type. Soil DNA was extracted, and shotgun sequencing was performed using Illumina HiSeq platform, with classification by the Metagenomics Rapid Annotation using Subsystem Technology (MG-RAST). The results, on one hand, supported our hypothesis (on greater abundance of dormancy, virulence, and sporulation-related genes). However, some aspects of our results showed no significant difference (specifically in stress tolerance, antibiotic resistance, viruses, and clustered regularly interspaced short palindrome repeats (CRISPRs)). It appears that in certain respects, the extreme white sand environment produces the predicted strategy of less biotic interaction, but exhibits high soil microbiota connectivity and functional diversity.  相似文献   
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Parkia speciosa Hassk. (petai, pete, sator, and stink bean) is a medium to large tree native to Southeast Asia with various medicinal, wood, and shade tree uses. Its seed is an important component of Indonesian, Malaysian, and Thai cuisines. Petai is a common component of smallholder tree gardens throughout Indonesia. Indigenous propagation practices for this species are removal of the seed coat, cutting off ¼ to ½ of the seed, or both, before sowing in prepared beds or containers. These practices are thought by farmers to accelerate seed germination and improve seedling growth. Seed cutting is also practiced to retain part of the seed for household consumption. A research trial was implemented to document the effect of seed shelling and seed cutting on seed germination, seedling growth, and seedling survival. Results indicate that both treatments accelerate—but do not increase—seed germination. Nine days after sowing, the treatments increased germination by 27–32% over the controls; the combination of the treatments increased germination by up to 70%. However, by 35 days all treatments and combinations achieved 100% germination. Seed cutting had a negative effect on seedling diameter and height growth. The combination of no shelling and ½ cutting indicated higher seedling mortality over the study period. The findings support the use of the indigenous seed shelling practice but suggest discontinuation of seed cutting practices.  相似文献   
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