Correction to: Predation on glass eels of Japanese eel <Emphasis Type="Italic">Anguilla japonica</Emphasis> in the Tone River Estuary,Japan     

Yoichi Miyake  Aigo Takeshige  Hikaru Itakura  Hajime Itoh  Hiroaki Onda  Akira Yamaguchi  Akihito Yoneta  Kohma Arai  Yulina V. Hane  Shingo Kimura 《Fisheries Science》2018,84(6):1015-1016

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Alessandro Bonanno and Lawrence Busch (eds): Handbook of the international political economy of agriculture and food     

Marie Louise Ryan 《Agriculture and Human Values》2018,35(3):731-732

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萘乙酸对大白菜钙素吸收运转及防治干烧心病的研究   总被引：10，自引：1，他引：9  

闻凤英  孙德岭 《园艺学报》1991,18(2):148-152

本研究从补钙入手,探索防治干烧心病的方法。用0.7％CaCl_2·2H_2O对和50ppmNAA混合液在大白菜结球期叶面喷洒5次,经8年试验,对大白菜干烧心病的相对防治效果达80％以上。 在此基础上,采用~(45)Ca示踪试验的方法对防病机理做了进一步研究。结果表明,无论从根部还是从叶部加萘乙酸对根部和叶部~(45)Ca的吸收和运转均具有促进作用,并且改变了其在大白菜植株内的分配规律。对照株单位干叶重dpm值自外向内呈下降趋势(130×10~3─60×10~3);用NAA处理株则相反,自外向内呈增加趋势(120×10~3─250×10~3),说明萘乙酸具有促进钙素向叶球中内部运转的功效。这种效果在贮藏期间仍能保持。另外,~(45)Ca向直接受到NAA处理部位的运转量有明显增高的趋势。这一结果为补钙防治干烧心病提供了理论依据。  相似文献   

苹果果皮细胞膜结构变化与虎皮病的关系     

鞠志国  原永兵  刘成连  戴洪义  李志军 《果树学报》1992,(4)

国光苹果在贮藏过程中,果皮内α—法尼烯不断积累,60天左右达最大值,后随共轭三烯的迅速增加而下降。与此同时,果皮中丙二醛积累,组织相对电导率增加。抗氧化剂二苯胺能显著降低果皮中丙二醛积累,阻止相对电导率的上升,减少发病率。对膜结构变化与虎皮病的关系进行了讨论。  相似文献   

以CP23重组蛋白为抗原建立检测微小隐孢子虫抗体间接ELISA方法的研究   总被引：3，自引：0，他引：3  

何宏轩  张西臣  尹继刚  李建华  杨举 《中国兽医杂志》2003,39(1):3-5

摘要：以在E.coli高效表达的微小隐孢子虫子孢子表面抗原CP23为抗原，以辣根过氧化物酶(HRP)标记的山羊抗鼠IgG为二抗，建立了检测微小隐孢子虫抗体的间接ELISA方法。经检测筛选出最佳反应条件为1μg／孔纯化的E.coli表达的CP23抗原包被酶标板，用10％免血清进行封闭，以正常E.coli裂解上清液稀释待检血清。实验表明应用CP23重组蛋白作为诊断C.parvum抗原具有特异性高、抗原易纯化和成本低等特点。  相似文献   

胶合板厂生产规划模型与运用     

居志建  卢晓宁 《木材工业》1995,9(2):5-9

本文根据胶合板厂实际情况，建立了杨木胶合板厂生产规划模型。该模型考虑各主要设备工段、产品组合、产品混合、产品价格、流动资金、原材料供应以及市场约束等问题。本模型能够满足市场竞争，获取最佳利润，同时还能解决胶合板厂“三板”（表、背、芯单板）不平衡问题。  相似文献   

‘Plantibodies’: a flexible approach to design resistance against pathogens     

A. Schots  J. De Boer  A. Schouten  J. Roosien  J. F. Zil Verentant  H. Pomp  L. Bouwman-Smits  H. Overmars  F. J. Gommers  B. Visser  W. J. Stiekema  J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.  相似文献   

Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats          下载免费PDF全文

Sui Zhi Tian  Chai Ju Chang  Chih Chi Chiang  Huo Cheng Peh  Mu Chiou Huang  Jai-Wei Lee    Xin Zhao 《Canadian journal of veterinary research》2005,69(1):39-45

The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis.  相似文献   

‘三棱榄''橄榄果实香气成分分析   总被引：7，自引：1，他引：7  

钟明  陈玉芬  甘廉生  蔡时可 《园艺学报》2003,30(6):757-761

1 材料与方法选取广东优良鲜食橄榄品种‘三棱榄’,2001年12月6日采样,采用固相微萃取法(SPME)富集香气成分(鲜橄榄果肉于15℃下捣碎后取样1.0 g放入4 mL聚四氟乙烯硅橡胶垫密封螺口玻璃瓶中,插入100μm聚二甲基硅氧烷纤维头于室温25-30℃顶空取样2 h),用美国Finnigan TRACE GC-MS气相色谱-质谱联用仪进行分析。气相色谱柱为DB-1弹性毛细管柱30 m×0.25 mm,载气为He(99.99％),流速1.0mL/min。程序升温从40℃开始先保持10 min,后以2℃/min的升温速率升至150℃保持10 min。质谱条件:电子能量70 eV,离子源温度250℃,质量范围35-450 aum,不分流进样。2002年12月18日采样重复分析。  相似文献   

Characteristics for Practical Use of Attenuated Isolate UA-Fukushima of <Emphasis Type="Italic">Tomato mosaic virus</Emphasis>     

Tsutomu MATSUMOTO  Yuichiro NARA  Hiromitsu FURUYA  Harumi TAKAHASHI  Kiichi TAIRAKO  Hideki YAMAMOTO 《Journal of General Plant Pathology》2002,68(4):382-384

L₁₁A-Fukushima (L₁₁A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited. Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L₁₁A-F and L₁₁A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these results, L₁₁A-F appears to possess the properties necessary for practical use. To manage L₁₁A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L₁₁A-F preparations was established. Received 10 June 2002/ Accepted in revised form 30 October 2002  相似文献