Narrow genetic base in forest restoration with holm oak (Quercus ilex L.) in Sicily     

Concetta Burgarella  Miguel Navascués  Álvaro Soto  Ángel Lora  Silvio Fici 《Annals of Forest Science》2007,64(7):757-763

In order to empirically assess the effect of actual seed sampling strategy on genetic diversity of holm oak (Quercus ilex) forestations in Sicily, we have analysed the genetic composition of two seedling lots (nursery stock and plantation) and their known natural seed origin stand by means of six nuclear microsatellite loci. Significant reduction in genetic diversity and significant difference in genetic composition of the seedling lots compared to the seed origin stand were detected. The female and the total effective number of parents were quantified by means of maternity assignment of seedlings and temporal changes in allele frequencies. Extremely low effective maternity numbers were estimated (Nf _e ≈ 2–4) and estimates accounting for both seed and pollen donors gave also low values (N _e ≈ 35–50). These values can be explained by an inappropriate forestry seed harvest strategy limited to a small number of spatially close trees.  相似文献   

Extraction and characterization of Foeniculum vulgare pectins and their use for preparing biopolymer films in the presence of phaseolin protein     

Giosafatto CV  Mariniello L  Ring S 《Journal of agricultural and food chemistry》2007,55(4):1237-1240

Pectins from Foeniculum vulgare were extracted under acidic conditions. The obtained pectins were mainly composed of uronic acid but also contained traces of rhamnose, galactose, and arabinose. Extracted pectins were used as a carbohydrate source to prepare biopolymer films in the absence and in the presence of phaseolin protein. The swelling characteristics of the films were examined as a function of ionic strength, pH, and the applied osmotic stress. The swelling behavior was dominated by a Donnan-type effect, which decreases with increasing ionic strength and counterion valency. In all cases the swelling of films containing phaseolin was reduced, suggesting a network formation between protein and pectins. Mechanical property studies have also estimated the validity of the obtained novel biopolymer films in terms of mechanical resistance.  相似文献   

Synthesis and resistance to in vitro proteolysis of transglutaminase cross-linked phaseolin, the major storage protein from Phaseolus vulgaris     

Mariniello L  Giosafatto CV  Di Pierro P  Sorrentino A  Porta R 《Journal of agricultural and food chemistry》2007,55(12):4717-4721

The ability of phaseolin to act as an acyl donor and acceptor substrate of transglutaminase was studied by using an enzyme isolated from Streptoverticillium mobarense. Phaseolin, a trimeric storage protein from Phaseolus vulgaris L., was shown to possess both glutamine and lysine residues reactive for the enzyme. The extent of transglutaminase-catalyzed cross-linking has been studied in function of both incubation time and enzyme concentration. Native- and SDS-PAGE demonstrated that phaseolin is intra- and intermolecularly cross-linked by transglutaminase and gives rise to different polymers as well as to modified forms of the protein having a similar molecular weight but lower Stokes radius if compared to unmodified phaseolin. Cross-linked phaseolin was found to be more resistant to proteolytic cleavage than the unmodified counterpart, as demonstrated by in vitro trypsin and pepsin digestion experiments. This behavior could suggest novel possible uses of the transglutaminase-modified phaseolin.  相似文献   

Iceberg discharges into the north atlantic on millennial time scales during the last glaciation   总被引：2，自引：0，他引：2  

Bond GC  Lotti R 《Science (New York, N.Y.)》1995,267(5200):1005-1010

High-resolution studies of North Atlantic deep sea cores demonstrate that prominent increases in iceberg calving recurred at intervals of 2000 to 3000 years, much more frequently than the 7000-to 10,000-year pacing of massive ice discharges associated with Heinrich events. The calving cycles correlate with warm-cold oscillations, called Dansgaard-Oeschger events, in Greenland ice cores. Each cycle records synchronous discharges of ice from different sources, and the cycles are decoupled from sea-surface temperatures. These findings point to a mechanism operating within the atmosphere that caused rapid oscillations in air temperatures above Greenland and in calving from more than one ice sheet.  相似文献   

Uncommonly thorough hydrolysis of peptides during ripening of Ragusano cheese revealed by tandem mass spectrometry     

Gagnaire V  Carpino S  Pediliggieri C  Jardin J  Lortal S  Licitra G 《Journal of agricultural and food chemistry》2011,59(23):12443-12452

Ragusano is a pasta filata cheese produced from raw milk in Sicily. The proteolysis was extensively analyzed after stretching (day 0), at 4 and 7 months of ripening through soluble nitrogen, urea-PAGE, and peptide identification by tandem mass spectrometry. After stretching, 123 peptides were identified: 72 arising from β-casein, 34 from α(s1)-casein, and 17 from α(s2)-casein. The main protein splitting corresponded to the action of plasmin, chymosin, cathepsin D, cell envelope proteinase, and peptidase activities of lactic acid bacteria. Unlike other types of cheeses, <10% residual β- and α(s)-caseins remained intact at 7 months, indicating original network organization based on large casein fragments. The number of identified soluble peptides also dramatically decreased after 4 and 7 months of ripening, to 47 and 25, respectively. Among them, bioactive peptides were found, that is, mineral carrier, antihypertensive, and immunomodulating peptides and phosphopeptides.  相似文献   

Meroterpenes from Marine Invertebrates: Structures,Occurrence, and Ecological Implications     

Marialuisa Menna  Concetta Imperatore  Filomena D’Aniello  Anna Aiello 《Marine drugs》2013,11(5):1602-1643

Meroterpenes are widely distributed among marine organisms; they are particularly abundant within brown algae, but other important sources include microorganisms and invertebrates. In the present review the structures and bioactivities of meroterpenes from marine invertebrates, mainly sponges and tunicates, are summarized. More than 300 molecules, often complex and with unique skeletons originating from intra- and inter-molecular cyclizations, and/or rearrangements, are illustrated. The reported syntheses are mentioned. The issue of a potential microbial link to their biosynthesis is also shortly outlined.  相似文献   

NECROTIZING ENCEPHALITIS IN A YORKSHIRE TERRIER: CLINICAL, IMAGING, AND PATHOLOGIC FINDINGS     

Donatella  Lotti  DVM  PhD  Maria Tresa  Capucchio  DVM  Elena  Gaidolfi  DM  Monica  Merlo  DVM 《Veterinary radiology & ultrasound》1999,40(6):622-626

A 5-year-old, male Yorkshire Terrier had chronic and progressive neurologic signs compatible with lesions in the right brain stem and right forebrain. In magnetic resonance images of the brain there were multifocal lesions at different stages of evolution, consistent with an inflammatory disease. The lesions were located in the white matter of the cerebrum, in the diencephalon and mesencephalon. These lesions were hypointense in T1-weighted images and hyperintense in T2-weighted images with no mass effect and minimal enhancement with gadolinium. Necrotizing encephalitis was confirmed post mortem.  相似文献   

Antioxidant and Carbohydrate-Hydrolysing Enzymes Potential of Sechium edule (Jacq.) Swartz (Cucurbitaceae) Peel,Leaves and Pulp Fresh and Processed     

Monica Rosa Loizzo  Marco Bonesi  Francesco Menichini  Maria Concetta Tenuta  Mariarosaria Leporini  Rosa Tundis 《Plant foods for human nutrition (Dordrecht, Netherlands)》2016,71(4):381-387

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Weak effects of habitat type on susceptibility to invasive freshwater species: an Italian case study          下载免费PDF全文

Angela Boggero  Alberto Basset  Martina Austoni  Enrico Barbone  Luca Bartolozzi  Isabella Bertani  Alessandro Campanaro  Antonella Cattaneo  Fabio Cianferoni  Giuseppe Corriero  Ambrosius Martin Dörr  A. Concetta Elia  Gentile Francesco Ficetola  Lyudmila Kamburska  Gianandrea La Porta  Sara Lauceri  Alessandro Ludovisi  Elda Gaino  Enzo Goretti  Massimo Lorenzoni  Marina Manca  Aldo Marchetto  Giuseppe Morabito  Francesco Nonnis Marzano  Alessandro Oggioni  Cataldo Pierri  Nicoletta Riccardi  Giampaolo Rossetti  Nicola Ungaro  Pietro Volta  Silvia Zaupa  Diego Fontaneto 《水产资源保护:海洋与淡水生态系统》2014,24(6):841-852

Speciation of phytate ion in aqueous solution. Sequestering ability toward mercury(II) cation in NaClaq at different ionic strengths     

De Stefano C  Milea D  Porcino N  Sammartano S 《Journal of agricultural and food chemistry》2006,54(4):1459-1466

As a contribution to understanding the speciation of mercury in the environment and to the study of the sequestering ability of phytate (Phy) toward heavy metal and organometal cations, this paper describes the results of an investigation (at t = 25 degrees C by potentiometry, ISE-H+ glass electrode) of its interactions with mercury(II) cation in NaCl aqueous solutions at different ionic strengths (I = 0.15 and 1.0 mol L(-1)), in the pH range 2.5 < or = pH < or = 9.5 and considering metal-to-ligand ratios of 1:1 < or = Hg/Phy < or = 4:1. The formation of 11 HgiHjPhy(12-2i-j)(- species with i = 1 and 0 < or = j < or = 7 and i = 2 and 0 < or = j < or = 2 was observed. Their complex formation constant values proved to be fairly dependent on ionic strength. The speciation of phytic acid and mercury(II) is also dependent on the metal-to-ligand ratio; the dependence of the stability of phytate-mercury(II) species on the phytate protonation step was modeled, and an empirical predictive relationship was proposed. From the results obtained, phytate has very good sequestering ability toward Hg2+, even in the presence of considerable excesses of chloride ion, that is, another ligand strongly interacting with mercury; this supports future studies both on the use of plants that naturally synthesize it for phytoremediation purposes and on its direct application in remediation techniques.  相似文献