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García ME Caballero J Toni P Garcia I Martinez de Merlo E Rollan E Gonzalez M Blanco JL 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2000,47(4):243-249
We describe a case of canine mycoses initially diagnosed by clinical signs and enzyme-linked immunosorbent assay anti-fungal test, and later confirmed by the isolation of Paecilomyces sp. during the post-mortem examination. The fungus was isolated from lesions in the kidneys, mitral valve, abdominal aorta and vertebral discs. In this kind of process, it is important to identify the responsible agent early in order to make a study of anti-fungal susceptibility and establish effective treatment. 相似文献
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Guillermo Riesco Muñoz Óscar Santaclara Estévez Juan Gabriel Álvarez González Esther Merlo Sánchez 《European Journal of Forest Research》2014,133(4):623-630
The aim of this study was to establish the sources of variation in maritime pine wood properties related to appearance and strength, because of the importance of this wood in the sawing industry in Galicia (NW Spain). Ten trees from each of ten plots of the species (i.e., 100 trees) growing in Galicia were felled and sawn to produce the structural planks required for studying the presence of external strength-reducing characteristics. The planks were sampled from the basal logs to 16 m height in the stems, yielding cross sections of target dimensions 100 × 40 mm to 200 × 200 mm. We propose a model in which individual tree height and dominant height are used to predict the knottiness of planks from trees in mature stands. The abundance of externally visible knots on faces and edges of sawn planks is therefore more dependent on the stand location than on the prior silvicultural treatment of the stand. 相似文献
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Coffee agroecosystem performance under full sun, shade, conventional and organic management regimes in Central America 总被引：1，自引：0，他引：1
J. Haggar M. Barrios M. Bola?os M. Merlo P. Moraga R. Munguia A. Ponce S. Romero G. Soto C. Staver E. de M. F. Virginio 《Agroforestry Systems》2011,82(3):285-301
Changes in coffee economics are leading producers to reduce agrochemical use and increase the use of shade. Research is needed on how to balance the competition from shade trees with the provision of ecological services to the coffee. In 2000, long-term coffee experiments were established in Costa Rica and Nicaragua to compare coffee agroecosystem performance under full sun, legume and non-legume shade types, and intensive and moderate conventional and organic inputs. Coffee yield from intensive organic production was not significantly different from intensive conventional in Nicaragua, but in Costa Rica it was lower during three of the six harvests. Full sun coffee production over 6 years was greater than shaded coffee in Costa Rica (61.8 vs. 44.7 t ha?1, P = 0.0002). In Nicaragua, full sun coffee production over 5 years (32.1 t ha?1) was equal to coffee with shade that included Tabebuia rosea (Bertol.) DC., (27?C30 t ha?1) and both were more productive (P = 0.03) than coffee shaded with Inga laurina (Sw.) Willd. (21.6 t ha?1). Moderate input organic production was significantly lower than other managements under all shade types, except in the presence of Erythrina poepiggina (Walp.) O.F. Cook. Inga and Erythrina had greater basal area and nutrient recycling from prunings than other shade species. Intensive organic production increased soil pH and P, and had higher K compared to moderate conventional. Although legume shade trees potentially provide ecological services to associated coffee, this depends on management of the competition from those same trees. 相似文献
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This study was designed to evaluate whether the outcome of artificial insemination (AI) was affected by the metabolic and oxidative status of dairy cows. Seventy-nine inseminations in 40 cows, were classified, on the basis of blood progesterone (P4) and pregnancy-associated glycoprotein (PAG) concentrations and clinical confirmation of pregnancy into, three categories: (1) positive (AI+, resulted in pregnancy, n=26; 33%), (2) negative (AI-, did not result in pregnancy, n=49; 62%), and (3) embryonic mortality (EM, n=4; 5%). Reactive oxygen metabolites, biological antioxidant potential, oxidative stress index, body condition score, glucose, total proteins, albumin, urea, non-esterified fatty acids (NEFAs), cholesterol, triglycerides, haptoglobin and advanced oxidative protein products (AOPPs) were measured on the day of AI (day 0), and 30 and 42days later. Cows with EM had lower BCS scores (2.5) than AI+ (2.8) and AI- (2.9) cows (P<0.05). During the post-partum period, body condition score (BCS) increased and NEFAs decreased (P<0.05) suggesting a recovery from the negative energy balance (NEB). The only significant differences found were that the mean concentration of AOPPs was higher and that of albumin lower in EM cows than in AI+ and AI- (P<0.05) animals. Plasma concentration of reactive oxygen metabolites and biological antioxidant potential were not related to AI outcome. Further studies are required to confirm this finding and to clarify the role of oxidative status on cows' fertility. 相似文献
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Beta‐mercaptoethanol supplementation of in vitro maturation medium does not influence nuclear and cytoplasmic maturation of equine oocytes 
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下载免费PDF全文 B Merlo E Iacono D Bucci M Spinaci G Galeati G Mari 《Reproduction in domestic animals》2016,51(6):992-996
In vitro embryo production in the horse is still not as efficient as in other species. Oxidative stress negatively affects oocyte and embryo culture. To attenuate/minimize the oxidative stress, antioxidants such as low‐molecular thiol compounds can be added to culture media. Beta‐mercaptoethanol (BME) has been shown to improve maturation and embryo development in different species. The aim of this study was to investigate whether the addition to maturation medium of BME at common (0.1 mM) and high (0.7 mM) concentration could improve oocyte maturation also in the horse. Equine oocytes recovered from slaughterhouse ovaries were used. Meiotic configuration after in vitro maturation (IVM) and early embryo production after intracytoplasmic sperm injection (ICSI) were considered as criteria for assessing nuclear and cytoplasmic maturation, respectively. A total of 1,076 oocytes were analysed over two experiments: 848 (control n = 293, BME 0.1 n = 270, BME 0.7 n = 285) were stained with Hoechst 33342 and examined for nuclear stage after 26 hr of IVM, and 228 MII oocytes were fertilized by ICSI (control n = 83, BME 0.1 n = 65, BME 0.7 n = 80). Cleavage rates were determined after 60 hr of culture. Unlike results obtained in other species, the addition of BME did not influence maturation rates (51.9% control vs 55.6% BME 0.1 mM and 55.1% BME 0.7 mM), nor cleavage rates after ICSI (38.6% vs 38.5% and 41.3%, respectively). In conclusion, the addition of BME at 0.1 and 0.7 mM to the maturation medium, in our culture conditions, has no effect on nuclear and cytoplasmic maturation of equine oocytes. 相似文献
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Manuel Alejandro Merlo Ismael Cross Ana Rodríguez‐Rúa Manuel Manchado Laureana Rebordinos 《Aquaculture Research》2013,44(6):974-984
The meagre (Argyrosomus regius) is a commercially important species for fisheries, and aquaculture production has been increasing in recent years. However, this growing importance has not been matched by increased genetic knowledge of the species. For this reason, an initial approach has been made to understanding the genetics of the meagre, using three particular multigene families (45S and 5S ribosomal DNA and U2 snRNA gene) as both molecular and cytogenetic markers. Only one type of 5S rDNA and only one ITS‐1 (from 45S rDNA) were obtained, and these therefore could provide the basis for the development of genetic markers for this species. However, the U2 snRNA gene produced a multi‐band electrophoretic pattern, and some of these bands were demonstrated to be linked with the U1 snRNA gene. This linkage could also provide a good species‐specific marker which could be useful for tracing the evolutionary history of the Sciaenidae family. In this study, we have described for the first time the karyotype of the meagre: this is composed of 48 acrocentric chromosomes. Each of the three gene families were localized on different chromosome pairs, although the U2 snRNA gene probe was also located scattered throughout the genome of the meagre, as expected by the amplification pattern. 相似文献
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G Mari G Rizzato B Merlo E Iacono D Bucci E Seren C Tamanini G Galeati M Spinaci 《Reproduction in domestic animals》2010,45(2):331-335
Little information is available on the quality of stallion spermatozoa after sex sorting. The objectives of the present study were to assess the quality of sex‐sorted stallion spermatozoa and determine its fertilizing ability after hysteroscopic low dose insemination. Ejaculates from four stallions were collected and sorted by a MoFlo SX® flow cytometer/sperm sorter. Before and after sorting, spermatozoa were evaluated for motility by Computer Assisted Sperm Analysis, viability (SYBR 14‐propidium iodide), mitochondrial function (JC‐1) and acrosomal status (fluorescein isothiocyanate Pisum sativum agglutinin conjugated). A fertility trial was carried out on four mares (seven oestrous cycles) by hysteroscopic insemination, depositing 5 × 106 X‐bearing spermatozoa. Sex sorting resulted in a significant decrease (p < 0.001) in all motility characteristics. Sperm viability and percentage of spermatozoa with functional mitochondria were not affected by the sorting process, while the percentage of reacted spermatozoa was higher (p < 0.01) for non‐sorted than sorted spermatozoa. Pregnancy rate was 28.6% (2/7) after low dose hysteroscopic insemination. Only one pregnancy was carried to term with the birth of a healthy filly. In conclusion, despite the reduction in sperm motility, sex sorting did not impair stallion sperm viability and mitochondrial activity immediately post‐thaw; moreover, the sexed spermatozoa retained the ability to fertilize in vivo. 相似文献
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Donatella Lotti DVM PhD Maria Tresa Capucchio DVM Elena Gaidolfi DM Monica Merlo DVM 《Veterinary radiology & ultrasound》1999,40(6):622-626
A 5-year-old, male Yorkshire Terrier had chronic and progressive neurologic signs compatible with lesions in the right brain stem and right forebrain. In magnetic resonance images of the brain there were multifocal lesions at different stages of evolution, consistent with an inflammatory disease. The lesions were located in the white matter of the cerebrum, in the diencephalon and mesencephalon. These lesions were hypointense in T1-weighted images and hyperintense in T2-weighted images with no mass effect and minimal enhancement with gadolinium. Necrotizing encephalitis was confirmed post mortem. 相似文献