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排序方式: 共有115条查询结果,搜索用时 281 毫秒
1.
H Amasaki S Matsumoto M Takanosu M Daigo 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(6):1031-1036
The present study describes histological alterations and immunohistochemical distributions of extracellular matrices (ECMs) and the carbonic anhydrase isozyme-III (CA-III) during the period of bovine palatine ridge formation. Morphogenesis of bovine palatine ridges was preceded by epidermal placodes and the mesenchymal condensation (MC). During the early stages of less than 44 cm crown rump length (CRL), fibronectin (FN) was distributed densely in the MC. Strong reactions against type I collagen (C-1) were detected outer to the FN positive site. In the stages of more than 44 cm CRL, FN and C-1 were distributed diffusely in subepithelial mesenchyme. Laminin (LN) and type IV collagen were distributed in the epithelial and endothelial basement membranes (BMs) in all of the stages examined, except in the stage of 7 cm CRL, where LN was not detected only in the BM just beneath the epidermal placode. CA-III was detected in basal epithelial cells except for palatine ridge rudiments in the stages of more than 21 cm CRL. It is suggested that the expressions of LN and CA-III might play a role in the spatial determination of rudiments of bovine fetal palatine ridges. 相似文献
2.
Inducible responses in plants against pathogen attack play a major role in resistance to disease. The defense responses are
mostly associated with the expression of various kinds of inducible genes. We employed differential hybridization to isolate
elicitor-inducible genes (EIGs) of tobacco (Nicotiana tabacum cv. Samsun NN) using the tobacco-fungal elicitor system. A cDNA library was constructed from tobacco leaves treated for 12
hr with hyphal wall components (HWC) prepared from Phytophthora infestans, and six EIGs were identified. Expression of all EIGs was induced after inoculation with the soybean pathogen Pseudomonas syringae pv. glycinea (nonpathogenic on tobacco) or treatment with salicylic acid, and a variety of expression patterns of EIG mRNAs was observed.
Sequence analysis of EIG cDNAs revealed similarities to genes for SAR8.2 (EIG-B39 and EIG-D14), glycine-rich protein (EIG-G7), extensin (EIG-I30), acyltransferase (EIG-I24) and unknown protein (EIG-J7). Possible roles of EIG products in disease resistance are discussed.
Received 30 August 2000/ Accepted in revised form 30 November 2000 相似文献
3.
To investigate the action of dinotefuran (MTI-446, 1-methyl-2-nitro-3-(tetrahydro-3-furylmethyl)guanidine), a recently developed insecticide, on insect nicotinic acetylcholine receptors (nAChRs), we determined the potencies of the compound and 15 analogues in inhibiting the specific binding of [3H]epibatidine (EPI), a nAChR agonist, and [3H]alpha-bungarotoxin (alpha-BGT), a competitive nAChR antagonist, to the nerve cord membranes of American cockroaches (Periplaneta americana). Racemic dinotefuran inhibited [3H]EPI binding with an IC50 of 890 nM and [3H]alpha-BGT binding with an IC50 of 36.1 microM. Scatchard analysis indicated that the dinotefuran inhibition of [3H]EPI binding was a competitive one. Slight structural modification caused a drastic reduction in potency; only four analogues were found to be equipotent to or more potent than dinotefuran. Chloropyridinyl and chlorothiazolyl neonicotinoid insecticides displayed two or three orders of magnitude higher potency than dinotefuran. There was a good correlation between the IC50 values of tested compounds obtained with [3H]EPI and those obtained with [3H]alpha-BGT. A better correlation was observed between 3-h knockdown activities (KD50) against German cockroaches (Blattella germanica) and IC50 values obtained from [3H]EPI assays than between 24-h lethal activities (LD50) and IC50 values. While the results indicate that dinotefuran and its analogues interact with the ACh-binding site in cockroach nAChRs, it remains to be elucidated why they displayed lower potencies than those expected based on their insecticidal activities. 相似文献
4.
Masahiro MIYABE Azusa GIN Eri ONOZAWA Mana DAIMON Hana YAMADA Hitomi ODA Akihiro MORI Yutaka MOMOTA Daigo AZAKAMI Ichiro YAMAMOTO Mariko MOCHIZUKI Toshinori SAKO Katsutoshi TAMURA Katsumi ISHIOKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1201-1206
G protein-coupled receptor (GPR) 120 is an unsaturated fatty acid receptor, which is
associated with various physiological functions. It is reported that the genetic variant
of GPR120, p.Arg270His, is detected more in obese people, and this genetic variation
functionally relates to obesity in humans. Obesity is a common nutritional disorder also
in dogs, but the genetic factors have not ever been identified in dogs. In this study, we
investigated the molecular structure of canine GPR120 and searched for candidate genetic
variants which may relate to obesity in dogs. Canine GPR120 was highly homologous to those
of other species, and seven transmembrane domains and two N-glycosylation sites were
conserved. GPR120 mRNA was expressed in lung, jejunum, ileum, colon, hypothalamus,
hippocampus, spinal cord, bone marrow, dermis and white adipose tissues in dogs, as those
in mice and humans. Genetic variants of GPR120 were explored in client-owned 141 dogs,
resulting in that 5 synonymous and 4 non-synonymous variants were found. The variant
c.595C>A (p.Pro199Thr) was found in 40 dogs, and the gene frequency was significantly
higher in dogs with higher body condition scores, i.e. 0.320 in BCS4–5 dogs, 0.175 in BCS3
dogs and 0.000 in BCS2 dogs. We conclude that c.595C>A (p.Pro199Thr) is a candidate
variant relating to obesity, which may be helpful for nutritional management of dogs. 相似文献
5.
Mitigation of nitrous oxide (N2O) emission from swine wastewater treatment in an aerobic bioreactor packed with carbon fibers
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Takahiro Yamashita Ryoko Yamamoto‐Ikemoto Hiroshi Yokoyama Hirofumi Kawahara Akifumi Ogino Takashi Osada 《Animal Science Journal》2015,86(3):358-368
Mitigation of nitrous oxide (N2O) emission from swine wastewater treatment was demonstrated in an aerobic bioreactor packed with carbon fibers (CF reactor). The CF reactor had a demonstrated advantage in mitigating N2O emission and avoiding NOx (NO3 + NO2) accumulation. The N2O emission factor was 0.0003 g N2O‐N/gTN‐load in the CF bioreactor compared to 0.03 gN2O‐N/gTN‐load in an activated sludge reactor (AS reactor). N2O and CH4 emissions from the CF reactor were 42 g‐CO2 eq/m3/day, while those from the AS reactor were 725 g‐CO2 eq/m3/day. The dissolved inorganic nitrogen (DIN) in the CF reactor removed an average of 156 mg/L of the NH4‐N, and accumulated an average of 14 mg/L of the NO3‐N. In contrast, the DIN in the AS reactor removed an average 144 mg/L of the NH4‐N and accumulated an average 183 mg/L of the NO3‐N. NO2‐N was almost undetectable in both reactors. 相似文献
6.
Extraction and characterization of oil bodies from soy beans: a natural source of pre-emulsified soybean oil 总被引:1,自引:0,他引:1
Iwanaga D Gray DA Fisk ID Decker EA Weiss J McClements DJ 《Journal of agricultural and food chemistry》2007,55(21):8711-8716
Soybeans contain oil bodies that are coated by a layer of oleosin proteins. In nature, this protein coating protects the oil bodies from environmental stresses and may be utilized by food manufacturers for the same purpose. In this study, oil bodies were extracted from soybean using an aqueous extraction method that involved blending, dispersion (pH 8.6), filtration, and centrifugation steps. The influence of NaCl (0-250 mM), thermal processing (30-90 degrees C, 20 min) and pH (2-8) on the properties and stability of the oil bodies was analyzed using zeta-potential, particle size, and creaming stability measurements. The extracted oil bodies were relatively small ( d 32 approximately 250 nm), and their zeta-potential went from around +12 mV to -20 mV as the pH was increased from 2 to 8, with an isoelectric point around pH 4. The oil bodies were stable to aggregation and creaming at low (pH = 2) and high (pH >/= 6) pH values but were unstable at intermediate values (3 = pH = 5), which was attributed to their relatively low zeta-potential. The oil bodies were stable to aggregation and creaming at relatively low salt concentrations (NaCl = 25 mM, pH 7) but were unstable at higher values as a result of electrostatic screening effects. The oil bodies were stable to thermal processing from 30 to 90 degrees C (0 mM NaCl, pH 7), but there appeared to be a change in their interfacial properties (decrease in zeta-potential) at temperatures exceeding 60 degrees C. These results suggest that oil bodies extracted from soybeans have similar or improved stability compared to soybean oil emulsions produced from bulk ingredients and may provide a new way of creating functional soy products for the food industry. 相似文献
7.
Ito H Kobayashi E Li SH Hatano T Sugita D Kubo N Shimura S Itoh Y Tokuda H Nishino H Yoshida T 《Journal of agricultural and food chemistry》2002,50(8):2400-2403
In a search for possible antitumor agents from natural sources, megastigmane glycosides and polyphenolic constituents isolated from the leaves of Eriobotrya japonica (Rosaceae) were found to inhibit the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced activation of Epstein-Barr virus early antigen in Raji cells. Roseoside and procyanidin B-2 were among the active compounds found in an in vitro assay; these compounds were further assessed for antitumor activity in vivo in a two-stage carcinogenesis assay on mouse skin. Roseoside significantly delayed carcinogenesis induced by peroxynitrite (initiator) and TPA (promoter), and its potency was comparable to that of a green tea polyphenol, (-)-epigallocatechin 3-O-gallate, in the same assay. 相似文献
8.
I. Onishi A. Hongo T. Sasakuma T. Kawahara K. Kato H. Miura 《Genetic Resources and Crop Evolution》2006,53(5):985-992
Spelt wheat, Triticum spelta L., has been proved to be rich-sources of useful genes for tolerance to biotic and abiotic stress, and grain quality. But
this crop plant has some undesirable traits including glume tenacity and brittle rachis. Free-threshing and reduced fragility
of rachis are very important traits for cultivation. The objectives in the present study were to investigate genetic variation
of rachis fragility in a wide range of spelt accessions, to examine its genetic segregation pattern, and to clarify if rachis
fragility is associated with dosage of chromosome 5A in aneuploid lines of bread wheat. The results demonstrated that spelt
germplasm contains a wide range of rachis toughness, and thus selection of spelt wheat with desirable characteristics combined
with an appropriate level of tough rachis would be possible. Spike morphology in the F2 plants was segregated into the three types, square-headed, speltoid, and compactoid. The F2 plants with compactoid spikes had the most brittle rachis, followed by the speltoid and square-headed spike F2 plants. Rachis fragility in bread wheat also had genetic variation and was associated with dosage of chromosome 5A. 相似文献
9.
S Kamiya M Daigo 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(6):1179-1184
Gravid sclerosis of the uterine artery was histologically examined in multiparous cows. This sclerosis was characterized by a thickening of the intima and a lamination of the internal elastic lamina. It was clearly observed at non-pregnant and early-pregnant stages and in the late postpartum period, but it could not be detected at term-pregnant stages and in the early postpartum period. These results suggest that the gravid sclerosis disappears during pregnancy and re-establishes after parturition. The importance of local factors, e.g. hemodynamics, in the cyclic appearance of gravid sclerosis is discussed. 相似文献
10.