Site index estimation in Scots pine (Pinus sylvestris L.) stands in the High Ebro Basin (northern Spain) using soil attributes     

Bravo  F.; Montero  G. 《Forestry》2001,74(4):395-406

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Terrestrial-marine continuum of sedimentary natural organic matter in a mid-latitude estuarine system     

Bravo  Carlo  Millo  Christian  Covelli  Stefano  Contin  Marco  De Nobili  Maria 《Journal of Soils and Sediments》2020,20(2):1074-1086

Journal of Soils and Sediments - Humic acids (HA) have several environmental roles, but are particularly important in aquatic environments, being recognized as redox active natural organic matter...  相似文献   

Post-fire production of mushrooms in Pinus pinaster forests using classificatory models     

Pablo Vásquez Gassibe  Raul Fraile Fabero  María Hernández-Rodríguez  Juan Andrés Oria-de-Rueda  Felipe Bravo Oviedo  Pablo Martín-Pinto 《Journal of Forest Research》2014,19(3):348-356

This study was aimed at describing post-fire mushroom production in a Mediterranean ecosystem dominated by Pinus pinaster Ait. in the northwest of Spain and assessing the results by classificatory models. During the autumn periods of 2003–2006, fruit bodies from 115 fungal taxa were collected in burned and unburned areas and were further grouped into the following categories: saprotrophic/mycorrhizal; and edible/non-edible. After wildfires, a significant reduction in the number of fungal species and fruit body biomass production was observed. Based on this relevant information, the first simple classificatory model for this aim is provided. Nine alternative models based on classifications according to combinations of edibility and functional groups were fitted, and four fruiting body biomass production classes were defined as possible responses. As explanatory factors, time after fire and climatic variables significantly related to fruit body production were included. The best predictive results were obtained for edible and edible-mycorrhizal models, for which the correct classification rate of production classes was between 92 and 85 %. Moreover, the models obtained were applied to analyse the effect of time after fire on fungal production. Mycorrhizal and edible fungal production after fire was classified into the lowest class, whereas saprotrophic and non-edible species followed a contrary trend. The classificatory models can be useful to optimise management and harvest of these increasingly appreciated non-timber forest resources.  相似文献   

Carbon in heartwood,sapwood and bark along the stem profile in three Mediterranean <Emphasis Type="Italic">Pinus</Emphasis> species     

Celia Herrero de Aza  María Belén Turrión  Valentín Pando  Felipe Bravo 《Annals of Forest Science》2011,68(6):1067-1076

• Context   

Understanding biological processes in forests is necessary to orientate ecosystem management towards potential C sequestration. To achieve this, information is required about changes in forest biomass C pools, including the stem components (bark, sapwood and heartwood).  相似文献   

Comparative effects of food-derived polyphenols on the viability and apoptosis of a human hepatoma cell line (HepG2)     

Ramos S  Alía M  Bravo L  Goya L 《Journal of agricultural and food chemistry》2005,53(4):1271-1280

Consumption of fruits and vegetables, which are rich in polyphenols, has been associated with a reduced risk of chronic diseases such as cancer. Dietary polyphenols have antioxidant and antiproliferative properties that might explain their beneficial effect on cancer prevention. The aim of this study was to investigate the effects of different pure polyphenols [quercetin, chlorogenic acid, and (-)-epicatechin] and natural fruit extracts (strawberry and plum) on viability or apoptosis of human hepatoma HepG2 cells. The treatment of cells for 18 h with quercetin and fruit extracts reduced cell viability in a dose-dependent manner; however, chlorogenic acid and (-)-epicatechin had no prominent effects on the cell death rate. Similarly, quercetin and strawberry and plum extracts, rather than chlorogenic acid and (-)-epicatechin, induced apoptosis in HepG2 cells. Moreover, quercetin and fruit extracts arrested the G1 phase in the cell cycle progression prior to apoptosis. Quercetin and strawberry and plum extracts may induce apoptosis and contribute to a reduced cell viability in HepG2 cells.  相似文献   

In vitro determination of the indigestible fraction in foods: an alternative to dietary fiber analysis   总被引：3，自引：0，他引：3  

Saura-Calixto F  García-Alonso A  Goñi I  Bravo L 《Journal of agricultural and food chemistry》2000,48(8):3342-3347

Dietary fiber (DF) intakes in Western countries only accounts for about one-third of the substrates required for colonic bacterial cell turnover. There is a general trend among nutritionists to extend the DF concept to include all food constituents reaching the colon. In this line, a method to quantify the major nondigestible components in plant foods, namely, the indigestible fraction (IF), is presented. Analytical conditions for IF determination are close to physiological. Samples, analyzed as eaten, were successively incubated with pepsin and alpha-amylase; after centrifugation and dialysis, insoluble and soluble IFs were obtained. IF values include DF, resistant starch, resistant protein, and other associated compounds. IF contents determined in common foods (cereals, legumes, vegetables, and fruits) were higher than DF contents. Calculated IF intakes were close to the estimated amount of substrates reaching the colon. IF data could be more useful than DF data from a nutritional point of view; therefore, IF is proposed as an alternative to DF for food labeling and food composition tables.  相似文献   

Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay   总被引：20，自引：0，他引：20  

Pulido R  Bravo L  Saura-Calixto F 《Journal of agricultural and food chemistry》2000,48(8):3396-3402

Most nonenzymatic antioxidant activity (scavenging of free radicals, inhibition of lipid peroxidation, etc.) is mediated by redox reactions. The antioxidant (AO) activity of polyphenols (PPs), as ferric-reducing power, was determined for the first time using a modified FRAP (ferric reducing/antioxidant power) assay. Reaction was followed for 30 min, and both Fe(II) standards and samples were dissolved in the same solvent to allow comparison. Selected representative PPs included flavonoids (quercetin, rutin, and catechin), resveratrol, tannic acid, and phenolic acids (gallic, caffeic, and ferulic). Carotenoids (beta-carotene and zeaxanthine), ascorbic acid, Trolox, and BHA were included for comparison. Equivalent concentration 1 (EC(1)), as the concentration of AO with a reducing effect equivalent to 1 mmol/L Fe(II), was used to compare AO efficiency. PPs had lower EC(1) values, and therefore higher reducing power, than ascorbic acid and Trolox. Tannic acid and quercetin had the highest AO capacity followed by gallic and caffeic acids. Resveratrol showed the lowest reducing effect. Carotenoids had no ferric reducing ability. Polyphenol's AO efficiency seemed to depend on the extent of hydroxylation and conjugation.  相似文献   

Oxidative status during late pregnancy and early lactation in dairy cows   总被引：1，自引：0，他引：1  

Castillo C  Hernandez J  Bravo A  Lopez-Alonso M  Pereira V  Benedito JL 《Veterinary journal (London, England : 1997)》2005,169(2):286-292

In the last few years, the detection of free radical damage and the body's defences against it have become increasingly important in clinical medicine as a complementary tool in the evaluation of metabolic status. The aim of this study was to evaluate, under field conditions, the anti-oxidant status of healthy cows during late pregnancy and lactation onset using two parameters: (1) plasma levels of malondialdehyde (MDA), a degradation product of lipid peroxidation, and (2) total antioxidant status (TAS). Results were compared with those obtained in another group of cows with lesser metabolic demands. We also investigated possible relationships between antioxidant status markers and other relevant blood parameters. Our results confirmed the characteristic metabolic changes associated with late pregnancy and early lactation. MDA and TAS provided an accurate reflection of the internal physiological status of the animal. The data indicated increased lipid peroxidation around parturition, but with wide individual variations that may be attributable not only to the physiological stage but also to unknown factors that will have to be further considered in future studies.  相似文献   

Sperm membrane functionality in the dog assessed by flow cytometry     

Cheuquemán C  Bravo P  Treulén F  Giojalas L  Villegas J  Sánchez R  Risopatrón J 《Reproduction in domestic animals》2012,47(1):39-43

The objective assessment of sperm function increases the chances of predicting the fertilizing capacity of a fresh semen sample or diagnosing infertility problems. In this study, the available flow cytometry technique was used to determine the membrane functional capacity of canine spermatozoa. The second fractions of ejaculates from six dogs were pooled, and samples (n = 26) processed to determine the variables: sperm viability and plasma membrane integrity by Sybr-14/Pi staining; phosphatidylserine (PS) translocation by Annexin-V-FITC/PI labelling; acrosome membrane integrity by FITC-conjugated Pisum sativum agglutinin/PI labelling; and mitochondrial membrane potential (ΔΨm) by staining with JC-1. Means for the 26 examined samples indicated that 82.66 ± 2.8% of the viable spermatozoa showed an intact plasma membrane, 8.4 ± 2.6% were moribund, 72.7 ± 16% had an intact acrosome, 80.9 ± 17% had high ΔΨm and 8.1 ± 11% had PS translocation with a PS translocation index of 2.1 ± 3%. Motility was only correlated with PS translocation (R = 0.3901; p = 0.0488), and acrosome membrane integrity was correlated with PS translocation (R = -0.5816; p = 0.0018). This study provides objective physiological data on the functional capacity of canine spermatozoa.  相似文献   

Hypolipidemic effect in cholesterol-fed rats of a soluble fiber-rich product obtained from cocoa husks     

Ramos S  Moulay L  Granado-Serrano AB  Vilanova O  Muguerza B  Goya L  Bravo L 《Journal of agricultural and food chemistry》2008,56(16):6985-6993

A new soluble cocoa fiber product (SCFP), obtained after enzymatic treatment of cocoa husks, was characterized and its potential health effects studied in an animal model of dietary-induced hypercholesterolemia. The SCFP was rich in soluble dietary fiber (DF) and antioxidant polyphenols. Consumption of a cholesterol-rich diet containing the SCFP as a source of DF resulted in lower food intake and body weight gain in comparison with control groups consuming cholesterol-free or cholesterol-rich diets with cellulose as DF. The cholesterol-rich diet caused remarkable hypercholesterolemia. However, the SCFP diminished the negative impact of the cholesterol-rich diet, buffering the decrease of high density lipoprotein-cholesterol, and the increase of total and low density lipoprotein-cholesterol levels, and lipid peroxidation (malondialdehyde levels) induced by the fatty diet. The SCFP also decreased triglyceride levels to values lower than those in the group fed the cholesterol-free diet. These results put forward the potential application of the SCFP as a dietary supplement or functional food ingredient.  相似文献