首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   435篇
  免费   18篇
林业   16篇
农学   7篇
  47篇
综合类   70篇
农作物   10篇
水产渔业   12篇
畜牧兽医   262篇
园艺   12篇
植物保护   17篇
  2023年   6篇
  2022年   5篇
  2021年   4篇
  2020年   13篇
  2019年   13篇
  2018年   9篇
  2017年   5篇
  2016年   7篇
  2015年   10篇
  2014年   18篇
  2013年   14篇
  2012年   24篇
  2011年   19篇
  2010年   22篇
  2009年   8篇
  2008年   21篇
  2007年   17篇
  2006年   26篇
  2005年   27篇
  2004年   25篇
  2003年   22篇
  2002年   26篇
  2001年   5篇
  2000年   2篇
  1999年   6篇
  1998年   2篇
  1997年   2篇
  1996年   2篇
  1994年   2篇
  1993年   2篇
  1992年   4篇
  1991年   3篇
  1990年   4篇
  1986年   7篇
  1985年   7篇
  1984年   4篇
  1983年   4篇
  1982年   2篇
  1981年   5篇
  1980年   3篇
  1979年   2篇
  1972年   2篇
  1970年   3篇
  1967年   3篇
  1966年   3篇
  1910年   2篇
  1888年   1篇
  1885年   1篇
  1884年   8篇
  1883年   6篇
排序方式: 共有453条查询结果,搜索用时 234 毫秒
1.
2.
Plant resistance to insect herbivory involves gene expression in response to wounding and the detection of insect elicitors in oral secretions (Kessler and Baldwin, 2002, Ann. Rev. Plant/ Biol. 53: 299–328). However, crawling insect larvae stimulate the synthesis of 4-aminobutyrate within minutes and imprints of larval footsteps can be visualized within seconds through superoxide production or transient increases in chlorophyll fluorescence (Bown et al., 2002, Plant Physiol. 129: 1430–1434). Here cryo-scanning electron microscopy was used to demonstrate that larval feet, which are equipped with a perimeter row of hook-like crochets, damage leaf tissue and result in larval footprints. Staining for cell death shows that areas of wounding correspond to footsteps detected through increased chlorophyll fluorescence. Superoxide production in response to footsteps was inhibited by diphenyleneiodonium, an inhibitor of the plasma membrane NADPH oxidase enzyme. Inhibition of superoxide production, however, did not eliminate the detection of cell death. The results demonstrate that larval footsteps damage leaf tissue, and initiate rapid local responses which are not dependent on herbivory or oral secretions. It is proposed that superoxide production at the wound site prevents opportunistic pathogen infection.  相似文献   
3.
4.
5.
6.
Three hundred and eighty Salmonella isolates recovered from animal diagnostic samples obtained from four state veterinary diagnostic laboratories (AZ, NC, MO, and TN) between 2002 and 2003 were tested for antimicrobial susceptibilities and further characterized for bla(CMY) beta-lactamase genes, class 1 integrons and genetic relatedness using PFGE. Forty-seven serovars were identified, the most common being S. Typhimurium (26%), S. Heidelberg (9%), S, Dublin (8%), S. Newport (8%), S. Derby (7%), and S. Choleraesuis (7%). Three hundred and thirteen (82%) isolates were resistant to at least one antimicrobial, and 265 (70%) to three or more antimicrobials. Resistance was most often observed to tetracycline (78%), followed by streptomycin (73%), sulfamethoxazole (68%), and ampicillin (54%), and to a lesser extent chloramphenicol (37%), kanamycin (37%), amoxicillin-clavulanic acid (20%), and ceftiofur (17%). With regards to animal of origin, swine Salmonella isolates displayed the highest rate of resistance, being resistant to at least one antimicrobial (92%), followed by those recovered from turkey (91%), cattle (77%), chicken (68%), and equine (20%). Serovars commonly showing multidrug resistance (MDR) to > or =9 antimicrobials were S. Uganda (100%), S. Agona (79%), and S. Newport (62%), compared to S. Heidelberg (11%) and S. Typhimurium (7%). Class-1 integrons were detected in 43% of all isolates, and were found to contain aadA, aadB, dhfr, cmlA and sat1 gene cassettes alone or in various combinations. All ceftiofur resistant isolates (n=66) carried the bla(CMY) beta-lactamase gene. A total of 230 PFGE patterns were generated among the 380 isolates tested using XbaI, indicating extensive genetic diversity across recovered Salmonella serovars, however, several MDR clones were repeatedly recovered from different diseased animals.  相似文献   
7.
8.
A method for the separation of the oil, emulsifier and polymer or salt fractions of adjuvants is described. The adjuvant is dissolved in 90% aqueous ethanol and filtered to remove polymers or salt. Light petroleum (distillation range 30–40°C) is added to the solution in a separating funnel. The light petroleum fraction is subsequently extracted with successive aliquots of 80, 70, 60 and 50% aqueous ethanol. The whole of the ethanol and light petroleum fractions are evaporated to dryness to yield the emulsifier and oil fractions respectively. Emulsifier, oil and polymer or salt are determined gravimetrically. The method successfully detected the presence of polymer or salt in adjuvants, but could not distinguish between them when both were present in the same product. The adjuvants could be classified into four broad classes; those containing > 70% emulsifier (wetter-spreaders), those containing > 75% oil (anti-evaporants), those containing a high proportion of polymer (film-forming substances), and those containing comparable amounts of all three components (multi-purpose adjuvants).  相似文献   
9.
Fruit-set of ‘Golden Delicious’ apple trees was found to be largely independent of seasonal differences and much more closely related to the initial number of blossom clusters, when compared with many other apple cultivars under English conditions. In one young orchard, yield became increasingly a function of trunk girth during the first 3 cropping-years, but the trees then abruptly developed a biennial rhythm. Thereafter, blossom production was negatively correlated with the number of clusters in the previous year rather than with the number of fruits.Applications of daminozide or GA3 appeared to increase or decrease, respectively, the amount of return bloom without altering the negative correlation with the number of clusters in the previous year. Chemical fruit-thinning with a mixture of carbaryl and NAA also appeared to be effective in increasing return bloom.  相似文献   
10.
Beta2 toxin, encoded by the cpb2 gene, has been implicated in the pathogenesis of porcine, equine and bovine enteritis by type A Clostridium perfringens. By incorporating primers to cpb2 into a multiplex genotyping PCR, we screened 3270 field isolates of C. perfringens. Of these, 37.2% were PCR positive for the cpb2 gene. The majority of isolates from cases of porcine enteritis were positive for cpb2 (>85%), and this was even more true for C. perfringens isolated from cases of porcine neonatal enteritis (91.8%). In contrast, isolates from normal pigs only contained cpb2 in 11.1% of cases. The correlation between enteritis in other animal species and the presence of cpb2 was not so strong. cpb2 was found in 21.4% of C. perfringens isolates from cattle with enteritis, and in 47.3% of isolates from calves with enteritis or abomastitis. The prevalence of cpb2 varied with genotype, with type A isolates being positive for this gene in 35.1% of cases. Furthermore, enterotoxigenic type D or type E strains almost always carried cpb2. We cloned a 6xHIS-tagged beta2 (HIS-beta2) and used this protein to raise antiserum against beta2. Culture supernatants from 68 cpb2-positive and 13 cpb2-negative strains were tested for the presence of beta2 by Western blotting. In cpb2-positive isolates of porcine origin, beta2 was almost always detected (96.9%). However, in cpb2-positive isolates from other animal species, only 50.0% expressed beta2 protein. The high rate of cpb2-positivity among strains from neonatal pigs with enteritis and the high correlation of genotype with phenotype, supports the contention that beta2 toxin plays a role in the pathogenesis of these infections. However, it may be important to consider the use of an additional method for the detection of beta2 toxin in non-porcine cpb2-positive isolates when making claims about the role of beta2 in enteritis in non-porcine species.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号