Characterization of and radioimmunoassay for canine thyroglobulin     

C. P. Verschueren  P. J. Selman  J. J. M. de Vijlder  J. A. Mol 《Domestic animal endocrinology》1991,8(4):509-519

Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0 % (w/w). cTg did not cross-react with antibodies against human Tg to a degree that would allow the use of a radioimmunoassay for human Tg for the determination of cTg in serum or plasma. Therefore a polyclonal antiserum was raised against cTg and a homologous radioimmunoassay was developed, which was sensitive (0.4 μg/l) and specific (cross-reactivity in cTg assay of human Tg, goat Tg, T₄, T₃, and DIT < 0.01 %).

Plasma Tg levels in normal dogs of both sexes and aged 3–15 years amounted to 192 ± 73 μg/l (mean ± SD, n=30). There was no relation between plasma Tg and T₄ levels.  相似文献   

Effect of plant roots on the germination of microsclerotia ofVerticillium dahliae     

L. Mol 《European journal of plant pathology / European Foundation for Plant Pathology》1995,101(6):679-685

Induction of germination of microsclerotia by exudates from plant roots may be important for the control ofV. dahliae. Laboratory experiments with root observation boxes were carried out to assess the influence of root tips of seven crop species and cultivars on the germination of microsclerotia ofVerticillium dahliae in soil under controlled conditions. The root density of crops was measured in a field experiment. The results of the laboratory experiments and the field experiment were combined to estimate the total effect of crops on the population of microsclerotia in the field. Germination of microsclerotia was stimulated by all crops compared to a control without a crop. Among crops, roots of potato cvs Element and Astarte had a larger stimulation effect on microsclerotia than that of potato Ostara, pea, flax, sugar beet or onion. The number of hyphae per microsclerotium decreased with distance from the root surface regardless of the crop species or cultivar. Differences in root densities, in the affected root zones and in the stimulation effect on germination of microsclerotia caused large differences among crops in the effect on the population of microsclerotia in the soil. However, growing a rop with the special purpose to reduce the level ofV. dahliae inoculum in the soil is an inefficient control measure, because only a small part of the total soil volume is affected by roots and the number of hyphae per microscleroium affected is too low.Abbreviations MS microsclerotia, microsclerotium  相似文献   

Adenohypophyseal function in bitches treated with medroxyprogesterone acetate     

Beijerink NJ  Bhatti SF  Okkens AC  Dieleman SJ  Mol JA  Duchateau L  Van Ham LM  Kooistra HS 《Domestic animal endocrinology》2007,32(2):63-78

The aim of this study was to investigate the effects of treatment with medroxyprogesterone acetate (MPA) on canine adenohypophyseal function. Five Beagle bitches were treated with MPA (10mg/kg, every 4 weeks) and their adenohypophyseal function was assessed in a combined adenohypophyseal function test. Four hypophysiotropic hormones (CRH, GHRH, GnRH, and TRH) were administered before and 2, 5, 8, and 11 months after the start of MPA treatment, and blood samples for determination of the plasma concentrations of ACTH, cortisol, GH, IGF-1, LH, FSH, prolactin, alpha-MSH, and TSH were collected at -15, 0, 5, 10, 20, 30, and 45 min after suprapituitary stimulation. MPA successfully prevented the occurrence of estrus, ovulation, and a subsequent luteal phase. MPA treatment did not affect basal and GnRH-induced plasma LH concentrations. The basal plasma FSH concentration was significantly higher at 2 months after the start of MPA treatment than before or at 5, 8, and 11 months after the start of treatment. The maximal FSH increment and the AUC for FSH after suprapituitary stimulation were significantly higher before treatment than at 5, 8, and 11 months of MPA treatment. Differences in mean basal plasma GH concentrations before and during treatment were not significant, but MPA treatment resulted in significantly elevated basal plasma IGF-1 concentrations at 8 and 11 months. MPA treatment did not affect basal and stimulated plasma ACTH concentrations, with the exception of a decreased AUC for ACTH at 11 months. In contrast, the maximal cortisol increment and the AUC for cortisol after suprapituitary stimulation were significantly lower during MPA treatment than prior to treatment. MPA treatment did not affect basal plasma concentrations of prolactin, TSH, and alpha-MSH, with the exception of slightly increased basal plasma TSH concentrations at 8 months of treatment. MPA treatment did not affect TRH-induced plasma concentrations of prolactin and TSH. In conclusion, the effects of chronic MPA treatment on adenohypophyseal function included increased FSH secretion, unaffected LH secretion, activation of the mammary GH-induced IGF-I secretion, slightly activated TSH secretion, suppression of the hypothalamic-pituitary-adrenocortical axis, and unaffected secretion of prolactin and alpha-MSH.  相似文献   

Automated detection of estrus and mastitis in dairy cows     

de Mol RM 《Tijdschrift voor diergeneeskunde》2001,126(4):99-103

The development and test of detection models for oestrus and mastitis in dairy cows is described in a PhD thesis that was defended in Wageningen on June 5, 2000. These models were based on sensors for milk yield, milk temperature, electrical conductivity of milk, and cow activity and concentrate intake, and on combined processing of the sensor data. The models alert farmers to cows that need attention, because of possible oestrus or mastitis. A first detection model for cows, milked twice a day, was based on time series models for the sensor variables. A time series model describes the dependence between successive observations. The parameters of the time series models were fitted on-line for each cow after each milking by means of a Kalman filter, a mathematical method to estimate the state of a system on-line. The Kalman filter gives the best estimate of the current state of a system based on all preceding observations. This model was tested for 2 years on two experimental farms, and under field conditions on four farms over several years. A second detection model, for cow milked in an automatic milking system (AMS), was based on a generalization of the first model. Two data sets (one small, one large) were used for testing. The results for oestrus detection were good for both models. The results for mastitis detection were varying (in some cases good, in other cases moderate). Fuzzy logic was used to classify mastitis and oestrus alerts with both detection models, to reduce the number of false positive alerts. Fuzzy logic makes approximate reasoning possible, where statements can be partly true or false. Input for the fuzzy logic model were alerts from the detection models and additional information. The number of false positive alerts decreased considerably, while the number of detected cases remained at the same level. These models make automated detection possible in practice.  相似文献   

Effect of plant roots on the germination of microsclerotia ofVerticillum dahliae     

L. Mol  H. W. van Riessen 《European journal of plant pathology / European Foundation for Plant Pathology》1995,101(6):673-678

Root observation boxes were used to study the effects of hosts and non-hosts on the germination of microsclerotia ofV. dahliae. The effects of roots on microsclerotia were examined within a radius of 1 mm around the root tip. Host plants such as potato and field bean induced a higher percentage of germination of the microsclerotia than a non-host such as barley. A susceptible potato cultivar stimulated germination more than a resistant cultivar. The germination percentage and the number of hyphae per microsclerotium decreased with distance from the root surface regardless of the plant species or cultivar.  相似文献   

Integrated pest management of Tuta absoluta: practical implementations across different world regions     

Desneux  Nicolas  Han  Peng  Mansour  Ramzi  Arn&#;  Judit  Br&#;vault  Thierry  Campos  Mateus R.  Chailleux  Anais  Guedes  Raul N. C.  Karimi  Javad  Konan  Kouassi Arthur J.  Lavoir  Anne-violette  Luna  Mar&#;a G.  Perez-Hedo  Meritxell  Urbaneja  Alberto  Verheggen  Fran&#;ois J.  Zappal&#;  Lucia  Abbes  Khaled  Ali  Abid  Bayram  Yunus  Cantor  Fernando  Cuthbertson  Andrew G. S.  De Vis  Raf  Erler  Fedai  Firake  Dnyaneshwar M.  Haddi  Khalid  Hajjar  M. Jamal  Ismoilov  Khasan  Jaworski  Coline C.  Kenis  Marc  Liu  Hao-tian  Madadi  Hossein  Martin  Thibaud  Mazih  Ahmed  J. Messelink  Gerben  Mohamed  Samira A.  Nofemela  Robert S.  Oke  Abiola  Ramos  C&#;sar  Ricupero  Michele  Roditakis  Emmanouil  Shashank  Pathour R.  Wan  Fang-Hao  Wang  Ming-hui  Wang  Su  Zhang  Yi-Bo  Biondi  Antonio 《Journal of pest science》2022,95(1):17-39

Journal of Pest Science - The South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), has invaded most Afro-Eurasian countries and is threatening worldwide tomato...  相似文献   

Caudal Fossa Respiratory Epithelial Cyst in a Dog: Clinical,Magnetic Resonance Imaging,and Histopathologic Findings     

J. Molín  K. Rentmeister  K. Matiasek 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(4):1336-1340

  相似文献   

Lignin degradation by Agaricus bisporus accounts for a 30% increase in bioavailable holocellulose during cultivation on compost     

ten Have R  Wijngaard H  Ariës-Kronenburg NA  Straatsma G  Schaap PJ 《Journal of agricultural and food chemistry》2003,51(8):2242-2245

The common mushroom Agaricus bisporus is a non-white rot saphrophytic fungus that can degrade lignin to free and utilize holocellulose embedded in fermented straw as present in compost. A new method is described to estimate the actual amount of bioavailable holocellulose in 3.8 kg compost cultures spawned with A. bisporus Horst U1 prior to and during a cultivation with two cycles of mushroom harvesting. The method shows that the initial amount of bioavailable holocellulose per culture, accounting for 130 +/- 22 g, is lower than the total holocellulose consumption by A. bisporus accounting for 182 +/- 15 g. This difference is explained by a 30% increase in bioavailable holocellulose. The increase is caused by the degradation of 95 +/- 3 g of holocellulose-shielding lignin. The results are discussed within the scope of the A. bisporus mushroom yield and lignin degradation by white rot fungi during growth on lignocellulose-containing materials.  相似文献   

Udder oedema associated with adrenocortical insufficiency in a herd of Holstein/Friesian cows     

J H van der Kolk  T Wensing  H J Breukink  G H Wentink  J A Mol 《The Veterinary record》1991,128(7):149-152

The possible involvement of the pituitary-adrenocortical axis with severe pre- and post partum udder oedema was studied in a herd of 60 Holstein/Friesian cows. The prevalence of udder oedema in this herd was about 75 per cent. The animals were fed a diet containing high levels of potassium (average daily potassium intake 410 g per cow). The concentrations of sodium, potassium, chloride and total protein in the plasma of the cows were within their normal ranges. In one animal a very low plasma cortisol concentration (2.30 ng/ml) was accompanied by normal plasma glucose and beta-hydroxybutyrate concentrations. The adrenal responsiveness of two heifers with severe udder oedema was impaired one month after parturition in comparison with 11 normal animals (increases from 3.27 to 38.8 and 23.2 to 37.8 ng cortisol/ml compared with 8.02 +/- 1.19 to 146 +/- 16.7 ng cortisol/ml but returned to normal spontaneously after 175 days. One cow was diagnosed as having primary adrenocortical insufficiency.  相似文献   

Central Diabetes Insipidus in a Dog With a Pro-Opiomelanocortin-Producing Pituitary Tumor ot Causing Hyperadrenocorticism     

Mariëlle M.C. Goossens  Ad Rijnberk  Jan A. Mol  Jeannette Wolfswinkel  George Voorhout 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1995,9(5):361-365

Central diabetes insipidus was diagnosed by vasopressin measurements during hypertonic stimulation in a 9-year-old male giant Schnauzer with polyuria and polydipsia. The impaired release of vasopressin was believed to be caused by a large pituitary tumor, which was visualized by computed tomography. Studies of the function of the anterior lobe and the pars intermedia of the pituitary gland were conducted, and high concentrations of ACTH and α-melanotrophic hormone (α-MSH) were found without concomitant hyperadrenocorticism. Studies of the molecular size of the immunoreactive ACTH in plasma by gel filtration revealed that most of the circulating immunoreactivity was not ACTH but its precursor pro-opiomelanocortin (POMC) and low-molecular-weight POMC-derived peptides. The pituitary tumor of this dog probably originated from melanotrophic cells of the pars intermedia. The sensitivity of the pituitary-adrenocortical system for the suppressive effect of dexamethasone was unaffected.  相似文献