Manoalide derivatives from a marine sponge Luffariella sp. collected in Palau     

Michio  NAMIKOSHI  Shigeo  SUZUKI  Shiori  MEGURO  Hiroshi  NAGAI  Yoshio  KOIKE  Akira  KITAZAWA  Hisayoshi  KOBAYASHI  Taiko  ODA  Junko  YAMADA 《Fisheries Science》2004,70(1):152-158

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A note on the genetic diversity of the vulnerable aquatic macrophyte Sparganium erectum and its congeners (Sparganiaceae) in rural wetland in Japan     

TEMPEI ISHII  YUICHIRO NAKAYAMA  MIKIO KOBAYASHI  HIROFUMI YAMAGUCHI 《Weed Biology and Management》2004,4(4):230-234

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Chronic low-dose exposure to imidacloprid potentiates high fat diet-mediated liver steatosis in C57BL/6J male mice     

Collins NIMAKO  Yoshinori IKENAKA  Yuko OKAMATSU-OGURA  Jussiaea V. BARIUAN  Atsushi KOBAYASHI  Ryo YAMAZAKI  Kumiko TAIRA  Nobuhiko HOSHI  Tetsushi HIRANO  Shouta M. M. NAKAYAMA  Mayumi ISHIZUKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):487

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Impact of nutrient enrichment in a waterchestnut ecosystem at Takahama-iri Bay of Lake Kasumigaura,Japan     

Hidekazu Yamamoto  Humitake Seki 《Water, air, and soil pollution》1979,12(4):503-510

Waterchestnut was shown to be one of the most efficient agents to yield photosynthetic products among aquatic plants in littoral vegetation. Though the plant can be an agent to remove nutrient in agricultural and domestic wastes directly through its waterroots to some extent, waterchestnut plays a great role in removing nutrients through its roots from lake sediments to watercolumn of the lake. Waterchestnut can be choked to death when its leaves are covered completely by the heavy blue-green algal bloom typical in hypereutophic lakes.  相似文献   

Combination of lipids and emulsifiers enhances the absorption of orally administered quercetin in rats     

Azuma K  Ippoushi K  Ito H  Higashio H  Terao J 《Journal of agricultural and food chemistry》2002,50(6):1706-1712

The effects of lipids, emulsifiers, and ethanol on the absorption of orally administered quercetin in rats were investigated for its efficient intestinal absorption. Rats were administered 150 micromol/kg quercetin in water supplemented with lipids and/or emulsifiers, or ethanol, and blood was collected from the tail for 6 h after administration. Co-administration of lipids such as lecithin and soybean oil or emulsifiers including sucrose fatty acid ester, polyglycerol fatty acid ester, and sodium taurocholate had no statistically significant effects on quercetin absorption, although these constituents rather increased the accumulation of conjugated forms of quercetin and those of isorhamnetin in rat plasma. However, the combination of lipids and emulsifiers enhanced the absorption of quercetin significantly. Thirty and fifty percent (v/v) of the ethanol in the vehicle raised the efficiency of quercetin absorption in a concentration-dependent manner. Quercetin absorption-enhancing effects of these constituents seemed to be affected by quercetin's solubility in respective vehicles used for the administration. Ethanol is not helpful for the effective absorption of quercetin, as a high concentration is required. In conclusion, a combination of lipids and emulsifiers is necessary for enhancing quercetin absorption.  相似文献   

Effects of tris(1,3-dichloro-2-propyl) phosphate on epididymal sperm parameters in adult male rats     

Shohei KOBAYASHI  Natsuko KAWANO  Kenji MIYADO  Ryo OHTA  Takahiro AKIMOTO  Taichi HATAKEYAMA  Maiko KAWAGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):153

Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is widely used as a flame retardant and is known to exhibit anti-androgenic effects in vitro and in vivo. To assess the reproductive toxicity potency of TDCIPP, we investigated the effects of 7 days of TDCIPP oral administration on epididymal sperm motion and concentration in adult male Wistar–Imamichi rats. Thirty-five days after the final administration, sperm parameters were evaluated by computer-assisted sperm analysis. Results showed that sperm swimming progression and vigor and sperm concentration in TDCIPP-treated rats were unexpectedly higher than those in control rats. TDCIPP did not significantly affect the percentage of motile sperms or sperm swimming pattern. These results contribute to the understanding of the biological effects of TDCIPP.  相似文献   

Validation and application of real-time polymerase chain reaction assays for representative rumen bacteria     

Satoshi KOIKE  Hiroyoshi YABUKI  Yasuo KOBAYASHI 《Animal Science Journal》2007,78(2):135-141

Real‐time polymerase chain reaction (PCR) assays for 11 representative rumen bacterial species were validated. The sensitivity was tested by using the serially diluted target 16S rDNA from respective bacterial species. The recovery of the target DNA and the assay reproducibility were determined using DNA from rumen fluid spiked with different quantities of the target. Minimum detection levels for the target were 10–100 copies in pure culture. The recovery of the added target ranged from 82.4 to 116.6%. The intra‐ and inter‐assay variations of each assay were <9.4 and <12.6%, respectively. Therefore, the real‐time PCR assays evaluated in the present study are considered to be sufficiently reliable for monitoring all 11 bacterial species in the rumen. The assays were then applied to the monitoring of the bacterial species attached to ruminally incubated rice straw. Among the monitored fibrolytic species, Fibrobacter succinogenes was found to be the most dominant, accounting for 2.61% of total bacteria after 24 h incubation. Selenomonas ruminantium and Streptococcus bovis, non‐fibrolytics, were detected on the rice straw at 8.96% and 1.16% of total bacteria, respectively. Such high levels of non‐fibrolytics on the plant fiber suggest a synergistic relationship between fibrolytics and non‐fibrolytics.  相似文献   

Soybean sowing date effects on weed communities in untilled and tilled fields in north-eastern Japan     

HIROYUKI KOBAYASHI  ATSUSHI OYANAGI 《Weed Biology and Management》2006,6(3):177-181

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Adrenocorticotropic hormone-induced secretion of cortisol in goats is inhibited by androgen     

Yuko MAEJIMA  Masato AOYAMA  Noriko KOBAYASHI  Shoei SUGITA 《Animal Science Journal》2006,77(1):87-94

In a previous study, it was found that there are sex differences in goats with respect to the levels of cortisol secretion induced by transportation stress. We also found that treatment of castrated male goats with dihydrotestosterone (DHT) suppressed the increase in plasma cortisol concentration following transportation, but did not suppress the secretion of adrenocorticotropic hormone (ACTH). This suggests that androgen might block ACTH ‐ induced cortisol secretion. In order to examine this hypothesis, the effects of androgen on ACTH‐induced cortisol secretion in goats were investigated. First, castrated male goats were treated with testosterone (T), DHT or cholesterol (cho) for 21–25 days. Cho was used as a control for T and DHT treatment. Then, plasma cortisol concentrations were compared among the hormonal treatments after ACTH injection. Subsequently, the distribution of androgen receptors in the caprine adrenal gland was investigated. There were no differences in the basal cortisol concentrations among the hormonal treatments. However, plasma cortisol concentrations after ACTH injection were significantly lower in T ‐ and DHT ‐ treated goats than in cho ‐ treated goats. Androgen receptors were present in 60% of the cells in the zonae fasciculata and reticularis of the adrenal cortex, the regions that secrete glucocorticoids. These results suggest that androgen may act directly on the adrenal cortex to suppress cortisol secretion induced by ACTH.  相似文献   

Combined effects of clenbuterol and various concentrations of protein on performance of broiler chickens     

Y. HAMANO  K. KUME  S. YAMAZAKI  S. KOBAYASHI  Y. TERASHIMA 《British poultry science》1998,39(1):117-122

1. The effects of the beta-adrenergic agonist, clenbuterol (1 mg/kg diet) on the growth and muscle composition in female broiler chickens (14 to 32 d of age) fed on diets containing various concentrations of protein (220, 240 or 260 g protein/kg) were examined. 2. Body weight gain over the 18 d period increased linearly with increasing protein intake. The rate of gain was significantly higher in clenbuterol-treated chickens than in control birds. 3. Dietary clenbuterol increased thigh muscle weight and protein concentration of breast and thigh muscle, regardless of dietary protein content. Protein/DNA ratio in thigh muscle was enhanced by clenbuterol feeding, and the magnitude of difference of the ratio was higher in chickens fed on the 240 and 260 g CP/kg diets than in those fed the 220 g CP/kg diet. 4. It was concluded that clenbuterol-treated chickens require increased dietary protein to maintain maximal growth, and that increased protein consumption is an important factor in improving growth in clenbuterolfed broilers.  相似文献