Pharmacokinetic evaluation of ceftiofur in serum, tissue chamber fluid and bronchial secretions from healthy beef-bred calves.          下载免费PDF全文

S L Halstead  R D Walker  J C Baker  R E Holland  G E Stein    J G Hauptman 《Canadian journal of veterinary research》1992,56(4):269-274

Ceftiofur is a new broad spectrum cephalosporin marketed for the treatment of acute bovine respiratory disease. In this investigation ceftiofur was administered by intramuscular injection, at 24 h intervals, to healthy beef-bred calves for four days at dosages of 2.2 and 4.4 mg/kg of body weight, with 4 wk intervals between dosing regimens. Serum, tissue chamber fluid (TCF), and bronchial secretion (BS) concentrations of ceftiofur were measured by microbiological assay after the first and fourth dose of each dosing regimen. Peak serum concentrations (Cmax) of 8.8 micrograms/mL and 17.3 micrograms/mL were obtained approximately 2 h (Tmax), the time of mean peak concentration) after single injections of 2.2 mg/kg and 4.4 mg/kg, respectively. The Cmax was increased approximately twofold following multiple doses of 2.2 mg/kg (Cmax = 13.1 micrograms/mL) and 4.4 mg/kg (Cmax = 24.1 micrograms/mL). Ceftiofur accumulated slowly into TCF and peak concentrations were found to be approximately 14% of those observed in serum after the first dose and approximately 24% after multiple dosing. Concentrations of ceftiofur in BS were obtained rapidly with peak concentrations reaching 45% of the serum Cmax after the first dose. After multiple dosing the Cmax for BS was approximately 25% of the serum Cmax. This study found that both the 2.2 mg/kg and 4.4 mg/kg dosing regimens resulted in continuous serum, TCF and BS concentrations of ceftiofur that exceeded the minimal concentration required to inhibit the bacteria most frequently isolated from calves with acute bovine respiratory disease.  相似文献   

Characterization of staphylococci associated with clinical and subclinical bovine mastitis     

Hodges RT  Jones YS  Holland JT 《New Zealand veterinary journal》1984,32(9):141-145

Attempts were made to identify 900 species of staphylococci or micrococci recovered from samples of bovine milk examined for mastitis pathogens. The presence and identity of haemolysins was recorded together with results of disc diffusion antibiotic sensitivity tests. The occurrence of clinical mastitis was also noted and somatic cell counts (SCC) were performed on milk samples which were normal in appearance. Eight hundred and thirty-one coagulase positive staphylococci were obtained, of which 810 were S. aureus and 21 were S. intermedius. Of 65 coagulase negative staphylococci the species of 19 could not be determined by the identification systems used. The remainder were identified as S. hyicus sub sp. hyicus (1), S. hyicus sub sp. chromogenes (19), S. haemolyticus (17), S. hominis (3), S. epidermidis (4), S. capitis (1) and either S. hominis or S. warneri (1). Four other isolates could not clearly be assigned to the genus Staphylococcus or Micrococcus and were designated irregular strains. No micrococci were identified. The presence of alpha, beta, or delta haemolysins occurring singly or in various combinations was identified in 98.3% of coagulase positive staphylococci and in 60% of coagulase negative staphylococci. Epsilon haemolysin was detected in 47.6% of the coagulase negative staphylococci and in 9.5% of S. intermedius. All staphylococci were sensitive to tetracycline (30 microg), novobiocin (1.6 microg), nafcillin (30 microg), methicillin (10 microg) and cephalothin (30 microg) and variable numbers of each species were sensitive to penicillin (2 iu) and streptomycin (10 microg). One non-identified species of coagulase negative staphylococcus was sensitive to erythromycin (0.4 microg) the remaining staphylococci were resistant. Each of the four irregular strains was sensitive to erythromycin and novobiocin. Clinical mastitis was associated with 30.6% of coagulase positive staphylococci, 15.3% of coagulase negative staphylococci, and two of the four irregular strains (50%). Subclinical mastitis as determined by SCC of 500 x 10(3) or greater was associated with 92.7% of coagulase positive and 37.5% of coagulase negative staphylococci.  相似文献   

Drivers,challenges and opportunities of forage technology adoption by smallholder cattle households in Cambodia     

K Ashley  S Wilson  JR Young  HP Chan  S Vitou  S Suon  PA Windsor  RD Bush 《Tropical animal health and production》2018,50(1):63-73

Forage technology has been successfully introduced into smallholder cattle systems in Cambodia as an alternative feed source to the traditional rice straw and native pastures, improving animal nutrition and reducing labour requirements of feeding cattle. Previous research has highlighted the positive impacts of forage technology including improved growth rates of cattle and household time savings. However, further research is required to understand the drivers, challenges and opportunities of forage technology for smallholder cattle households in Cambodia to facilitate widespread adoption and identify areas for further improvement. A survey of forage-growing households (n = 40) in July–September 2016 examined forage technology adoption experiences, including reasons for forage establishment, use of inputs and labour requirements of forage plot maintenance and use of forages (feeding, fattening, sale of grass or seedlings and silage). Time savings was reported as the main driver of forage adoption with household members spending approximately 1 h per day maintaining forages and feeding it to cattle. Water availability was reported as the main challenge to this activity. A small number of households also reported lack of labour, lack of fencing, competition from natural grasses, cost of irrigation and lack of experience as challenges to forage growing. Cattle fattening and sale of cut forage grass and seedlings was not found to be a widespread activity by interviewed households, with 25 and 10% of households reporting use of forages for these activities, respectively. Currently, opportunities exist for these households to better utilise forages through expansion of forage plots and cattle activities, although assistance is required to support these households in addressing current constraints, particularly availability of water, if the sustainability of this feed technology for smallholder cattle household is to be established in Cambodia.  相似文献   

Abstracts of papers presented at the second otto warburg symposim on biotechnology: plant - microorganism interaction in the rhizosphere     

A. HüTtermann Marie-Therese Oelbe  E. Fritz  G. Schlechte  A. Bytom  A. D. Jungk  Y. Okon  E. Fallik  Rivka Hadas  S. Sarig  E. Yahalom  Sara Tal  A. Hartmann  B. Kishinevsky  Debora Gurfel  Rina Lobel  Y. Kapulnik  D. A. Phillips  Y. Hadar  Y. Chen  E. Jurkevitch  J. Katan  A. Gamliel  H. Ziegler  I. Chet  A. Ordentlich  Hadar Kless  A. Oppenheim  Avia Zilberstein  D. Holland  J. Berman  A. Zamir  R. Kaufman  N. Bawnik  C. Koncz  J. Schell 《Phytoparasitica》1987,15(2):141-147

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Safety, efficacy, and immunogenicity of a modified-live equine influenza virus vaccine in ponies after induction of exercise-induced immunosuppression     

Lunn DP  Hussey S  Sebing R  Rushlow KE  Radecki SV  Whitaker-Dowling P  Youngner JS  Chambers TM  Holland RE  Horohov DW 《Journal of the American Veterinary Medical Association》2001,218(6):900-906

OBJECTIVE: To determine safety, efficacy, and immunogenicity of an intranasal cold-adapted modified-live equine influenza virus vaccine administered to ponies following induction of exercise-induced immunosuppression. DESIGN: Prospective study. ANIMALS: Fifteen 9- to 15-month old ponies that had not had influenza. PROCEDURE: Five ponies were vaccinated after 5 days of strenuous exercise on a high-speed treadmill, 5 were vaccinated without undergoing exercise, and 5 were not vaccinated or exercised and served as controls. Three months later, all ponies were challenged by nebulization of homologous equine influenza virus. Clinical and hematologic responses and viral shedding were monitored, and serum and nasal secretions were collected for determination of influenza-virus-specific antibody isotype responses. RESULTS: Exercise caused immunosuppression, as indicated by depression of lymphocyte proliferation in response to pokeweed mitogen. Vaccination did not result in adverse clinical effects, and none of the vaccinated ponies developed clinical signs of infection following challenge exposure. In contrast, challenge exposure caused marked clinical signs of respiratory tract disease in 4 control ponies. Vaccinated and control ponies shed virus after challenge exposure. Antibody responses to vaccination were restricted to serum IgGa and IgGb responses in both vaccination groups. After challenge exposure, ponies in all groups generated serum IgGa and IgGb and nasal IgA responses. Patterns of serum hemagglutination inhibition titers were similar to patterns of IgGa and IgGb responses. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that administration of this MLV vaccine to ponies with exercise-induced immunosuppression was safe and that administration of a single dose to ponies provided clinical protection 3 months later.  相似文献   

Effects of sodium nitroprusside after load reduction and/or atropine pre-treatment prior to dexmedetomidine administration on cardiovascular variables in dogs     

RD Keegan  SA Greene  BC McKusick 《Veterinary anaesthesia and analgesia》2005,32(4):8-8

The purpose of this study was to determine the cardiovascular effects of sodium nitroprusside (SNP)‐induced after load reduction in dogs administered dexmedetomidine (DEX). Using a randomized crossover design and allowing at least 2 weeks between treatments 12 adult hound dogs of either sex weighing 22 ± 1.7 SD kg were anesthetized by face mask administration of 2.9% ET sevoflurane to facilitate instrumentation prior to administration of treatment drugs. Dogs were intubated and instrumented to enable measurement of heart rate (HR), systolic (SAP), mean (MAP) and diastolic (DAP) arterial pressures, mean pulmonary arterial pressure (PAP), pulmonary capillary wedge pressure (PCWP), central venous pressure (CVP), pulmonary arterial temperature (TEMP), and cardiac output (CO). Systemic (SVR) and pulmonary vascular resistances were calculated. Following completion of instrumentation dogs were allowed to recover for 40 minutes. After collection of baseline data, dogs were administered one of four treatments at T–10 minutes prior to injection of DEX (500? g M^–2 IM): 1) saline (SAL); 2) atropine (ATR, 0.02 [n = 6] or 0.04 [n = 6] mg kg^–1 IM); 3) SAL + SNP (infused at 1–10 ?g kg^–1 minute^–1, IV as needed to maintain MAP between 90–110 mm Hg; or 4) ATR + SNP. Cardiovascular data were collected at T‐20 minutes prior to administration of DEX, T‐5 and at 5, 10, 20, 30, 40, and 60 minutes following DEX. Data were analyzed using anova for repeated measures with post hoc differences between means identified using Bonferroni's method (p < 0.05). Differences in ATR dose were not found to be significant and thus results for ATR dose groups were pooled. Administration of SAL (dexmedetomidine alone) was associated with decreases in HR and CO and increases in SAP, MAP, DAP, CVP, and SVR. Administration of ATR was associated with an increase in HR and CO compared with SAL. Administration of SNP was associated with an increase in HR and CO and a decrease in SVR, MAP and CVP compared with SAL. Administration of SNP + ATR was associated with effects similar to that of SNP or ATR alone and resulted in an additive increase in CO. We conclude that SNP‐induced afterload reduction with or without atropine is effective in mitigating DEX‐induced impairment of cardiovascular function.  相似文献   

A Japanese powdery mildew isolate with exceptionally large infection efficiency on Mlo-resistant barley   总被引：1，自引：2，他引：1  

M. F LYNGKJÆR  H. P JENSEN  H. ØSTERGÅRD 《Plant pathology》1995,44(5):786-790

A Japanese field isolate (Race I) of Erysiphe graminis f.sp, hordei was tested on 17 barley lines carrying the mlo powdery mildew resistance gene. Race I produced many successful infections with infection type larger than or equal to 2 on six lines (M66, MC20, SRI, SR7, A tem and Totem), On the remaining 11 lines it reacted with infection type 0. Colony numbers on the Mlo-lines were between 26% and 12 9% of the numbers on a susceptible cultivar Manchuria, These numbers were larger than, or similar to those produced by isolate HL3/5, which has the highest recorded infection efficiency on Mlo-resistant cultivars. The interaction between isolates and lines was highly significant. The isolate GE3, from which HL3/5 was derived by selection, gave rise to occasional colonies corresponding to less than 03° o of the number produced on cv. Manchuria.  相似文献   

Comparison of mobile and stationary spore-sampling techniques for estimating virulence frequencies in aerial barley powdery mildew populations   总被引：2，自引：0，他引：2  

M. S. HOVMØLLER  L. MUNK  H. ØSTERGÅRD 《Plant pathology》1995,44(5):829-837

Gene frequencies in samples of aerial populations of barley powdery mildew ( Erysiphe graminis f.sp. hordei ), which were collected in adjacent barley areas and in successive periods of time, were compared using mobile and stationary sampling techniques. Stationary samples were collected from trap plants in three periods within 1 week at a distance of more than 1000 m from the nearest barley field. At four dates within the same 8-day period, other samples were collected by a mobile spore trap along four sampling routes of a total distance of 130 km around the stationary stand of exposure. The samples were characterized by virulence genotypes defined according to infection types on 12 near-isogenic barley lines, and frequencies of single virulence genes were subsequently calculated. The three samples collected at the stationary site differed significantly with respect to allele frequencies at three loci. The main wind direction was different in the three sampling periods, implying different powdery mildew sources. For the mobile exposure, the differences between routes were not significant for any locus. However, the routes differed most for the loci under direct selection by host resistances genes, indicating a different distribution of source varieties along routes. There was no difference between allele frequencies at different dates, indicating that the proportions of spores from different source varieties were similar at these dates. In conclusion, samples collected by the stationary technique will mainly reflect the source varieties present in the local area, whereas samples collected by the mobile spore trap will mainly reflect sources close to the sampling route. Therefore, sampling sites as well as sampling routes should be defined such that source varieties are representative for the overall varietal distribution in the survey region considered.  相似文献   

What is your diagnosis? Osteolysis of the cranial aspect of C5 and cranial and caudal vertebral bodies of C6 and C7     

Freshwater R  Holland M 《Journal of the American Veterinary Medical Association》1999,214(3):333-334

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CONTRAST ECHOAORTOGRAPHY AS AN AID IN THE DIAGNOSIS OF RIGHT-TO-LEFT SHUNTING PATENT DUCTUS ARTERIOSUS     

John-Karl  Goodwin  DVM  Merrilee  Holland  DVM  MS 《Veterinary radiology & ultrasound》1995,36(2):157-159

Contrast echocardiography has traditionally been used in conjunction with conventional echocardiography to document right-to-left intracardiac shunting congenital anomalies. This technique does not clearly demonstrate shunting of blood in patients with right-to-left patent ductus arteriosus, as the shunt is extracardiac. We used a variation of contrast echocardiography, contrast echoaortography, to confirm the presence of a right-to-left shunting patent ductus arteriosus in two dogs.  相似文献