Evaluation of an autogenous Salmonella bacterin and a modified live Salmonella serotype Choleraesuis vaccine on a commercial dairy farm.     

J K House  M M Ontiveros  N M Blackmer  E L Dueger  J B Fitchhorn  G R McArthur  B P Smith 《American journal of veterinary research》2001,62(12):1897-1902

OBJECTIVE: To compare the efficacy of a Salmonella bacterin and a modified live Salmonella ser. Choleraesuis vaccine on a commercial dairy. ANIMALS: 450 cows in late gestation and 80 calves. PROCEDURE: Group-1 cows (n = 150) were vaccinated once with a modified live S. Choleraesuis (serogroup C1) strain 54 (SC54) vaccine, group-2 cows (150) were vaccinated on enrollment and 30 days later with a Salmonella ser. Montevideo (serogroup C1) bacterin, and group-3 cows (150) served as unvaccinated controls. One gallon of colostrum harvested from the first 80 cows to calve was fed to each calf. Outcome assessments included fecal shedding of Salmonella spp for the first 10 days after parturition (cows) or birth (calves), milk production, involuntary culling rate, mastitis incidence, antimicrobial use, and mortality rate. RESULTS: Salmonellae were isolated from 306 of 309 (99%) cows and 64 of 74 (86.5%) calves. Shedding frequency was less in SC54-vaccinated cows and calves that received colostrum from those cows, compared with the other groups, and vaccination was specifically associated with less shedding of serogroup C1 salmonellae. Production data were similar among groups. CONCLUSIONS AND CLINICAL RELEVANCE: Vaccination of pregnant cows with an autogenous Salmonella bacterin had no effect on fecal shedding of salmonellae, whereas vaccination with a modified live S. Choleraesuis vaccine reduced the frequency of fecal shedding of serogroup C1 salmonellae during the peripartum period. A commercial S. Choleraesuis vaccine licensed for use in swine may be more efficacious than autogenous Salmonella bacterins on dairies infected with serogroup C1 salmonellae.  相似文献   

Evaluation of a cow‐side milk progesterone assay and assessment of the positive predictive value of oestrus diagnosis by dairy farmers in New South Wales          下载免费PDF全文

L Ingenhoff  E Hall  JK House 《Australian veterinary journal》2016,94(12):445-451

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Variability in concentrations of zinc in serum and feed when using zinc oxide as a supplement for the prevention of facial eczema     

KVA Kannan  WA Mason 《New Zealand veterinary journal》2016,64(6):356-359

AIMS: To determine the variability of concentrations of Zn in feed, when used as a supplement to prevent facial eczema, and to determine the variability in concentrations of Zn in serum between cows and herds that are being supplemented with ZnO in feed, using in-shed feeders or on a feed pad.

METHODS: Sixteen commercial dairy farms in the Waikato region of New Zealand were enrolled, that were supplementing cows with ZnO in the feed using either an automatic in-shed feeder (ASF) or a feed pad (FP) using a feed-out or mixer wagon. On each farm 10 cows were selected by the farmer, that were assumed to be representative of the age and liveweight of the herd. Four hours after supplement feeding, each cow was weighed and a blood sample collected for measurement of concentrations of Zn in serum. Three samples of feed were collected from each farm for Zn analysis, from the beginning, middle and end of the feed being distributed. Levene’s test for homoscedasticity was used to analyse whether there were differences in variation of individual concentrations of Zn in serum, and in the feed, between the two feeding systems. Multivariable linear regression was used to examine associations between age, feeding method or liveweight and concentrations of Zn in serum, after accounting for the variability between farms.

RESULTS: Of the 163 cows sampled, concentrations of Zn in serum were between 20–35?µmol/L in 75/163 (46 (95% CI=38–54)%) cows; were <20?µmol/L in 71/163 (44 (95% CI=36–52)%) cows, and >35?µmol/L in 17/163 (10 (95% CI=6–16)%) cows. The variation in concentrations of Zn in serum in individual cows differed between farms (p<0.001), and the variability was greater for cows fed using a FP than ASF (p<0.001). There was no difference in the variation of concentrations of Zn in feed between the two feeding methods (p=0.54), but concentrations of Zn in serum were associated with the amount of Zn offered in feed (p=0.008).

CONCLUSIONS AND CLINICIAL RELEVENCE: There was significant variability between farms in the concentrations of Zn in the serum of cows being supplemented with ZnO in feed. Only 46% of cows sampled had concentrations of Zn between 20–35?µmol/L. Effective management of facial eczema should include monitoring Zn in the feed and in serum to ensure cows are receiving the correct dose they require.  相似文献   

A framework for evaluation of on-farm mastitis diagnostics in Australia     

RN Zadoks  E Scholz  SM Rowe  JM Norris  HB Pooley  J House 《Australian veterinary journal》2023,101(4):142-152

Numerous culture-based diagnostics are available on the Australian and international markets for on-farm detection of bacterial pathogens in milk. Use of such diagnostics may provide an opportunity to improve the prudent use of antimicrobials in udder health management. Farms are low-resource settings in terms of diagnostic microbiology capacity. The World Health Organisation has identified criteria for the evaluation of diagnostic tests in low resource settings based on Accuracy, Sensitivity, Specificity, User-friendliness, being Rapid or Robust, Equipment-free and being Deliverable (ASSURED). Here, we review how those criteria can be interpreted in the context of microbiological diagnosis of mastitis pathogens, and how on-farm diagnostics that are currently available in Australia perform relative to ASSURED criteria. This evaluation identifies multiple trade-offs, both with regard to scientific criteria and with regards to convenience criteria. More importantly, the purpose of testing may differ between farms, and test performance should be evaluated relative to its intended use. The ability of on-farm mastitis diagnostics to inform mastitis treatment decision-making in a timely and cost-effective manner depends not just on test characteristics but also on farm-specific pathogen prevalence, and on the farm enterprise's priorities and the farm manager's potential courses of action. With most assay evaluations to date conducted in professional laboratories, there is a surprising dearth of information on how well any of the diagnostic tests perform on-farm and, indeed, of the on-farm decision-making processes that they aim to inform.  相似文献   

牧草新品种黔草1号高羊茅的选育   总被引：2，自引：0，他引：2  

吴佳海  牟琼  唐成斌  尚以顺  莫本田  瓦庆荣 《贵州农业科学》2006,34(4):75-79

黔草1号高羊茅是利用贵州地方野生资源作亲本,采用改良混合选择法,经10余年的选育研究育成的新品种。该品种抗逆性强、适应性广、坪用性好、耐践踏、持续性好、绿期长,返青期比引进品种凌志提前7 d,枯黄期晚3 d;种子成熟期集中,种子平均产量比对照品种高20%以上。适应我国长江中下游低山、丘陵等地区种植,是运动场草坪、环境绿化、生态治理的优良草种。  相似文献   

Effect of GnRH pretreatment on reproductive performance of postpartum suckled beef cows following synchronization of estrus using GnRH and PGF2alpha   总被引：1，自引：0，他引：1  

Dejarnette JM  Day ML  House RB  Wallace RA  Marshall CE 《Journal of animal science》2001,79(7):1675-1682

The effect of GnRH pretreatment on estrus detection rate, precision of estrus, and reproductive performance of postpartum beef cows synchronized to estrus using GnRH and PGF2alpha was evaluated. In Exp. 1, Angus cows (n = 87) were randomly assigned by parity, postpartum interval, and body condition score (BCS) to receive either 1) GnRH on d -7 and PGF2alpha on d 0 (GP) or 2) the GP treatment and an additional injection of GnRH on d -16 (GGP). Estrus detection and AI were conducted twice daily from d -3 to d 3. At 72 h after PGF2alpha, all animals not previously detected in estrus were bred by AI and received a concurrent injection of GnRH (TAI). Synchronized pregnancy rates were numerically increased (P = 0.15) in cows treated with GGP (55%) compared with those on the GP treatment (44%). In Exp. 2, 1,276 spring-calving, suckled beef cows in nine herds were randomized to treatments as described for Exp. 1, except that the initial GnRH injection for the GGP treatment was administered on d -14. Herd affected all indicators of reproductive performance (P < 0.05). The percentage of animals detected in estrus prematurely (d -3 to d 0; 7%) was not affected by treatment. Estrus response rate was influenced by postpartum interval (< 60 vs > or = 60; 61 vs 73%; P < 0.01) and a three-way interaction of parity, BCS, and treatment (P < 0.01). Within animals with a BCS > or = 5.5, the GGP treatment tended to increase the detection of estrus in primiparous cows (GP vs GGP; 76 vs 91%; P = 0.11) and decrease detection in multiparous cows (GP vs GGP; 78 vs 72%; P < 0.10). However, because conception rate to TAI in animals with a BCS > or = 5.5 was greater (P < 0.05) in the GGP than in the GP group (28 vs 8%, respectively), this interaction was interpreted to represent a shift in interval to estrus induced by the GGP treatment, rather than a reduction in the synchronization of ovarian function. Conception rates of animals inseminated to an observed estrus did not differ among treatments (P = 0.15). Synchronized pregnancy rate tended (P = 0.06) to be greater in GGP- (53%) than in GP-treated animals (47%). In conclusion, pretreatment with GnRH tended to increase pregnancy rates during a 6-d synchronization period, primarily through enhanced conception rates of cows bred by TAI. In contrast to our hypothesis, GnRH pretreatment did not increase the percentage of animals detected in estrus or the precision of estrus expression.  相似文献   

Firearms--the right balance?     

House C 《The Veterinary record》1999,145(22):647-648

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Prevalence of Salmonella in beef feeder steers as determined by bacterial culture and ELISA serology     

Galland JC  House JK  Hyatt DR  Hawkins LL  Anderson NV  Irwin CK  Smith BP 《Veterinary microbiology》2000,76(2):143-151

Results are presented for monitoring Salmonella infection by bacteriological culture and immune response (enzyme linked immunosorbent assay (ELISA) and haptoglobin) testing of samples collected from beef cattle at a single feedyard sampled over time. A total of 120 beef steers were examined on entry to the feedyard and at days 30, 60, and at time of slaughter (120-150 days). Isolations of Salmonella decreased over time from 40% of the steers sampled at day 0 to 0% at slaughter, whereas serological results varied by serogroup. Seropositivity increased for Salmonella group B up to day 60, and subsequently decreased to about half of the 60-day positivity rate at the time of slaughter. No significant changes in seropositivity were detected during the course of the study for the four other Salmonella serogroups (C1, C3, D1, and E1). Haptoglobin measurements were not a good indicator of Salmonella infection status. Sequential Salmonella testing either by culture, ELISA, or both could be used to monitor pathogen control practices.  相似文献   

Effects of yard weaning and training on the behavioural adaptation of cattle to a feedlot     

K.H. Walker  L.R. Fell  L.A. Reddacliff  R.J. Kilgour  J.R. House  S.C. Wilson  P.J. Nicholls   《Livestock Science》2007,106(2-3):210-217

Two experiments were conducted to determine the effect of weaning in small yards, with or without a feed bunk training procedure, on the subsequent behaviour and performance of Bos taurus steers in a feedlot. A reduction in the incidence of bovine respiratory disease (BRD) was the primary objective. In each experiment, about 200 male beef calves (Angus × Hereford and Hereford) were separated from their mothers at 7–9 months of age and allocated to one of three matched weaning treatment groups. The treatments were (1) yard weaning with hay or silage, (2) yard weaning with hay or silage plus a novel handling procedure to train the animals to be able to find a grain ration in a trough, and (3) paddock weaning without supplement or handling according to common industry practice in southeastern Australia. Paddock weaning is the practice of abrupt separation of cows and calves followed by return to separate pasture paddocks, whereas yard weaning involves abrupt separation but the calves remain in the yards for several days. Experimental vaccines against the major BRD pathogens were given to half of each group 1–2 months prior to entry into a commercial feedlot. The yard weaned and yard trained cattle had a significantly greater weight gain in the first month and over the 90-day feeding period than the paddock weaned control groups. There was no difference between the groups in pre-feedlot weight gain. The yard trained groups showed greater feeding activity during the first few days in the feedlot, but were not significantly different in weight gain from yard weaned. The vaccination treatment also significantly improved the weight gain in the first month and over 80 days. The combination of yard weaning and vaccination produced the highest weight gains overall. There was consistently less morbidity in the yard weaned groups compared to paddock weaned controls. The morbidity in yard trained groups was intermediate between these two. Weaning in small yards and the appropriate use of effective BRD vaccines 1–2 months before feedlot entry are recommended for B. taurus feeder steers in southeastern Australia to minimise sickness and improve productivity in the feedlot. Associated benefits are reduced risks of antibiotic residues and of animal welfare problems.  相似文献   

Evidence of identification of peste des petits ruminants from goats in Egypt     

I M Ismail  J House 《Archiv fuer experimentelle veterinaermedizin》1990,44(3):471-474

Peste des petits ruminants virus was isolated from young goats in an outbreak of the disease for the first time in Egypt. Affected goats showed symptoms simulating rinderpest, disease in cattle. The mortality rate was about 30%, and morbidity reached 90%. History, clinical symptoms, postmortem lesions, and diagnosis were discussed.  相似文献