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1.
Summary Five samples of Aegilops ventricosa (2n=4x=28, genome formula DDNN) from different geographical origins, were crossed in a diallelic scheme. Metaphase I chromosome pairing of the hybrids, accounting for all the possible genetic combinations, was analysed. Only bivalents were formed in some hybrids, while multivalents were scored in other ones. Seed storage proteins, gliadins and albumins, were also analysed by means of polyacrylamide gel electrophoresis. Based on the presence of multivalents in hybrids, and on the differences in seed protein profiles, the samples could be grouped into two clusters. Meiosis was regular in hybrids obtained within samples of the same group, while multivalents were present in hybrids involving a sample of one group and one of the other. The evolutionary trends in Ae. ventricosa are discussed. 相似文献
2.
G. Laghetti A.R. Piergiovanni I. Galasso K. Hammer P. Perrino 《Genetic Resources and Crop Evolution》2000,47(4):461-465
Single-flowered vetch (Vicia articulata Hornem.), a crop native of the Mediterranean area, is widespread mainly in south Europe, in west Asia and Australia for forage, green manure and human food. In Italy this crop was still cultivated in the 1950s but, later, no records concerning its cultivation were available. This strongly suggested to place V. articulata among the Italian crop species that disappeared in the 20th century. In the course of a collecting expedition in Sardinia, a relic population, mistaken for lentil, was found. This population has been characterised from a botanical, nutritional and cytological point of view. V. articulata appears to be lower in nutritional quality than lentil. However, within the framework of a sustainable agriculture, V. articulata could find interesting opportunities. In fact, its role in soil conservation and rescue of marginal areas could be interesting, as well as its potentialities as a source of useful genes in breeding programmes. 相似文献
3.
Lucia Lioi Incoronata Galasso Maria Gloria Daminati Angela Rosa Piergiovanni 《Genetic Resources and Crop Evolution》2010,57(4):533-542
The primary structure of the double-headed Bowman-Birk inhibitor (BBI) family and the antitryptic activity were investigated
in cultivated and wild Phaseolus species. Two BBI types were identified; the first one inhibits trypsin and chymotrypsin (tc-BBI), the second one elastase
and trypsin (et-BBI). Only tc-BBI was found in P. lunatus and P. parvulus, while none of BBI types, identified in this study, was found in P.
leptostachyus. The deduced amino acid sequences revealed some polymorphisms within both tc-BBI and et-BBI binding loops that could affect
the inhibitory activity. The trypsin inhibitor content showed a high variation with the lowest value recorded in P. lepthostachyus and the highest one observed in P. oligospermus. Southern blot analysis confirmed the absence of both BBI types in P. leptostachyus and suggests that in P. coccineus, P. dumosus and P. costaricensis, the two genes were clustered in a narrow genomic region of 1.3 kbp. 相似文献
4.
Digoxigenated synthetic oligonucleotides complementary tosimple repetitive DNA sequences were used to detect polymorphisms inPhaseolus lunatus L. (Lima bean)genotypes. Twenty two accessions, including wild and cultivatedrepresentatives of the Mesoamerican and Andean gene pools andintermediate forms were included in this study. It could be shownthat five repetitive motifs, namely (AAT)5,(ATG)5,(GACA)4,(GATA)4,(GGAT)4, were able to give clear-cutbands when used as probes and to reveal high levels of variation. Thehighest resolving power was revealed by the(AAT)5 probe, which was able to detect eveninter-individual polymorphisms. Therefore, oligonucleotidefingerprinting has proved to be a useful tool to analyse geneticdiversity in Lima bean. Phylogenetic reconstruction based onJACCARD's similarity index confirmed the existence of two majorgene pools and the presence of a minor group of genetically separatedintermediate genotypes. 相似文献
5.
Domenico Pignone Incoronata Galasso Gianfranco Venoral 《Genetic Resources and Crop Evolution》1995,42(1):57-60
Summary Karyomorphological similarities among four species of the genusVigna, V. unguiculata, V. luteola, V. oblongifolia andV. vexillata, were evaluated. Two different approaches were followed: cytomorphometrical determinations, carried out with an automated
image analysis system to improve resolution, yielded some karyotypical indices; the distribution of heterochromatin bands
was interpreted using a recently reported algorithm.
Both methods led to similar results: the most karyologically similar species toV. unguiculata appears to beV. luteola, whileV. oblongifolia appears to be the least related one. 相似文献
6.
Angela Rosa Piergiovanni Incoronata Galasso Pietro Perrino Domenico Pignone 《Genetic Resources and Crop Evolution》1995,42(4):347-352
Summary 27 accessions ofVicia benghalensis from different geographical origins constitute the pool on which the present study was performed. Genetic variation among
the samples was biochemically and cytologically evaluated: seed storage protein profiles and C-banded karyotypes were analysed
from single individuals of each accession.
SDS-PAGE has shown the possibility to divide the samples into two groups, each characterized by specific protein profiles.
The two patterns were indicated as A and B. From individual seed electrophoresis it was ascertained that samples possessing
the pattern A showed a low level of individual variation, while those possessing the pattern B were highly polymorphic, thus
suggesting differences in the allogamic rate.
The cytological analysis demonstrated the presence of two groups of accessions, one being much richer in heterochromatin as
evidenced by C-banding (H+) than the other (H-). The analysis of biochemical and karyological data showed a constant association between pattern A and karyotype H− and between pattern B and karyotype H+.
On the basis of these results it is proposed to considerV. benghalensis as a highly heteromorphic species, in which two groups may be identified. 相似文献
7.
Baron S Finter NB Galasso GJ Glasgow LA Levy HB Younger JS 《Science (New York, N.Y.)》1973,180(4087):779-784
8.
Clementina Sansone Christian Galasso Marco Lo Martire Toms Vega Fernndez Luigi Musco Antonio Dell&#x;Anno Antonino Bruno Douglas M. Noonan Adriana Albini Christophe Brunet 《Marine drugs》2021,19(1)
Marine organisms with fast growth rates and great biological adaptive capacity might have biotechnological interests, since ecological competitiveness might rely on enhanced physiological or biochemical processes’ capability promoting protection, defense, or repair intracellular damages. The invasive seagrass Halophila stipulacea, a non-indigenous species widespread in the Mediterranean Sea, belongs to this category. This is the premise to investigate the biotechnological interest of this species. In this study, we investigated the antioxidant activity in vitro, both in scavenging reactive oxygen species and in repairing damages from oxidative stress on the fibroblast human cell line WI-38. Together with the biochemical analysis, the antioxidant activity was characterized by the study of the expression of oxidative stress gene in WI-38 cells in presence or absence of the H. stipulacea extract. Concomitantly, the pigment pool of the extracts, as well as their macromolecular composition was characterized. This study was done separately on mature and young leaves. Results indicated that mature leaves exerted a great activity in scavenging reactive oxygen species and repairing damages from oxidative stress in the WI-38 cell line. This activity was paralleled to an enhanced carotenoids content in the mature leaf extracts and a higher carbohydrate contribution to organic matter. Our results suggest a potential of the old leaves of H. stipulacea as oxidative stress damage protecting or repair agents in fibroblast cell lines. This study paves the way to transmute the invasive H. stipulacea environmental threat in goods for human health. 相似文献
9.
Simão Zacarias Rodrigo Schveitzer Rafael Arantes Helena Galasso Isabela Pinheiro Carlos Espirito Santo 《Journal Of Applied Aquaculture》2019,31(1):85-96
Effect of K+ and Mg2+ in water on the performance of Litopenaeus vannamei using bioflocs was evaluated. Control had water with K:Mg (1:4.57) and other treatments (K:Mg of 1:5.4, 1:4.65, and 1:3.77), both with salinity of 4 g/L. Initial mean weight of shrimp was 0.08 ± 0.007 g. No difference in shrimp growth performance and survival was observed (P > 0.05) except in the control. Our results suggest that it is possible to culture postlarvae of Litopenaeus vannamei in low-salinity water with bioflocs when the water has an initial potassium concentration of 30.90 ± 8.5 mg/L and magnesium of 167.0 ± 3.9 mg/L. 相似文献
10.
Lucia Lioi Incoronata Galasso Marilina Santantonio Cecilia Lanave Roberto Bollini Francesca Sparvoli 《Genetic Resources and Crop Evolution》2006,53(8):1615-1623
Lectins are a class of defence proteins of non-immune origin that bind carbohydrate in a reversible fashion. In some cultivated
legume species, lectin protein coding genes were PCR amplified using primers designed on the basis of conserved N- and C-terminal
amino acid sequences of the common bean (one-chain) or pea (two-chains) lectins. Amplification products of the expected length
were obtained in Lathyrus sativus L., Vicia faba L. var. faba, Phaseolus coccineus L., and Vigna unguiculata (L.) Walp. No amplification product or agglutinating activity against blood cells, and/or cross-reaction with specific antibodies
were detected in Lupinus albus L. and Cicer arietinum L. Finally, the new isolated nucleotide sequences, together with other legume lectin sequences already present in the EMBL
Database, were used for evolutionary analysis. This last indicated the existence of two main clusters; one grouping all the
species belonging to the Phaseoleae tribe and the other one grouping Lens culinaris Medik., Pisum sativum L., L. sativus, and V. faba, members of the Vicieae tribe. Results were congruent with the taxonomic classification and suggested that the lectin genes
divergence in legume followed species evolution. 相似文献