Identification of internal control genes in milk‐derived mammary epithelial cells during lactation cycle of Indian zebu cow          下载免费PDF全文

Pradeep Jatav  Monika Sodhi  Ankita Sharma  Sandeep Mann  Amit Kishore  Umesh K Shandilya  Ashok K Mohanty  Ranjit S Kataria  Poonam Yadav  Preeti Verma  Surinder Kumar  Dhruba Malakar  Manishi Mukesh 《Animal Science Journal》2016,87(3):344-353

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Segmentation of 3D images of plant tissues at multiple scales using the level set method     

Annamária Kiss  Typhaine Moreau  Vincent Mirabet  Cerasela Iliana Calugaru  Arezki Boudaoud  Pradeep Das 《Plant methods》2017,13(1):114

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Effect of tillage and residue management on properties of two coarse-textured soils and on yield of irrigated wheat and groundnut     

V.K. Bhatnagar  T.N. Chaudhary  B.R. Sharma 《Soil & Tillage Research》1983,3(1):27-37

Changes in soil properties and yield response in relation to tillage and residue management treatments in an irrigated groundnut (Arachis hypogea L.)—wheat (Triticum aestivum, L.) rotation on a sandy soil (1972–1974) and on a sandy loam soil (1974–1976) were evaluated. Tillage treatments (T₁ and T₂) did not cause significant changes in soil properties. Incorporation of crop residues (5 t residue per ha per crop, T₃) caused a substantial increase in organic C, available N, and NaHCO₃ -extractable P contents in the top 15 cm of both soils. Residue management treatments (T₃, T₄ and T₅) showed no significant effect on soil bulk density and exchangeable K. Crop yields under no-tillage (T₁) and conventional tillage (control, T₂) were comparable on sandy soil but on the sandy loam soil, no-tillage yielded significantly lower than conventional tillage. Compared with the control, incorporation of crop residues (T₃) caused higher yields on sandy soil for groundnut and wheat by 34.1 and 47.4%, respectively, and on sandy loam by 17.1 and 7.2%, respectively. Mulching with crop residues conserved soil moisture and reduced maximum soil temperature (1.5–5.3°C in groundnut and 1.5–2.9°C in wheat) but other measured soil properties were not significantly affected. Significant yield increase due to residue mulching (T₄) was observed in sandy soil but it was significantly less than when total crop residue was incorporated (T₃). Increased wheat root-weight density in the top 15 cm soil with residue mulching was not reflected in grain yield. Compared with the incorporation of the total amount of crop residue (T₃), incorporation of half the amount and application of the other half as mulch (T₅) caused lower yields — on sandy soil for groundnut and wheat by 31.5 and 15.7%, respectively, and on sandy loam by 4.8 and 3.6%, respectively.  相似文献   

Papaya yellow leaf curl virus: A newly identified begomovirus infecting Carica papaya L. from the Indian Subcontinent     

Chitra Nehra  Avinash Marwal  Rakesh Kumar Verma  Megha Mishra  Pradeep Sharma 《The Journal of Horticultural Science and Biotechnology》2019,94(4):475-480

India is one of the world’s largest producers of papaya. Viruses, mainly begomoviruses and potyviruses, cause a significant loss in papaya production. The study described here has identified a new species of begomovirus and a new species of betasatellite infecting Carica papaya in India. The sequences of the begomovirus and betasatellite show 90.03% nucleotide sequence identity to an isolate of Radish leaf curl virus and 92.25% identity to an isolate of Tomato leaf curl betasatellite, respectively. Maximum likelihood phylogenetic tree of begomovirus sequence of isolate DP2 (KX353622) showed distant relationships with previously characterised begomoviruses. Recombination analysis proposed six recombination breakpoints in begomovirus genome with other geographical begomovirus isolates.  相似文献   

Application of Azotobacter enhances pond productivity and fish biomass in still water ponds     

S.K. Garg  Anita Bhatnagar  Neeru Narula 《Aquaculture International》1998,6(3):219-231

Two strains (derepressed-nitrogen fixing, Mac-27 and phosphate solubilizing, PS-21) of Azotobacter chroococcum were inoculated in fish culture ponds, singly and in combination with inorganic fertilizers (urea, single superphosphate–SSP). Physico-chemical parameters of pond waters, plankton production and fish biomass were studied. Inoculation of A. chroococcum (Mac-27) enhanced nitrogenase activity and rate of nitrogen fixation. A slight reduction in nitrogen fixation and nitrogenase activity was noticed when urea at 96 kg ha–1 y–1 was mixed with the biofertilizer (Mac-27). Inoculation of PS-21 enhanced phosphate solubilization, but Kjeldahl-nitrogen concentration values remained low in comparison with controls. On the other hand, inoculation of Azotobacter (either strain) enhanced the accumulation of ammonium-N, nitrite-N and nitrate-N. A significant (p < 0.05) reduction in dissolved oxygen (DO) concentration also took place when Azotobacter (both Mac-27 and PS-21) was inoculated in fish ponds. However, when used along with inorganic fertilizers, the reduction was not significant. The pH values were only slightly lowered when the phosphate-solubilizing strain (PS-21) of Azotobacter was inoculated. Inoculation of biofertilizer enhanced plankton production, net primary productivity and fish biomass. However, highest values in most of these parameters were noticed only in ponds that were treated with the higher doses of inorganic fertilizers (urea 192 kg and SSP 1500 kg ha–1 y–1). © Rapid Science Ltd. 1998  相似文献   

Secretion of dopamine and norepinephrine in hypophyseal portal blood and prolactin in peripheral blood of Holstein cattle     

Hard DL  Bhatnagar RK  Molina JR  Anderson LL 《Domestic animal endocrinology》2001,20(2):89-100

The objective was to test the hypothesis that dopamine regulates prolactin (PRL) secretion by determining acute changes in catecholamine concentrations in hypophyseal portal blood of cattle, and their relation to peripheral blood concentration of PRL in hypophyseal stalk-transected (HST) and sham-operated controls (SOC). Holstein heifers (606 +/- 21 kg BW; mean +/- SE) were subjected to neurosurgery for 8 h to collect hypophyseal portal blood with a stainless steel cannula designed with a cuff placed under the pituitary stalk and peripheral blood via a jugular vein catheter. PRL plasma concentration was measured by radioimmunoassay, and dopamine and norepinephrine in portal plasma by radioenzymatic assay. During anesthesia before HST or SOC, PRL plasma concentration ranged from 20-40 ng/ml throughout 255 min. PRL abruptly increased and remained above 90 ng/ml after HST compared with a steady decrease to <20 ng/ml in SOC heifers throughout 440 min. Within 5 min after severing the hypophyseal stalk, dopamine in portal blood (>8 ng/ml) was significantly increased (P < 0.05) compared with peripheral blood (<2 ng/ml). Norepinephrine concentration in portal blood was significantly greater (P < 0.05) than in peripheral blood during the first 60 min. The sustained high PRL level in peripheral plasma after severing the hypophyseal stalk stimulated hypothalamic dopamine secretion from hypophyseal portal vessels during the prolonged period of blood collection. Norepinephrine concentration in these cattle was greater in hypophyseal portal than in peripheral blood, implicating both an important hypothalamic source of the catecholamine as well as an adrenal gland contribution during anesthesia.  相似文献   

A preliminary report of the isolation of a lentogenic strain of Newcastle disease virus (CDF 66) from a pig     

B S Malik  A Bhatnagar  N D Verma 《The British veterinary journal》1969,125(8):Suppl:26-Suppl:28

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Moisture-dependent physical properties of jatropha fruit     

R.C. Pradhan  S.N. Naik  N. Bhatnagar  V.K. Vijay 《Industrial Crops and Products》2009,29(2-3):341-347

The physical properties of fruit are important in designing and fabricating equipment and structures for handling, transporting, processing and storage, and also for assessing quality. The study was conducted to investigate some physical properties of jatropha fruit at various moisture levels. The average length, width, thickness and 1000 mass were 29.31 mm, 22.18 mm, 21.36 mm and 1522.10 g, respectively, at moisture content of 7.97% d.b. The geometric mean diameter increased from 24.03 to 24.70 mm and the sphericity varied between 0.82 and 0.83 as moisture content increased from 7.97% to 23.33% d.b., respectively. In the same moisture range, the bulk and true densities decreased from 278 to 253 and 546 to 435 kg m^?3, respectively, whereas the corresponding porosity also decreased from 49.08% to 41.84%. As the moisture content increased from 7.97% to 23.33% d.b., crushing strength was decreased from 275 to 79 N, whereas the angle of repose and surface areas were found to increase from 36.41° to 41.72° and 1815.73 to 1917.59 mm², respectively. The static coefficient of friction of jatropha fruit increased linearly against the surfaces of three structural materials, namely plywood (47.81%), mild steel (62.88%) and aluminium (34.82%) as the moisture content increased from 7.97% to 23.33% d.b.  相似文献   

Cartilage lesions associated with shaky-leg lameness in turkeys     

R J Julian  M K Bhatnagar 《Avian diseases》1985,29(1):218-232

Shaky-leg lameness is a poorly defined, mild to very severe, painful lameness occurring mainly in 10-to-18-week-old fast-growing male turkeys. Since there are no significant gross or microscopic lesions in early or uncomplicated cases, the cause of the lameness is likely soft-tissue (muscle or tendon) pain. A case history of a flock problem of shaky-leg lameness is described, and it is suggested that stiffness and inactivity secondary to footpad dermatitis in these turkeys was responsible for the severe shaky-leg lameness in the flock. The cartilage lesion described is an avascular lesion and appeared to be the result of inactivity rather than the cause of lameness in these turkeys.  相似文献   

Moisture-dependent physical properties of jatropha seed (Jatropha curcas L.)     

D.K. Garnayak  R.C. Pradhan  S.N. Naik  N. Bhatnagar 《Industrial Crops and Products》2008,27(1):123-129

The study was conducted to investigate some moisture-dependent physical properties of jatropha seed namely, seed dimension, 1000 seed mass, surface area, sphericity, bulk density, true density, angle of repose and static coefficient of friction against different materials. The physical properties of jatropha seed were evaluated as a function of moisture content in the range of 4.75–19.57% d.w. The average length, width, thickness and 1000 seed mass were 18.65 mm, 11.34 mm, 8.91 mm and 741.1 g, respectively at moisture content of 4.75% d.w. The geometric mean diameter and sphericity increased from 12.32 to 12.89 mm and 0.66 to 0.67 as moisture content increased from 4.75 to 19.57% d.w., respectively. In the same moisture range, densities of the rewetted jatropha seed decreased from 492 to 419 kg m⁻³, true density increased from 679 to 767 kg m⁻³, and the corresponding porosity increased from 27.54 to 45.37%. As the moisture content increased from 4.75 to 19.57% d.w., the angle of repose and surface area were found to increase from 28.15° to 39.95° and 476.78 to 521.99 mm², respectively. The static coefficient of friction of jatropha seed increased linearly against the surfaces of three structural materials, namely plywood (44.12%), mild steel sheet (64.15%) and aluminum (68.63%) as the moisture content increased from 4.75 to 19.57% d.w.  相似文献