真核表达载体构建表达ＰＲＶ闽Ａ株gE基因表位抗原编码区片段的重组质粒   总被引：2，自引：1，他引：1  

敖敬群  杨林  王章  娄高明  杜伟贤 《中国预防兽医学报》2001,23(1):14-16

根据伪狂犬病病毒闽Ａ株gE基因表位抗原编码区的序列与身份种真核表达载体pPICZaA、pAcGP67A序列与特性分别设计了两对ＰＣＲ引物。通过ＰＣＲ方法扩增到了两端具有不同酶切位点的gE基因表位抗原编码片段。将这２个片段分别克隆到pPICZaA与pAcGP67A载体，转化大肠杆菌ＴＯＰ１０菌档及ＸＬ１－Ｂlue菌株，获得了含伪狂犬病病毒闽Ａ株gE基因表位抗原编码区的重组质粒pICZaA-FS与pAcGP67A-FS。序测定结果显示两个重组质粒中插入片段的大小与方向均正确。  相似文献   

苦瓜对8种蔬菜的化感作用     

苗朔  敖苏  颜仕龙  袁洋  朱朝华  范志伟 《中国蔬菜》2017,1(2):55

以8种海南主要栽培的蔬菜为受体,采用室内生测法研究苦瓜(Momordica charantiaL.)茎叶水浸提液对其化感作用。结果表明:苦瓜茎叶水浸提液对8种受体植物的种子萌发和幼苗生长均有一定的化感效应,且化感作用方式和强度在受体间及指标间存在显著差异;化感作用综合效应的平均值大小顺序为:辣椒黄瓜豇豆菜豆萝卜樱桃番茄胡萝卜番茄。试验结果为建立合理的栽培制度提供了理论和试验依据。  相似文献   

长白和大白猪主要生长性状的遗传参数估计     

贺婕妤  王斌虎  廖柱  谢红涛  易国强  刘毓文  敖红  唐中林 《畜牧兽医学报》2021,52(8):2115-2123

旨在分析母猪的出生年份、出生季节、初生重、开测日龄等固定效应对长白、大白猪主要生长性状的影响,并对目标生长性状进行遗传参数估计（遗传力、遗传方差、表型相关和遗传相关）,为猪的遗传改良提供基本依据。本试验利用GLM模型分析试验猪群（398头长白猪和1 176头大白猪）的固定效应对猪生长性状的影响,并采用多性状动物模型对目标性状进行遗传参数估计。目标生长性状包括达100 kg体重日龄（age to 100 kg,AGE）、达100 kg背膘厚（backfat to 100 kg,BF）、100 kg平均日增重（average daily gain to 100 kg,ADG）。研究表明,在大白和长白猪中,猪的出生年、出生季、初生重以及开测日龄对生长性状均具有极显著的影响（P<0.001）;长白猪的AGE、ADG和BF的遗传力分别为0.321、0.327和0.324,大白猪对应性状的遗传力分别为0.454、0.469和0.408;长白猪的ADG和AGE之间的遗传相关、表型相关分别为-0.990、-0.995,大白猪的ADG和AGE之间的遗传相关、表型相关分别为-0.993、-0.998,均呈现较强的负相关。长白、大白猪的生长性状（AGE、ADG、BF）均属于中等遗传力性状,其出生年份、出生季节、初生重和开测日龄对猪的生长性状影响较大。在遗传参数估计分析时,提高样本数量并提升表型数据质量,可以增加遗传参数估计的可靠性。本研究中的生长性状遗传参数估计结果较为可靠,可为后续的遗传改良提供参考。  相似文献   

Hemodynamic effects of incremental doses of acepromazine in isoflurane-anesthetized dogs     

Julia P.P. Rangel  Eduardo R. Monteiro  Flavia S. Bitti  Juarez S.N. Junior  Daniela Campagnol 《Veterinary anaesthesia and analgesia》2021,48(2):167-173

ObjectiveTo evaluate the effects of incremental doses of acepromazine on hemodynamics in isoflurane-anesthetized dogs.Study designProspective, experimental study.AnimalsHealthy, adult, mixed-breed dogs (two male and four female) weighing 16.8 ± 5.1 kg (mean ± standard deviation).MethodsDogs were anesthetized with propofol (7 mg kg^–1) intravenously (IV) and isoflurane. Thermodilution and arterial catheters were placed for hemodynamic monitoring and arterial blood sampling for blood gas analysis. Baseline measurements were performed with stable expired concentration of isoflurane (Fe′Iso) at 1.8%. Each dog was then administered four incremental acepromazine injections (10, 15, 25 and 50 μg kg^–1) IV, and measurements were repeated 20 minutes after each acepromazine injection with Fe′Iso decreased to 1.2%. The four acepromazine injections resulted in cumulative doses of 10, 25, 50 and 100 μg kg^–1 (time points ACP₁₀, ACP₂₅, ACP₅₀ and ACP₁₀₀, respectively).ResultsCompared with baseline, cardiac index (CI) increased significantly by 34%, whereas systemic vascular resistance index (SVRI) decreased by 25% at ACP₅₀ and ACP₁₀₀. Arterial oxygen content (CaO₂) was significantly lower than baseline after all acepromazine injections (maximum decreases of 11%) and was lower at ACP₅₀ and ACP₁₀₀ than at ACP₁₀. No significant change was found in heart rate, stroke index, oxygen delivery index and systolic, mean and diastolic blood pressures. Hypotension (mean arterial pressure < 60 mmHg) was observed in one dog at baseline, ACP₁₀, ACP₂₅ and ACP₁₀₀, and in two dogs at ACP₅₀.Conclusions and clinical relevanceCompared with isoflurane alone, anesthesia with acepromazine–isoflurane resulted in increased CI and decreased SVRI and CaO₂ values. These effects were dose-related, being more pronounced at ACP₅₀ and ACP₁₀₀. Under the conditions of this study, acepromazine administration did not change blood pressure.  相似文献   

APSIM模型对宁夏海原地区草田轮作系统的适应性     

奥海玮  谢应忠  李永宏  马建乐 《草地学报》2016,24(1):146-155

为验证APSIM模型对宁夏海原地区草田轮作系统的适用性,基于10年生苜蓿(Medicago sativa)与小麦(Triticum aestivum)、谷子(Oryza sativa)草田轮作试验数据和同期气象资料,运用APSIM模型对系统进行了模拟。通过试错法和文献记载完成了苜蓿、小麦和谷子几个品种的参数本地化。用统计和图形校验方法评价了APSIM模型模拟结果的可靠性和准确性。结果表明,谷子-小麦-小麦(MWW)、小麦-谷子-小麦(WMW)、谷子-谷子-小麦(MMW)、谷子-小麦-谷子(MWM)、小麦-谷子-谷子(WMM)、小麦-小麦-谷子(WWM)6种轮作方式下产量实测值和模拟值的决定系数R2值范围在0.83至0.98之间,D值范围在0.94至0.99之间,表现出了良好的相关性和一致性。土壤含水量实测值和模拟值的决定系数R2值范围在0.52至1之间,D值范围在0.92至0.97之间,相关性和一致性表现良好。表明APSIM模型对宁夏海原地区苜蓿与小麦、谷子轮作具有较好的模拟能力,可以用来模拟分析该地区草田轮作系统生产潜力和土壤水分动态,对该地区气候变化影响下草田轮作的优化管理具有一定指导意义。  相似文献   

基于zigbee技术在矿井下节点切换机制的研究     

赵刚  敖文杰 《湖南农业大学学报(自然科学版)》2015,(1):55-57

节点接入切换机制和路由技术是井下定位系统的关键技术。根据矿井环境的特点，改进移动节点入网接入方式，提出移动节点地址分配的新方案。该方案中移动节点的地址不由协调器分配，在入井前由程序直接设定，且不随位置的移动而改变。仿真结果表明，提高移动节点入网成功率，减少了端到端的延时和丢包率，提高了数据传输效率。  相似文献   

黑珍珠樱桃在黔北地区的生物学特性及栽培技术   总被引：2，自引：2，他引：0  

金方伦  向青云  敖学熙  韩成敏 《贵州农业科学》2010,38(3)

历经7年(2003-2009年)对黔北地区黑珍株樱桃进行了引种观察和栽培试验。结果表明:定植后第2年部分植株开花结果,第3年大部分植株开花结果,第4年植株普遍开花结果,第5～6年以后进入盛果期。植株抗病、抗虫性较强,同时抗低温、抗旱、抗裂果较强。在海拨800～1200m,平地、山地和坡地栽植生长结果均良好。并提出了采用高标准建园、配方施肥、合理修剪、保花保果、合理疏花疏果、有效防治病虫害和合理采收等配套的栽培措施。  相似文献   

Characterization of symptoms of senescence and chilling injury on inflorescences of Heliconia bihai (L.) cv. Lobster Claw and cv. Halloween     

Andreza S. Costa  Luis Carlos Nogueira  Venézio F. dos Santos  Fernando Luiz Finger  Terezinha Rangel Camara  Vivian Loges  Lilia Willadino 《Postharvest Biology and Technology》2011,59(1):103-109

Inadequate temperatures during the shipping and commercialization of cut tropical flowers may accelerate the senescence process and cause chilling injury, leading to symptoms that have not yet been described for Heliconia bihai. The aim of the present study was to evaluate physiological responses in cut inflorescences of H. bihai cv. Lobster Claw (LC) and cv. Halloween (HW) as well as symptoms of senescence and chilling injury. For such, changes in fresh weight, bract color (L*, a* and b*), percentage of absolute integrity (PAI) of cell membranes and leakage of potassium ions (LPI) were determined. The flowering stems were evaluated at five different intervals after harvest (0, 2, 4, 6 and 8 d). A refrigerated treatment (RT) with a temperature of 6.5 °C and 85% relative humidity was compared to a control treatment (CT) at room temperature of 24 °C and 66% relative humidity. Both cultivars stored at 6.5 °C exhibited dryness of bract tissue (symptom of senescence) and dark stains that became brownish and evolved to necrosis (symptom of chilling injury). The visual quality of inflorescences decreased with time in both cultivars maintained without refrigeration. The severity of chilling injury increased with the length of storage time in both cultivars. There was a significant reduction in the fresh weight of inflorescences in both treatments (RT and CT) and both cultivars (LC and HW). Bract color changed in both cultivars at 6.5 °C. There was no change in PAI throughout the evaluation period in the inflorescences stored at room temperature, whereas those stored at 6.5 °C for 6 and 8 d had lower PAI values. The inflorescences in the control treatment underwent no change in LPI values, whereas those stored under refrigeration had increased LPI values after the sixth day of storage. The physiological responses of cut Heliconia flowers were influenced by storage period and temperature, as demonstrated by visual symptoms of chilling injury and senescence.  相似文献   

Cell cycle arrest induced by hypoxia inducible factor-1 alpha in SW626 cell line of human ovarian cancer     

HUANG Lei  AO Qi-lin  LI Fang  XING Hui  LU Yun-ping  LIAO Guo-ning  MA Ding 《园艺学报》2005,21(4):718-721

AIM: To investigate the cell cycle arrest induced by hypoxia, hypoxia inducible factor-1 and their possible mechanism in human ovarian cancer cell line SW626. METHODS: CoCl2, a chemical inducer of hypoxia and hypoxic cell culture chamber were used to induce chemical and physical hypoxia in human ovarian cancer cell line SW626. The method of ‘decoy’ was used to block the function of HIF-1α because it acts as the core sequence of the target gene as a competitor combined to the HIF-1α. The cells were divided into group A1 (normal oxygen), A2 (normal oxygen plus HIF-1α decoy), B1 (CoCl2), B2 (CoCl2 plus HIF-1α decoy), C1 (hypoxia) and C2 (hypoxia plus HIF-1α). The expression of the HIF-1α protein, mRNA and cell cycle analysis were detected by Western blotting, RT-PCR and flow cytometry (FCM). RESULTS: The expression level of HIF-1α protein in group B1 (3.75±1.31) and group C1 (3.48±1.01) was significantly higher than that in group A1 (0.97±0.31) (P<0.05). The expression levels of HIF-1α mRNA in group A1 (0.65±0.32) and group B1 (0.64±0.34) were significantly lower than that in group C1 (1.28±0.62) (P<0.05). Decoy had no effect in the expression of HIF-1α protein and mRNA level (P>0.05). FCM showed that the G0/G1 phase was markedly increased in group B1 (81.78±24.33) and group C1 (77.62±22.76) and was significantly higher than that in group A1 (49.49±18.54) (P<0.05), group B2 (61.54±20.84) was lower than that in group B1 with statistical significance (P<0.05) and group C2 (56.03±21.42) was lower than that in group C1 with statistical significance (P<0.05) , but the difference between group A1 and group A2 (51.77±16.45) had no statistical significance (P>0.05). CONCLUSION: Both CoCl2 and physical hypoxia could distinctly induce cell cycle arrest in G0/G1 phase and the expression of HIF-1α in human ovarian cancer cell line SW626. HIF-1α plays an important role in cell cycle arrest induced by hypoxia in human ovarian cancer cell line SW626.  相似文献   

Molecular characterization of Brazilian isolates of orf virus   总被引：4，自引：0，他引：4  

Mazur C  Ferreira II  Rangel Filho FB  Galler R 《Veterinary microbiology》2000,73(4):253-259

Outbreaks of an epidermic disease suggesting parapox virus infections have been observed in all major herds of sheep and goats from different geographical areas of Brazil. Clinical samples (dried scabs) were collected and orf virus was isolated and characterized by electron microscopy in previous work. In order to characterize these viruses at the molecular level, a modified methodology for genomic DNA extraction directly from scabs was used and such DNA was used to derive the restriction enzyme digestion patterns for clinical samples from three distinct geographic origins. Pulsed field gel electrophoresis was used to separate restriction enzyme DNA fragments and heterogeneity among isolates from different geographic areas could be observed on stained gels. The HindIII-G DNA fragment from orf-A virus genome was cloned and hybridized to DNA of other orf virus isolates. Further heterogeneity was confirmed by these hybridizations.  相似文献