The use of a Giardia vaccine as an immunotherapeutic agent in dogs.          下载免费PDF全文

M E Olson  C J Hannigan  P F Gaviller    L A Fulton 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(11):865-868

Dogs (n = 13), which had failed to be cured of giardiosis following chemotherapeutic measures, were treated with a Giardia vaccine (2-3 injections). Clinical signs resolved between 16 and 42 days postvaccination and cessation of fecal cyst shedding was between 21 and 70 days. Vaccination is a potential method of treating giardiosis in dogs.  相似文献   

Skeletal muscle characteristics in young trained and untrained standardbred trotters.     

M Ronéus  B Essén-Gustavsson  A Lindholm  S G Persson 《Equine veterinary journal》1992,24(4):292-294

Muscle biopsies were taken from the middle gluteal muscle of 28 Standardbred trotters, 3-4 years of age. The 13 horses in Group T were trained consistently from 18 months of age, whereas the 15 horses in Group UT were not exposed to any systematic training before 3 years of age. Group T horses had a lower percentage of Type IIB fibres (31%) than did Group UT horses (39%). Citrate synthase (CS) activity, representing oxidative capacity, was higher in Group T (72 mmol kg-1 min-1) than in Group UT (47 mmol kg-1 min-1). Biopsies were taken from 4 horses in each group when they were foals and then annually until 3-4 years of age. Results from this study indicate that regular training of Standardbreds from 18 months of age resulted in increased CS activity and a decrease in the percentage of Type IIB fibres. This study shows that training, not growth, is the main factor that induces a high oxidative capacity and a high Type IIA/IIB fibre ratio in muscle of Standardbred trotters.  相似文献   

Growth patterns of two lines of Angus cattle selected using predicted growth parameters.     

J J Beltrán  W T Butts  T A Olson  M Koger 《Journal of animal science》1992,70(3):734-741

Linear functions of body weight and condition score at weaning and 18 mo of age were used to predict the mature weight (A) and maturing rate (k) parameters of an asymptotic growth model of Angus cows at the Subtropical Agricultural Research Station, Brooksville, FL. From 1981 through 1988 a heavy-mature-weight line (Line A) and a rapid-maturing line (Line K) were selected based on predicted A and k values. Linear contrasts (A-K) of least squares means for weight at fixed ages indicated that the weight difference between lines increased from birth to maturity during the period of the study. Animals from Line A were heavier (P less than .01) at all ages. A negative response in maternal ability, relative to increased growth potential of their calves, seems to have occurred in the cows of Line A. Mature weight was reached at approximately 4.5 yr of age in Line K and at approximately 5.5 yr in Line A. Brody's three-parameter and Richards' four-parameter functions were fitted to 2,855 quarterly weights of cows, from birth to 6.5 yr of age, to estimate the average growth curve for each line. Brody's model gave better estimates of weights from 18 mo to maturity, but the asymptotic residual mean squares were slightly higher because early weights were overestimated. Linear and nonlinear regression analyses of weight-age data and comparisons of degree of maturity at different premature ages showed differences in the growth patterns of the two lines selected for early predicted values of A and k.  相似文献   

Comparative susceptibility of caponized and uncaponized tom turkeys to Pasteurella multocida.     

R C Friedlander  L D Olson  E L McCune 《Avian diseases》1992,36(1):97-100

When susceptibility to virulent Pasteurella multocida was compared, there was no significant (P greater than 0.05) difference between caponized and uncaponized tom turkeys. Neither was there any significant (P greater than 0.05) difference between the surviving caponized and uncaponized toms in the development of serum anti-P. multocida antibody. However, at 28 weeks of age, the average live body weight of the caponized toms was significantly (P less than 0.05) lower than that of the uncaponized toms. Turkeys were caponized when 9 weeks old, and different groups were exposed to P. multocida when 13, 18, 23, and 28 weeks old.  相似文献   

COLLAGENASE CHEMONUCLEOLYSIS—A LONG TERM RADIOGRAPHIC STUDY IN NORMAL DOGS     

Matthew A.O.  Atilola  DVM  PhD  Joanne R.  Cockshutt  DVM  MS  Ron  Mclaughlin  DVM  DVSc  Susan M.  Cochrane  DVM  DVSc  Paul W.  Pennock  DVM  MS 《Veterinary radiology & ultrasound》1993,34(5):321-324

Five clinically normal five year old dogs were used in this study. From a randomised table inter ver tebral discs were each injected with either collagenase or calcium chloride diluent. The surgically exposed cervical discs were injected with 50 units whereas thoracic and lumbar discs were injected under fluoroscopic guidance with 100 units of the enzyme. Postinjection radiographs revealed significant (p ≤ .05) disc space narrowing in enzyme injected discs. The cervical discs had the highest frequency of radiographic narrowing (87%) followed by the thoracic (70%) and lumbar (53%) discs. Spondylosis deformans developed at the sites of cervical enzyme injections. None of the dogs had neurologic abnormalities one year postinjection.  相似文献   

Biofilm bacteria: formation and comparative susceptibility to antibiotics   总被引：17，自引：0，他引：17       下载免费PDF全文

Merle E. Olson  Howard Ceri  Douglas W. Morck  Andre G. Buret    Ronald R. Read 《Canadian journal of veterinary research》2002,66(2):86-92

The Calgary Biofilm Device (CBD) was used to form bacterial biofilms of selected veterinary gram-negative and gram-positive pathogenic bacteria from cattle, sheep, pigs, chicken, and turkeys. The minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) of ampicillin, ceftiofur, cloxacillin, oxytetracycline, penicillin G, streptomycin, tetracycline, enrofloxacin, erythromycin, gentamicin, tilmicosin, and trimethoprim-sulfadoxine for gram-positive and -negative bacteria were determined. Bacterial biofilms were readily formed on the CBD under selected conditions. The biofilms consisted of microcolonies encased in extracellular polysaccharide material. Biofilms composed of Arcanobacterium (Actinomyces) pyogenes, Staphylococcus aureus, Staphylococcus hyicus, Streptococcus agalactiae, Corynebacterium renale, or Corynebacterium pseudotuberculosis were not killed by the antibiotics tested but as planktonic bacteria they were sensitive at low concentrations. Biofilm and planktonic Streptococcus dysgalactiae and Streptococcus suis were sensitive to penicillin, ceftiofur, cloxacillin, ampicillin, and oxytetracycline. Planktonic Escherichia coli were sensitive to enrofloxacin, gentamicin, oxytetracycline and trimethoprim/ sulfadoxine. Enrofloxacin and gentamicin were the most effective antibiotics against E. coli growing as a biofilm. Salmonella spp. and Pseudomonas aeruginosa isolates growing as planktonic populations were sensitive to enrofloxacin, gentamicin, ampicillin, oxytetracycline, and trimethoprim/sulfadoxine, but as a biofilm, these bacteria were only sensitive to enrofloxacin. Planktonic and biofilm Pasteurella multocida and Mannheimia haemolytica had similar antibiotic sensitivity profiles and were sensitive to most of the antibiotics tested. The CBD provides a valuable new technology that can be used to select antibiotics that are able to kill bacteria growing as biofilms.  相似文献   

Presence of bacterial glycocalyx and fimbriae on Pasteurella haemolytica in feedlot cattle with pneumonic pasteurellosis.   总被引：2，自引：1，他引：1       下载免费PDF全文

D W Morck  M E Olson  S D Acres  P Y Daoust    J W Costerton 《Canadian journal of veterinary research》1989,53(2):167-171

This investigation was conducted to determine if Pasteurella haemolytica within feedlot cattle affected by pneumonic pasteurellosis express fimbriae (pili) and bacterial glycocalyx. Bacteriological culture of pulmonary tissue from three calves with fibrinous pneumonia resulted in heavy growth of P. haemolytica. Transmission electron microscopy of the lungs showed numerous microcolonies of gram-negative bacteria with morphology typical of Pasteurella haemolytica. The cells within these microcolonies possessed bacterial glycocalyces which stained with ruthenium red. Glycocalyx-encased microcolonies were also present in specimens examined by scanning electron microscopy. Typical P. haemolytica cells were evident in a tracheal specimen and these bacteria had radial glycocalyces consistent with polysaccharide and proteinaceous material condensed on linear structures suggestive of fimbriae. The pathogenetic importance of the bacterial glycocalyx and fimbriae in shipping fever pneumonia has yet to be established but their presence in clinical cases of Pasteurella pneumonia in feedlot cattle further supports a possible role in the initiation and progression of this disease as well as bacterial resistance to antimicrobial agents.  相似文献   

The parasympatholytic effects of atropine sulfate and glycopyrrolate in rats and rabbits.          下载免费PDF全文

M E Olson  D Vizzutti  D W Morck    A K Cox 《Canadian journal of veterinary research》1994,58(4):254-258

Nine groups of rats (n = 5 per group) received an intramuscular (IM) injection of one of the following drugs or drug combinations: saline, atropine (0.05 mg/kg), glycopyrrolate (0.5 mg/kg), ketamine:xylazine (85:15 mg/kg), ketamine:detomidine (60:10 mg/kg), atropine:ketamine:xylazine (0.05: 85:15 mg/kg), glycopyrrolate: ketamine:xylazine (0.5:85:15 mg/kg), atropine:ketamine:detomidine (0.05: 60:10 mg/kg) or glycopyrrolate: ketamine:detomidine (0.5:60:10). Similarly six groups of rabbits (n = 5) received an IM injection of either saline, atropine (0.2 mg/kg), atropine (2 mg/kg), glycopyrrolate (0.1 mg/kg), ketamine:xylazine (35:10 mg/kg) or glycopyrrolate:ketamine:xylazine (0.1:35:10 mg/kg). In rats, atropine sulfate (0.05 mg/kg) and glycopyrrolate (0.5 mg/kg) produced an increase in heart rate for 30 and 240 min, respectively. In rabbits atropine sulfate at either 0.2 or 2.0 mg/kg did not induce a significant increase in heart rate, but glycopyrrolate (0.1 mg/kg) elevated the heart rate above saline treated animals for over 50 min. Both atropine and glycopyrrolate provided protection against a decrease in heart rate in rats anesthetized with ketamine: xylazine (85:15 mg/kg) or ketamine: detomidine (60:10 mg/kg); however, glycopyrrolate was significantly more effective in maintaining the heart rate within the normal range. Glycopprrolate also prevented a decrease in heart rate in rabbits anesthetized with ketamine:xylazine (35:5 mg/kg). Neither glycopyrrolate nor atropine influenced respiration rate, core body temperature or systolic blood pressure when used alone or when combined with the injectable anesthetic. Glycopyrrolate is an effective anticholinergic agent in rabbits and rodents and more useful as a preanesthetic agent than atropine sulfate in these animals.  相似文献   

A comparison of polymerase chain reaction with and without RNA extraction and virus isolation for detection of bovine viral diarrhea virus in young calves.     

D Deregt  P S Carman  R M Clark  K M Burton  W O Olson  S A Gilbert 《Journal of veterinary diagnostic investigation》2002,14(5):433-437

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Book reviews     

Ron G. Board  M. H. Maxwell  A. H. Sykes 《British poultry science》1991,32(4):885-888

The Avian Egg: Chemistry & Biology, by R. W. Burley and D. V. Vadehra, 1989. v+472 pp. illustrated. New York. John Wiley & Sons. £66.70. ISBN 0–471–849952.

A Colour Atlas of Avian Anatomy, by J. McLelland. 1990. 127 pp. illustrated. London, Wolfe Publishing Ltd. £30. ISBN 0–7234–1575–7.

The Tropical Agriculturalist—Poultry, by A. J. Smith, 1990. vi + 218 pp. CTA/Macmillan, London. ISBN 0–333–52306–7.  相似文献